Strain Name:

NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ

Stock Number:

005868

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Availability:

Repository- Live

Common Names: 8.3-TCR-alpha/beta transgenic NOD;     NOD-TCRalphabeta-TG;     8.3-NOD;     NOD.8.3;     NY8.3-NOD;    
NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ, commonly called 8.3-NOD, express rearranged Tcra and Tcrb transgenes derived from the pancreatic beta cell-cytotoxic CD8+ T cell clone NY8.3. Transgenic animals bearing both TCR transgenes offer a source of CTL precursors useful in examining the diversity of beta cell peptides recognized by the autoreactive CD8+ T lymphocytes contributing to the earliest phase of diabetes mellitus type 1 (IDDM) development.

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating System+/+ sibling x Hemizygote         (Female x Male)   02-MAY-08
Specieslaboratory mouse
H2 Haplotypeg7
Generation?+N1pN6 (28-OCT-13)
Generation Definitions
 
Donating InvestigatorDr. David Serreze,   The Jackson Laboratory

Appearance
pink-eyed, albino

Related Genotype: A/A Tyrc/Tyrc

Description
NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ, commonly called 8.3-NOD, express rearranged Tcra and Tcrb transgenes derived from the pancreatic beta cell-cytotoxic CD8+ T cell clone NY8.3. CD4-CD8+ thymocytes and lymph derived T cells are skewed toward VB8.1/2+ expression when compared to wild type controls. Although, transgenic mice exhibit dramatically accelerated diabetes, the cumulative diabetes incidence and kinetics of disease are remarkably similar to their wild type cohorts. Insulitis scores of 3 week old transgene+ mice was significantly lower, while insulitis scores of 6 week olds were significantly more severe than in wild types controls, Verdaguer J et al, 1997, J. Exp Med 186, 1663-1676.
Transgenic animals bearing both TCR transgenes offer a source of CTL precursors useful in examining the diversity of beta cell peptides recognized by the autoreactive CD8+ T lymphocytes contributing to the earliest phase of IDDM development.

Development
(SJLXC57BL/6J)F2 transgenic mice were backcrossed to NOD. In 2005, the Type 1 Diabetes Resource received congenic NOD hemizygous transgenic mice backcrossed at least 14 generations to NOD.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tcra
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003199   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J
002116   B6.129S2-Tcratm1Mom/J
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
014550   B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
004554   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRaAI4)1Dvs/DvsJ
002115   B6;129S2-Tcratm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
007848   BXSB.129P2(Cg)-Tcratm1Mjo/TheoJ
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002045   C.SJL-Tcrac/SlkJ
002047   C.SJL-Tcrba Tcrac/SlkJ
014639   C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
004444   NOD.129P2(C)-Tcratm1Mjo/DoiJ
006436   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcraAI4)1Dvs/DvsJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/Dvs
004347   NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ
009377   NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
010526   NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ
006303   NOD.FVB-Tg(TcraBDC12-4.1)10Jos/GseJ
004334   NOD/ShiLt-Tg(TcraAI4)1Dvs
018030   SJL.Cg-Tg(TcraTcrbVP2)1Bkim/J
002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcra     (47 strains)

