Strain Name:

NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ

Stock Number:

005868

Availability:

Repository- Live

Common Names: 8.3-TCR-alpha/beta transgenic NOD;     NOD-TCRalphabeta-TG;     8.3-NOD;     NOD.8.3;     NY8.3-NOD;    

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
H2 Haplotypeg7
Generation[?+F2N1p]+F5N1F1 (23-JAN-08)
 
Donating Investigator David Serreze,   The Jackson Laboratory

Appearance
pink-eyed, albino

Related Genotype: A/A Tyrc/Tyrc

Description
NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ, commonly called 8.3-NOD, express rearranged Tcra and Tcrb transgenes derived from the pancreatic beta cell-cytotoxic CD8+ T cell clone NY8.3. CD4-CD8+ thymocytes and lymph derived T cells are skewed toward VB8.1/2+ expression when compared to wild type controls. Although, transgenic mice exhibit dramatically accelerated diabetes, the cumulative diabetes incidence and kinetics of disease are remarkably similar to their wild type cohorts. Insulitis scores of 3 week old transgene+ mice was significantly lower, while insulitis scores of 6 week olds were significantly more severe than in wild types controls, Verdaguer J et al, 1997, J. Exp Med 186, 1663-1676.
Transgenic animals bearing both TCR transgenes offer a source of CTL precursors useful in examining the diversity of beta cell peptides recognized by the autoreactive CD8+ T lymphocytes contributing to the earliest phase of IDDM development.

Development
(SJLXC57BL/6J)F2 transgenic mice were backcrossed to NOD. In 2005, the Type 1 Diabetes Resource received congenic NOD hemizygous transgenic mice backcrossed at least 14 generations to NOD.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tcra
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003199   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J
002116   B6.129S2-Tcratm1Mom/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
007962   B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J
002115   B6;129S2-Tcratm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
004194   C57BL/6-Tg(TcraTcrb)425Cbn/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
007080   CByJ.B6-Tg(TcraTcrb)1100Mjb/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
004444   NOD.129P2(C)-Tcratm1Mjo/DoiJ
006436   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcraAI4)1Dvs/DvsJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/Dvs
004259   NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+
004347   NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi/DoiJ
006303   NOD.FVB-Tg(TcraBDC12-4.1)10Jos/GseJ
004334   NOD/ShiLt-Tg(TcraAI4)1Dvs
003868   NOD/ShiLt-Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+
002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcra     (35 strains)

Strains carrying other alleles of Tcrb
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003200   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRB)C14Jg/J
002122   B6.129P2-Tcrbtm1Mom Tcrdtm1Mom/J
002118   B6.129P2-Tcrbtm1Mom/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
007962   B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J
002121   B6;129P-Tcrbtm1Mom Tcrdtm1Mom/J
002117   B6;129P2-Tcrbtm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
004194   C57BL/6-Tg(TcraTcrb)425Cbn/J
003540   C57L/J-Tg(Tcrb)93Vbo/J
003447   CBy.129P2(B6)-Tcrbtm1Mom/SzJ
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
007081   CByJ.129P2(B6)-Tcrbtm1Mom/J
007080   CByJ.B6-Tg(TcraTcrb)1100Mjb/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
006437   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcrbAI4)1Dvs/DvsJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/Dvs
004259   NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+
004348   NOD.Cg-Rag1tm1Mom Tg(TcrbAI4)1Dvs/DvsJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi/DoiJ
006304   NOD.FVB-Tg(TcrbBDC12-4.1)82Gse/GseJ
003868   NOD/ShiLt-Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+
004335   NOD/ShiLt-Tg(TcrbAI4)1Dvs
002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcrb     (39 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(TcraTcrbNY8.3)1Pesa/?

        involves: C57BL/6 * NOD * SJL
  • immune system phenotype
  • pancreas inflammation (MGI Ref ID J:44202)
    • insulitis at 3 weeks less than in controls
    • insulitis at 6 weeks of age significantly greater than in controls
  • homeostasis/metabolism phenotype
  • increased circulating glucose level (MGI Ref ID J:44202)
    • diabetes develops in 26% (males) to 33% (females) of mice
    • diabetes slow to develop and moderate
  • endocrine/exocrine gland phenotype
  • pancreas inflammation (MGI Ref ID J:44202)
    • insulitis at 3 weeks less than in controls
    • insulitis at 6 weeks of age significantly greater than in controls
  • digestive/alimentary phenotype
  • pancreas inflammation (MGI Ref ID J:44202)
    • insulitis at 3 weeks less than in controls
    • insulitis at 6 weeks of age significantly greater than in controls
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes

