Strain Name:

B6.129-Ido1tm1Alm/J

Stock Number:

005867

Order this mouse

Availability:

Repository- Live

Other products are available, see Purchasing Information for Cryopreserved Embryos

Use Restrictions Apply, see Terms of Use
The lack of endogenous protein in homozygotes of these indoleamine 2,3-dioxygenase 1 knock-out mice affects immune and a number of other physiological responses. These mice may be useful in studies of pregnancy and reproductive immunology, as well as other immune response and physiological processes.

Description

Strain Information

Former Names B6.129-Indotm1Alm/J    (Changed: 14-JAN-09 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   16-JAN-07
Specieslaboratory mouse
GenerationN10+N1pF17 (11-DEC-13)
Generation Definitions
 
Donating Investigator Andrew Mellor,   Medical College of Georgia

Description
Homozygous mice are viable and fertile with normal immune system development and function. They exhibit no spontaneous autoimmune disorders. No gene product (mRNA or protein) from the targeted gene is detected in the epididymis. At embryonic day 10.5, endogenous protein is absent from all cells at the maternal-fetal interface when both parents are homozygous for the targeted gene. Allogeneic and syngeneic pregnancy outcomes are unaffected by this mutation. In contrast to wild-type, anti-proliferative treatments (CTLA4-Ig, IFNalpha, or CpG-ODN) do not suppress T cell expansion both in vivo and in vitro. In addition, homozygous dendritic cells isolated from lymph nodes draining (induced) tumor sites have no suppressor activity. These mice may be useful in studies of pregnancy and reproductive immunology (tryptophan degradation, T cell activation, clonal expansion) as well as autoimmune disease, tissue transplantation, fostering, acquired tolerance/T cell anergy, and immunosuppressive pathways.

Development
A targeting vector was designed to replace exons 3-5 (encode critical portions of the enzyme catalytic site) with the beta-galactosidase and neomycin resistance genes. Additionally, a translational "stop" codon (TAG) was introduced into exon 2. The construct was electroporated into 129/SvJ-derived embryonic stem (ES) cells and correctly targeted clones were injected into blastocysts. Male chimeric mice were mated with C57BL/6 females to produce heterozygotes. Mutant mice were backcrossed more than 10 generations to C57BL/6 before arriving at The Jackson Laboratory.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Ido1tm1Alm allele
012427   C.129X1(B6)-Ido1tm1Alm/J
View Strains carrying   Ido1tm1Alm     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ido1tm1Alm/Ido1tm1Alm

        either: (involves: 129X1/SvJ * C57BL/6) or (involves: 129X1/SvJ * CBA)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • no effect on reproductive success   (MGI Ref ID J:93797)

Ido1tm1Alm/Ido1tm1Alm

        involves: 129X1/SvJ * BALB/c
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • males exhibit normal fertility and testis weight   (MGI Ref ID J:170565)
    • abnormal caput epididymis morphology
      • levels of some cytokines are higher in mutant caput epididymis   (MGI Ref ID J:170565)
      • significantly greater amounts of protein in caput epididymis extracts than in controls   (MGI Ref ID J:170565)
      • lysosomal autophagy is seen in mutant but not wild-type caput epididymal tissues   (MGI Ref ID J:170565)
    • abnormal sperm number
      • caudal epididymal sperm counts are nearly twice as high in mutants as in wild-type males   (MGI Ref ID J:170565)
    • epididymal inflammation
      • whereas wild-type caput epididymis exhibits a high KYN/Trp ratio indicating that it is in an immunosuppressed state, mutants have a much lower KYN/Trp ratio, indicating that the caput epididymis is in an inflammatory state   (MGI Ref ID J:170565)
    • teratozoospermia
      • increase in the proportion of spermatozoa exhibiting abnormal morphology, including pinhead, giant head, hairpin and kite spermatozoa   (MGI Ref ID J:170565)
      • enlarged sperm head   (MGI Ref ID J:170565)
      • hairpin sperm flagellum   (MGI Ref ID J:170565)
      • pinhead sperm   (MGI Ref ID J:170565)
  • cellular phenotype
  • abnormal autophagy
    • lysosomal autophagy is seen in mutant but not wild-type caput epididymal tissues as indicated by an increase in multilamellar/multivesicular bodies   (MGI Ref ID J:170565)
  • hairpin sperm flagellum   (MGI Ref ID J:170565)
  • hematopoietic system phenotype
  • decreased leukocyte cell number
    • decrease in leukocyte count in caudal epididymal fluid   (MGI Ref ID J:170565)
  • homeostasis/metabolism phenotype
  • abnormal protein level
    • significantly greater amounts of protein in caput epididymis extracts than in controls due to decreased protein degradation   (MGI Ref ID J:170565)
    • abnormal cytokine level
      • levels of some cytokines are higher in mutant caput epididymis   (MGI Ref ID J:170565)
  • immune system phenotype
  • abnormal cytokine level
    • levels of some cytokines are higher in mutant caput epididymis   (MGI Ref ID J:170565)
  • decreased leukocyte cell number
    • decrease in leukocyte count in caudal epididymal fluid   (MGI Ref ID J:170565)
  • epididymal inflammation
    • whereas wild-type caput epididymis exhibits a high KYN/Trp ratio indicating that it is in an immunosuppressed state, mutants have a much lower KYN/Trp ratio, indicating that the caput epididymis is in an inflammatory state   (MGI Ref ID J:170565)

