Description

Strain Information

Former Names NOD.129S4(B6)-Icam1tm1Jcgr/MxJ    (Changed: 27-FEB-06 ) NOD.129S7(B6)-Icam1tm1Bay/MxJ    (Changed: 24-JAN-06 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain NOD Donor Strain 129S4 (J1 ES cell) H2 Haplotype g7 Generation N?+F3pN1   Donating Investigator Stephan Martin,   Dusseldorf at the Heinrich-Heine-Univers

Description
No membrane bound ICAM-1 is detectable in NOD.129S4(B6)-Icam1^tm1Jcgr/SmtnJ homozygous mice. NOD mice homozygous for the Icam1^tm1Jcgr mutation are diabetes resistant. Histological exam shows no or mild peri-insular infiltration at 330-410 days of age, while 64% of their wild-type cohorts and 44% of the heterozygote animals become diabetic by 310 days of age. In contrast to wild-type, 110-day-old ICAM-1 deficient females do not develop severe insulitis 10 days post cyclophosphamide treatment. Protection is associated with suppression of destructive insulitis as well as a deviation to a T-helper 2 cytokine mRNA expression pattern (Martin et al. 2001).

Both the C57BL/6 contaminated strain and the non contaminated strain, Stock No. 006351 - NOD.129S4(B6)-Icam1^tm1Jcgr/J are completely diabetes resistant at 30 weeks of age when evaluated in a comparative diabetes incidence study. Fine mapping data may be viewed at GQC of Icam1 congenic stocks

NOD.129S4(B6)-Icam1^tm1Jcgr/SmtnJ is useful for studying the ICAM-1/LAF-1 interaction and its role in diabetes.

Development
Intercellular adhesion molecule 1 (Icam1), located on Chr9, 7cM, contains 7 exons and is one of the immunoglobulin superfamily that binds to the integrins LFA-1 and Mac-1 on leukocytes. A neomycin resistance cassette was inserted into exon 4 (one of the 5 Ig-like extracellular domains) of the Icam1 gene. J1 ES cell clone 74 was injected into B6 blastocysts. This Icam1 mutation has been backcrossed to NOD for at least 6 generations (Martin et al, 2001). The Type 1 Diabetes Resource received NOD.129S4(B6)-Icam1^tm1Jcgr/SmtnJ in 2005.

Additional genetic quality control performed at The Jackson Laboratory indicates C57BL/6 genomic contamination on Chromosomes 6 and 7. Markers D6Mit178, D6Mit52 and D6Mit135 on Chromosome 6 were segregating for both NOD and C57BL/6. Through selective breeding we have been able to make the Chromosome 6 markers of NOD origin only. Markers D7Mit139, D7Mit96 and D7Mit328 on Chromosome 7 were found to be of C57BL/6 origin. This stock is available with the Chromosome 7 genomic contamination and may affect the diabetes incidence.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Related Strains

Strains carrying   Icam1^tm1Jcgr allele

002867	B6.129S4-Icam1tm1Jcgr/J
006351	NOD.129S4(B6)-Icam1tm1Jcgr/J

View Strains carrying   Icam1^tm1Jcgr     (2 strains)

Strains carrying other alleles of Icam1

002285	B6.129S7-Icam1tm1Bay Selptm1Bay/J
002127	B6.129S7-Icam1tm1Bay/J

View Strains carrying other alleles of Icam1     (2 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Icam1^tm1Jcgr related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation

Internal/Organ Research
Wound Healing (delayed/impaired)

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol		Icam1tm1Jcgr
Allele Name		targeted mutation 1, Jose Carlos Gutierrez Ramos
Allele Type		Targeted (knock-out)
Common Name(s)		ICAM-1-; ICAM-1-deficient; ICAMtm1-jcgr;
Mutation Made By		Jose-Carlos Gutierrez-Ramos,   Millenium Pharmaceuticals
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Icam1, intercellular adhesion molecule 1
Chromosome		9
Gene Common Name(s)		BB2; CD54; ICAM; Icam-1; Ly-47; MALA-2; MGC:6195; P3.58; lymphocyte antigen 47;
Molecular Note		A neomycin resistance cassette was inserted into exon 4 of the gene. While King, et. al. (J:25530) reported the potential for the production of functional proteins arising from alternative transcripts, corresponding signals were undetected by immunohistochemical analysis of homozygous mutant mice. [MGI Ref ID J:18743]

Genotyping

Genotyping Information

Genotyping Protocols

Icam1^tm1Jcgr, STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Martin S; van Den Engel NK; Vinke A; Heidenthal E; Schulte B; Kolb H. 2001. Dominant role of intercellular adhesion molecule-1 in the pathogenesis of autoimmune diabetes in non-obese diabetic mice. J Autoimmun 17(2):109-17. [PubMed: 11591119]  [MGI Ref ID J:71827]

