Strain Name: |
B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J |
|---|---|
Stock Number: |
005895 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | B10.D2 Clone 1 Thy 1.1; B10D2.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J; |
| Genes & Alleles | H2; H2d; Tcra; Tcrb; Thy1; Thy1a; Tg(TcraCl1,TcrbCl1)1Shrm; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Congenic Type JAX® GEMM® Strain - Major Histocompatibility Congenic Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Linda Sherman, The Scripps Research Institute Generation N8+N1F5 (21-DEC-07) Strain Description
Male mice that are hemizygous for the Clone-1 TCR (also called Clone 1 Thy1.1 TCR or Cl.1 TCR) transgene are viable, fertile, and normal in size. Females are very weak and have low fecundity. The donating investigator reports that all transgenic mice are prone to tumor development by 5-6 months of age. The transgene encodes a rearranged low avidity T cell receptor that recognizes an influenza virus hemagglutinin epitope (HA518-526) restricted by MHC class I H-2Kd. Flow cytometric analysis shows appropriate skewing towards the CD8+ T cell compartment in thymocytes and peripheral lymphocytes. Both naive and activated clone 1 T cells exhibit decreased responsiveness when presented with their cognate antigen in vitro and when transferred into mice expressing HA on pancreatic beta cells. CD8+ T cells can be induced to exhibit both effector function and antitumor activity. This mouse is further modified with the Thy1.1 allele, rather than the alternate allele present in C57BL/10, DBA/2, and BALB/c mice. Thus, cell populations derived from these transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. These Clone-1 TCR (also called Clone 1 Thy1.1 TCR or Cl.1 TCR) transgenic mice were designed to optimize conditions for tumor eradication by low avidity tumor-specific T cells and may also be useful in general studies of T cell avidity, tolerance, positive/negative selection, and activation.Of note, Clone-1 TCR mice are also available on a BALB/cByJ congenic background (see Stock No. 005922).
Strain Development
A low avidity influenza hemagglutinin (HA)-specific CD8+ T cell clone (Clone 1) was expanded from the tolerant repertoire of a transgenic mouse expressing HA as a self-antigen on insulin-producing beta cells of the pancreatic islets (InsHA mouse). This T cell receptor sequence, specific for influenza virus HA518-526 epitope restricted by MHC class I H-2Kd, was cloned into Tcra-V1,Tcra-J(J11) and Tcrb-V8.2,Tcrb-D,Tcrb-J(J2.4) expression vectors. The facility generating this mutant used "B10D2/nSnJ" mice derived from Stock No. 000463 as founder stock. The constructs were co-injected into fertilized B10D2/nSnJ eggs. Transgenic mice were bred to B10D2/nSnJ mice for 8 generations. Thereafter, transgenic mice were bred to B10D2/nSnJ mice homozygous for the Thy1a allele (coding for the Thy1.1 leukocyte alloantigen) to generate transgenic mice homozygous for Thy1a. Upon arrival at The Jackson Laboratory, transgenic Thy1a/a males were bred with Stock No. 000463 or wildtype sibling (Thy1.1 positive) females to generate Clone 1 transgene positive Thy1.1 positive mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | H2d | ||
|---|---|---|---|
| Allele Name | d variant | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note | The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB | ||
| Allele Symbol | Tg(TcraCl1,TcrbCl1)1Shrm | ||
| Allele Name | transgene insertion 1, Linda Sherman | ||
| Common Name(s) | Clone-1 TCR; | ||
| Mutation Made By | Linda Sherman, The Scripps Research Institute | ||
| Strain of Origin | B10.D2-Hc1 H2d H2-T18c/nSnJ | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| General Note | CD8+ T cells bearing this transgene, when transferred to mice that develop pancreatic tumors expressing influenza virus HA, can be induced to exhibit both effector function and antitumor activity only if HA-responsive CD4+ T cells are co-transferred and the mice are subsequently infected with influenza virus. : J:97745 | ||
| Molecular Note | The transgene encodes a low avidity T cell receptor (TCR) that recognizes the influenza virus hemagglutinin (HA) epitope HA518-526 in the context of the H2-Kd MHC class I molecule. The rearranged Tcra and Tcrb genes, which have joined Valpha1/Jalpha11 and Vbeta8.2/D/Jbeta2.4, respectively, were amplified from cytotoxic T lymphocyte (CTL) clone 1. Clone 1 was derived from a transgenic B10.D2 mouse that expressed influenza virus HA as a self-antigen in pancreatic beta cells, which resulted in production of lower-avidity TCRs than those made by wild type B10.D2 mice after infection with influenza virus strain A/PR/8/32. [MGI Ref ID J:97745] | ||
| Allele Symbol | Thy1a | ||
| Allele Name | a variant | ||
| Common Name(s) | Thy-1.1; Thy1.1; Thy1a; | ||
| Gene Symbol and Name | Thy1, thymus cell antigen 1, theta | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | CD7; CD90; FLJ33325; T25; Thy 1.2; Thy-1; Thy-1.2; Thy1.1; Thy1.2; | ||
| General Note |
The Thy1 locus determines an antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experimentsdesigned to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900). | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(TcraCl1,TcrbCl1)1Shrm
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, breed female Stock No. 000463 to trangenic positive/Thy1a positive males. Transgenic females are very poor breeders. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying H2d allele
005308 B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 005534 B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ 000462 B10.D2-H2d/n2SnJ 000460 B10.D2-Hc0 H2d H2-T18c/o2SnJ 000461 B10.D2-Hc0 H2d H2-T18c/oSnJ 000463 B10.D2-Hc1 H2d H2-T18c/nSnJ 003147 B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J 000360 B6.C-H2d Mdmg1BALB/cBy/aByJ 000359 B6.C-H2d/bByJ 001893 BRVR.D2-H2d/J 000437 D1.C-H2d H2-T18c/SnJ 003153 WLC.C-H2d.GR-Mtv2/MorJ 003154 WLC.C-H2d/MorJ View Strains carrying H2d (13 strains)
001317 B6.Cg-Igha Thy1a Gpi1a/J 005023 B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J 000406 B6.PL-Thy1a/CyJ 000983 B6.PL/(84NS)CyJ 007687 BKa.Cg-Sox17tm1Sjm Ptprcb Thy1a/J 007686 BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J 005307 CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 005922 CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J 005443 CBy.PL(B6)-Thy1a/ScrJ 005686 NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 004483 NOD.NON-Thy1a/1LtJ 002721 NOD.NON-Thy1a/J 005651 SJL.AK-Thy1a/TseJ 003961 SJL.Cg Thy1a-Noxo1hslt/J
005922 CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 006614 B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J 006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 006554 B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 007027 C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/J 008230 FVB(Cg)-Tg(Thy1-SOD1*G93A)T3Hgrd/J 006143 FVB/N-Tg(Thy1-cre)1Vln/J
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Tcra relatedCancer Research
Tumor Resistance
Immunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes
Research Tools
Cancer Research (anti-tumor activity)
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
H2d relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Thy1a relatedImmunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Immunology and Inflammation Research (T cell specific surface marker)
References
Selected Reference(s)
Additional ReferencesLyman MA; Nugent CT; Marquardt KL; Biggs JA; Pamer EG; Sherman LA. 2005. The fate of low affinity tumor-specific CD8+ T cells in tumor-bearing mice. J Immunol 174(5):2563-72. [PubMed: 15728462] [MGI Ref ID J:97745]
Price and Supply Information
| Strain Name: | B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J |
| Stock Number: | 005895 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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