Strain Name: |
C3H/HeJBirLtJ |
|---|---|
Stock Number: |
005972 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Pde6b; Pde6brd1; Tlr4; Tlr4Lps-d; |
Product Information
Strain Details
Type Inbred Strain Additional information on Inbred Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Additional information on JAX® GEMM® Strains. Species laboratory mouse H2 Haplotype k Generation ?+F23p Appearance
agouti
Related Genotype: A/AImportant Note
This strain is homozygous for Pde6brd1 and Tlr4Lps-d.Strain Description
Under conventional housing conditions, C3H/HeJBirLtJ mice develop spontaneous colitis. It should be noted that the phenotype of cecocolitis in this strain requires an interaction with an as yet undefined component of the enteric flora. Inflammation is present mainly in the cecum and right colon. Colitis develops early in life and resolves by three months of age. The colitis is characterized by acute and chronic inflammation, ulcerations, crypt abscesses, regenerative hyperplasia and submucosal scarring. A mild recurrence of the disease can occur after one year. Small lesions at the anorectal junction are common throughout life.Strain Development
C3H/HeJ mice (at F204-208) were initially obtained from The Jackson Laboratory production facility. Mice were selectively bred for a high incidence of perianal ulceration, soft feces and positive Hemoccult test results. Over a period of a year, beginning in 1992, a pedigreed substrain was established starting with one severely affected female and one clinically normal male. Once the colony was established, affected mice were sibling mated and the pedigree with the highest incidence of disease was selected for further study. (Sundberg JP, et al., 1994) The new substrain was developed in the laboratory of Dr. Edward Birkenmeier and named C3H/HeJBir. The colony was transferred to Dr. Ed Leiter in the mid 1990's. The Jackson Laboratory Repository imported this strain from the research laboratory of Dr. Ed Leiter in 2006 at generation F52.
Gene & Allele Details
| Allele Symbol | Pde6brd1 | ||
|---|---|---|---|
| Allele Name | retinal degeneration 1 | ||
| Common Name(s) | rd; rd-1; rd1; rodless retina; | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
| Molecular Note | Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C to A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain has also been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested. [MGI Ref ID J:11513] [MGI Ref ID J:4366] [MGI Ref ID J:51361] | ||
| Allele Symbol | Tlr4Lps-d | ||
| Allele Name | defective lipopolysaccharide response | ||
| Common Name(s) | TLR4-Mu; Tlr4-; Tlr4d; TlrLps-d; lpsd; | ||
| Strain of Origin | C3H/HeJ | ||
| Gene Symbol and Name | Tlr4, toll-like receptor 4 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | ARMD10; CD284; Lps; RAS-like, family 2, locus 8; Rasl2-8; TOLL; hToll; lipopolysaccharide response; | ||
| General Note |
C3H/HeJ mice carry this allele. Various combinations of Lps-associated traits have been followed in crosses between C3H/HeJ and other C3H substrains, and the traits have in all cases segregated together (J:30692, J:5557, J:5593, J:5938). Some of the traits show dominance of the Tlr4lps-n allele; others, including Tlr4Lps-d, show codominance. Genbank ID for this allele: AF095353 | ||
| Molecular Note | This allele corresponds to a mutation in the third exon of the gene. The substitution of an A to C at nucleotide position 2342, results in an amino acid substitution that replaces proline with histidine at position 712. [MGI Ref ID J:51522] [MGI Ref ID J:53519] [MGI Ref ID J:57938] | ||
Related Strains
C3H Strains
000659 C3H/HeJ 001824 C3H/HeJSxJ 000635 C3H/HeOuJ 000474 C3H/HeSn 000661 C3H/HeSnJ 000658 C3HeB/FeJ 001908 C3HfB/BiJ View C3H Strains (7 strains)
002930 C.C3-Tlr4Lps-d/J 005973 C3Bir.129P2(B6)-Il10C3Bir/LtJ 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 003968 C3Bir.129P2(B6)-Il10tm1Cgn/LtJ 000659 C3H/HeJ
004297 B6.CXB1-Pde6brd10/J 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
007227 B6.B10ScN-Tlr4lps-del/JthJ 003752 C57BL/10ScNJ
Phenotypic Data
Festing Inbred Strain Characteristics: C3H
Additional Web Information
JAX® NOTES, Spring 2002; 485. Genetic Background Effects: Can Your Mice See?
Research Applications
This mouse can be used to support research in many areas including:
Pde6brd1 relatedImmunology and Inflammation Research
Inflammation (Inflammatory bowel disease)
Internal/Organ Research
Gastrointestinal Defects (colitis)
Tlr4Lps-d relatedMouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (Tlr deficiency)
Immunodeficiency (Tlr deficiency)
Inflammation (Tlr deficiency)
References
Additional ReferencesPrice and Supply Information
| Strain Name: | C3H/HeJBirLtJ |
| Stock Number: | 005972 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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