Strains carrying other alleles of Tcrb
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002761   B10.Cg-Tg(TcrAND)53Hed/J
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
003200   B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRB)C14Jg/J
002122   B6.129P2-Tcrbtm1Mom Tcrdtm1Mom/J
002118   B6.129P2-Tcrbtm1Mom/J
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
008684   B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
014550   B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
005655   B6.Cg-Tg(Tcra,Tcrb)3Ayr/J
008428   B6.Cg-Tg(Tcra,Tcrb)HRCAll/J
008429   B6.Cg-Tg(Tcra,Tcrb)HRVAll/J
008006   B6.Cg-Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr/J
004194   B6.Cg-Tg(TcraTcrb)425Cbn/J
005236   B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J
008430   B6.Cg-Tg(Tcrb)HRBAll/J
004555   B6.NOD-(D17Mit21-D17Mit10) Tg(TCRbAI4)1Dvs/DvsJ
002121   B6;129P-Tcrbtm1Mom Tcrdtm1Mom/J
002117   B6;129P2-Tcrbtm1Mom/J
004694   B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ
002408   B6;SJL-Tg(TcrAND)53Hed/J
021880   BXSB.B6-Tg(TcraTcrb)1100Mjb/DcrJ
004364   C.Cg-Tcratm1Mom Tcrbtm1Mom/J
003303   C.Cg-Tg(DO11.10)10Dlo/J
002047   C.SJL-Tcrba Tcrac/SlkJ
002046   C.SJL-Tcrba/SlkJ
011005   C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J
006912   C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J
003831   C57BL/6-Tg(TcraTcrb)1100Mjb/J
003540   C57L/J-Tg(Tcrb)93Vbo/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
007081   CByJ.129P2(B6)-Tcrbtm1Mom/J
005694   D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J
017314   NOD-Tg(TcraTcrb)2H6Lwn/J
023082   NOD.129P2(Cg)-Tcrbtm1Mom/MnkaJ
006437   NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcrbAI4)1Dvs/DvsJ
004257   NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/Dvs
009377   NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004696   NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ
004460   NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
010526   NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ
006304   NOD.FVB-Tg(TcrbBDC12-4.1)82Gse/GseJ
004335   NOD/ShiLt-Tg(TcrbAI4)1Dvs
018030   SJL.Cg-Tg(TcraTcrbVP2)1Bkim/J
002597   STOCK Tg(TcrHEL3A9)1Mmd/J
View Strains carrying other alleles of Tcrb     (49 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Diabetes Mellitus, Insulin-Dependent; IDDM
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(TcraTcrbNY8.3)1Pesa/?

        involves: C57BL/6 * NOD * SJL
  • immune system phenotype
  • pancreas inflammation
    • insulitis at 3 weeks less than in controls   (MGI Ref ID J:44202)
    • insulitis at 6 weeks of age significantly greater than in controls   (MGI Ref ID J:44202)
  • homeostasis/metabolism phenotype
  • increased circulating glucose level
    • diabetes develops in 26% (males) to 33% (females) of mice   (MGI Ref ID J:44202)
    • diabetes slow to develop and moderate   (MGI Ref ID J:44202)
  • endocrine/exocrine gland phenotype
  • pancreas inflammation
    • insulitis at 3 weeks less than in controls   (MGI Ref ID J:44202)
    • insulitis at 6 weeks of age significantly greater than in controls   (MGI Ref ID J:44202)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Rearranged Antigen-Specific T Cell Receptor Transgenes

Research Tools
Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research
      T Cell Receptor Transgenics

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(TcraTcrbNY8.3)1Pesa
Allele Name transgene insertion 1, Pere Santamaria
Allele Type Transgenic (random, expressed)
Common Name(s) 8.3-TCR; 8.3-TCR-TG; 8.3-TCR-alpha/beta transgene; 8.3TCR; NY8.3; Tcr8.3;
Mutation Made By Pere Santamaria,   University of Calgary
Strain of Origin(SJL x C57BL/6)F2
Expressed Gene Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter Tcra, T cell receptor alpha chain, mouse, laboratory
Promoter Tcrb, T cell receptor beta chain, mouse, laboratory
Molecular Note Functional VDJbeta and VJalpha rearrangements were isolated from the beta cell cytotoxic CD8(+) T cell clone NY8.3. Upstream regulatory sequence together with either TCR-beta enhancers or TCR-alpha enhancers, as appropriate, were associated with VDJbetaor VJalpha. [MGI Ref ID J:44202]
 
 

