Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S1-Csf2rb2tm1Cgb Csf2rb1tm1Clsc/J    (Changed: 26-JUN-07 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Lorraine Robb,   The Walter and ElizaHall Institute

Description
Mice homozygous at both loci are viable and fertile with no behavioral abnormalities. Steady state hematopoiesis is normal. Bone marrow from homozygous mice fail to exhibit any cellular responses to GM-CSF, IL-5, or IL-3. Like the similar strain (Stock No. 005940; Csf2rb^tm1Cgb), double homozygous mice exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis (PAP), infection resistance, and attenuated tissue repair after injury. This mutant may be useful in studies of PAP, lung physiology and disease, surfactant homeostasis and metabolism, innate immunity, cytokine signaling, myoproliferative disease, hematopoietic cell populations requiring IL-3, GM-CSF, or IL-5 for development and function, and models of ischemic, traumatic, toxic, and inflammatory injuries.

Development
A targeting vector designed to replace exons 9-13 of Csf2rb2 (β-IL3 or BIL3) with a neomycin resistance cassette was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. The ES cells determined to be heterozygous for this mutation were subsequently electroporated with another targeting vector designed to insert a hygromycin-resistance gene into exon 7 of Csf2rb (βc or Bc). Correctly targeted ES cells (containing both targeted mutations) were injected into C57 blastocysts and the resulting chimeric mice were bred with C57BL/6. Mutant mice were bred to C57BL/6 for 10 generations and then intercrossed to produce a mouse line homozygous for both mutant loci before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Csf2rb

005940

B6.129S1-Csf2rbtm1Cgb/J

View Strains carrying other alleles of Csf2rb     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Surfactant Metabolism Dysfunction, Pulmonary, 5; SMDP5   (CSF2RB)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Csf2rb^tm1Clsc/Csf2rb^tm1Clsc Csf2rb2^tm1Cgb/Csf2rb2^tm1Cgb

        C.129S1-Csf2rb2^tm1Cgb Csf2rb^tm1Clsc

• immune system phenotype
• abnormal T cell physiology
  • less CD4⁺ and CD8⁺ T cells are recruited to the lung after allergy challenge than controls   (MGI Ref ID J:130939)
• • abnormal T cell activation
    • less peribronchial lymph node T cells are in an activated state after allergy challenge compared to controls   (MGI Ref ID J:130939)
    • less activated CD4⁺ T cells are found in the lung after allergy challenge than in controls   (MGI Ref ID J:130939)
  • • decreased T cell proliferation
      • T cells from allergic mice proliferate less vigorously in response to allergic antigen than controls   (MGI Ref ID J:130939)
• abnormal dendritic cell physiology
  • dendritic cells have impaired migration to the lung of both naïve and allergy challenged mice compared to respective controls   (MGI Ref ID J:130939)
  • myeloid dendritic cells in the lung fail to become activated in response to intranasal allergy challenge   (MGI Ref ID J:130939)
• • abnormal plasmacytoid dendritic cell physiology
    • plasmacytoid dendritic cells have impaired migration to the lung of both naïve and allergy challenged mice compared to respective controls   (MGI Ref ID J:130939)
• abnormal interleukin secretion   (MGI Ref ID J:130939)
• • decreased interleukin-13 secretion
    • peribronchial lymph node T cells isolated from allergic mice produce much less IL-13 than controls upon reencounter with allergic antigen   (MGI Ref ID J:130939)
  • decreased interleukin-4 secretion
    • peribronchial lymph node T cells isolated from allergic mice produce much less IL-4 than controls upon reencounter with allergic antigen   (MGI Ref ID J:130939)
  • decreased interleukin-5 secretion
    • peribronchial lymph node T cells isolated from allergic mice produce much less IL-5 than controls upon reencounter with allergic antigen   (MGI Ref ID J:130939)
• decreased susceptibility to type I hypersensitivity reaction
  • mice fail to increase airway resistance in response to intranasal challenge with an antigen mice were previously sensitized too   (MGI Ref ID J:130939)
  • the number of mucus-secreting cells in the lung is half that of wild-type controls after intranasal antigen challenge   (MGI Ref ID J:130939)
  • 10-fold less antigen-specific IgE is detected in the sera of mice after antigen challenge   (MGI Ref ID J:130939)
• impaired eosinophil recruitment
  • eosinophils fail to migrate to the blood, lung or bronchoalveolar fluid after intranasal challenge with an antigen mice were previously sensitized to   (MGI Ref ID J:130939)
  • eosinophil numbers in the peripheral blood, airway lumen, and lung tissue remained at baseline levels for the entire observational period of 2 wk after the final antigen challenge   (MGI Ref ID J:130939)
• increased IgG1 level
  • sera levels of antigen-specific IgG1 are elevated relative to controls in response to challenge with an allergic antigen   (MGI Ref ID J:130939)
• lymph node hyperplasia
  • peribronchial lymph nodes contain about twice as more B cells, CD4⁺ T cells, and CD8⁺ T cells than in controls   (MGI Ref ID J:130939)
  • peribronchial lymph nodes also contain more myeloid and plasmacytoid dendritic cells   (MGI Ref ID J:130939)
• respiratory system phenotype
• decreased airway responsiveness
  • mice fail to increase airway resistance in response to intranasal antigen challenge   (MGI Ref ID J:130939)
• hematopoietic system phenotype
• abnormal T cell activation
  • less peribronchial lymph node T cells are in an activated state after allergy challenge compared to controls   (MGI Ref ID J:130939)
  • less activated CD4⁺ T cells are found in the lung after allergy challenge than in controls   (MGI Ref ID J:130939)
• • decreased T cell proliferation
    • T cells from allergic mice proliferate less vigorously in response to allergic antigen than controls   (MGI Ref ID J:130939)
• cellular phenotype
• abnormal T cell activation
  • less peribronchial lymph node T cells are in an activated state after allergy challenge compared to controls   (MGI Ref ID J:130939)
  • less activated CD4⁺ T cells are found in the lung after allergy challenge than in controls   (MGI Ref ID J:130939)
• • decreased T cell proliferation
    • T cells from allergic mice proliferate less vigorously in response to allergic antigen than controls   (MGI Ref ID J:130939)

