Strain Name:

STOCK Efna2tm1Jgf Efna5tm1Ddmo/J

Stock Number:

005992

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These ephrin A2/ephrin A5 double mutant mice may be useful in studies of retinocollicular mapping, axon topography, neuron projection, and modality-specific compartmentalization of visual, auditory, and somatosensory thalamic relay pathways.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
GenerationN1F3pN1
Generation Definitions
 
Donating Investigator David Feldhiem,   University of California at Santa Cruz

Description
Mice homozygous for both targeted mutations are viable, fertile, and normal in size. While the donating investigator reports that 10-20% of females homozygous at both loci neglect their litters, no such neglect is reported in the colonies at The Jackson Laboratory (Aug 2009). Double homozygous mice have no endogenous protein expression in inferior colliculus (IC) or superior colliculus (SC), and thus lack the concentration gradient created by the endogenous proteins across the midbrain in wildtype mice. Temporal and nasal retinal axon termination is severely altered: multiple ectopic aborizations in the SC indicate abnormalities in both anteroposterior and dorsoventral topography. Following surgical ablation of portions of the midbrain (including IC and SC), cross-modal innervation by retinal neurons is greater in double homozygous mutants compared to wildtype. Mice heterozygous at both loci are reported to have greater reproductive performance compared to double homozygous mice. Further, double heterozygotes have temporal (but not nasal) retinal axon aborization in the SC with diminished frequency and severity. These ephrin A2/ephrin A5 double mutant mice may be useful in studies of retinocollicular mapping, axon topography, neuron projection, and modality-specific compartmentalization of visual, auditory, and somatosensory thalamic relay pathways.

Development
These mice harbor two targeted mutations; ephrin A2 and ephrin A5. For the ephrin A2 targeted mutation, a targeting vector was designed to insert a neomycin cassette in exon 2 after amino acid 66 (and just upstream of a cysteine repeat motif conserved throughout the ephrin family) of the Efna2 gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Chimeric mice were bred with 129/SvEv mice. Mutant offspring were crossed to create homozygous mutant mice (Efna2-/-). The ephrin A5 targeted mutation was independently created by replacing the portion of Efna5 exon 2 encoding amino acids 42-129 with a PGK-neo cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 ES cells. Chimeric mice were bred with C57BL/6 mice. Mutant offspring were crossed to Swiss Webster mice and then made homozygous for this mutation (Efna5-/-). Double mutant mice were obtained by crossing Efna2-/- in a 129/SvEv background with a Efna5-/- in a mixed background (Swiss-Webster, C57BL/6, 129). Upon arrival at The Jackson Laboratory, double mutant mice were bred with C57BL/6J for at least one generation to establish the colony.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Efna2tm1Jgf/Efna2+ Efna5tm1Ddmo/Efna5+

        involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
  • nervous system phenotype
  • abnormal sensory neuron innervation pattern
    • single ectopic arborizations of temporal axons from the retina but normal nasal arborizations   (MGI Ref ID J:61499)

Efna2tm1Jgf/Efna2tm1Jgf Efna5tm1Ddmo/Efna5tm1Ddmo

        involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
  • nervous system phenotype
  • abnormal sensory neuron innervation pattern
    • ectopic arborization of both retinal temporal axons and nasal axons are more sever than in singly homozygous mice   (MGI Ref ID J:61499)
    • usually multiple ectopic arborizations are seen   (MGI Ref ID J:61499)
    • normal arborization sometimes lost   (MGI Ref ID J:61499)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      brain
Neurodevelopmental Defects

Neurobiology Research
Neurodevelopmental Defects

Research Tools
Neurobiology Research
Sensorineural Research

Sensorineural Research
Eye Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Efna2tm1Jgf
Allele Name targeted mutation 1, John G Flanagan
Allele Type Targeted (Null/Knockout)
Common Name(s) A2-; ephrin-A2-;
Strain of Origin129S6/SvEvTac
ES Cell Line NameTC1/TC-1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Efna2, ephrin A2
Chromosome 10
Gene Common Name(s) Cek7-L; ELF-1; EPLG6; Ephrin-A2; Epl6; HEK7-L; LERK-6; LERK6; eph-related receptor tyrosine kinase ligand 6; ephrin A6;
Molecular Note A neomycin selection cassette, containing stop codons in all three reading frames, was inserted after the sequence encoding amino acid 66. The insertion was upstream of a conserved cysteine residue motif. RT-PCR analysis of midbrain RNA showed an absenceof transcript in homozygous mutant embryos. [MGI Ref ID J:61499]
 
Allele Symbol Efna5tm1Ddmo
Allele Name targeted mutation 1, Dennis D M O'Leary
Allele Type Targeted (Null/Knockout)
Common Name(s) A5-; eA5KO; ephrin-A5-;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Efna5, ephrin A5
Chromosome 17
Gene Common Name(s) AF1; AL-1; AV158822; EFL-5; EFL5; EPLG7; Ephrin-A5; Epl7; GLC1M; LERK-7; LERK7; RAGS; eph-related receptor tyrosine kinase ligand 7; expressed sequence AV158822;
Molecular Note A neomycin selection cassette replaced sequence encoding amino acids 42 through 129 as well as a 5' splice acceptor. RT-PCR analysis showed an absence of transcript in homozygous mutant mice. [MGI Ref ID J:45896]

