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Strain Name:

B6.Cg-Tg(GFAP-tTA)110Pop/J

Stock Number:

005964

Availability:

Repository- Live

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Additional Licensing and Use Restrictions

Genes & Alleles   GFAP;   tTA;   Tg(GFAP-tTA)110Pop;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHemizygote x +/+ sibling         (Female x Male)
Specieslaboratory mouse
Donating Investigator Brian Popko,   University of Chicago
Generation?+N1F3 (24-JUL-07)

Strain Description
Mice hemizygous for the transgene are viable fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express a tetracycline-controlled transactivator protein (tTA) driven by the human glial fibrillary acidic protein (GFAP) promoter. When these mice are mated to a second transgenic strain that carries a target gene under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in astrocytes in the bitransgenic offspring can be induced by withdrawal of the tetracycline analog, doxycycline. Doxycycline may be administered in the animals' water supply. This strain represents an effective tool for generating bitrangenic animals that may be useful to study inducible gene expression in the astrocytes of the central nervous system.

Strain Development
A transgenic construct in which tetracycline-responsive transcriptional activator (tTA) is under the control of the human glial fibrillary acidic protein GFAP promoter was injected into fertilized B6D2F1 mouse eggs. The resulting founder line 110 was backcrossed onto the C57BL/6J background for more than 10 generations.

Gene & Allele Details

Allele Symbol Tg(GFAP-tTA)110Pop
Allele Name transgene insertion 110, Brian Popko
Common Name(s) GFAP-tTA; GFAP-tTA line 78;
Mutation Made By Brian Popko,   University of Chicago
Strain of Origin(C57BL/6 x DBA/2)F1
Site of ExpressionExpresses tTA in astrocytes.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter GFAP, glial fibrillary acidic protein, human
Molecular Note Expression of the tetracycline transactivator protein is under control of the promoter of the human glial fibrillary acidic protein gene. [MGI Ref ID J:109838]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(tTA)

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as hemizygotes. It is not known if homozygotes are viable and fertile.
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of GFAP     (8 strains)

View Strains carrying other alleles of tTA     (19 strains)

Additional Web Information

Congenic Nomenclature
Tet Expression Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Astrocyte Defects
Tet Expression System (tTA/rtTA Expressing Strains)

Research Tools
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Genetics Research (Tissue/Cell Markers: astrocyte-specific marker)
Genetics Research (Tissue/Cell Markers: astrocytes)
Genetics Research (Tissue/Cell Markers: astrocytes, neurons)
Neurobiology Research (Tetop Tet System)
Neurobiology Research (cell marker)
Tet Expression Systems (tTA/rtTA Expressing Strains)

References

Selected Reference(s)

Wang J; Lin W; Popko B; Campbell IL. 2004. Inducible production of interferon-gamma in the developing brain causes cerebellar dysplasia with activation of the Sonic hedgehog pathway. Mol Cell Neurosci 27(4):489-96. [PubMed: 15555926]  [MGI Ref ID J:109838]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(GFAP-tTA)110Pop/J
Stock Number: 005964

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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