Csf2rb^tm1Clsc/Csf2rb^tm1Clsc Csf2rb2^tm1Cgb/Csf2rb2^tm1Cgb

        involves: 129S1/Sv

• hematopoietic system phenotype
• abnormal bone marrow cell number
  • bone marrow cells from mice 6-14 weeks of age do not proliferate when exposed to cytokines like Il-3 or GM-CSF but response to other cytokines is normal   (MGI Ref ID J:109889)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Ischemia studies
Vascular Defects
      defective macrophage function

Hematological Research
Hematopoietic Defects
Immunological Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Internal/Organ Research
Lung Defects
Wound Healing

Research Tools
Immunology and Inflammation Research
      Macrophage Deficiency
Toxicology Research
      free radical research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Csf2rb2tm1Cgb
Allele Name		targeted mutation 1, C Glenn Begley
Allele Type		Targeted (knock-out)
Common Name(s)		BIL3-; Betail3-;
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		W9.5/W95
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Csf2rb2, colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Chromosome		15
Gene Common Name(s)		AIC2A; BetaIl3; Bil3; CD131; CDw131; Csf2rb1; Csfgmrb; IL3RB; IL5RB; Il3r; Il3rb; Il3rb2; SMDP5; colony stimulating factor, granulocyte macrophage receptor, beta chain; interleukin 3 receptor beta chain; interleukin 3 receptor, beta chain 2;
Molecular Note		Exons 9-13 were replaced with a neomycin resistance cassette. [MGI Ref ID J:32978]
Allele Symbol		Csf2rbtm1Clsc
Allele Name		targeted mutation 1, Clare L Scott
Allele Type		Targeted (knock-out)
Common Name(s)		betac null;
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		W9.5/W95
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Csf2rb, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Chromosome		15
Gene Common Name(s)		AI848964; AIC2B; Bc; CD131; CDw131; Csf2rb1; Csfgmrb; IL3RB; IL5RB; Il3r; Il3rb; Il3rb1; Il5rb; SMDP5; beta c; colony stimulating factor 2 receptor, beta 1, low-affinity (granulocyte-macrophage); colony stimulating factor, granulocyte macrophage receptor, beta chain; common beta chain; expressed sequence AI848964; interleukin 3 receptor beta chain; interleukin 3 receptor, beta chain 1; interleukin 5 receptor beta;
General Note		This allele was created on a chromosome already carrying the Csf2rb2tm2Cgb allele. Mice homozygous for both alleles are referred to as betac/betaIL3 null mice.
Molecular Note		ES cells already containing the Csf2rb2tm2Cgb mutation were targeted a second time with a vector containing a hygromycin resistance-gene in exon 7 and a TK cassette at the upstream end of the construct. Three different ES cell lines were used to create chimeric mice. [MGI Ref ID J:109889]

