Strain Name: |
B6.Cg-Tg(Plp1-cre/ESR1)3.16Pop/J |
|---|---|
Stock Number: |
005975 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | ESR1; Plp1; cre; Tg(Plp1-cre/ESR1)3.16Pop; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Brian Popko, University of Chicago Generation N10+1F4 (08-JAN-08) Strain Description
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Immunohistochemical analysis of brain, optic and sciatic nerve tissue reveals that the cre/Esr1 expression mimics the endogenous promoter expression pattern.Strain Development
A transgenic construct containing a 2.4 kb sequence of mouse proteolipid protein (myelin) 1 promoter, sequence encoding cre/Esr1 and SV40 polyadenylation site sequence was injected into B6D2F1 donor eggs. Founder animals were bred to C57BL/6J mice and then backcrossed to the same for 10 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Plp1-cre/ESR1)3.16Pop | ||
|---|---|---|---|
| Allele Name | transgene insertion 3.16, Brian Popko | ||
| Common Name(s) | PLP/CreER; Tg(Plp-cre/ESR1)3.16Pop; | ||
| Site of Expression | tamoxifen inducible Cre recombinase protein fused to mutant form of the mouse estrogen receptor ligand binding domain; expression in oligodendrocytes and Schwann cells | ||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Plp1, proteolipid protein (myelin) 1, mouse, laboratory | ||
| Molecular Note | The transgenic construct encodes a tamoxifen inducible fusion protein consisting of cre recombinase and a mutated human estrogen receptor ligand binding domain cloned next to a Plp promoter. The 2.5 kb Plp regulatory region includes 5' flanking DNA, exon1, and intron 1. Transgene expression was confirmed by RT-PCR and Western blot analysis. The mouse Plp promoter was used to drive transgene expression in myelinating cells. [MGI Ref ID J:81627] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of ESR1
View Strains carrying other alleles of ESR1 (7 strains)
003255 B6;129-Plp1tm1Kan/J 000287 B6CBACa Aw-J/A-Plp1jp EdaTa/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Doerflinger NH; Macklin WB; Popko B. 2003. Inducible site-specific recombination in myelinating cells. Genesis 35(1):63-72. [PubMed: 12481300] [MGI Ref ID J:81627]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Plp1-cre/ESR1)3.16Pop/J |
| Stock Number: | 005975 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(Plp1-cre/ESR1)3.16Pop | |||
| Pair | $288.65 | Hemizygous for Tg(Plp1-cre/ESR1)3.16Pop x Noncarrier | |||
| Pair | $288.65 | Noncarrier x Hemizygous for Tg(Plp1-cre/ESR1)3.16Pop | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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