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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(ACTB-mRFP1)1F1Hadj/J (Changed: 08-MAY-08 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Generation N5F2 (25-JAN-08) Donating Investigator IMR Colony, The Jackson Laboratory Description
Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. Various optical imaging modalities can be used to visualize mRFP1 expressing cells in culture, in embryos and adult mice. Cells from transgenic mRFP1 mice, sampled along with green and cyan fluorescent cells from other mice, show clearly discernable fluorescence. This mouse may be useful in studies of mRFP1 cell lines, transplantation, embryo chimera experiments, and fluorescent imaging and technology development.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and CMV immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was injected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeras were bred to ICR. Founder line 1F1 was obtained and bred to ICR mice for five generations before arrival at The Jackson Laboratory. Upon arrival, this mouse was backcrossed to C57BL/6J mice for at least 5 generations.
Control Information
| Control | ||
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| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-mRFP1)1F1Hadj | ||
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| Allele Name | transgene insertion 1F1, Anna-Katerina Hadjantonakis | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | CAG::mRFP1; Tg(ACTB-mRFP1)1F1Hadj; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. | ||
| Expressed Gene | RFP, Red Fluorescent Protein, jelly fish | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and cytomegalovirus (CMV) immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was injected into(129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. [MGI Ref ID J:104237] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-mRFP1)1F1Hadj, MCA, vers. 2
Tg(CAG-mRFP1)1F1Hadj, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Long JZ; Lackan CS; Hadjantonakis AK. 2005. Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein. BMC Biotechnol 5:20. [PubMed: 15996270] [MGI Ref ID J:104237]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry Upon arrival at The Jackson Laboratory, transgenic mice on a Stock background were bred to C57BL/6J for more than 5 generations. When maintaining a live colony, transgenic positive mice are bred. Mating System +/+ sibling x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $203.80 Female or Male Hemizygous for Tg(CAG-mRFP1)1F1Hadj *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $256.05 Hemizygous for Tg(CAG-mRFP1)1F1Hadj x Noncarrier $256.05 Noncarrier x Hemizygous for Tg(CAG-mRFP1)1F1Hadj
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $265.00 Female or Male Hemizygous for Tg(CAG-mRFP1)1F1Hadj *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $332.90 Hemizygous for Tg(CAG-mRFP1)1F1Hadj x Noncarrier $332.90 Noncarrier x Hemizygous for Tg(CAG-mRFP1)1F1Hadj
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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