Genotyping

Genotyping Information

Genotyping Protocols

Csf2rb2^tm1Cgb, Separated PCR
Csf2rb^tm1Clsc, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Nicola NA; Robb L; Metcalf D; Cary D; Drinkwater CC; Begley CG. 1996. Functional inactivation in mice of the gene for the interleukin-3 (IL-3)-specific receptor beta-chain: implications for IL-3 function and the mechanism of receptor transmodulation in hematopoietic cells. Blood 87(7):2665-74. [PubMed: 8639882]  [MGI Ref ID J:32978]

Robb L; Drinkwater CC; Metcalf D; Li R; Kontgen F; Nicola NA; Begley CG. 1995. Hematopoietic and lung abnormalities in mice with a null mutation of the common beta subunit of the receptors for granulocyte-macrophage colony-stimulating factor and interleukins 3 and 5. Proc Natl Acad Sci U S A 92(21):9565-9. [PubMed: 7568173]  [MGI Ref ID J:29737]

Scott CL; Robb L; Papaevangeliou B; Mansfield R; Nicola NA; Begley CG. 2000. Reassessment of interactions between hematopoietic receptors using common beta-chain and interleukin-3-specific receptor beta-chain-null cells: no evidence of functional interactions with receptors for erythropoietin, granulocyte colony-stimulating factor, or stem cell factor. Blood 96(4):1588-90. [PubMed: 10942411]  [MGI Ref ID J:109889]

Additional References

Csf2rb2^tm1Cgb related

Asquith KL; Ramshaw HS; Hansbro PM; Beagley KW; Lopez AF; Foster PS. 2008. The IL-3/IL-5/GM-CSF common receptor plays a pivotal role in the regulation of Th2 immunity and allergic airway inflammation. J Immunol 180(2):1199-206. [PubMed: 18178860]  [MGI Ref ID J:130939]

Gautier EL; Chow A; Spanbroek R; Marcelin G; Greter M; Jakubzick C; Bogunovic M; Leboeuf M; van Rooijen N; Habenicht AJ; Merad M; Randolph GJ. 2012. Systemic analysis of PPARgamma in mouse macrophage populations reveals marked diversity in expression with critical roles in resolution of inflammation and airway immunity. J Immunol 189(5):2614-24. [PubMed: 22855714]  [MGI Ref ID J:189849]

Giacomin PR; Siracusa MC; Walsh KP; Grencis RK; Kubo M; Comeau MR; Artis D. 2012. Thymic stromal lymphopoietin-dependent basophils promote Th2 cytokine responses following intestinal helminth infection. J Immunol 189(9):4371-8. [PubMed: 23024277]  [MGI Ref ID J:190604]

Greter M; Helft J; Chow A; Hashimoto D; Mortha A; Agudo-Cantero J; Bogunovic M; Gautier EL; Miller J; Leboeuf M; Lu G; Aloman C; Brown BD; Pollard JW; Xiong H; Randolph GJ; Chipuk JE; Frenette PS; Merad M. 2012. GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells. Immunity 36(6):1031-46. [PubMed: 22749353]  [MGI Ref ID J:187407]

Kim S; Prout M; Ramshaw H; Lopez AF; LeGros G; Min B. 2010. Cutting edge: basophils are transiently recruited into the draining lymph nodes during helminth infection via IL-3, but infection-induced Th2 immunity can develop without basophil lymph node recruitment or IL-3. J Immunol 184(3):1143-7. [PubMed: 20038645]  [MGI Ref ID J:159532]

Lesokhin AM; Hohl TM; Kitano S; Cortez C; Hirschhorn-Cymerman D; Avogadri F; Rizzuto GA; Lazarus JJ; Pamer EG; Houghton AN; Merghoub T; Wolchok JD. 2012. Monocytic CCR2+ Myeloid-Derived Suppressor Cells Promote Immune Escape by Limiting Activated CD8 T-cell Infiltration into the Tumor Microenvironment. Cancer Res 72(4):876-86. [PubMed: 22174368]  [MGI Ref ID J:181100]

Siracusa MC; Saenz SA; Hill DA; Kim BS; Headley MB; Doering TA; Wherry EJ; Jessup HK; Siegel LA; Kambayashi T; Dudek EC; Kubo M; Cianferoni A; Spergel JM; Ziegler SF; Comeau MR; Artis D. 2011. TSLP promotes interleukin-3-independent basophil haematopoiesis and type 2 inflammation. Nature 477(7363):229-33. [PubMed: 21841801]  [MGI Ref ID J:176058]

