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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(Plp1-cre/ESR1)3Pop/J (Changed: 30-MAR-10 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x +/+ sibling (Female x Male) 16-APR-08 Species laboratory mouse Generation N10+N1F12 (30-JUN-11)
Generation DefinitionsDonating Investigator Brian Popko, University of Chicago Description
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Immunohistochemical analysis of brain, optic and sciatic nerve tissue reveals that the cre/Esr1 expression mimics the endogenous promoter expression pattern.Development
A transgenic construct containing a 2.4 kb sequence of mouse proteolipid protein (myelin) 1 promoter, sequence encoding cre/Esr1 and SV40 polyadenylation site sequence was injected into B6D2F1 donor eggs. Founder line 3 was subsequently established. Founder animals were bred to C57BL/6J mice and then backcrossed to the same for 10 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of ESR1
View Strains carrying other alleles of ESR1 (28 strains)
003255 B6;129-Plp1tm1Kan/J 000287 B6CBACa Aw-J/A-Plp1jp EdaTa/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Plp1-cre/ERT)3Pop/0
involves: C57BL/6 * DBA/2
- normal phenotype
- no abnormal phenotype detected
- mice ae fertile and appear normal; expression of cre is primarily restricted to myelinating oligodendrocytes of the CNS (MGI Ref ID J:81627)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Plp1-cre/ERT)3Pop | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Brian Popko | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | PLP/CreER; Plp-Cre-ERT2; Plp-CreERt; Tg(Plp-cre/ESR1)3.16Pop; Tg(Plp1-cre/ESR1)3.16Pop; Tg(Plp1-cre/ESR1)3Pop; | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Site of Expression | tamoxifen inducible Cre recombinase protein fused to mutant form of the mouse estrogen receptor ligand binding domain; expression in oligodendrocytes and Schwann cells | ||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Plp1, proteolipid protein (myelin) 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Plp1-cre/ERT)3Pop, transgene insertion 3, Brian Popko | ||
| Chromosome | UN | ||
| Gene Common Name(s) | PLP/CreER; Tg(Plp-cre/ESR1)3.16Pop; Tg(Plp1-cre/ESR1)3.16Pop; Tg(Plp1-cre/ESR1)3Pop; | ||
| Driver Note | Plp1 | ||
| Inducible Note | Induced by tamoxifen. | ||
| Molecular Note | The transgenic construct encodes a tamoxifen inducible fusion protein consisting of cre recombinase and a mutated human estrogen receptor ligand binding domain cloned next to a Plp promoter. The 2.5 kb Plp regulatory region includes 5' flanking DNA, exon1, and intron 1. Transgene expression was confirmed by RT-PCR and Western blot analysis. The mouse Plp promoter was used to drive transgene expression in myelinating cells. [MGI Ref ID J:81627] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Tg(Plp1-cre/ESR1)3.16Pop, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Doerflinger NH; Macklin WB; Popko B. 2003. Inducible site-specific recombination in myelinating cells. Genesis 35(1):63-72. [PubMed: 12481300] [MGI Ref ID J:81627]
Additional References
Tg(Plp1-cre/ERT)3Pop relatedGomez-Casati ME; Murtie JC; Rio C; Stankovic K; Liberman MC; Corfas G. 2010. Nonneuronal cells regulate synapse formation in the vestibular sensory epithelium via erbB-dependent BDNF expression. Proc Natl Acad Sci U S A 107(39):17005-10. [PubMed: 20837532] [MGI Ref ID J:164570]
Guo F; Ma J; McCauley E; Bannerman P; Pleasure D. 2009. Early postnatal proteolipid promoter-expressing progenitors produce multilineage cells in vivo. J Neurosci 29(22):7256-70. [PubMed: 19494148] [MGI Ref ID J:155647]
Guo F; Maeda Y; Ko EM; Delgado M; Horiuchi M; Soulika A; Miers L; Burns T; Itoh T; Shen H; Lee E; Sohn J; Pleasure D. 2012. Disruption of NMDA Receptors in Oligodendroglial Lineage Cells Does Not Alter Their Susceptibility to Experimental Autoimmune Encephalomyelitis or Their Normal Development. J Neurosci 32(2):639-45. [PubMed: 22238099] [MGI Ref ID J:179903]
He Y; Kim JY; Dupree J; Tewari A; Melendez-Vasquez C; Svaren J; Casaccia P. 2010. Yy1 as a molecular link between neuregulin and transcriptional modulation of peripheral myelination. Nat Neurosci 13(12):1472-80. [PubMed: 21057508] [MGI Ref ID J:166894]
Kang SH; Fukaya M; Yang JK; Rothstein JD; Bergles DE. 2010. NG2+ CNS glial progenitors remain committed to the oligodendrocyte lineage in postnatal life and following neurodegeneration. Neuron 68(4):668-81. [PubMed: 21092857] [MGI Ref ID J:167748]
Mayes DA; Rizvi TA; Cancelas JA; Kolasinski NT; Ciraolo GM; Stemmer-Rachamimov AO; Ratner N. 2011. Perinatal or Adult Nf1 Inactivation Using Tamoxifen-Inducible PlpCre Each Cause Neurofibroma Formation. Cancer Res 71(13):4675-85. [PubMed: 21551249] [MGI Ref ID J:173625]
Michalski JP; Anderson C; Beauvais A; De Repentigny Y; Kothary R. 2011. The Proteolipid Protein Promoter Drives Expression outside of the Oligodendrocyte Lineage during Embryonic and Early Postnatal Development. PLoS One 6(5):e19772. [PubMed: 21572962] [MGI Ref ID J:172435]
Pillai AM; Thaxton C; Pribisko AL; Cheng JG; Dupree JL; Bhat MA. 2009. Spatiotemporal ablation of myelinating glia-specific neurofascin (Nfasc(NF155)) in mice reveals gradual loss of paranodal axoglial junctions and concomitant disorganization of axonal domains. J Neurosci Res :. [PubMed: 19185024] [MGI Ref ID J:145846]
Shin D; Shin JY; McManus MT; Ptacek LJ; Fu YH. 2009. Dicer ablation in oligodendrocytes provokes neuronal impairment in mice. Ann Neurol 66(6):843-57. [PubMed: 20035504] [MGI Ref ID J:162057]
Viader A; Chang LW; Fahrner T; Nagarajan R; Milbrandt J. 2011. MicroRNAs Modulate Schwann Cell Response to Nerve Injury by Reinforcing Transcriptional Silencing of Dedifferentiation-Related Genes. J Neurosci 31(48):17358-69. [PubMed: 22131398] [MGI Ref ID J:178143]
Zhou YX; Pannu R; Le TQ; Armstrong RC. 2012. Fibroblast growth factor 1 (FGFR1) modulation regulates repair capacity of oligodendrocyte progenitor cells following chronic demyelination. Neurobiol Dis 45(1):196-205. [PubMed: 21854849] [MGI Ref ID J:179847]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. Mating System Hemizygote x +/+ sibling (Female x Male) 16-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Plp1-cre/ERT)3Pop
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Plp1-cre/ERT)3Pop x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Plp1-cre/ERT)3Pop Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
|
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Plp1-cre/ERT)3Pop
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Plp1-cre/ERT)3Pop x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Plp1-cre/ERT)3Pop Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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