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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x +/+ sibling (Female x Male) 23-MAY-08 Species laboratory mouse Generation ?+N1F12 (30-JUN-11)
Generation DefinitionsDonating Investigator Brian Popko, University of Chicago Description
Mice hemizygous for the transgene are viable fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express a tetracycline-controlled transactivator protein (tTA) driven by the human glial fibrillary acidic protein (GFAP) promoter. When these mice are mated to a second transgenic strain that carries a target gene under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in astrocytes in the bitransgenic offspring can be induced by withdrawal of the tetracycline analog, doxycycline. Doxycycline may be administered in the animals' water supply. This strain represents an effective tool for generating bitrangenic animals that may be useful to study inducible gene expression in the astrocytes of the central nervous system.Development
A transgenic construct in which tetracycline-responsive transcriptional activator (tTA) is under the control of the human glial fibrillary acidic protein GFAP promoter was injected into fertilized B6D2F1 mouse eggs. The resulting founder line 110 was backcrossed onto the C57BL/6J background for more than 10 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of GFAP
View Strains carrying other alleles of GFAP (17 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Astrocyte Defects
Tet Expression System
tTA/rtTA Expressing Strains
Research Tools
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers
Tissue/Cell Markers: astrocyte-specific marker
Tissue/Cell Markers: astrocytes
Tissue/Cell Markers: astrocytes, neurons
Neurobiology Research
Tetop Tet System
cell marker
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(GFAP-tTA)110Pop | ||
|---|---|---|---|
| Allele Name | transgene insertion 110, Brian Popko | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | GFAP-tTA; GFAP-tTA line 110; GFAP/tTA; | ||
| Mutation Made By | Brian Popko, University of Chicago | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Site of Expression | Expresses tTA in astrocytes. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | GFAP, glial fibrillary acidic protein, human | ||
| Molecular Note | Expression of the tetracycline transactivator protein is under control of the promoter of the human glial fibrillary acidic protein gene. Five lines (67, 78, 96, 98 and 110) were established. [MGI Ref ID J:94092] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wang J; Lin W; Popko B; Campbell IL. 2004. Inducible production of interferon-gamma in the developing brain causes cerebellar dysplasia with activation of the Sonic hedgehog pathway. Mol Cell Neurosci 27(4):489-96. [PubMed: 15555926] [MGI Ref ID J:109838]
Additional References
Lin W; Harding HP; Ron D; Popko B. 2005. Endoplasmic reticulum stress modulates the response of myelinating oligodendrocytes to the immune cytokine interferon-gamma. J Cell Biol 169(4):603-12. [PubMed: 15911877] [MGI Ref ID J:99655]
Lin W; Kemper A; McCarthy KD; Pytel P; Wang JP; Campbell IL; Utset MF; Popko B. 2004. Interferon-gamma induced medulloblastoma in the developing cerebellum. J Neurosci 24(45):10074-83. [PubMed: 15537876] [MGI Ref ID J:94092]
Tg(GFAP-tTA)110Pop relatedLin W; Harding HP; Ron D; Popko B. 2005. Endoplasmic reticulum stress modulates the response of myelinating oligodendrocytes to the immune cytokine interferon-gamma. J Cell Biol 169(4):603-12. [PubMed: 15911877] [MGI Ref ID J:99655]
Lin W; Kemper A; McCarthy KD; Pytel P; Wang JP; Campbell IL; Utset MF; Popko B. 2004. Interferon-gamma induced medulloblastoma in the developing cerebellum. J Neurosci 24(45):10074-83. [PubMed: 15537876] [MGI Ref ID J:94092]
Lin W; Kunkler PE; Harding HP; Ron D; Kraig RP; Popko B. 2008. Enhanced integrated stress response promotes myelinating oligodendrocyte survival in response to interferon-gamma. Am J Pathol 173(5):1508-17. [PubMed: 18818381] [MGI Ref ID J:143347]
Lopez ME; Klein AD; Dimbil UJ; Scott MP. 2011. Anatomically defined neuron-based rescue of neurodegenerative niemann-pick type C disorder. J Neurosci 31(12):4367-78. [PubMed: 21430138] [MGI Ref ID J:170312]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. It is not known if homozygotes are viable and fertile. Mating System Hemizygote x +/+ sibling (Female x Male) 23-MAY-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(GFAP-tTA)110Pop
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(GFAP-tTA)110Pop x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(GFAP-tTA)110Pop Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(GFAP-tTA)110Pop
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(GFAP-tTA)110Pop x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(GFAP-tTA)110Pop Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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