Strain Name: |
C3Bir.129P2(B6)-Il10C3Bir/LtJ |
|---|---|
Stock Number: |
005973 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Il10; Pde6b; Pde6brd1; Tlr4; Tlr4Lps-d; ; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Species laboratory mouse Background Strain C3H/HeJBirLt Donor Strain 129P2/OlaHsd via B6.129P2-Il10tm1Cgn/J H2 Haplotype k Appearance
agouti
Related Genotype: A/AImportant Note
This strain is homozygous for Pde6brd1 and Tlr4Lps-d.Strain Description
This is a control strain for C3Bir.129P2(B6)-Il10tm1Cgn/Lt (Stock No. 004326), developed in parallel with the latter until backcrossing was complete. As such, it does not exhibit the Il10-deficient phenotype.Strain Development
This is a control strain for C3Bir.129P2(B6)-Il10tm1Cgn/Lt (Stock No. 004326), developed in parallel with the latter until backcrossing was complete.The Il10tm1Cgn mutation was created by in the Laboratory of Dr. Werner Muller at the University of Cologne. Briefly, a targeting vector was designed to replace codons 5-55 of exon 1 of the targeted gene with a 24 bp linker (providing a termination codon) and a neo expression cassette, as well as introduce a termination codon into exon 3. The construct was electroporated into 129P2/OlaHsd-derived E14-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females to establish the mutant colony on a mixed B6;129P2 genetic background. It was then imported to the Induced Mutant Resource at The Jackson Laboratory in 1995. Subsequently, mutant mice were backcrossed to the C3H/HeJBirLt host strain for at least 10 generations. At the tenth backcross generation, heterozygotes were intercrossed to produce founders for a homozygous mutant line (Stock No. 004326) as well as founders for this wildtype control line (Stock No. 005973). This strain was imported into The Jackson Laboratory Repository at N15F15 from the research laboratory of Dr. Ed Leiter in 2006.
Gene & Allele Details
| Gene Symbol and Name | Il10, interleukin 10 | ||
|---|---|---|---|
| Chromosome | 1 | ||
| Gene Common Name(s) | CSIF; IL-10; IL10A; IL10X; Il-10; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Common Name(s) | rd; rd-1; rd1; rodless retina; | ||
| Allele Symbol | Tlr4Lps-d | ||
| Allele Name | defective lipopolysaccharide response | ||
| Common Name(s) | TLR4-Mu; Tlr4-; Tlr4d; TlrLps-d; lpsd; | ||
| Strain of Origin | C3H/HeJ | ||
| Gene Symbol and Name | Tlr4, toll-like receptor 4 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | ARMD10; CD284; Lps; RAS-like, family 2, locus 8; Rasl2-8; TOLL; hToll; lipopolysaccharide response; | ||
| General Note |
C3H/HeJ mice carry this allele. Various combinations of Lps-associated traits have been followed in crosses between C3H/HeJ and other C3H substrains, and the traits have in all cases segregated together (J:30692, J:5557, J:5593, J:5938). Some of the traits show dominance of the Tlr4lps-n allele; others, including Tlr4Lps-d, show codominance. Genbank ID for this allele: AF095353 | ||
| Molecular Note | This allele corresponds to a mutation in the third exon of the gene. The substitution of an A to C at nucleotide position 2342, results in an amino acid substitution that replaces proline with histidine at position 712. [MGI Ref ID J:51522] [MGI Ref ID J:53519] [MGI Ref ID J:57938] | ||
Control Information
| This strain is a control for 004326 and 003968. | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. |
|---|
Related Strains
Strains carrying Pde6brd1 allele
View Strains carrying Pde6brd1 (74 strains)
002930 C.C3-Tlr4Lps-d/J 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 003968 C3Bir.129P2(B6)-Il10tm1Cgn/LtJ 000659 C3H/HeJ 005972 C3H/HeJBirLtJ
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Pde6brd1 relatedImmunology and Inflammation Research
Inflammation (control strain)
Tlr4Lps-d relatedMouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (Tlr deficiency)
Immunodeficiency (Tlr deficiency)
Inflammation (Tlr deficiency)
References
Additional ReferencesPrice and Supply Information
| Strain Name: | C3Bir.129P2(B6)-Il10C3Bir/LtJ |
| Stock Number: | 005973 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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