Additional References

Icam1^tm1Jcgr related

Aoudjit F; Potworowski EF; Springer TA; St-Pierre Y. 1998. Protection from lymphoma cell metastasis in ICAM-1 mutant mice: a posthoming event. J Immunol 161(5):2333-8. [PubMed: 9725228]  [MGI Ref ID J:49717]

Balasa B; La Cava A; Van Gunst K; Mocnik L; Balakrishna D; Nguyen N; Tucker L; Sarvetnick N. 2000. A mechanism for IL-10-mediated diabetes in the nonobese diabetic (NOD) mouse: ICAM-1 deficiency blocks accelerated diabetes J Immunol 165(12):7330-7. [PubMed: 11120869]  [MGI Ref ID J:66103]

Chen Q; Fisher DT; Clancy KA; Gauguet JM; Wang WC; Unger E; Rose-John S; von Andrian UH; Baumann H; Evans SS. 2006. Fever-range thermal stress promotes lymphocyte trafficking across high endothelial venules via an interleukin 6 trans-signaling mechanism. Nat Immunol 7(12):1299-1308. [PubMed: 17086187]  [MGI Ref ID J:116128]

Cybulsky MI; Iiyama K; Li H; Zhu S; Chen M; Iiyama M; Davis V; Gutierrez-Ramos JC; Connelly PW; Milstone DS. 2001. A major role for VCAM-1, but not ICAM-1, in early atherosclerosis. J Clin Invest 107(10):1255-62. [PubMed: 11375415]  [MGI Ref ID J:69597]

Dautigny N; Le Campion A; Lucas B. 1999. Timing and casting for actors of thymic negative selection. J Immunol 162(3):1294-302. [PubMed: 9973382]  [MGI Ref ID J:124433]

Davani EY; Boyd JH; Dorscheid DR; Wang Y; Meredith A; Chau E; Singhera GK; Walley KR. 2006. Cardiac ICAM-1 mediates leukocyte-dependent decreased ventricular contractility in endotoxemic mice. Cardiovasc Res 72(1):134-42. [PubMed: 16934241]  [MGI Ref ID J:114093]

Demers M; Magnaldo T; St-Pierre Y. 2005. A novel function for galectin-7: promoting tumorigenesis by up-regulating MMP-9 gene expression. Cancer Res 65(12):5205-10. [PubMed: 15958565]  [MGI Ref ID J:99384]

Dong ZM; Gutierrez-Ramos JC; Coxon A; Mayadas TN; Wagner DD. 1997. A new class of obesity genes encodes leukocyte adhesion receptors. Proc Natl Acad Sci U S A 94(14):7526-30. [PubMed: 9207125]  [MGI Ref ID J:41751]

Feuillet V; Lucas B; Di Santo JP; Bismuth G; Trautmann A. 2005. Multiple survival signals are delivered by dendritic cells to naive CD4+ T cells. Eur J Immunol 35(9):2563-72. [PubMed: 16078277]  [MGI Ref ID J:113487]

Frossard JL; Saluja A; Bhagat L; Lee HS; Bhatia M; Hofbauer B; Steer ML. 1999. The role of intercellular adhesion molecule 1 and neutrophils in acute pancreatitis and pancreatitis-associated lung injury. Gastroenterology 116(3):694-701. [PubMed: 10029629]  [MGI Ref ID J:107680]

Gonzalo JA; Martinez C; Springer TA; Gutierrez-Ramos JC. 1995. ICAM-1 is required for T cell proliferation but not for anergy or apoptosis induced by Staphylococcus aureus enterotoxin B in vivo. Int Immunol 7(10):1691-8. [PubMed: 8562515]  [MGI Ref ID J:29453]

Gujral JS; Liu J; Farhood A; Hinson JA; Jaeschke H. 2004. Functional importance of ICAM-1 in the mechanism of neutrophil-induced liver injury in bile duct-ligated mice. Am J Physiol Gastrointest Liver Physiol 286(3):G499-507. [PubMed: 14563671]  [MGI Ref ID J:95678]

Hirahashi J; Mekala D; Van Ziffle J; Xiao L; Saffaripour S; Wagner DD; Shapiro SD; Lowell C; Mayadas TN. 2006. Mac-1 signaling via Src-family and Syk kinases results in elastase-dependent thrombohemorrhagic vasculopathy. Immunity 25(2):271-83. [PubMed: 16872848]  [MGI Ref ID J:113463]