Ido1tm1Alm/Ido1tm1Alm

        involves: 129X1/SvJ
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • tryptophan plasma levels are normal   (MGI Ref ID J:177903)

Ido1tm1Alm/Ido1tm1Alm

        involves: 129X1/SvJ * C57BL/6J * FVB/N
  • immune system phenotype
  • decreased susceptibility to type IV hypersensitivity reaction
    • reduced ear swelling in a contact hypersensitivity assay with oxazolone   (MGI Ref ID J:211949)
  • increased interferon-gamma secretion
    • in a contact hypersensitivity assay with oxazolone   (MGI Ref ID J:211949)
    • however, lymph node cells treated with PMA and ionomycin exhibit normal secretion   (MGI Ref ID J:211949)
  • increased interleukin-6 secretion
    • in a contact hypersensitivity assay with oxazolone   (MGI Ref ID J:211949)
    • however, lymph node cells treated with PMA and ionomycin exhibit normal secretion   (MGI Ref ID J:211949)
  • increased tumor necrosis factor secretion
    • in a contact hypersensitivity assay with oxazolone   (MGI Ref ID J:211949)
    • however, lymph node cells treated with PMA and ionomycin exhibit normal secretion   (MGI Ref ID J:211949)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Immunodeficiency
      T cell deficiency

Research Tools
Cancer Research
      B and T cell deficiency, xenograft/transplant host
Immunology, Inflammation and Autoimmunity Research
      T cell deficiency, xenograft/transplant host
Reproductive Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ido1tm1Alm
Allele Name targeted mutation 1, Andrew L Mellor
Allele Type Targeted (Null/Knockout)
Common Name(s) IDO-; Ido1-KO; Ido1-;
Mutation Made By Brendan Marshall,   Medical College of Georgia
Strain of Origin129X1/SvJ
ES Cell Line Strain129
Gene Symbol and Name Ido1, indoleamine 2,3-dioxygenase 1
Chromosome 8
Gene Common Name(s) IDO; IDO-1; INDO; Ido; Indo; indole 2,3-dioxygenase; indole 2,4-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase;
Molecular Note A Betagal and neomycin cassette replaced exons 3-5, which encode critical portions of the enzyme catalytic site. Also, a translation stop codon was introduced into exon 2. RT-PCR of total epididymis RNA from mutants showed no transcript was present. Western blot of mutant epididymis confirmed a lack of protein. [MGI Ref ID J:93797]

Genotyping

Genotyping Information

Genotyping Protocols

Ido1tm1Alm, Melt Curve Analysis
Ido1tm1Alm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Baban B; Chandler P; McCool D; Marshall B; Munn DH; Mellor AL. 2004. Indoleamine 2,3-dioxygenase expression is restricted to fetal trophoblast giant cells during murine gestation and is maternal genome specific. J Reprod Immunol 61(2):67-77. [PubMed: 15063630]  [MGI Ref ID J:93797]

Additional References

Ido1tm1Alm related

Baban B; Chandler PR; Sharma MD; Pihkala J; Koni PA; Munn DH; Mellor AL. 2009. IDO activates regulatory T cells and blocks their conversion into Th17-like T cells. J Immunol 183(4):2475-83. [PubMed: 19635913]  [MGI Ref ID J:151475]

