Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N8+F4 (24-JUL-07)   Donating Investigator Yaacov Barak,   The Jackson Laboratory

Description
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.

Development
A targeting vector was designed to place a single loxP site upstream of exon 4 of the endogenous gene and a loxP-flanked neomycin resistance-thymidine kinase gene cassette downstream of exon 4. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre-recombinase vector. Clones in which the selection cassette was selectively deleted, but the loxP-flanked exon 4 remained intact, were injected into C57BL/6J blastocysts. Chimeric mice were backcrossed to C57BL/6 mice for approximately 8 generations. The resulting heterozygotes were intercrossed to produce homozygotes.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ppard

005828	B6.129P2-Ppardtm1Dgen/J
005901	B6.129S4-Ppardtm2Rev/J

View Strains carrying other alleles of Ppard     (2 strains)

Additional Web Information

Congenic Nomenclature
Cre-lox Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Other

Cardiovascular Research
Other (altered fat metabolism)

Cell Biology Research
Transcriptional Regulation

Diabetes and Obesity Research
Obesity Without Diabetes (diet-induced)

Endocrine Deficiency Research
Adipose Defects

Immunology and Inflammation Research
Inflammation

Internal/Organ Research
Adipose Defects

Metabolism Research
Lipid Metabolism

Research Tools
Cancer Research
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Diabetes and Obesity Research (loxP)
Immunology and Inflammation Research
Reproductive Biology Research (Cre-lox System)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ppardtm1Rev
Allele Name		targeted mutation 1, Ronald M Evans
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Ppardeltack;
Mutation Made By		Ronald Evans,   The Salk Inst for Biological Studies
Gene Symbol and Name		Ppard, peroxisome proliferator activator receptor delta
Chromosome		17
Gene Common Name(s)		FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; PPAR-beta; PPAR-delta; PPARB; PPARdelta/beta; Peroxisome proliferator-activated receptor beta; Pparb/d;
Molecular Note		Exon 4, which encodes the amino terminal portion of the DNA binding domain was left flanked by single loxP sites after a downstream neo cassette was excised via the expression of cre recombinase in vitro. [MGI Ref ID J:73557]

Genotyping

Genotyping Information

Genotyping Protocols

Ppard^tm1Rev, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Barak Y; Liao D; He W; Ong ES; Nelson MC; Olefsky JM; Boland R; Evans RM. 2002. Effects of peroxisome proliferator-activated receptor delta on placentation, adiposity, and colorectal cancer. Proc Natl Acad Sci U S A 99(1):303-8. [PubMed: 11756685]  [MGI Ref ID J:73557]

Additional References

Ppard^tm1Rev related

Cheng L; Ding G; Qin Q; Huang Y; Lewis W; He N; Evans RM; Schneider MD; Brako FA; Xiao Y; Chen YE; Yang Q. 2004. Cardiomyocyte-restricted peroxisome proliferator-activated receptor-delta deletion perturbs myocardial fatty acid oxidation and leads to cardiomyopathy. Nat Med 10(11):1245-50. [PubMed: 15475963]  [MGI Ref ID J:94021]

Kang K; Reilly SM; Karabacak V; Gangl MR; Fitzgerald K; Hatano B; Lee CH. 2008. Adipocyte-derived Th2 cytokines and myeloid PPARdelta regulate macrophage polarization and insulin sensitivity. Cell Metab 7(6):485-95. [PubMed: 18522830]  [MGI Ref ID J:137033]

Muller-Brusselbach S; Komhoff M; Rieck M; Meissner W; Kaddatz K; Adamkiewicz J; Keil B; Klose KJ; Moll R; Burdick AD; Peters JM; Muller R. 2007. Deregulation of tumor angiogenesis and blockade of tumor growth in PPARbeta-deficient mice. EMBO J 26(15):3686-98. [PubMed: 17641685]  [MGI Ref ID J:124107]

Wang YX; Lee CH; Tiep S; Yu RT; Ham J; Kang H; Evans RM. 2003. Peroxisome-proliferator-activated receptor delta activates fat metabolism to prevent obesity. Cell 113(2):159-70. [PubMed: 12705865]  [MGI Ref ID J:83119]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes.
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.