Strain Name: |
FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J |
|---|---|
Stock Number: |
005941 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Strain Common Names | CMV-Aurora-B/beta-gal transgenic mice; |
| Genes & Alleles | Aurkb; lacZ; Tg(tetO-Aurkb,lacZ)41Kra; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Katya Ravid, Boston University School of Medicine Strain Description
Hemizygous and homozygous transgenic mice are viable, fertile, normal in size, and do not display any behavioral abnormalities. Expression of the bicistronic transgene is directed by a tetracycline-responsive regulatory element (TRE; tetO). When transgenic mice are bred with another transgenic strain expressing the tetracycline-controlled transactivator protein (tTA) under the regulation of a tissue-specific promoter, both aurora kinase B (Aurkb) and lacZ cistrons are inducibly expressed in the appropriate tissue in the bitransgenic offspring.This mouse was originally designed to be bred with Tg(Pf4-tTA)42Kra transgenic mice, which express tTA from a megakaryocyte-specific promoter. Megakaryocytes and platelet cells derived from the bitransgenic offspring show inducible and reversible beta-galactosidase expression. Further, megakaryocytes inducibly express Aurkb mRNA (but not protein) with a modest effect on megakaryocyte ploidy and no effect on platelet quantity. These double transgenic mice may be useful in studies of megakaryocyte/platelet lineage cell function, including migration following injury/insult and overexpression of thrombotic agents.
Strain Development
A prokaryotic beta-galactosidase (lacZ) reporter gene under control of the tetracycline-response element (TRE; containing seven direct repeats of the tetracycline operator (tetO) sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter) was placed upstream of a rat Aurora-kinase B cDNA (with N-terminal FLAG-tag sequence and linked to the 3' end of the human beta-globin gene, including the 3' intron and poly A signal). The construct was injected into fertilized FVB/NJ embryos, which were then implanted into pseudopregnant foster mothers. Founder line 41 was obtained and bred to FVB/NJ mice for 3 generations. Transgenic mice were interbred for 5 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(tetO-Aurkb,lacZ)41Kra | ||
|---|---|---|---|
| Allele Name | transgene insertion 41, Katya Ravid | ||
| Common Name(s) | CMV-Aurora-B/beta-gal; teto-AIM-1; | ||
| Mutation Made By | Katya Ravid, Boston University School of Medicine | ||
| Strain of Origin | FVB/NJ | ||
| Site of Expression | When crossed with a strain that expresses tTA under the regulation of a tissue-specific promoter, the transgene can be inducibly expressed in the target tissue of bitransgenic progeny. | ||
| Expressed Gene | Aurkb, aurora kinase B, rat | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A vector pBI-G (Clontech #6150-1) was used to create this transgene. The vector contains a prokaryotic beta-galactosidase (lacZ) reporter gene under control of the tetracycline-response element (TRE; containing seven direct repeats of the tetracycline operator (tetO) sequence fused to a bidirectional cytomegalovirus (CMV) minimal promoter).The rat Aurora-kinase B cDNA (with N-terminal FLAG-tag sequence and 3' linked beta-globin gene) was placed downstream of the promoter and the purified construct was injected into fertilized FVB/NJ eggs to create the transgenic mice. [MGI Ref ID J:104239] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(tetO-Aurkb,lacZ)41Kra
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedCardiovascular Research
Ischemia studies
Vascular Defects (platelet defect)
Hematological Research
Clotting Defects
Platelet Defects
Research Tools
lacZ Expression
Cardiovascular Research (Tetop Tet System)
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Hematological Research
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
lacZ Expression
References
Selected Reference(s)
Nguyen HG; Yu G; Makitalo M; Yang D; Xie HX; Jones MR; Ravid K. 2005. Conditional overexpression of transgenes in megakaryocytes and platelets in vivo. Blood 106(5):1559-64. [PubMed: 15890684] [MGI Ref ID J:104239]
Price and Supply Information
| Strain Name: | FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J |
| Stock Number: | 005941 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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