Research Tools
Diabetes and Obesity Research
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Tcra related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Research Tools
Cancer Research (specific T cell deficiency)

Tcrb related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(TcraTcrbNY8.3)1Pesa
Allele Name transgene insertion 1, Pere Santamaria
Allele Type Transgenic (random, expressed)
Common Name(s) 8.3-TCR; 8.3-TCR-TG; 8.3-TCR-alpha/beta transgene; 8.3TCR; NY8.3; Tcr8.3;
Mutation Made By Pere Santamaria,   University of Calgary
Strain of Origin(SJL x C57BL/6)F2
Expressed Gene Tcrb, T-cell receptor beta chain, mouse, laboratory
Expressed Gene Tcra, T-cell receptor alpha chain, mouse, laboratory
Molecular Note Functional VDJbeta and VJalpha rearrangements were isolated from the beta cell cytotoxic CD8(+) T cell clone NY8.3. Upstream regualtory sequence together with either TCR-beta enhancers or TCR-alpha enhancers, as appropriate, were associated with VDJbetaor VJalpha. [MGI Ref ID J:44202]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Verdaguer J; Schmidt D; Amrani A; Anderson B; Averill N; Santamaria P. 1997. Spontaneous autoimmune diabetes in monoclonal T cell nonobese diabetic mice. J Exp Med 186(10):1663-76. [PubMed: 9362527]  [MGI Ref ID J:44202]

Additional References

Tg(TcraTcrbNY8.3)1Pesa related

Amrani A; Serra P; Yamanouchi J; Han B; Thiessen S; Verdaguer J; Santamaria P. 2002. CD154-dependent priming of diabetogenic CD4(+) T cells dissociated from activation of antigen-presenting cells. Immunity 16(5):719-32. [PubMed: 12049723]  [MGI Ref ID J:76802]

Amrani A; Verdaguer J; Anderson B; Utsugi T; Bou S; Santamaria P. 1999. Perforin-independent beta-cell destruction by diabetogenic CD8(+) T lymphocytes in transgenic nonobese diabetic mice. J Clin Invest 103(8):1201-9. [PubMed: 10207172]  [MGI Ref ID J:108737]

Chong MM; Chen Y; Darwiche R; Dudek NL; Irawaty W; Santamaria P; Allison J; Kay TW; Thomas HE. 2004. Suppressor of cytokine signaling-1 overexpression protects pancreatic beta cells from CD8+ T cell-mediated autoimmune destruction. J Immunol 172(9):5714-21. [PubMed: 15100317]  [MGI Ref ID J:89692]

DiLorenzo TP; Lieberman SM; Takaki T; Honda S; Chapman HD; Santamaria P; Serreze DV; Nathenson SG. 2002. During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. Clin Immunol 105(3):332-41. [PubMed: 12498815]  [MGI Ref ID J:94192]

Dudek NL; Thomas HE; Mariana L; Sutherland RM; Allison J; Estella E; Angstetra E; Trapani JA; Santamaria P; Lew AM; Kay TW. 2006. Cytotoxic T-cells from T-cell receptor transgenic NOD8.3 mice destroy beta-cells via the perforin and Fas pathways. Diabetes 55(9):2412-8. [PubMed: 16936188]  [MGI Ref ID J:116592]

Fallarino F; Grohmann U; You S; McGrath BC; Cavener DR; Vacca C; Orabona C; Bianchi R; Belladonna ML; Volpi C; Santamaria P; Fioretti MC; Puccetti P. 2006. The combined effects of tryptophan starvation and tryptophan catabolites down-regulate T cell receptor zeta-chain and induce a regulatory phenotype in naive T cells. J Immunol 176(11):6752-61. [PubMed: 16709834]  [MGI Ref ID J:131797]

Graham KL; Sanders N; Tan Y; Allison J; Kay TW; Coulson BS. 2008. Rotavirus infection accelerates type 1 diabetes in mice with established insulitis. J Virol 82(13):6139-49. [PubMed: 18417562]  [MGI Ref ID J:138082]

Han B; Serra P; Yamanouchi J; Amrani A; Elliott JF; Dickie P; Dilorenzo TP; Santamaria P. 2005. Developmental control of CD8 T cell-avidity maturation in autoimmune diabetes. J Clin Invest 115(7):1879-87. [PubMed: 15937548]  [MGI Ref ID J:99646]

Judkowski V; Krakowski M; Rodriguez E; Mocnick L; Santamaria P; Sarvetnick N. 2004. Increased islet antigen presentation leads to type-1 diabetes in mice with autoimmune susceptibility. Eur J Immunol 34(4):1031-40. [PubMed: 15048713]  [MGI Ref ID J:88883]

