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| Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. This mouse may be useful in studies of mRFP1 cell lines, transplantation, embryo chimera experiments, and fluorescent imaging and technology development. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
Strain Information
Former Names B6.Cg-Tg(ACTB-mRFP1)1F1Hadj/J (Changed: 08-MAY-08 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 30-APR-08 Species laboratory mouse Generation N5F16 (21-DEC-10)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. Various optical imaging modalities can be used to visualize mRFP1 expressing cells in culture, in embryos and adult mice. Cells from transgenic mRFP1 mice, sampled along with green and cyan fluorescent cells from other mice, show clearly discernable fluorescence. This mouse may be useful in studies of mRFP1 cell lines, transplantation, embryo chimera experiments, and fluorescent imaging and technology development.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and CMV immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was injected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeras were bred to ICR. Founder line 1F1 was obtained and bred to ICR mice for five generations before arrival at The Jackson Laboratory. Upon arrival, this mouse was backcrossed to C57BL/6J mice for at least 5 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-mRFP1)1F1Hadj | ||
|---|---|---|---|
| Allele Name | transgene insertion 1F1, Anna-Katerina Hadjantonakis | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | CAG::mRFP1; Tg(ACTB-mRFP1)1F1Hadj; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. | ||
| Expressed Gene | RFP, Red Fluorescent Protein, coral | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and cytomegalovirus (CMV) immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was injected into(129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. [MGI Ref ID J:104237] | ||
| Gene Symbol and Name | Tg(CAG-mRFP1)1F1Hadj, transgene insertion 1F1, Anna-Katerina Hadjantonakis | ||
| Chromosome | UN | ||
| Gene Common Name(s) | CAG::mRFP1; Tg(ACTB-mRFP1)1F1Hadj; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-mRFP1)1F1Hadj, Melt Curve Analysis
Tg(CAG-mRFP1)1F1Hadj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Long JZ; Lackan CS; Hadjantonakis AK. 2005. Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein. BMC Biotechnol 5:20. [PubMed: 15996270] [MGI Ref ID J:104237]
Additional References
Tg(CAG-mRFP1)1F1Hadj relatedGrabarek JB; Zyzynska K; Saiz N; Piliszek A; Frankenberg S; Nichols J; Hadjantonakis AK; Plusa B. 2012. Differential plasticity of epiblast and primitive endoderm precursors within the ICM of the early mouse embryo. Development 139(1):129-39. [PubMed: 22096072] [MGI Ref ID J:179000]
Kwon GS; Viotti M; Hadjantonakis AK. 2008. The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages. Dev Cell 15(4):509-20. [PubMed: 18854136] [MGI Ref ID J:143286]
Robbins CS; Chudnovskiy A; Rauch PJ; Figueiredo JL; Iwamoto Y; Gorbatov R; Etzrodt M; Weber GF; Ueno T; van Rooijen N; Mulligan-Kehoe MJ; Libby P; Nahrendorf M; Pittet MJ; Weissleder R; Swirski FK. 2012. Extramedullary hematopoiesis generates Ly-6C(high) monocytes that infiltrate atherosclerotic lesions. Circulation 125(2):364-74. [PubMed: 22144566] [MGI Ref ID J:193795]
Wang G; Guo Q; Hossain M; Fazio V; Zeynalov E; Janigro D; Mayberg MR; Namura S. 2009. Bone marrow-derived cells are the major source of MMP-9 contributing to blood-brain barrier dysfunction and infarct formation after ischemic stroke in mice. Brain Res 1294:183-92. [PubMed: 19646426] [MGI Ref ID J:157206]
Wang X; Chen T; Leng L; Fan J; Cao K; Duan Z; Zhang X; Shao C; Wu M; Tadmori I; Li T; Liang L; Sun D; Zheng S; Meinhardt A; Young W; Bucala R; Ren Y. 2012. MIF produced by bone marrow-derived macrophages contributes to teratoma progression after embryonic stem cell transplantation. Cancer Res 72(11):2867-78. [PubMed: 22461508] [MGI Ref ID J:189342]
Zhang Y; Daquinag A; Traktuev DO; Amaya-Manzanares F; Simmons PJ; March KL; Pasqualini R; Arap W; Kolonin MG. 2009. White adipose tissue cells are recruited by experimental tumors and promote cancer progression in mouse models. Cancer Res 69(12):5259-66. [PubMed: 19491274] [MGI Ref ID J:149658]
Zhang Y; Daquinag AC; Amaya-Manzanares F; Sirin O; Tseng C; Kolonin MG. 2012. Stromal progenitor cells from endogenous adipose tissue contribute to pericytes and adipocytes that populate the tumor microenvironment. Cancer Res 72(20):5198-208. [PubMed: 23071132] [MGI Ref ID J:190900]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry Upon arrival at The Jackson Laboratory, transgenic mice on a Stock background were bred to C57BL/6J for more than 5 generations. When maintaining a live colony, transgenic positive mice are bred. Mating System +/+ sibling x Hemizygote (Female x Male) 30-APR-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(CAG-mRFP1)1F1Hadj
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(CAG-mRFP1)1F1Hadj x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(CAG-mRFP1)1F1Hadj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(CAG-mRFP1)1F1Hadj
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(CAG-mRFP1)1F1Hadj x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(CAG-mRFP1)1F1Hadj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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