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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Katya Ravid, Boston University School of Medicine Description
Hemizygous and homozygous transgenic mice are viable, fertile, normal in size, and do not display any behavioral abnormalities. Expression of the bicistronic transgene is directed by a tetracycline-responsive regulatory element (TRE; tetO). When transgenic mice are bred with another transgenic strain expressing the tetracycline-controlled transactivator protein (tTA) under the regulation of a tissue-specific promoter, both aurora kinase B (Aurkb) and lacZ cistrons are inducibly expressed in the appropriate tissue in the bitransgenic offspring.This mouse was originally designed to be bred with Tg(Pf4-tTA)42Kra transgenic mice, which express tTA from a megakaryocyte-specific promoter. Megakaryocytes and platelet cells derived from the bitransgenic offspring show inducible and reversible beta-galactosidase expression. Further, megakaryocytes inducibly express Aurkb mRNA (but not protein) with a modest effect on megakaryocyte ploidy and no effect on platelet quantity. These double transgenic mice may be useful in studies of megakaryocyte/platelet lineage cell function, including migration following injury/insult and overexpression of thrombotic agents.
Development
A prokaryotic beta-galactosidase (lacZ) reporter gene under control of the tetracycline-response element (TRE; containing seven direct repeats of the tetracycline operator (tetO) sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter) was placed upstream of a rat Aurora-kinase B cDNA (with N-terminal FLAG-tag sequence and linked to the 3' end of the human beta-globin gene, including the 3' intron and poly A signal). The construct was injected into fertilized FVB/NJ embryos, which were then implanted into pseudopregnant foster mothers. Founder line 41 was obtained and bred to FVB/NJ mice for 3 generations. Transgenic mice were interbred for 5 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (176 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(PF4-tTA/VP16)42Kra/0 Tg(tetO-Aurkb,lacZ)41Kra/0
FVB/NJ-Tg(tetO-Aurkb,lacZ)41Kra Tg(PF4-tTA/VP16)42Kra
- homeostasis/metabolism phenotype
- abnormal platelet physiology (MGI Ref ID J:104239)
- after LPS treatment, mice show accumulation of platelets in the liver and lungs in the induced (doxycycline removed) state
- hematopoietic system phenotype
- abnormal platelet physiology (MGI Ref ID J:104239)
- after LPS treatment, mice show accumulation of platelets in the liver and lungs in the induced (doxycycline removed) state
- abnormal thrombopoiesis (MGI Ref ID J:104239)
- induced mice show no increase in platelet number compared to control
This mouse can be used to support research in many areas including:
lacZ relatedCardiovascular Research
Ischemia studies
Vascular Defects (platelet defect)
Hematological Research
Clotting Defects
Platelet Defects
Research Tools
lacZ Expression
Cardiovascular Research (Tetop Tet System)
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Hematological Research
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-Aurkb,lacZ)41Kra | ||
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| Allele Name | transgene insertion 41, Katya Ravid | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | CMV-Aurora-B/beta-gal; teto-AIM-1; | ||
| Mutation Made By | Katya Ravid, Boston University School of Medicine | ||
| Strain of Origin | FVB/NJ | ||
| Site of Expression | When crossed with a strain that expresses tTA under the regulation of a tissue-specific promoter, the transgene can be inducibly expressed in the target tissue of bitransgenic progeny. | ||
| Expressed Gene | Aurkb, aurora kinase B, rat | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A vector pBI-G (Clontech #6150-1) was used to create this transgene. The vector contains a prokaryotic beta-galactosidase (lacZ) reporter gene under control of the tetracycline-response element (TRE; containing seven direct repeats of the tetracycline operator (tetO) sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter).The rat Aurora-kinase B cDNA (with N-terminal FLAG-tag sequence and 3' linked beta-globin gene) was placed downstream of the promoter and the purified construct was injected into fertilized FVB/NJ eggs to create the transgenic mice. [MGI Ref ID J:104239] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Aurkb,lacZ)41Kra, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Nguyen HG; Yu G; Makitalo M; Yang D; Xie HX; Jones MR; Ravid K. 2005. Conditional overexpression of transgenes in megakaryocytes and platelets in vivo. Blood 106(5):1559-64. [PubMed: 15890684] [MGI Ref ID J:104239]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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