Baban B; Hansen AM; Chandler PR; Manlapat A; Bingaman A; Kahler DJ; Munn DH; Mellor AL. 2005. A minor population of splenic dendritic cells expressing CD19 mediates IDO-dependent T cell suppression via type I IFN signaling following B7 ligation. Int Immunol 17(7):909-19. [PubMed: 15967784]  [MGI Ref ID J:99971]

Bessede A; Gargaro M; Pallotta MT; Matino D; Servillo G; Brunacci C; Bicciato S; Mazza EM; Macchiarulo A; Vacca C; Iannitti R; Tissi L; Volpi C; Belladonna ML; Orabona C; Bianchi R; Lanz TV; Platten M; Della Fazia MA; Piobbico D; Zelante T; Funakoshi H; Nakamura T; Gilot D; Denison MS; Guillemin GJ; DuHadaway JB; Prendergast GC; Metz R; Geffard M; Boon L; Pirro M; Iorio A; Veyret B; Romani L; Grohmann U; Fallarino F; Puccetti P. 2014. Aryl hydrocarbon receptor control of a disease tolerance defence pathway. Nature 511(7508):184-90. [PubMed: 24930766]  [MGI Ref ID J:213564]

Blumenthal A; Nagalingam G; Huch JH; Walker L; Guillemin GJ; Smythe GA; Ehrt S; Britton WJ; Saunders BM. 2012. M. tuberculosis induces potent activation of IDO-1, but this is not essential for the immunological control of infection. PLoS One 7(5):e37314. [PubMed: 22649518]  [MGI Ref ID J:187311]

Bonifazi P; D'Angelo C; Zagarella S; Zelante T; Bozza S; De Luca A; Giovannini G; Moretti S; Iannitti RG; Fallarino F; Carvalho A; Cunha C; Bistoni F; Romani L. 2010. Intranasally delivered siRNA targeting PI3K/Akt/mTOR inflammatory pathways protects from aspergillosis. Mucosal Immunol 3(2):193-205. [PubMed: 19924119]  [MGI Ref ID J:194658]

Centuori SM; Trad M; LaCasse CJ; Alizadeh D; Larmonier CB; Hanke NT; Kartchner J; Janikashvili N; Bonnotte B; Larmonier N; Katsanis E. 2012. Myeloid-derived suppressor cells from tumor-bearing mice impair TGF-beta-induced differentiation of CD4+CD25+FoxP3+ Tregs from CD4+CD25-FoxP3- T cells. J Leukoc Biol 92(5):987-97. [PubMed: 22891289]  [MGI Ref ID J:189810]

Cobbold SP; Adams E; Farquhar CA; Nolan KF; Howie D; Lui KO; Fairchild PJ; Mellor AL; Ron D; Waldmann H. 2009. Infectious tolerance via the consumption of essential amino acids and mTOR signaling. Proc Natl Acad Sci U S A 106(29):12055-60. [PubMed: 19567830]  [MGI Ref ID J:150809]

Fukunaga M; Yamamoto Y; Kawasoe M; Arioka Y; Murakami Y; Hoshi M; Saito K. 2012. Studies on tissue and cellular distribution of indoleamine 2,3-dioxygenase 2: the absence of IDO1 upregulates IDO2 expression in the epididymis. J Histochem Cytochem 60(11):854-60. [PubMed: 22895526]  [MGI Ref ID J:214281]

Harrington L; Srikanth CV; Antony R; Rhee SJ; Mellor AL; Shi HN; Cherayil BJ. 2008. Deficiency of indoleamine 2,3-dioxygenase enhances commensal-induced antibody responses and protects against Citrobacter rodentium-induced colitis. Infect Immun 76(7):3045-53. [PubMed: 18426872]  [MGI Ref ID J:137302]

Holmgaard RB; Zamarin D; Munn DH; Wolchok JD; Allison JP. 2013. Indoleamine 2,3-dioxygenase is a critical resistance mechanism in antitumor T cell immunotherapy targeting CTLA-4. J Exp Med 210(7):1389-402. [PubMed: 23752227]  [MGI Ref ID J:201770]

Hoshi M; Matsumoto K; Ito H; Ohtaki H; Arioka Y; Osawa Y; Yamamoto Y; Matsunami H; Hara A; Seishima M; Saito K. 2012. L-tryptophan-kynurenine pathway metabolites regulate type I IFNs of acute viral myocarditis in mice. J Immunol 188(8):3980-7. [PubMed: 22422885]  [MGI Ref ID J:184065]