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Verdaguer J; Schmidt D; Amrani A; Anderson B; Averill N; Santamaria P. 1997. Spontaneous autoimmune diabetes in monoclonal T cell nonobese diabetic mice. J Exp Med 186(10):1663-76. [PubMed: 9362527]  [MGI Ref ID J:44202]

Additional References

Tg(TcraTcrbNY8.3)1Pesa related

Amrani A; Serra P; Yamanouchi J; Han B; Thiessen S; Verdaguer J; Santamaria P. 2002. CD154-dependent priming of diabetogenic CD4(+) T cells dissociated from activation of antigen-presenting cells. Immunity 16(5):719-32. [PubMed: 12049723]  [MGI Ref ID J:76802]

Amrani A; Verdaguer J; Anderson B; Utsugi T; Bou S; Santamaria P. 1999. Perforin-independent beta-cell destruction by diabetogenic CD8(+) T lymphocytes in transgenic nonobese diabetic mice. J Clin Invest 103(8):1201-9. [PubMed: 10207172]  [MGI Ref ID J:108737]

Bobbala D; Chen XL; Leblanc C; Mayhue M; Stankova J; Tanaka T; Chen YG; Ilangumaran S; Ramanathan S. 2012. Interleukin-15 plays an essential role in the pathogenesis of autoimmune diabetes in the NOD mouse. Diabetologia 55(11):3010-20. [PubMed: 22890824]  [MGI Ref ID J:191833]

Carrington EM; Kos C; Zhan Y; Krishnamurthy B; Allison J. 2011. Reducing or increasing beta-cell apoptosis without inflammation does not affect diabetes initiation in neonatal NOD mice. Eur J Immunol 41(8):2238-47. [PubMed: 21674480]  [MGI Ref ID J:176813]

Chee J; Angstetra E; Mariana L; Graham KL; Carrington EM; Bluethmann H; Santamaria P; Allison J; Kay TW; Krishnamurthy B; Thomas HE. 2011. TNF receptor 1 deficiency increases regulatory T cell function in nonobese diabetic mice. J Immunol 187(4):1702-12. [PubMed: 21734073]  [MGI Ref ID J:179172]

Chee J; Ko HJ; Skowera A; Jhala G; Catterall T; Graham KL; Sutherland RM; Thomas HE; Lew AM; Peakman M; Kay TW; Krishnamurthy B. 2014. Effector-memory T cells develop in islets and report islet pathology in type 1 diabetes. J Immunol 192(2):572-80. [PubMed: 24337380]  [MGI Ref ID J:207331]

Chong MM; Chen Y; Darwiche R; Dudek NL; Irawaty W; Santamaria P; Allison J; Kay TW; Thomas HE. 2004. Suppressor of cytokine signaling-1 overexpression protects pancreatic beta cells from CD8+ T cell-mediated autoimmune destruction. J Immunol 172(9):5714-21. [PubMed: 15100317]  [MGI Ref ID J:89692]

DiLorenzo TP; Lieberman SM; Takaki T; Honda S; Chapman HD; Santamaria P; Serreze DV; Nathenson SG. 2002. During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. Clin Immunol 105(3):332-41. [PubMed: 12498815]  [MGI Ref ID J:94192]

Dudek NL; Thomas HE; Mariana L; Sutherland RM; Allison J; Estella E; Angstetra E; Trapani JA; Santamaria P; Lew AM; Kay TW. 2006. Cytotoxic T-cells from T-cell receptor transgenic NOD8.3 mice destroy beta-cells via the perforin and Fas pathways. Diabetes 55(9):2412-8. [PubMed: 16936188]  [MGI Ref ID J:116592]

Fallarino F; Grohmann U; You S; McGrath BC; Cavener DR; Vacca C; Orabona C; Bianchi R; Belladonna ML; Volpi C; Santamaria P; Fioretti MC; Puccetti P. 2006. The combined effects of tryptophan starvation and tryptophan catabolites down-regulate T cell receptor zeta-chain and induce a regulatory phenotype in naive T cells. J Immunol 176(11):6752-61. [PubMed: 16709834]  [MGI Ref ID J:131797]

