Strain Name: |
B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J |
|---|---|
Stock Number: |
005884 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(ACTB-mRFP1)1F1Hadj/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ACTB; RFP; Tg(CAG-mRFP1)1F1Hadj; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N5F2 (25-JAN-08) Strain Description
Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. Various optical imaging modalities can be used to visualize mRFP1 expressing cells in culture, in embryos and adult mice. Cells from transgenic mRFP1 mice, sampled along with green and cyan fluorescent cells from other mice, show clearly discernable fluorescence. This mouse may be useful in studies of mRFP1 cell lines, transplantation, embryo chimera experiments, and fluorescent imaging and technology development.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and CMV immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was injected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeras were bred to ICR. Founder line 1F1 was obtained and bred to ICR mice for five generations before arrival at The Jackson Laboratory. Upon arrival, this mouse was backcrossed to C57BL/6J mice for at least 5 generations.
Gene & Allele Details
| Allele Symbol | Tg(CAG-mRFP1)1F1Hadj | ||
|---|---|---|---|
| Allele Name | transgene insertion 1F1, Anna-Katerina Hadjantonakis | ||
| Common Name(s) | CAG::mRFP1; Tg(ACTB-mRFP1)1F1Hadj; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. | ||
| Expressed Gene | RFP, Red Fluorescent Protein, jelly fish | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The first true monomeric red fluorescent protein (mRFP1) is the result of the stepwise directed evolution of DsRed; introducing 33 amino acid substitutions selecting for improved performance and developmental neutrality. The mRFP1 coding sequence was placed under control of the chicken beta actin promoter and cytomegalovirus (CMV) immediate early enhancer combination, designed to drive high-level constitutive gene expression in ES cells, embryos and adult mice. The construct (CAG-mRFP1) was injected into(129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. [J:104237] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | Upon arrival at The Jackson Laboratory, transgenic mice on a Stock background were bred to C57BL/6J for more than 5 generations. When maintaining a live colony, transgenic positive mice are bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
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005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number FGB29
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
References
Selected Reference(s)
Long JZ; Lackan CS; Hadjantonakis AK. 2005. Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein. BMC Biotechnol 5:20. [PubMed: 15996270] [J:104237]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J |
| Stock Number: | 005884 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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