Description

Strain Information

Former Names B6.129S1-Csf2rb2tm1Cgb Csf2rb1tm1Clsc/J    (Changed: 26-JUN-07 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation ?+F1p   Donating Investigator Lorraine Robb,   The Walter and ElizaHall Institute

Description
Mice homozygous at both loci are viable and fertile with no behavioral abnormalities. Steady state hematopoiesis is normal. Bone marrow from homozygous mice fail to exhibit any cellular responses to GM-CSF, IL-5, or IL-3. Like the similar strain (Stock No. 005940; Csf2rb^tm1Cgb), double homozygous mice exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis (PAP), infection resistance, and attenuated tissue repair after injury. This mutant may be useful in studies of PAP, lung physiology and disease, surfactant homeostasis and metabolism, innate immunity, cytokine signaling, myoproliferative disease, hematopoietic cell populations requiring IL-3, GM-CSF, or IL-5 for development and function, and models of ischemic, traumatic, toxic, and inflammatory injuries.

Development
A targeting vector designed to replace exons 9-13 of Csf2rb2 (β-IL3 or BIL3) with a neomycin resistance cassette was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. The ES cells determined to be heterozygous for this mutation were subsequently electroporated with another targeting vector designed to insert a hygromycin-resistance gene into exon 7 of Csf2rb (βc or Bc). Correctly targeted ES cells (containing both targeted mutations) were injected into C57 blastocysts and the resulting chimeric mice were bred with C57BL/6. Mutant mice were bred to C57BL/6 for 10 generations and then intercrossed to produce a mouse line homozygous for both mutant loci before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Csf2rb

005940

B6.129S1-Csf2rbtm1Cgb/J

View Strains carrying other alleles of Csf2rb     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Ischemia studies
Vascular Defects (defective macrophage function)

Hematological Research
Hematopoietic Defects
Immunological Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines

Internal/Organ Research
Lung Defects
Wound Healing

Research Tools
Immunology and Inflammation Research (Macrophage Deficiency)
Toxicology Research (free radical research)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Csf2rb2tm1Cgb
Allele Name		targeted mutation 1, C Glenn Begley
Allele Type		Targeted (knock-out)
Common Name(s)		BIL3-; Betail3-;
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		W9.5/W95
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Csf2rb2, colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Chromosome		15
Gene Common Name(s)		AIC2A; BetaIl3; Bil3; Csfgmrb; Il3r; Il3rb; Il3rb2; colony stimulating factor, granulocyte macrophage receptor, beta chain; interleukin 3 receptor beta chain; interleukin 3 receptor, beta chain 2;
Molecular Note		Exons 9-13 were replaced with a neomycin resistance cassette. [MGI Ref ID J:32978]
Allele Symbol		Csf2rbtm1Clsc
Allele Name		targeted mutation 1, Clare L Scott
Allele Type		Targeted (knock-out)
Common Name(s)		betac/betaIL3 null;
Gene Symbol and Name		Csf2rb, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Chromosome		15
Gene Common Name(s)		AI848964; AIC2B; Bc; CD131; CDw131; Csf2rb1; Csfgmrb; IL3RB; IL5RB; Il3r; Il3rb; Il3rb1; Il5rb; beta c; colony stimulating factor 2 receptor, beta 1, low-affinity (granulocyte-macrophage); colony stimulating factor, granulocyte macrophage receptor, beta chain; common beta chain; expressed sequence AI848964; interleukin 3 receptor beta chain; interleukin 3 receptor, beta chain 1; interleukin 5 receptor beta;
Molecular Note		ES cells already containing the Csf2rb2tm2Cgb mutation were targeted a second time with a vector containing a hygromycin resistance-gene in exon 7 and a TK cassette at the upstream end of the construct. Three different ES cell lines were used to create chimeric mice. [MGI Ref ID J:109889]

Genotyping

Genotyping Information

Genotyping Protocols

Csf2rb2^tm1Cgb, SEP PCR, vers. 1
Csf2rb^tm1Clsc, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Nicola NA; Robb L; Metcalf D; Cary D; Drinkwater CC; Begley CG. 1996. Functional inactivation in mice of the gene for the interleukin-3 (IL-3)-specific receptor beta-chain: implications for IL-3 function and the mechanism of receptor transmodulation in hematopoietic cells. Blood 87(7):2665-74. [PubMed: 8639882]  [MGI Ref ID J:32978]

Robb L; Drinkwater CC; Metcalf D; Li R; Kontgen F; Nicola NA; Begley CG. 1995. Hematopoietic and lung abnormalities in mice with a null mutation of the common beta subunit of the receptors for granulocyte-macrophage colony-stimulating factor and interleukins 3 and 5. Proc Natl Acad Sci U S A 92(21):9565-9. [PubMed: 7568173]  [MGI Ref ID J:29737]

Scott CL; Robb L; Papaevangeliou B; Mansfield R; Nicola NA; Begley CG. 2000. Reassessment of interactions between hematopoietic receptors using common beta-chain and interleukin-3-specific receptor beta-chain-null cells: no evidence of functional interactions with receptors for erythropoietin, granulocyte colony-stimulating factor, or stem cell factor. Blood 96(4):1588-90. [PubMed: 10942411]  [MGI Ref ID J:109889]

Additional References

Csf2rb2^tm1Cgb related

Asquith KL; Ramshaw HS; Hansbro PM; Beagley KW; Lopez AF; Foster PS. 2008. The IL-3/IL-5/GM-CSF common receptor plays a pivotal role in the regulation of Th2 immunity and allergic airway inflammation. J Immunol 180(2):1199-206. [PubMed: 18178860]  [MGI Ref ID J:130939]

Csf2rb^tm1Clsc related

Asquith KL; Ramshaw HS; Hansbro PM; Beagley KW; Lopez AF; Foster PS. 2008. The IL-3/IL-5/GM-CSF common receptor plays a pivotal role in the regulation of Th2 immunity and allergic airway inflammation. J Immunol 180(2):1199-206. [PubMed: 18178860]  [MGI Ref ID J:130939]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred homozygous at both loci.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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