Strain Name: |
129;FVB-Tg(PTH-cre)4167Slib/J |
|---|---|
Stock Number: |
005989 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | PTH; cre; Tg(PTH-cre)4167Slib; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Steven Libutti, National Cancer Institute Strain Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed only in parathyroid tissue; no activity is seen in thyroid, muscle, lymph node, trachea, thymus, salivary tissues, lung, heart, liver, brain, stomach, spleen, kidney, large intestine, small intestine, and pancreas. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in parathyroid-specific deletion of the flanked genome. These transgenic mice may be useful in generating mouse models of parathyroid-specific deletion of genes of interest, such as multiple endocrine neoplasia type 1, extracellular calcium-sensing receptor, and vitamin D receptor.Strain Development
A targeting vector was designed to place a cre recombinase gene (followed by a metallothionein-1 polyadenylation signal) under control of the human parathyroid hormone (PTH) regulatory elements. This construct was microinjected into FVB embryos. Founder 4167 was bred to FVB. Transgenic mice were next crossed to 129SvEvTac mice carrying a loxP-flanked mutation of the Men1 gene. The resulting offspring were bred together and selected for the transgene/against the targeted mutation to obtain PTH-cre transgenic mice on a mixed 129;FVB background. This line was maintained by breeding transgenic positive mice together for an unknown number of generations prior to arriving at The Jackson Laboratory. Upon arrival, mutant mice were bred to FVB/NJ mice for one generation and are now maintained by breeding transgenic mice with wildtype siblings.
Gene & Allele Details
| Allele Symbol | Tg(PTH-cre)4167Slib | ||
|---|---|---|---|
| Allele Name | transgene insertion 4167, Steven K Libutti | ||
| Common Name(s) | PTH-cre; Tg(PTH-cre)1Slib; | ||
| Mutation Made By | Steven Libutti, National Cancer Institute | ||
| Site of Expression | parathyroid tissue | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | PTH, parathyroid hormone, human | ||
| Molecular Note | A 5' upstream regulatory region of the human PTH gene was adjoined to sequence encoding cre recombinase followed by a metallothionein 1 polyadenylation signal. The expression of cre recombinase is parathyroid cell specific. [J:86642] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice are bred to wildtype siblings. |
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Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (111 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedEndocrine Deficiency Research
Research Tools
Cardiovascular Research (Cre-lox system)
Cre-lox-System (Cre Recombinase Expression)
Endocrine Deficiency Research
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesLibutti SK; Crabtree JS; Lorang D; Burns AL; Mazzanti C; Hewitt SM; O'Connor S; Ward JM; Emmert-Buck MR; Remaley A; Miller M; Turner E; Alexander HR; Arnold A; Marx SJ; Collins FS; Spiegel AM. 2003. Parathyroid gland-specific deletion of the mouse Men1 gene results in parathyroid neoplasia and hypercalcemic hyperparathyroidism. Cancer Res 63(22):8022-8. [PubMed: 14633735] [J:86642]
Price and Supply Information
| Strain Name: | 129;FVB-Tg(PTH-cre)4167Slib/J |
| Stock Number: | 005989 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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