Krishnamurthy B; Dudek NL; McKenzie MD; Purcell AW; Brooks AG; Gellert S; Colman PG; Harrison LC; Lew AM; Thomas HE; Kay TW. 2006. Responses against islet antigens in NOD mice are prevented by tolerance to proinsulin but not IGRP. J Clin Invest 116(12):3258-65. [PubMed: 17143333]  [MGI Ref ID J:117352]

Krishnamurthy B; Mariana L; Gellert SA; Colman PG; Harrison LC; Lew AM; Santamaria P; Thomas HE; Kay TW. 2008. Autoimmunity to Both Proinsulin and IGRP Is Required for Diabetes in Nonobese Diabetic 8.3 TCR Transgenic Mice. J Immunol 180(7):4458-4464. [PubMed: 18354167]  [MGI Ref ID J:132969]

Leiter EH; Reifsnyder P; Driver J; Kamdar S; Choisy-Rossi C; Serreze DV; Hara M; Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab 9 Suppl 2:14-22. [PubMed: 17919174]  [MGI Ref ID J:127015]

Serra P; Amrani A; Yamanouchi J; Han B; Thiessen S; Utsugi T; Verdaguer J; Santamaria P. 2003. CD40 ligation releases immature dendritic cells from the control of regulatory CD4+CD25+ T cells. Immunity 19(6):877-89. [PubMed: 14670304]  [MGI Ref ID J:86995]

Thomas HE; Irawaty W; Darwiche R; Brodnicki TC; Santamaria P; Allison J; Kay TW. 2004. IL-1 Receptor Deficiency Slows Progression to Diabetes in the NOD Mouse. Diabetes 53(1):113-121. [PubMed: 14693705]  [MGI Ref ID J:87251]

Ueno A; Cho S; Cheng L; Wang J; Hou S; Nakano H; Santamaria P; Yang Y. 2007. Transient upregulation of indoleamine 2,3-dioxygenase in dendritic cells by human chorionic gonadotropin downregulates autoimmune diabetes. Diabetes 56(6):1686-93. [PubMed: 17360980]  [MGI Ref ID J:126514]

Verdaguer J; Amrani A; Anderson B; Schmidt D; Santamaria P. 1999. Two mechanisms for the non-MHC-linked resistance to spontaneous autoimmunity. J Immunol 162(8):4614-26. [PubMed: 10202001]  [MGI Ref ID J:109898]

Wang J; Cho S; Ueno A; Cheng L; Xu BY; Desrosiers MD; Shi Y; Yang Y. 2008. Ligand-dependent induction of noninflammatory dendritic cells by anergic invariant NKT cells minimizes autoimmune inflammation. J Immunol 181(4):2438-45. [PubMed: 18684934]  [MGI Ref ID J:140188]

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778]  [MGI Ref ID J:120349]

Zwicker KA; Gratton KJ; Santamaria P; Bathe OF. 2003. Tumor immunity in the context of autoimmunity. J Surg Res 114(2):274. [PubMed: 14559559]  [MGI Ref ID J:86058]

de Jersey J; Snelgrove SL; Palmer SE; Teteris SA; Mullbacher A; Miller JF; Slattery RM. 2007. Beta cells cannot directly prime diabetogenic CD8 T cells in nonobese diabetic mice. Proc Natl Acad Sci U S A 104(4):1295-300. [PubMed: 17229843]  [MGI Ref ID J:119514]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB29

Colony Maintenance

Breeding & HusbandryFACS analysis, utlizing VB8.1,2,3 FITC antibody costaining with CD8a PE, is used to genotype mice from this strain.

The accelerated diabetes development in NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ hemizygous breeding stock is not significantly retarded by Complete Freund's Adjuvant.

Mating System+/+ sibling x Hemizygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $54.00Female or MaleHemizygous for Tg(TcraTcrbNY8.3)1Pesa
Pairs /Price*Pair Genotype
$108.00Hemizygous for Tg(TcraTcrbNY8.3)1Pesa x Hemizygous for Tg(TcraTcrbNY8.3)1Pesa
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $70.20Female or MaleHemizygous for Tg(TcraTcrbNY8.3)1Pesa
Pairs /Price*Pair Genotype
$140.40Hemizygous for Tg(TcraTcrbNY8.3)1Pesa x Hemizygous for Tg(TcraTcrbNY8.3)1Pesa
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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phone:207-288-6470
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(3.2)