Skold M; Behar SM. 2008. Tuberculosis triggers a tissue-dependent program of differentiation and acquisition of effector functions by circulating monocytes. J Immunol 181(9):6349-60. [PubMed: 18941226]  [MGI Ref ID J:140726]

Csf2rb^tm1Clsc related

Asquith KL; Ramshaw HS; Hansbro PM; Beagley KW; Lopez AF; Foster PS. 2008. The IL-3/IL-5/GM-CSF common receptor plays a pivotal role in the regulation of Th2 immunity and allergic airway inflammation. J Immunol 180(2):1199-206. [PubMed: 18178860]  [MGI Ref ID J:130939]

Edelson BT; Bradstreet TR; Kc W; Hildner K; Herzog JW; Sim J; Russell JH; Murphy TL; Unanue ER; Murphy KM. 2011. Batf3-Dependent CD11b Peripheral Dendritic Cells Are GM-CSF-Independent and Are Not Required for Th Cell Priming after Subcutaneous Immunization. PLoS One 6(10):e25660. [PubMed: 22065991]  [MGI Ref ID J:178066]

Giacomin PR; Siracusa MC; Walsh KP; Grencis RK; Kubo M; Comeau MR; Artis D. 2012. Thymic stromal lymphopoietin-dependent basophils promote Th2 cytokine responses following intestinal helminth infection. J Immunol 189(9):4371-8. [PubMed: 23024277]  [MGI Ref ID J:190604]

Greter M; Helft J; Chow A; Hashimoto D; Mortha A; Agudo-Cantero J; Bogunovic M; Gautier EL; Miller J; Leboeuf M; Lu G; Aloman C; Brown BD; Pollard JW; Xiong H; Randolph GJ; Chipuk JE; Frenette PS; Merad M. 2012. GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells. Immunity 36(6):1031-46. [PubMed: 22749353]  [MGI Ref ID J:187407]

Kim S; Prout M; Ramshaw H; Lopez AF; LeGros G; Min B. 2010. Cutting edge: basophils are transiently recruited into the draining lymph nodes during helminth infection via IL-3, but infection-induced Th2 immunity can develop without basophil lymph node recruitment or IL-3. J Immunol 184(3):1143-7. [PubMed: 20038645]  [MGI Ref ID J:159532]

Lesokhin AM; Hohl TM; Kitano S; Cortez C; Hirschhorn-Cymerman D; Avogadri F; Rizzuto GA; Lazarus JJ; Pamer EG; Houghton AN; Merghoub T; Wolchok JD. 2012. Monocytic CCR2+ Myeloid-Derived Suppressor Cells Promote Immune Escape by Limiting Activated CD8 T-cell Infiltration into the Tumor Microenvironment. Cancer Res 72(4):876-86. [PubMed: 22174368]  [MGI Ref ID J:181100]

Schneider E; Petit-Bertron AF; Bricard R; Levasseur M; Ramadan A; Girard JP; Herbelin A; Dy M. 2009. IL-33 activates unprimed murine basophils directly in vitro and induces their in vivo expansion indirectly by promoting hematopoietic growth factor production. J Immunol 183(6):3591-7. [PubMed: 19684081]  [MGI Ref ID J:152329]

Siracusa MC; Saenz SA; Hill DA; Kim BS; Headley MB; Doering TA; Wherry EJ; Jessup HK; Siegel LA; Kambayashi T; Dudek EC; Kubo M; Cianferoni A; Spergel JM; Ziegler SF; Comeau MR; Artis D. 2011. TSLP promotes interleukin-3-independent basophil haematopoiesis and type 2 inflammation. Nature 477(7363):229-33. [PubMed: 21841801]  [MGI Ref ID J:176058]

Zhan Y; Carrington EM; van Nieuwenhuijze A; Bedoui S; Seah S; Xu Y; Wang N; Mintern JD; Villadangos JA; Wicks IP; Lew AM. 2011. GM-CSF increases cross-presentation and CD103 expression by mouse CD8(+) spleen dendritic cells. Eur J Immunol 41(9):2585-95. [PubMed: 21660938]  [MGI Ref ID J:176817]

Zhan Y; Vega-Ramos J; Carrington EM; Villadangos JA; Lew AM; Xu Y. 2012. The inflammatory cytokine, GM-CSF, alters the developmental outcome of murine dendritic cells. Eur J Immunol 42(11):2889-900. [PubMed: 22806691]  [MGI Ref ID J:188716]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred homozygous at both loci.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.