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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation ?+N1F6 (05-AUG-08) Donating Investigator Andrew Mellor, Medical College of Georgia Description
Homozygous mice are viable and fertile with normal immune system development and function. They exhibit no spontaneous autoimmune disorders. No gene product (mRNA or protein) from the targeted gene is detected in the epididymis. At embryonic day 10.5, endogenous protein is absent from all cells at the maternal-fetal interface when both parents are homozygous for the targeted gene. Allogeneic and syngeneic pregnancy outcomes are unaffected by this mutation. In contrast to wildtype, anti-proliferative treatments (CTLA4-Ig, IFNalpha, or CpG-ODN) do not suppress T cell expansion both in vivo and in vitro. In addition, homozygous dendritic cells isolated from lymph nodes draining (induced) tumor sites have no suppressor activity. These mice may be useful in studies of pregnancy and reproductive immunology (tryptophan degradation, T cell activation, clonal expansion) as well as autoimmune disease, tissue transplantation, fostering, acquired tolerance/T cell anergy, and immunosuppressive pathways.Development
A targeting vector was designed to replace exons 3-5 (encode critical portions of the enzyme catalytic site) with the beta-galactosidase and neomycin resistance genes. Additionally, a translational "stop" codon (TAG) was introduced into exon 2. The construct was electroporated into 129/SvJ-derived embryonic stem (ES) cells and correctly targeted clones were injected into blastocysts. Male chimeric mice were mated with C57BL/6 females to produce heterozygotes. Mutant mice were backcrossed more than 10 generations to C57BL/6 before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Indotm1Alm/Indotm1Alm
either: (involves: 129X1/SvJ * C57BL/6) or (involves: 129X1/SvJ * CBA)
- reproductive system phenotype
- *normal* reproductive system phenotype (MGI Ref ID J:93797)
- no effect on reproductive success
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Autoimmunity
Immunodeficiency (T cell deficiency)
Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Immunology and Inflammation Research (T cell deficiency) (xenograft/transplant host)
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Indotm1Alm | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Andrew L Mellor | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | IDO-; | ||
| Mutation Made By | Brendan Marshall, Medical College of Georgia | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Indo, indoleamine-pyrrole 2,3 dioxygenase | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | CD107B; IDO; IDO1; Ido; indole 2,3-dioxygenase; indole 2,4-dioxygenase; | ||
| Molecular Note | A Betagal and neomycin cassette replaced exons 3-5, which encode critical portions of the enzyme catalytic site. Also, a translation stop codon was introduced into exon 2. RT-PCR of total epididymis RNA from mutants showed no transcript was present. Western blot of mutant epididymis confirmed a lack of protein. [MGI Ref ID J:93797] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Indotm1Alm, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Baban B; Chandler P; McCool D; Marshall B; Munn DH; Mellor AL. 2004. Indoleamine 2,3-dioxygenase expression is restricted to fetal trophoblast giant cells during murine gestation and is maternal genome specific. J Reprod Immunol 61(2):67-77. [PubMed: 15063630] [MGI Ref ID J:93797]
Additional References
Indotm1Alm relatedBaban B; Hansen AM; Chandler PR; Manlapat A; Bingaman A; Kahler DJ; Munn DH; Mellor AL. 2005. A minor population of splenic dendritic cells expressing CD19 mediates IDO-dependent T cell suppression via type I IFN signaling following B7 ligation. Int Immunol 17(7):909-19. [PubMed: 15967784] [MGI Ref ID J:99971]
Harrington L; Srikanth CV; Antony R; Rhee SJ; Mellor AL; Shi HN; Cherayil BJ. 2008. Deficiency of indoleamine 2,3-dioxygenase enhances commensal-induced antibody responses and protects against Citrobacter rodentium-induced colitis. Infect Immun 76(7):3045-53. [PubMed: 18426872] [MGI Ref ID J:137302]
Jasperson LK; Bucher C; Panoskaltsis-Mortari A; Taylor PA; Mellor AL; Munn DH; Blazar BR. 2008. Indoleamine 2,3-dioxygenase is a critical regulator of acute graft-versus-host disease lethality. Blood 111(6):3257-65. [PubMed: 18077788] [MGI Ref ID J:132719]
Manlapat AK; Kahler DJ; Chandler PR; Munn DH; Mellor AL. 2007. Cell-autonomous control of interferon type I expression by indoleamine 2,3-dioxygenase in regulatory CD19+ dendritic cells. Eur J Immunol 37(4):1064-71. [PubMed: 17343295] [MGI Ref ID J:120813]
Mellor AL; Baban B; Chandler P; Marshall B; Jhaver K; Hansen A; Koni PA; Iwashima M; Munn DH. 2003. Cutting edge: induced indoleamine 2,3 dioxygenase expression in dendritic cell subsets suppresses T cell clonal expansion. J Immunol 171(4):1652-5. [PubMed: 12902462] [MGI Ref ID J:93796]
Mellor AL; Baban B; Chandler PR; Manlapat A; Kahler DJ; Munn DH. 2005. Cutting edge: CpG oligonucleotides induce splenic CD19+ dendritic cells to acquire potent indoleamine 2,3-dioxygenase-dependent T cell regulatory functions via IFN Type 1 signaling. J Immunol 175(9):5601-5. [PubMed: 16237046] [MGI Ref ID J:119367]
Mellor AL; Chandler P; Baban B; Hansen AM; Marshall B; Pihkala J; Waldmann H; Cobbold S; Adams E; Munn DH. 2004. Specific subsets of murine dendritic cells acquire potent T cell regulatory functions following CTLA4-mediated induction of indoleamine 2,3 dioxygenase. Int Immunol 16(10):1391-401. [PubMed: 15351783] [MGI Ref ID J:93639]
Xu H; Oriss TB; Fei M; Henry AC; Melgert BN; Chen L; Mellor AL; Munn DH; Irvin CG; Ray P; Ray A. 2008. Indoleamine 2,3-dioxygenase in lung dendritic cells promotes Th2 responses and allergic inflammation. Proc Natl Acad Sci U S A 105(18):6690-5. [PubMed: 18436652] [MGI Ref ID J:134645]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as heterozygotes or homozygotes. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.60 Female or Male Homozygous for Indotm1Alm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.20 Homozygous for Indotm1Alm x Homozygous for Indotm1Alm
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.30 Female or Male Homozygous for Indotm1Alm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.60 Homozygous for Indotm1Alm x Homozygous for Indotm1Alm
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”No Warranty
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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.