Strain Name:

C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn/Pgn

Stock Number:

005968

Availability:

Repository-Cryopreserved

Description

Strain Information

Former Names C57BL/6J-Tg(Tnfsf4)2Pgn/Pgn    (Changed: 25-AUG-06 )
C57BL/6J-Tg(Tnfsf4)2Pgn    (Changed: 21-DEC-05 )
B6-TgN(OX40L) line B    (Changed: 19-DEC-05 )
Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
H2 Haplotypeb
GenerationN11p

Appearance
black
Related Genotype: a/a

Development
Mice were developed on the C57BL/6J background using a transgenic construct containing a metallothionien promoter, a beta globin IVS intron and a 0.87 Kb fragment of C57BL/6 mouse Tnfsf4 cDNA.

Related Strains

View Strains carrying other alleles of Mt1     (14 strains)

Strains carrying other alleles of Tnfsf4
002671   B6.PERA-Tnfsf4Ath1-PERA/EiJ/Pgn
005969   B6Cr.129S4-Tnfsf4tm1Sug/Pgn
005967   C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn
005075   NOD.129(B6)-Tnfsf4tm1Shr/DoiJ
View Strains carrying other alleles of Tnfsf4     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(Mt1-Tnfsf4)2Pgn/?

        C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn
  • cardiovascular system phenotype
  • increased susceptibility to atherosclerosis (MGI Ref ID J:97460)
    • sizes of atherosclerotic lesions at the aortic roots of transgenic female mice were ~2.3-fold greater than those of their nontransgenic littermates after 16 weeks on an atherogenic diet begun at age 10 weeks
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype (MGI Ref ID J:97460)
    • plasma levels of triglycerides, total cholesterol, VLDL cholesterol and HDL cholesterol were similar for transgenic females and their non-transgenic littermates both before and after 16 weeks on a high fat diet begun at age 10 weeks
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Mt1 related

Metabolism Research

Tg(Mt1-Tnfsf4)2Pgn related

Cardiovascular Research
Diet-Induced Atherosclerosis (Susceptible)

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Mt1-Tnfsf4)2Pgn
Allele Name transgene insertion 1, Beverly Paigen
Allele Type Transgenic (random, expressed)
Strain of OriginC57BL/6J
Expressed Gene Tnfsf4, tumor necrosis factor (ligand) superfamily, member 4, mouse, laboratory
Promoter Mt1, metallothionein 1, mouse, laboratory
General Note This is line 2 of four transgenic lines. According to the authors, although the line 2 was the primary one described in the reference, all four share the same phenotype.
Molecular Note The Tnfsf4 cDNA, amplified from mRNA isolated from spleens of C57BL/6J mice, was placed under control of the ubiquitously expressed metallothionein promoter followed by the hemoglobin beta IVS intron. The 3' end of the cDNA, without its polyadenylation signal, was joined to a fragment of the human growth hormone gene, which distinguishes transgene-derived from endogenous transcripts. Transgenic mice express 4- to 250-fold higher levels, depending on tissue type, of transgenic mRNA than do nontransgenic littermates in liver, aorta, heart and spleen. [MGI Ref ID J:97460]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Wang X; Ria M; Kelmenson PM; Eriksson P; Higgins DC; Samnegard A; Petros C; Rollins J; Bennet AM; Wiman B; de Faire U; Wennberg C; Olsson PG; Ishii N; Sugamura K; Hamsten A; Forsman-Semb K; Lagercrantz J; Paigen B. 2005. Positional identification of TNFSF4, encoding OX40 ligand, as a gene that influences atherosclerosis susceptibility. Nat Genet 37(4):365-72. [PubMed: 15750594]  [MGI Ref ID J:97460]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions


See Terms of Use


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

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