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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Donating Investigator Yaacov Barak, The Jackson Laboratory Description
Heterozygous mice are viable and fertile. All homozygous mice die in utero. Macrophages homozygous for this mutation have no transcriptional response to very low-density lipoprotein treatment. No evaluation of lacZ expression is published. The donating investigator reports homozygous mice on this background have a similar, albeit earlier, embryonic phenotype as the exon 4 deleted mutants described in other publications (Barak PNAS 2002 99:303-8, Chawla PNAS 2003 100:1268-73, and Lee Science 2003 302:453-7). Heterozygous mice or homozygous embryo-derived cells may be useful in studies of embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.Development
A cell line (clone IIIA4) heterozygous for a loxP-deletion of exon 4 was originally generated from 129S4/SvJae-derived J1 embryonic stem (ES) cells (see Stock No. 005897). A targeting vector designed to replace the wild type exon 4 with a beta-galactosidase gene upstream of the DNA binding domain was electroporated into these cells. Resultant heterozygous cells (lacZ/-) were then injected into C57BL/6J blastocysts. Chimeric mice were backcrossed to C57BL/6 mice for approximately 7 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (176 strains)
005828 B6.129P2-Ppardtm1Dgen/J 005897 B6.129S4-Ppardtm1Rev/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ppardtm2Rev/Ppardtm2Rev
involves: 129S4/SvJae
- lethality-prenatal/perinatal
- prenatal lethality (MGI Ref ID J:105694)
- homozygous embryos die in utero
- embryogenesis phenotype
- abnormal extraembryonic tissue morphology (MGI Ref ID J:105694)
- embryonic phenotype is stated to be similar, albeit earlier, to that of Ppardtm1.1Rev homozygotes; however no data is presented
This mouse can be used to support research in many areas including:
Cancer Research
Other
Cardiovascular Research
Other (altered fat metabolism)
Cell Biology Research
Transcriptional Regulation
Developmental Biology Research
Embryonic Lethality (Homozygous)
Diabetes and Obesity Research
Obesity Without Diabetes (diet-induced)
Endocrine Deficiency Research
Adipose Defects
Immunology and Inflammation Research
Inflammation
Internal/Organ Research
Adipose Defects
Metabolism Research
Lipid Metabolism
Reproductive Biology Research
Research Tools
Cancer Research
Immunology and Inflammation Research
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Ppardtm2Rev | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Ronald M Evans | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Ppardtm1.2Rev; lz; | ||
| Mutation Made By | Ronald Evans, The Salk Inst for Biological Studies | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Ppard, peroxisome proliferator activator receptor delta | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; PPAR-beta; PPAR-delta; PPARB; PPARdelta/beta; Peroxisome proliferator-activated receptor beta; Pparb/d; | ||
| Molecular Note | The wild-type exon 4 is replaced with an in-frame lacZ gene upstream of the DNA binding domain. No evaluation of beta-galactosidase expression is published. [MGI Ref ID J:81838] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ppardtm2Rev, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Barak Y; Liao D; He W; Ong ES; Nelson MC; Olefsky JM; Boland R; Evans RM. 2002. Effects of peroxisome proliferator-activated receptor delta on placentation, adiposity, and colorectal cancer. Proc Natl Acad Sci U S A 99(1):303-8. [PubMed: 11756685] [MGI Ref ID J:73557]
Additional References
Ppardtm2Rev relatedBarak Y. 2006. Creation of the allele Ppard<tm1.2Rev> MGI Direct Data Submission :. [MGI Ref ID J:105694]
Chawla A; Lee CH; Barak Y; He W; Rosenfeld J; Liao D; Han J; Kang H; Evans RM. 2003. PPARdelta is a very low-density lipoprotein sensor in macrophages. Proc Natl Acad Sci U S A 100(3):1268-73. [PubMed: 12540828] [MGI Ref ID J:81838]
Man MQ; Barish GD; Schmuth M; Crumrine D; Barak Y; Chang S; Jiang Y; Evans RM; Elias PM; Feingold KR. 2008. Deficiency of PPARbeta/delta in the epidermis results in defective cutaneous permeability barrier homeostasis and increased inflammation. J Invest Dermatol 128(2):370-7. [PubMed: 17713572] [MGI Ref ID J:133147]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as heterozygotes. All homozygous mice are embryonic lethal.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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