Strain Name:

B6.129S4-Ppardtm1Rev/J

Stock Number:

005897

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation?+F1pN1
Generation Definitions
 
Donating Investigator Yaacov Barak,   University of Pittsburgh

Description
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.

Development
A targeting vector was designed to place a single loxP site upstream of exon 4 of the endogenous gene and a loxP-flanked neomycin resistance-thymidine kinase gene cassette downstream of exon 4. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre-recombinase vector. Clones in which the selection cassette was selectively deleted, but the loxP-flanked exon 4 remained intact, were injected into C57BL/6J blastocysts. Chimeric mice were backcrossed to C57BL/6 mice for approximately 8 generations. The resulting heterozygotes were intercrossed to produce homozygotes.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ppard
005828   B6.129P2-Ppardtm1Dgen/J
005901   B6.129S4-Ppardtm2Rev/J
012383   B6CBA-Tg(Myh6-Ppard)HEDpk/J
View Strains carrying other alleles of Ppard     (3 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Other

Cardiovascular Research
Other
      altered fat metabolism

Cell Biology Research
Transcriptional Regulation

Diabetes and Obesity Research
Obesity Without Diabetes
      diet-induced

Endocrine Deficiency Research
Adipose Defects

Immunology, Inflammation and Autoimmunity Research
Inflammation

Internal/Organ Research
Adipose Defects

Metabolism Research
Lipid Metabolism

Research Tools
Cancer Research
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP
Immunology, Inflammation and Autoimmunity Research
Reproductive Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ppardtm1Rev
Allele Name targeted mutation 1, Ronald M Evans
Allele Type Targeted (Floxed/Frt)
Common Name(s) Ppardeltack;
Mutation Made By Ronald Evans,   The Salk Inst for Biological Studies
Gene Symbol and Name Ppard, peroxisome proliferator activator receptor delta
Chromosome 17
Gene Common Name(s) FAAR; NR1C2; NUC1; NUCI; NUCII; PPAR-delta; PPARB; PPARdelta/beta; Peroxisome proliferator-activated receptor beta; Pparb/d;
Molecular Note Exon 4, which encodes the amino terminal portion of the DNA binding domain was left flanked by single loxP sites after a downstream neo cassette was excised via the expression of cre recombinase in vitro. [MGI Ref ID J:73557]

Genotyping

Genotyping Information

Genotyping Protocols

Ppardtm1Rev, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Barak Y; Liao D; He W; Ong ES; Nelson MC; Olefsky JM; Boland R; Evans RM. 2002. Effects of peroxisome proliferator-activated receptor delta on placentation, adiposity, and colorectal cancer. Proc Natl Acad Sci U S A 99(1):303-8. [PubMed: 11756685]  [MGI Ref ID J:73557]

Additional References

Ppardtm1Rev related

Angione AR; Jiang C; Pan D; Wang YX; Kuang S. 2011. PPARdelta regulates satellite cell proliferation and skeletal muscle regeneration. Skelet Muscle 1(1):33. [PubMed: 22040534]  [MGI Ref ID J:183730]

Cheng L; Ding G; Qin Q; Huang Y; Lewis W; He N; Evans RM; Schneider MD; Brako FA; Xiao Y; Chen YE; Yang Q. 2004. Cardiomyocyte-restricted peroxisome proliferator-activated receptor-delta deletion perturbs myocardial fatty acid oxidation and leads to cardiomyopathy. Nat Med 10(11):1245-50. [PubMed: 15475963]  [MGI Ref ID J:94021]

Degenhardt T; Saramaki A; Malinen M; Rieck M; Vaisanen S; Huotari A; Herzig KH; Muller R; Carlberg C. 2007. Three members of the human pyruvate dehydrogenase kinase gene family are direct targets of the peroxisome proliferator-activated receptor beta/delta. J Mol Biol 372(2):341-55. [PubMed: 17669420]  [MGI Ref ID J:166353]

Ito K; Carracedo A; Weiss D; Arai F; Ala U; Avigan DE; Schafer ZT; Evans RM; Suda T; Lee CH; Pandolfi PP. 2012. A PML-PPAR-delta pathway for fatty acid oxidation regulates hematopoietic stem cell maintenance. Nat Med 18(9):1350-8. [PubMed: 22902876]  [MGI Ref ID J:192771]

Jin Q; Yu LR; Wang L; Zhang Z; Kasper LH; Lee JE; Wang C; Brindle PK; Dent SY; Ge K. 2011. Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation. EMBO J 30(2):249-62. [PubMed: 21131905]  [MGI Ref ID J:168692]

Kang K; Reilly SM; Karabacak V; Gangl MR; Fitzgerald K; Hatano B; Lee CH. 2008. Adipocyte-derived Th2 cytokines and myeloid PPARdelta regulate macrophage polarization and insulin sensitivity. Cell Metab 7(6):485-95. [PubMed: 18522830]  [MGI Ref ID J:137033]

Kocalis HE; Turney MK; Printz RL; Laryea GN; Muglia LJ; Davies SS; Stanwood GD; McGuinness OP; Niswender KD. 2012. Neuron-specific deletion of peroxisome proliferator-activated receptor delta (PPARdelta) in mice leads to increased susceptibility to diet-induced obesity. PLoS One 7(8):e42981. [PubMed: 22916190]  [MGI Ref ID J:190051]