Hoshi M; Saito K; Hara A; Taguchi A; Ohtaki H; Tanaka R; Fujigaki H; Osawa Y; Takemura M; Matsunami H; Ito H; Seishima M. 2010. The absence of IDO upregulates type I IFN production, resulting in suppression of viral replication in the retrovirus-infected mouse. J Immunol 185(6):3305-12. [PubMed: 20693424]  [MGI Ref ID J:163841]

Huang L; Li L; Klonowski KD; Tompkins SM; Tripp RA; Mellor AL. 2013. Induction and role of indoleamine 2,3 dioxygenase in mouse models of influenza a virus infection. PLoS One 8(6):e66546. [PubMed: 23785507]  [MGI Ref ID J:203475]

Ito H; Hoshi M; Ohtaki H; Taguchi A; Ando K; Ishikawa T; Osawa Y; Hara A; Moriwaki H; Saito K; Seishima M. 2010. Ability of IDO to attenuate liver injury in alpha-galactosylceramide-induced hepatitis model. J Immunol 185(8):4554-60. [PubMed: 20844202]  [MGI Ref ID J:164734]

Jasperson LK; Bucher C; Panoskaltsis-Mortari A; Mellor AL; Munn DH; Blazar BR. 2009. Inducing the tryptophan catabolic pathway, indoleamine 2,3-dioxygenase (IDO), for suppression of graft-versus-host disease (GVHD) lethality. Blood 114(24):5062-70. [PubMed: 19828695]  [MGI Ref ID J:155467]

Jasperson LK; Bucher C; Panoskaltsis-Mortari A; Taylor PA; Mellor AL; Munn DH; Blazar BR. 2008. Indoleamine 2,3-dioxygenase is a critical regulator of acute graft-versus-host disease lethality. Blood 111(6):3257-65. [PubMed: 18077788]  [MGI Ref ID J:132719]

Jrad-Lamine A; Henry-Berger J; Damon-Soubeyrand C; Saez F; Kocer A; Janny L; Pons-Rejraji H; Munn DH; Mellor AL; Gharbi N; Cadet R; Guiton R; Aitken RJ; Drevet JR. 2013. Indoleamine 2,3-dioxygenase 1 (ido1) is involved in the control of mouse caput epididymis immune environment. PLoS One 8(6):e66494. [PubMed: 23840489]  [MGI Ref ID J:204333]

Jrad-Lamine A; Henry-Berger J; Gourbeyre P; Damon-Soubeyrand C; Lenoir A; Combaret L; Saez F; Kocer A; Tone S; Fuchs D; Zhu W; Oefner PJ; Munn DH; Mellor AL; Gharbi N; Cadet R; Aitken RJ; Drevet JR. 2011. Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state. J Biol Chem 286(10):8030-42. [PubMed: 21189261]  [MGI Ref ID J:170565]

Jung ID; Lee MG; Chang JH; Lee JS; Jeong YI; Lee CM; Park WS; Han J; Seo SK; Lee SY; Park YM. 2009. Blockade of indoleamine 2,3-dioxygenase protects mice against lipopolysaccharide-induced endotoxin shock. J Immunol 182(5):3146-54. [PubMed: 19234212]  [MGI Ref ID J:146236]

Kanai M; Funakoshi H; Takahashi H; Hayakawa T; Mizuno S; Matsumoto K; Nakamura T. 2009. Tryptophan 2,3-dioxygenase is a key modulator of physiological neurogenesis and anxiety-related behavior in mice. Mol Brain 2:8. [PubMed: 19323847]  [MGI Ref ID J:177903]

Kim H; Chen L; Lim G; Sung B; Wang S; McCabe MF; Rusanescu G; Yang L; Tian Y; Mao J. 2012. Brain indoleamine 2,3-dioxygenase contributes to the comorbidity of pain and depression. J Clin Invest 122(8):2940-54. [PubMed: 22751107]  [MGI Ref ID J:190079]

Lee J; Lee J; Park MK; Lim MA; Park EM; Kim EK; Yang EJ; Lee SY; Jhun JY; Park SH; Kim HY; Cho ML. 2013. Interferon gamma suppresses collagen-induced arthritis by regulation of Th17 through the induction of indoleamine-2,3-deoxygenase. PLoS One 8(4):e60900. [PubMed: 23613752]  [MGI Ref ID J:200571]