Kelly KJ; Williams WW Jr.; Colvin RB; Meehan SM; Springer TA; Gutierrez-Ramos JC; Bonventre JV. 1996. Intercellular adhesion molecule-1-deficient mice are protected against ischemic renal injury. J Clin Invest 97(4):1056-63. [PubMed: 8613529]  [MGI Ref ID J:31435]

King PD; Sandberg ET; Selvakumar A; Fang P; Beaudet AL; Dupont B. 1995. Novel isoforms of murine intercellular adhesion molecule-1 generated by alternative RNA splicing. J Immunol 154(11):6080-6093. [PubMed: 7751650]  [MGI Ref ID J:25530]

Kupatt C; Wichels R; Horstkotte J; Krombach F; Habazettl H; Boekstegers P. 2002. Molecular mechanisms of platelet-mediated leukocyte recruitment during myocardial reperfusion. J Leukoc Biol 72(3):455-61. [PubMed: 12223512]  [MGI Ref ID J:124451]

Lloyd CM; Gonzalo JA; Salant DJ; Just J; Gutierrez-Ramos JC. 1997. Intercellular adhesion molecule-1 deficiency prolongs survival and protects against the development of pulmonary inflammation during murine lupus. J Clin Invest 100(5):963-71. [PubMed: 9276713]  [MGI Ref ID J:111466]

Lyck R; Reiss Y; Gerwin N; Greenwood J; Adamson P; Engelhardt B. 2003. T-cell interaction with ICAM-1/ICAM-2 double-deficient brain endothelium in vitro: the cytoplasmic tail of endothelial ICAM-1 is necessary for transendothelial migration of T cells. Blood 102(10):3675-83. [PubMed: 12893765]  [MGI Ref ID J:86457]

Martin S; Vinke A; Heidenthal E; Schulte B; van den Engel N. 1999. Development of low-dose streptozotocin-induced diabetes in ICAM-1-deficient mice. Horm Metab Res 31(12):636-40. [PubMed: 10668914]  [MGI Ref ID J:59947]

Mathew B; Park GY; Cao H; Azim AC; Wang X; Van Breemen RB; Sadikot RT; Christman JW. 2007. Inhibitory kappaB kinase 2 activates airway epithelial cells to stimulate bone marrow macrophages. Am J Respir Cell Mol Biol 36(5):562-72. [PubMed: 17204585]  [MGI Ref ID J:136649]

Moreira AP; Campanelli AP; Cavassani KA; Souto JT; Ferreira BR; Martinez R; Rossi MA; Silva JS. 2006. Intercellular adhesion molecule-1 is required for the early formation of granulomas and participates in the resistance of mice to the infection with the fungus Paracoccidioides brasiliensis. Am J Pathol 169(4):1270-81. [PubMed: 17003484]  [MGI Ref ID J:113377]

Oliveira-dos-Santos AJ; Penninger JM; Rieker-Geley T; Matsumoto G; Mak TM; Wick G. 1998. Thymic heterotypic cellular complexes in gene-targeted mice with defined blocks in T cell development and adhesion molecule expression. Eur J Immunol 28(9):2882-92. [PubMed: 9754575]  [MGI Ref ID J:49877]

Parameswaran N; Suresh R; Bal V; Rath S; George A. 2005. Lack of ICAM-1 on APCs during T cell priming leads to poor generation of central memory cells. J Immunol 175(4):2201-11. [PubMed: 16081787]  [MGI Ref ID J:107514]

Planck SR; Han YB; Park JM; O'Rourke L; Gutierrez-Ramos JC; Rosenbaum JT. 1998. The effect of genetic deficiency of adhesion molecules on the course of endotoxin-induced uveitis. Curr Eye Res 17(9):941-6. [PubMed: 9746442]  [MGI Ref ID J:114206]

Scholer A; Hugues S; Boissonnas A; Fetler L; Amigorena S. 2008. Intercellular adhesion molecule-1-dependent stable interactions between T cells and dendritic cells determine CD8+ T cell memory. Immunity 28(2):258-70. [PubMed: 18275834]  [MGI Ref ID J:132214]

Segura E; Nicco C; Lombard B; Veron P; Raposo G; Batteux F; Amigorena S; Thery C. 2005. ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming. Blood 106(1):216-23. [PubMed: 15790784]  [MGI Ref ID J:107454]

Shimada Y; Hasegawa M; Kaburagi Y; Hamaguchi Y; Komura K; Saito E; Takehara K; Steeber DA; Tedder TF; Sato S. 2003. L-selectin or ICAM-1 deficiency reduces an immediate-type hypersensitivity response by preventing mast cell recruitment in repeated elicitation of contact hypersensitivity. J Immunol 170(8):4325-34. [PubMed: 12682269]  [MGI Ref ID J:125436]

