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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1p Donating Investigator Steven Libutti, National Cancer Institute Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed only in parathyroid tissue; no activity is seen in thyroid, muscle, lymph node, trachea, thymus, salivary tissues, lung, heart, liver, brain, stomach, spleen, kidney, large intestine, small intestine, and pancreas. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in parathyroid-specific deletion of the flanked genome. These transgenic mice may be useful in generating mouse models of parathyroid-specific deletion of genes of interest, such as multiple endocrine neoplasia type 1, extracellular calcium-sensing receptor, and vitamin D receptor.Development
A targeting vector was designed to place a cre recombinase gene (followed by a metallothionein-1 polyadenylation signal) under control of the human parathyroid hormone (PTH) regulatory elements. This construct was microinjected into FVB embryos. Founder 4167 was bred to FVB. Transgenic mice were next crossed to 129SvEvTac mice carrying a loxP-flanked mutation of the Men1 gene. The resulting offspring were bred together and selected for the transgene/against the targeted mutation to obtain PTH-cre transgenic mice on a mixed 129;FVB background. This line was maintained by breeding transgenic positive mice together for an unknown number of generations prior to arriving at The Jackson Laboratory. Upon arrival, mutant mice were bred to FVB/NJ mice for one generation and are now maintained by breeding transgenic mice with wild-type siblings.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedEndocrine Deficiency Research
Research Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
Cre Recombinase Expression
Endocrine Deficiency Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(PTH-cre)4167Slib | ||
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| Allele Name | transgene insertion 4167, Steven K Libutti | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | PTH-cre; Tg(PTH-cre)1Slib; | ||
| Mutation Made By | Steven Libutti, National Cancer Institute | ||
| Site of Expression | parathyroid tissue | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | PTH, parathyroid hormone, human | ||
| Driver Note | PTH | ||
| Molecular Note | A 5' upstream regulatory region of the human PTH gene was adjoined to sequence encoding cre recombinase followed by a metallothionein 1 polyadenylation signal. The expression of cre recombinase is parathyroid cell specific. [MGI Ref ID J:86642] | ||
| Gene Symbol and Name | Tg(PTH-cre)4167Slib, transgene insertion 4167, Steven K Libutti | ||
| Chromosome | UN | ||
| Gene Common Name(s) | PTH-cre; Tg(PTH-cre)1Slib; transgene insertion 1, Steven K Libutti; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Libutti SK; Crabtree JS; Lorang D; Burns AL; Mazzanti C; Hewitt SM; O'Connor S; Ward JM; Emmert-Buck MR; Remaley A; Miller M; Turner E; Alexander HR; Arnold A; Marx SJ; Collins FS; Spiegel AM. 2003. Parathyroid gland-specific deletion of the mouse Men1 gene results in parathyroid neoplasia and hypercalcemic hyperparathyroidism. Cancer Res 63(22):8022-8. [PubMed: 14633735] [MGI Ref ID J:86642]
Additional References
Tg(PTH-cre)4167Slib relatedWettschureck N; Lee E; Libutti SK; Offermanns S; Robey PG; Spiegel AM. 2007. Parathyroid-specific double knockout of Gq and G11 alpha-subunits leads to a phenotype resembling germline knockout of the extracellular Ca2+ -sensing receptor. Mol Endocrinol 21(1):274-80. [PubMed: 16988000] [MGI Ref ID J:116986]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice are bred to wildtype siblings.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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JAX® Mice, Products & Services Conditions of Use
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