Li Y; Cheng L; Qin Q; Liu J; Lo WK; Brako LA; Yang Q. 2009. High-fat feeding in cardiomyocyte-restricted PPARdelta knockout mice leads to cardiac overexpression of lipid metabolic genes but fails to rescue cardiac phenotypes. J Mol Cell Cardiol 47(4):536-43. [PubMed: 19595695]  [MGI Ref ID J:156561]

Li Y; Yin R; Liu J; Wang P; Wu S; Luo J; Zhelyabovska O; Yang Q. 2009. Peroxisome proliferator-activated receptor delta regulates mitofusin 2 expression in the heart. J Mol Cell Cardiol 46(6):876-82. [PubMed: 19265701]  [MGI Ref ID J:157147]

Monk JM; Kim W; Callaway E; Turk HF; Foreman JE; Peters JM; He W; Weeks B; Alaniz RC; McMurray DN; Chapkin RS. 2012. Immunomodulatory action of dietary fish oil and targeted deletion of intestinal epithelial cell PPARdelta in inflammation-induced colon carcinogenesis. Am J Physiol Gastrointest Liver Physiol 302(1):G153-67. [PubMed: 21940900]  [MGI Ref ID J:183340]

Mukundan L; Odegaard JI; Morel CR; Heredia JE; Mwangi JW; Ricardo-Gonzalez RR; Goh YP; Eagle AR; Dunn SE; Awakuni JU; Nguyen KD; Steinman L; Michie SA; Chawla A. 2009. PPAR-delta senses and orchestrates clearance of apoptotic cells to promote tolerance. Nat Med 15(11):1266-72. [PubMed: 19838202]  [MGI Ref ID J:154294]

Muller-Brusselbach S; Komhoff M; Rieck M; Meissner W; Kaddatz K; Adamkiewicz J; Keil B; Klose KJ; Moll R; Burdick AD; Peters JM; Muller R. 2007. Deregulation of tumor angiogenesis and blockade of tumor growth in PPARbeta-deficient mice. EMBO J 26(15):3686-98. [PubMed: 17641685]  [MGI Ref ID J:124107]

Odegaard JI; Ricardo-Gonzalez RR; Red Eagle A; Vats D; Morel CR; Goforth MH; Subramanian V; Mukundan L; Ferrante AW; Chawla A. 2008. Alternative M2 activation of Kupffer cells by PPARdelta ameliorates obesity-induced insulin resistance. Cell Metab 7(6):496-507. [PubMed: 18522831]  [MGI Ref ID J:137032]

Pan D; Fujimoto M; Lopes A; Wang YX. 2009. Twist-1 is a PPARdelta-inducible, negative-feedback regulator of PGC-1alpha in brown fat metabolism. Cell 137(1):73-86. [PubMed: 19345188]  [MGI Ref ID J:147973]

Scholtysek C; Katzenbeisser J; Fu H; Uderhardt S; Ipseiz N; Stoll C; Zaiss MM; Stock M; Donhauser L; Bohm C; Kleyer A; Hess A; Engelke K; David JP; Djouad F; Tuckermann JP; Desvergne B; Schett G; Kronke G. 2013. PPARbeta/delta governs Wnt signaling and bone turnover. Nat Med 19(5):608-13. [PubMed: 23542786]  [MGI Ref ID J:198360]

Wang YX; Lee CH; Tiep S; Yu RT; Ham J; Kang H; Evans RM. 2003. Peroxisome-proliferator-activated receptor delta activates fat metabolism to prevent obesity. Cell 113(2):159-70. [PubMed: 12705865]  [MGI Ref ID J:83119]

Yin KJ; Deng Z; Hamblin M; Zhang J; Chen YE. 2011. Vascular PPARdelta protects against stroke-induced brain injury. Arterioscler Thromb Vasc Biol 31(3):574-81. [PubMed: 21205987]  [MGI Ref ID J:184175]

Zuo X; Peng Z; Moussalli MJ; Morris JS; Broaddus RR; Fischer SM; Shureiqi I. 2009. Targeted genetic disruption of peroxisome proliferator-activated receptor-delta and colonic tumorigenesis. J Natl Cancer Inst 101(10):762-7. [PubMed: 19436036]  [MGI Ref ID J:149538]

el Azzouzi H; Leptidis S; Dirkx E; Hoeks J; van Bree B; Brand K; McClellan EA; Poels E; Sluimer JC; van den Hoogenhof MM; Armand AS; Yin X; Langley S; Bourajjaj M; Olieslagers S; Krishnan J; Vooijs M; Kurihara H; Stubbs A; Pinto YM; Krek W; Mayr M; da Costa Martins PA; Schrauwen P; De Windt LJ. 2013. The hypoxia-inducible microRNA cluster miR-199a approximately 214 targets myocardial PPARdelta and impairs mitochondrial fatty acid oxidation. Cell Metab 18(3):341-54. [PubMed: 24011070]  [MGI Ref ID J:203819]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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