Ghaemi Oskouie F; Shameli A; Yang A; Desrosiers MD; Mucsi AD; Blackburn MR; Yang Y; Santamaria P; Shi Y. 2011. High levels of adenosine deaminase on dendritic cells promote autoreactive T cell activation and diabetes in nonobese diabetic mice. J Immunol 186(12):6798-806. [PubMed: 21593382]  [MGI Ref ID J:175471]

Graham KL; Krishnamurthy B; Fynch S; Mollah ZU; Slattery R; Santamaria P; Kay TW; Thomas HE. 2011. Autoreactive Cytotoxic T Lymphocytes Acquire Higher Expression of Cytotoxic Effector Markers in the Islets of NOD Mice after Priming in Pancreatic Lymph Nodes. Am J Pathol 178(6):2716-25. [PubMed: 21641394]  [MGI Ref ID J:173290]

Graham KL; Sanders N; Tan Y; Allison J; Kay TW; Coulson BS. 2008. Rotavirus infection accelerates type 1 diabetes in mice with established insulitis. J Virol 82(13):6139-49. [PubMed: 18417562]  [MGI Ref ID J:138082]

Han B; Serra P; Yamanouchi J; Amrani A; Elliott JF; Dickie P; Dilorenzo TP; Santamaria P. 2005. Developmental control of CD8 T cell-avidity maturation in autoimmune diabetes. J Clin Invest 115(7):1879-87. [PubMed: 15937548]  [MGI Ref ID J:99646]

Judkowski V; Krakowski M; Rodriguez E; Mocnick L; Santamaria P; Sarvetnick N. 2004. Increased islet antigen presentation leads to type-1 diabetes in mice with autoimmune susceptibility. Eur J Immunol 34(4):1031-40. [PubMed: 15048713]  [MGI Ref ID J:88883]

Krishnamurthy B; Chee J; Jhala G; Fynch S; Graham KL; Santamaria P; Morahan G; Allison J; Izon D; Thomas HE; Kay TW. 2012. Complete diabetes protection despite delayed thymic tolerance in NOD8.3 TCR transgenic mice due to antigen-induced extrathymic deletion of T cells. Diabetes 61(2):425-35. [PubMed: 22190647]  [MGI Ref ID J:191509]

Krishnamurthy B; Dudek NL; McKenzie MD; Purcell AW; Brooks AG; Gellert S; Colman PG; Harrison LC; Lew AM; Thomas HE; Kay TW. 2006. Responses against islet antigens in NOD mice are prevented by tolerance to proinsulin but not IGRP. J Clin Invest 116(12):3258-65. [PubMed: 17143333]  [MGI Ref ID J:117352]

Krishnamurthy B; Mariana L; Gellert SA; Colman PG; Harrison LC; Lew AM; Santamaria P; Thomas HE; Kay TW. 2008. Autoimmunity to Both Proinsulin and IGRP Is Required for Diabetes in Nonobese Diabetic 8.3 TCR Transgenic Mice. J Immunol 180(7):4458-4464. [PubMed: 18354167]  [MGI Ref ID J:132969]

Lee AS; Ghoreishi M; Cheng WK; Chang TY; Zhang YQ; Dutz JP. 2011. Toll-like receptor 7 stimulation promotes autoimmune diabetes in the NOD mouse. Diabetologia :. [PubMed: 21340621]  [MGI Ref ID J:169589]

Leiter EH; Reifsnyder P; Driver J; Kamdar S; Choisy-Rossi C; Serreze DV; Hara M; Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab 9 Suppl 2:14-22. [PubMed: 17919174]  [MGI Ref ID J:127015]

