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Strain Name:

FVB/N-Tg(Pf4-tTA/VP16)42Kra/J

Stock Number:

005942

Availability:

Repository-Cryopreserved

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Genes & Alleles   Pf4;   tTA;   Tg(PF4-tTA/VP16)42Kra;

Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Katya Ravid,   Boston University School of Medicine
GenerationN7p

Strain Description
Transgenic mice are viable, fertile, normal in size, and do not display any behavioral abnormalities. Transgenic tetracycline-controlled transactivator protein (tTA) expression is limited to megakaryocytes by the rat chemokine (C-X-C motif) ligand 4 (Cxcl4; formerly called platelet factor four (Pf4)) promoter. When bred to mice carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene is induced specifically in megakaryocytes. Administration of tetracycline/doxycycline sequesters the transgenic protein, and prevents expression of the TRE-regulated target gene.

This mouse originally was designed to be bred with Tg(tetO-Aurkb,lacZ)41Kra (see Stock No. 005941); a transgenic mouse with a bidirectional tetO promoter controlling a mitotic regulator gene (aurora kinase B) and a beta-galactosidase reporter. Megakaryocytes and platelet cells from the bitransgenic offspring show inducible and reversible beta-galactosidase expression. Further, megakaryocytes inducibly express Aurora kinase-B mRNA (but not protein) with a modest effect on megakaryocyte ploidy and no effect on platelet quantity. These mice may be useful in studies of megakaryocyte/platelet lineage cell function, including migration following injury/insult and overexpression of thrombotic agents.

Strain Development
A transgenic construct was designed using the promoter region from rat chemokine (C-X-C motif) ligand 4 (Cxcl4; formerly called platelet factor four (Pf4)) to direct expression of the tetracycline-regulated transactivator, tTA. The construct was injected into fertilized FVB/NJ eggs which were subsequently implanted into pseudopregnant foster mothers. Founder line 42 was obtained and bred to FVB/NJ mice for 3 generations. Transgenic mice were interbred for 5 generations.

Mammalian Phenotype Terms assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Tg(PF4-tTA/VP16)42Kra/0 Tg(tetO-Aurkb,lacZ)41Kra/0

        FVB/NJ-Tg(tetO-Aurkb,lacZ)41Kra Tg(PF4-tTA/VP16)42Kra
  • homeostasis/metabolism phenotype
  • abnormal platelet physiology (MGI Ref ID J:104239)
    • after LPS treatment, mice show accumulation of platelets in the liver and lungs in the induced (doxycycline removed) state
  • hematopoietic system phenotype
  • abnormal platelet physiology (MGI Ref ID J:104239)
    • after LPS treatment, mice show accumulation of platelets in the liver and lungs in the induced (doxycycline removed) state
  • abnormal thrombopoiesis (MGI Ref ID J:104239)
    • induced mice show no increase in platelet number compared to control

Gene & Allele Details

Allele Symbol Tg(PF4-tTA/VP16)42Kra
Allele Name transgene insertion 42, Katya Ravid
Common Name(s) PF4-tTA-VP16;
Strain of OriginFVB/NJ
ES Cell Line Strain129
Site of ExpressionExpresses tTA in megakaryocytes.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter Pf4, platelet factor 4, rat
Molecular Note The rat platelet factor 4 (RP4) promoter was cloned into the tTA-containing plasmid, replacing the existing CMV promoter. The promoter will drive expression of the transactivator tTA fused to the viral coactivator VP16. Expression is repressed with administration of doxycycline and is induced by removal of doxycycline from the diet of the mice.The transgene also contains 1.7 kb of the 3'end of the human beta-globin gene including the 3' intron and polyadenylation signal to permit stable RNA production inmice. A stop codon in the transgene prevents translation of beta-globin into the protein. [MGI Ref ID J:104239]

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of tTA     (19 strains)

Additional Web Information

Tet Expression Systems

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Tet Expression Systems (tTA/rtTA Expressing Strains)

References

Selected Reference(s)

Nguyen HG; Yu G; Makitalo M; Yang D; Xie HX; Jones MR; Ravid K. 2005. Conditional overexpression of transgenes in megakaryocytes and platelets in vivo. Blood 106(5):1559-64. [PubMed: 15890684]  [MGI Ref ID J:104239]

Additional References

Price and Supply Information

Strain Name: FVB/N-Tg(Pf4-tTA/VP16)42Kra/J
Stock Number: 005942

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

General Terms and Conditions

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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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