Siebert H; Bruck W. 2003. The role of cytokines and adhesion molecules in axon degeneration after peripheral nerve axotomy: a study in different knockout mice. Brain Res 960(1-2):152-6. [PubMed: 12505667]  [MGI Ref ID J:107780]

Soriano SG; Lipton SA; Wang YF; Xiao M; Springer TA; Gutierrez-Ramos JC; Hickey PR. 1996. Intercellular adhesion molecule-1-deficient mice are less susceptible to cerebral ischemia-reperfusion injury. Ann Neurol 39(5):618-24. [PubMed: 8619547]  [MGI Ref ID J:33920]

Sumagin R; Sarelius IH. 2007. A role for ICAM-1 in maintenance of leukocyte-endothelial cell rolling interactions in inflamed arterioles. Am J Physiol Heart Circ Physiol 293(5):H2786-98. [PubMed: 17704289]  [MGI Ref ID J:132086]

Takaoka N; Campbell LA; Lee A; Rosenfeld ME; Kuo CC. 2008. Chlamydia pneumoniae infection increases adherence of mouse macrophages to mouse endothelial cells in vitro and to aortas ex vivo. Infect Immun 76(2):510-4. [PubMed: 18070891]  [MGI Ref ID J:130335]

Tang T; Rosenkranz A; Assmann KJM; Goodman MJ; Gutierrez-Ramos JC ; Carroll MC ; Cotran RS ; Mayadas TN. 1997. A role for Mac-1 (CDIIb/CD18) in immune complex-stimulated neutrophil function in vivo: Mac-1 deficiency abrogates sustained Fcgamma receptor-dependent neutrophil adhesion and complement-dependent proteinuria in acute glomerulonephritis. J Exp Med 186(11):1853-63. [PubMed: 9382884]  [MGI Ref ID J:44403]

TeKippe M; Harrison DE; Chen J. 2003. Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice. Exp Hematol 31(6):521-7. [PubMed: 12829028]  [MGI Ref ID J:115677]

Tommerup N; Schempp W; Meinecke P; Pedersen S; Bolund L; Brandt C; Goodpasture C; Guldberg P; Held KR; Reinwein H; Saugstad OD; Scherer G; Skjeldal O; Toder R; Westvik J; Van der Hagen CB; Wolf U.. 1993. Assignment of an autosomal sex reversal locus (SRA1) and campomelic dysplasia (CMPD1) to 17q24.3-q25.1. Nat Genet 4(2):170-4. [PubMed: 8348155]  [MGI Ref ID J:11820]

Walter MJ; Morton JD; Kajiwara N; Agapov E; Holtzman MJ. 2002. Viral induction of a chronic asthma phenotype and genetic segregation from the acute response. J Clin Invest 110(2):165-75. [PubMed: 12122108]  [MGI Ref ID J:120696]

Wang HW; Babic AM; Mitchell HA; Liu K; Wagner DD. 2005. Elevated soluble ICAM-1 levels induce immune deficiency and increase adiposity in mice. FASEB J 19(8):1018-20. [PubMed: 15817637]  [MGI Ref ID J:128252]

Watts GM; Beurskens FJ; Martin-Padura I; Ballantyne CM; Klickstein LB; Brenner MB; Lee DM. 2005. Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol 174(6):3668-75. [PubMed: 15749905]  [MGI Ref ID J:97703]

Werner A; Martin S; Gutierrez-Ramos JC; Raivich G. 2001. Leukocyte recruitment and neuroglial activation during facial nerve regeneration in ICAM-1-deficient mice: effects of breeding strategy. Cell Tissue Res 305(1):25-41. [PubMed: 11512669]  [MGI Ref ID J:105085]

Xiang J; Huang H; Liu Y. 2005. A new dynamic model of CD8+ T effector cell responses via CD4+ T helper-antigen-presenting cells. J Immunol 174(12):7497-505. [PubMed: 15944248]  [MGI Ref ID J:100792]

Xu H; Gonzalo JA; St Pierre Y; Williams IR; Kupper TS; Cotran RS; Springer TA; Gutierrez-Ramos JC. 1994. Leukocytosis and resistance to septic shock in intercellular adhesion molecule 1-deficient mice. J Exp Med 180(1):95-109. [PubMed: 7911822]  [MGI Ref ID J:18743]

van Den Engel NK; Heidenthal E; Vinke A; Kolb H; Martin S. 2000. Circulating forms of intercellular adhesion molecule (ICAM)-1 in mice lacking membranous ICAM-1. Blood 95(4):1350-5. [PubMed: 10666210]  [MGI Ref ID J:110274]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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