Mohan JF; Calderon B; Anderson MS; Unanue ER. 2013. Pathogenic CD4(+) T cells recognizing an unstable peptide of insulin are directly recruited into islets bypassing local lymph nodes. J Exp Med 210(11):2403-14. [PubMed: 24127484]  [MGI Ref ID J:206540]

Mollah ZU; Graham KL; Krishnamurthy B; Trivedi P; Brodnicki TC; Trapani JA; Kay TW; Thomas HE. 2012. Granzyme B is dispensable in the development of diabetes in non-obese diabetic mice. PLoS One 7(7):e40357. [PubMed: 22792290]  [MGI Ref ID J:189506]

Paterson AM; Brown KE; Keir ME; Vanguri VK; Riella LV; Chandraker A; Sayegh MH; Blazar BR; Freeman GJ; Sharpe AH. 2011. The programmed death-1 ligand 1:B7-1 pathway restrains diabetogenic effector T cells in vivo. J Immunol 187(3):1097-105. [PubMed: 21697456]  [MGI Ref ID J:179115]

Ramanathan S; Dubois S; Chen XL; Leblanc C; Ohashi PS; Ilangumaran S. 2011. Exposure to IL-15 and IL-21 enables autoreactive CD8 T cells to respond to weak antigens and cause disease in a mouse model of autoimmune diabetes. J Immunol 186(9):5131-41. [PubMed: 21430227]  [MGI Ref ID J:172862]

Samanta D; Mukherjee G; Ramagopal UA; Chaparro RJ; Nathenson SG; DiLorenzo TP; Almo SC. 2011. Structural and functional characterization of a single-chain peptide-MHC molecule that modulates both naive and activated CD8+ T cells. Proc Natl Acad Sci U S A 108(33):13682-7. [PubMed: 21825122]  [MGI Ref ID J:175993]

Serra P; Amrani A; Yamanouchi J; Han B; Thiessen S; Utsugi T; Verdaguer J; Santamaria P. 2003. CD40 ligation releases immature dendritic cells from the control of regulatory CD4+CD25+ T cells. Immunity 19(6):877-89. [PubMed: 14670304]  [MGI Ref ID J:86995]

Shameli A; Clemente-Casares X; Wang J; Santamaria P. 2011. Development of memory-like autoregulatory CD8+ T cells is CD4+ T cell dependent. J Immunol 187(6):2859-66. [PubMed: 21824864]  [MGI Ref ID J:179264]

Shameli A; Yamanouchi J; Tsai S; Yang Y; Clemente-Casares X; Moore A; Serra P; Santamaria P. 2013. IL-2 promotes the function of memory-like autoregulatory CD8(+) T cells but suppresses their development via FoxP3(+) Treg cells. Eur J Immunol 43(2):394-403. [PubMed: 23180662]  [MGI Ref ID J:192807]

Soltani N; Qiu H; Aleksic M; Glinka Y; Zhao F; Liu R; Li Y; Zhang N; Chakrabarti R; Ng T; Jin T; Zhang H; Lu WY; Feng ZP; Prud'homme GJ; Wang Q. 2011. GABA exerts protective and regenerative effects on islet beta cells and reverses diabetes. Proc Natl Acad Sci U S A 108(28):11692-7. [PubMed: 21709230]  [MGI Ref ID J:174398]

Thomas HE; Irawaty W; Darwiche R; Brodnicki TC; Santamaria P; Allison J; Kay TW. 2004. IL-1 Receptor Deficiency Slows Progression to Diabetes in the NOD Mouse. Diabetes 53(1):113-121. [PubMed: 14693705]  [MGI Ref ID J:87251]

Tsai S; Shameli A; Yamanouchi J; Clemente-Casares X; Wang J; Serra P; Yang Y; Medarova Z; Moore A; Santamaria P. 2010. Reversal of autoimmunity by boosting memory-like autoregulatory T cells. Immunity 32(4):568-80. [PubMed: 20381385]  [MGI Ref ID J:179859]