Lu Y; Giver CR; Sharma A; Li JM; Darlak KA; Owens LM; Roback JD; Galipeau J; Waller EK. 2012. IFN-gamma and indoleamine 2,3-dioxygenase signaling between donor dendritic cells and T cells regulates graft versus host and graft versus leukemia activity. Blood 119(4):1075-85. [PubMed: 22130799]  [MGI Ref ID J:181750]

Maazi H; Shirinbak S; den Boef LE; Fallarino F; Volpi C; Nawijn MC; van Oosterhout AJ. 2013. Cytotoxic T lymphocyte antigen 4-immunoglobulin G is a potent adjuvant for experimental allergen immunotherapy. Clin Exp Immunol 172(1):113-20. [PubMed: 23480191]  [MGI Ref ID J:194897]

Manlapat AK; Kahler DJ; Chandler PR; Munn DH; Mellor AL. 2007. Cell-autonomous control of interferon type I expression by indoleamine 2,3-dioxygenase in regulatory CD19+ dendritic cells. Eur J Immunol 37(4):1064-71. [PubMed: 17343295]  [MGI Ref ID J:120813]

Masoumy M; Yu J; Liu JY; Yanasak N; Middleton C; Lamoke F; Mozaffari MS; Baban B. 2014. The role of indoleamine 2,3 dioxygenase in beneficial effects of stem cells in hind limb ischemia reperfusion injury. PLoS One 9(4):e95720. [PubMed: 24752324]  [MGI Ref ID J:215178]

Mazarei G; Budac DP; Lu G; Lee H; Moller T; Leavitt BR. 2013. The absence of indoleamine 2,3-dioxygenase expression protects against NMDA receptor-mediated excitotoxicity in mouse brain. Exp Neurol 249:144-8. [PubMed: 23994717]  [MGI Ref ID J:203830]

Mellor AL; Baban B; Chandler P; Marshall B; Jhaver K; Hansen A; Koni PA; Iwashima M; Munn DH. 2003. Cutting edge: induced indoleamine 2,3 dioxygenase expression in dendritic cell subsets suppresses T cell clonal expansion. J Immunol 171(4):1652-5. [PubMed: 12902462]  [MGI Ref ID J:93796]

Mellor AL; Baban B; Chandler PR; Manlapat A; Kahler DJ; Munn DH. 2005. Cutting edge: CpG oligonucleotides induce splenic CD19+ dendritic cells to acquire potent indoleamine 2,3-dioxygenase-dependent T cell regulatory functions via IFN Type 1 signaling. J Immunol 175(9):5601-5. [PubMed: 16237046]  [MGI Ref ID J:119367]

Mellor AL; Chandler P; Baban B; Hansen AM; Marshall B; Pihkala J; Waldmann H; Cobbold S; Adams E; Munn DH. 2004. Specific subsets of murine dendritic cells acquire potent T cell regulatory functions following CTLA4-mediated induction of indoleamine 2,3 dioxygenase. Int Immunol 16(10):1391-401. [PubMed: 15351783]  [MGI Ref ID J:93639]

Merlo LM; Pigott E; DuHadaway JB; Grabler S; Metz R; Prendergast GC; Mandik-Nayak L. 2014. IDO2 is a critical mediator of autoantibody production and inflammatory pathogenesis in a mouse model of autoimmune arthritis. J Immunol 192(5):2082-90. [PubMed: 24489090]  [MGI Ref ID J:209940]

Metz R; Smith C; DuHadaway JB; Chandler P; Baban B; Merlo LM; Pigott E; Keough MP; Rust S; Mellor AL; Mandik-Nayak L; Muller AJ; Prendergast GC. 2014. IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation. Int Immunol 26(7):357-67. [PubMed: 24402311]  [MGI Ref ID J:211949]

Mohib K; Wang S; Guan Q; Mellor AL; Sun H; Du C; Jevnikar AM. 2008. Indoleamine 2,3-dioxygenase expression promotes renal ischemia-reperfusion injury. Am J Physiol Renal Physiol 295(1):F226-34. [PubMed: 18480171]  [MGI Ref ID J:148645]

