Strain Name:

C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn/Pgn

Stock Number:

005968

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6J-Tg(Tnfsf4)2Pgn/Pgn    (Changed: 25-AUG-06 )
C57BL/6J-Tg(Tnfsf4)2Pgn    (Changed: 21-DEC-05 )
B6-TgN(OX40L) line B    (Changed: 19-DEC-05 )
Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
H2 Haplotypeb
GenerationN11p
Generation Definitions

Appearance
black
Related Genotype: a/a

Development
Mice were developed on the C57BL/6J background using a transgenic construct containing a metallothionien promoter, a beta globin IVS intron and a 0.87 Kb fragment of C57BL/6 mouse Tnfsf4 cDNA.

Related Strains

View Strains carrying other alleles of Mt1     (15 strains)

Strains carrying other alleles of Tnfsf4
002671   B6.PERA-Tnfsf4Ath1-PERA/EiJ/Pgn
005969   B6Cr.129S4-Tnfsf4tm1Sug/Pgn
005967   C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn
005075   NOD.129(B6)-Tnfsf4tm1Shr/DoiJ
View Strains carrying other alleles of Tnfsf4     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Mt1-Tnfsf4)2Pgn/?

        C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn
  • cardiovascular system phenotype
  • increased susceptibility to atherosclerosis
    • sizes of atherosclerotic lesions at the aortic roots of transgenic female mice were ~2.3-fold greater than those of their nontransgenic littermates after 16 weeks on an atherogenic diet begun at age 10 weeks   (MGI Ref ID J:97460)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • plasma levels of triglycerides, total cholesterol, VLDL cholesterol and HDL cholesterol were similar for transgenic females and their non-transgenic littermates both before and after 16 weeks on a high fat diet begun at age 10 weeks   (MGI Ref ID J:97460)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tg(Mt1-Tnfsf4)2Pgn related

Cardiovascular Research
Diet-Induced Atherosclerosis
      Susceptible

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Mt1-Tnfsf4)2Pgn
Allele Name transgene insertion 2, Beverly Paigen
Allele Type Transgenic (random, expressed)
Strain of OriginC57BL/6J
Expressed Gene Tnfsf4, tumor necrosis factor (ligand) superfamily, member 4, mouse, laboratory
Promoter Mt1, metallothionein 1, mouse, laboratory
General Note This is line 2 of four transgenic lines. According to the authors, although the line 2 was the primary one described in the reference, all four share the same phenotype.
Molecular Note The Tnfsf4 cDNA, amplified from mRNA isolated from spleens of C57BL/6J mice, was placed under control of the ubiquitously expressed metallothionein promoter followed by the hemoglobin beta IVS intron. The 3' end of the cDNA, without its polyadenylation signal, was joined to a fragment of the human growth hormone gene, which distinguishes transgene-derived from endogenous transcripts. Transgenic mice express 4- to 250-fold higher levels, depending on tissue type, of transgenic mRNA than do nontransgenic littermates in liver, aorta, heart and spleen. [MGI Ref ID J:97460]
 
 

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wang X; Ria M; Kelmenson PM; Eriksson P; Higgins DC; Samnegard A; Petros C; Rollins J; Bennet AM; Wiman B; de Faire U; Wennberg C; Olsson PG; Ishii N; Sugamura K; Hamsten A; Forsman-Semb K; Lagercrantz J; Paigen B. 2005. Positional identification of TNFSF4, encoding OX40 ligand, as a gene that influences atherosclerosis susceptibility. Nat Genet 37(4):365-72. [PubMed: 15750594]  [MGI Ref ID J:97460]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Purchasing information

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing
Order this mouse

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $1980.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing
Order this mouse

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2574.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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