Ueno A; Cho S; Cheng L; Wang J; Hou S; Nakano H; Santamaria P; Yang Y. 2007. Transient upregulation of indoleamine 2,3-dioxygenase in dendritic cells by human chorionic gonadotropin downregulates autoimmune diabetes. Diabetes 56(6):1686-93. [PubMed: 17360980]  [MGI Ref ID J:126514]

Verdaguer J; Amrani A; Anderson B; Schmidt D; Santamaria P. 1999. Two mechanisms for the non-MHC-linked resistance to spontaneous autoimmunity. J Immunol 162(8):4614-26. [PubMed: 10202001]  [MGI Ref ID J:109898]

Wang J; Cho S; Ueno A; Cheng L; Xu BY; Desrosiers MD; Shi Y; Yang Y. 2008. Ligand-dependent induction of noninflammatory dendritic cells by anergic invariant NKT cells minimizes autoimmune inflammation. J Immunol 181(4):2438-45. [PubMed: 18684934]  [MGI Ref ID J:140188]

Wang J; Tsai S; Shameli A; Yamanouchi J; Alkemade G; Santamaria P. 2010. In situ recognition of autoantigen as an essential gatekeeper in autoimmune CD8+ T cell inflammation. Proc Natl Acad Sci U S A 107(20):9317-22. [PubMed: 20439719]  [MGI Ref ID J:160286]

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778]  [MGI Ref ID J:120349]

Yi Z; Li L; Garland A; He Q; Wang H; Katz JD; Tisch R; Wang B. 2012. IFN-gamma receptor deficiency prevents diabetes induction by diabetogenic CD4+, but not CD8+, T cells. Eur J Immunol 42(8):2010-8. [PubMed: 22865049]  [MGI Ref ID J:187880]

Ziegler AI; Le Page MA; Maxwell MJ; Stolp J; Guo H; Jayasimhan A; Hibbs ML; Santamaria P; Miller JF; Plebanski M; Silveira PA; Slattery RM. 2013. The CD19 signalling molecule is elevated in NOD mice and controls type 1 diabetes development. Diabetologia 56(12):2659-68. [PubMed: 24013782]  [MGI Ref ID J:203810]

Zwicker KA; Gratton KJ; Santamaria P; Bathe OF. 2003. Tumor immunity in the context of autoimmunity. J Surg Res 114(2):274. [PubMed: 14559559]  [MGI Ref ID J:86058]

de Jersey J; Snelgrove SL; Palmer SE; Teteris SA; Mullbacher A; Miller JF; Slattery RM. 2007. Beta cells cannot directly prime diabetogenic CD8 T cells in nonobese diabetic mice. Proc Natl Acad Sci U S A 104(4):1295-300. [PubMed: 17229843]  [MGI Ref ID J:119514]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryFACS analysis, utlizing Vβ8.1,2,3 FITC antibody costaining with CD8a PE, is used to genotype mice from this strain.

The accelerated diabetes development in NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ hemizygous breeding stock is not significantly retarded by Complete Freund's Adjuvant.

Mating System+/+ sibling x Hemizygote         (Female x Male)   02-MAY-08
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $125.00Female or MaleHemizygous for Tg(TcraTcrbNY8.3)1Pesa  
Price per Pair (US dollars $)Pair Genotype
$195.00Hemizygous for Tg(TcraTcrbNY8.3)1Pesa x Noncarrier  
$195.00Noncarrier x Hemizygous for Tg(TcraTcrbNY8.3)1Pesa  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $162.50Female or MaleHemizygous for Tg(TcraTcrbNY8.3)1Pesa  
Price per Pair (US dollars $)Pair Genotype
$253.50Hemizygous for Tg(TcraTcrbNY8.3)1Pesa x Noncarrier  
$253.50Noncarrier x Hemizygous for Tg(TcraTcrbNY8.3)1Pesa  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

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See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use


General Terms and Conditions


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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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