Muller AJ; DuHadaway JB; Jaller D; Curtis P; Metz R; Prendergast GC. 2010. Immunotherapeutic suppression of indoleamine 2,3-dioxygenase and tumor growth with ethyl pyruvate. Cancer Res 70(5):1845-53. [PubMed: 20160032]  [MGI Ref ID J:158003]

Muller AJ; Sharma MD; Chandler PR; Duhadaway JB; Everhart ME; Johnson BA 3rd; Kahler DJ; Pihkala J; Soler AP; Munn DH; Prendergast GC; Mellor AL. 2008. Chronic inflammation that facilitates tumor progression creates local immune suppression by inducing indoleamine 2,3 dioxygenase. Proc Natl Acad Sci U S A 105(44):17073-8. [PubMed: 18952840]  [MGI Ref ID J:144863]

Nagano J; Shimizu M; Hara T; Shirakami Y; Kochi T; Nakamura N; Ohtaki H; Ito H; Tanaka T; Tsurumi H; Saito K; Seishima M; Moriwaki H. 2013. Effects of indoleamine 2,3-dioxygenase deficiency on high-fat diet-induced hepatic inflammation. PLoS One 8(9):e73404. [PubMed: 24039933]  [MGI Ref ID J:207350]

Noh KT; Chae SH; Chun SH; Jung ID; Kang HK; Park YM. 2013. Resveratrol suppresses tumor progression via the regulation of indoleamine 2,3-dioxygenase. Biochem Biophys Res Commun 431(2):348-53. [PubMed: 23291179]  [MGI Ref ID J:198077]

O'Connor JC; Lawson MA; Andre C; Briley EM; Szegedi SS; Lestage J; Castanon N; Herkenham M; Dantzer R; Kelley KW. 2009. Induction of IDO by bacille Calmette-Guerin is responsible for development of murine depressive-like behavior. J Immunol 182(5):3202-12. [PubMed: 19234218]  [MGI Ref ID J:146234]

Ohtaki H; Ito H; Ando K; Ishikawa T; Hoshi M; Tanaka R; Osawa Y; Yokochi T; Moriwaki H; Saito K; Seishima M. 2009. Interaction between LPS-induced NO production and IDO activity in mouse peritoneal cells in the presence of activated Valpha14 NKT cells. Biochem Biophys Res Commun 389(2):229-34. [PubMed: 19715679]  [MGI Ref ID J:154077]

Pallotta MT; Orabona C; Volpi C; Vacca C; Belladonna ML; Bianchi R; Servillo G; Brunacci C; Calvitti M; Bicciato S; Mazza EM; Boon L; Grassi F; Fioretti MC; Fallarino F; Puccetti P; Grohmann U. 2011. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Nat Immunol 12(9):870-8. [PubMed: 21804557]  [MGI Ref ID J:176469]

Peng K; Monack DM. 2010. Indoleamine 2,3-dioxygenase 1 is a lung-specific innate immune defense mechanism that inhibits growth of Francisella tularensis tryptophan auxotrophs. Infect Immun 78(6):2723-33. [PubMed: 20385761]  [MGI Ref ID J:160167]

Ravishankar B; Liu H; Shinde R; Chandler P; Baban B; Tanaka M; Munn DH; Mellor AL; Karlsson MC; McGaha TL. 2012. Tolerance to apoptotic cells is regulated by indoleamine 2,3-dioxygenase. Proc Natl Acad Sci U S A 109(10):3909-14. [PubMed: 22355111]  [MGI Ref ID J:182142]

Riella LV; Dada S; Chabtini L; Smith B; Huang L; Dakle P; Mfarrej B; D'Addio F; Adams LT; Kochupurakkal N; Vergani A; Fiorina P; Mellor AL; Sharpe AH; Yagita H; Guleria I. 2013. B7h (ICOS-L) maintains tolerance at the fetomaternal interface. Am J Pathol 182(6):2204-13. [PubMed: 23578385]  [MGI Ref ID J:198478]

Rytelewski M; Meilleur CE; Yekta MA; Szabo PA; Garg N; Schell TD; Jevnikar AM; Sharif S; Singh B; Haeryfar SM. 2014. Suppression of immunodominant antitumor and antiviral CD8+ T cell responses by indoleamine 2,3-dioxygenase. PLoS One 9(2):e90439. [PubMed: 24587363]  [MGI Ref ID J:214465]

Sharma MD; Baban B; Chandler P; Hou DY; Singh N; Yagita H; Azuma M; Blazar BR; Mellor AL; Munn DH. 2007. Plasmacytoid dendritic cells from mouse tumor-draining lymph nodes directly activate mature Tregs via indoleamine 2,3-dioxygenase. J Clin Invest 117(9):2570-82. [PubMed: 17710230]  [MGI Ref ID J:127409]

Sharma MD; Hou DY; Liu Y; Koni PA; Metz R; Chandler P; Mellor AL; He Y; Munn DH. 2009. Indoleamine 2,3-dioxygenase controls conversion of Foxp3+ Tregs to TH17-like cells in tumor-draining lymph nodes. Blood 113(24):6102-11. [PubMed: 19366986]  [MGI Ref ID J:149490]

Smith C; Chang MY; Parker KH; Beury DW; DuHadaway JB; Flick HE; Boulden J; Sutanto-Ward E; Soler AP; Laury-Kleintop LD; Mandik-Nayak L; Metz R; Ostrand-Rosenberg S; Prendergast GC; Muller AJ. 2012. IDO is a nodal pathogenic driver of lung cancer and metastasis development. Cancer Discov 2(8):722-35. [PubMed: 22822050]  [MGI Ref ID J:193156]

Sumpter TL; Dangi A; Matta BM; Huang C; Stolz DB; Vodovotz Y; Thomson AW; Gandhi CR. 2012. Hepatic stellate cells undermine the allostimulatory function of liver myeloid dendritic cells via STAT3-dependent induction of IDO. J Immunol 189(8):3848-58. [PubMed: 22962681]  [MGI Ref ID J:190536]

Takamatsu M; Hirata A; Ohtaki H; Hoshi M; Hatano Y; Tomita H; Kuno T; Saito K; Hara A. 2013. IDO1 plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice. J Immunol 191(6):3057-64. [PubMed: 23956437]  [MGI Ref ID J:205871]

Tawara I; Shlomchik WD; Jones A; Zou W; Nieves E; Liu C; Toubai T; Duran-Struuck R; Sun Y; Clouthier SG; Evers R; Lowler KP; Levy RB; Reddy P. 2010. A crucial role for host APCs in the induction of donor CD4+CD25+ regulatory T cell-mediated suppression of experimental graft-versus-host disease. J Immunol 185(7):3866-72. [PubMed: 20810991]  [MGI Ref ID J:164307]

Vinay DS; Kim CH; Choi BK; Kwon BS. 2009. Origins and functional basis of regulatory CD11c+CD8+ T cells. Eur J Immunol 39(6):1552-63. [PubMed: 19499519]  [MGI Ref ID J:149436]

Wang Y; Liu H; McKenzie G; Witting PK; Stasch JP; Hahn M; Changsirivathanathamrong D; Wu BJ; Ball HJ; Thomas SR; Kapoor V; Celermajer DS; Mellor AL; Keaney JF Jr; Hunt NH; Stocker R. 2010. Kynurenine is an endothelium-derived relaxing factor produced during inflammation. Nat Med 16(3):279-85. [PubMed: 20190767]  [MGI Ref ID J:158753]

Xu H; Oriss TB; Fei M; Henry AC; Melgert BN; Chen L; Mellor AL; Munn DH; Irvin CG; Ray P; Ray A. 2008. Indoleamine 2,3-dioxygenase in lung dendritic cells promotes Th2 responses and allergic inflammation. Proc Natl Acad Sci U S A 105(18):6690-5. [PubMed: 18436652]  [MGI Ref ID J:134645]

Zelante T; Iannitti RG; Cunha C; De Luca A; Giovannini G; Pieraccini G; Zecchi R; D'Angelo C; Massi-Benedetti C; Fallarino F; Carvalho A; Puccetti P; Romani L. 2013. Tryptophan catabolites from microbiota engage aryl hydrocarbon receptor and balance mucosal reactivity via interleukin-22. Immunity 39(2):372-85. [PubMed: 23973224]  [MGI Ref ID J:208224]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes or homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   16-JAN-07
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Ido1tm1Alm  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Ido1tm1Alm x Homozygous for Ido1tm1Alm  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Ido1tm1Alm  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Ido1tm1Alm x Homozygous for Ido1tm1Alm  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)