Genotyping

Genotyping Information

Genotyping Protocols

Efna2tm1Jgf, Standard PCR
Efna5tm1Jgf, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Feldheim DA; Kim YI; Bergemann AD; Frisen J; Barbacid M; Flanagan JG. 2000. Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping [see comments] Neuron 25(3):563-74. [PubMed: 10774725]  [MGI Ref ID J:61499]

Additional References

Efna2tm1Jgf related

Bonanomi D; Chivatakarn O; Bai G; Abdesselem H; Lettieri K; Marquardt T; Pierchala BA; Pfaff SL. 2012. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell 148(3):568-82. [PubMed: 22304922]  [MGI Ref ID J:180801]

Brennaman LH; Zhang X; Guan H; Triplett JW; Brown A; Demyanenko GP; Manis PB; Landmesser L; Maness PF. 2013. Polysialylated NCAM and ephrinA/EphA regulate synaptic development of GABAergic interneurons in prefrontal cortex. Cereb Cortex 23(1):162-77. [PubMed: 22275477]  [MGI Ref ID J:204427]

Cang J; Kaneko M; Yamada J; Woods G; Stryker MP; Feldheim DA. 2005. Ephrin-as guide the formation of functional maps in the visual cortex. Neuron 48(4):577-89. [PubMed: 16301175]  [MGI Ref ID J:107593]

Cang J; Niell CM; Liu X; Pfeiffenberger C; Feldheim DA; Stryker MP. 2008. Selective disruption of one Cartesian axis of cortical maps and receptive fields by deficiency in ephrin-As and structured activity. Neuron 57(4):511-23. [PubMed: 18304481]  [MGI Ref ID J:132879]

Cang J; Wang L; Stryker MP; Feldheim DA. 2008. Roles of ephrin-as and structured activity in the development of functional maps in the superior colliculus. J Neurosci 28(43):11015-23. [PubMed: 18945909]  [MGI Ref ID J:143794]

Chadaram SR; Laskowski MB; Madison RD. 2007. Topographic specificity within membranes of a single muscle detected in vitro. J Neurosci 27(51):13938-48. [PubMed: 18094231]  [MGI Ref ID J:141637]

Ellsworth CA; Lyckman AW; Feldheim DA; Flanagan JG; Sur M. 2005. Ephrin-A2 and -A5 influence patterning of normal and novel retinal projections to the thalamus: conserved mapping mechanisms in visual and auditory thalamic targets. J Comp Neurol 488(2):140-51. [PubMed: 15924339]  [MGI Ref ID J:99706]

Feng G; Laskowski MB; Feldheim DA; Wang H; Lewis R; Frisen J; Flanagan JG; Sanes JR. 2000. Roles for ephrins in positionally selective synaptogenesis between motor neurons and muscle fibers. Neuron 25(2):295-306. [PubMed: 10719886]  [MGI Ref ID J:60774]

Haustead DJ; Lukehurst SS; Clutton GT; Bartlett CA; Dunlop SA; Arrese CA; Sherrard RM; Rodger J. 2008. Functional topography and integration of the contralateral and ipsilateral retinocollicular projections of ephrin-A-/- mice. J Neurosci 28(29):7376-86. [PubMed: 18632942]  [MGI Ref ID J:139255]

Holmberg J; Armulik A; Senti KA; Edoff K; Spalding K; Momma S; Cassidy R; Flanagan JG; Frisen J. 2005. Ephrin-A2 reverse signaling negatively regulates neural progenitor proliferation and neurogenesis. Genes Dev 19(4):462-71. [PubMed: 15713841]  [MGI Ref ID J:96025]

Jiao JW; Feldheim DA; Chen DF. 2008. Ephrins as negative regulators of adult neurogenesis in diverse regions of the central nervous system. Proc Natl Acad Sci U S A 105(25):8778-83. [PubMed: 18562299]  [MGI Ref ID J:137200]

Lyckman AW; Jhaveri S; Feldheim DA; Vanderhaeghen P; Flanagan JG; Sur M. 2001. Enhanced plasticity of retinothalamic projections in an ephrin-A2/A5 double mutant. J Neurosci 21(19):7684-90. [PubMed: 11567058]  [MGI Ref ID J:71649]

Makowiecki K; Hammond G; Rodger J. 2012. Different levels of food restriction reveal genotype-specific differences in learning a visual discrimination task. PLoS One 7(11):e48703. [PubMed: 23144936]  [MGI Ref ID J:195031]

Park E; Kim Y; Noh H; Lee H; Yoo S; Park S. 2013. EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development. Cell Death Differ 20(1):169-80. [PubMed: 22976838]  [MGI Ref ID J:205629]

Pfeiffenberger C; Cutforth T; Woods G; Yamada J; Renteria RC; Copenhagen DR; Flanagan JG; Feldheim DA. 2005. Ephrin-As and neural activity are required for eye-specific patterning during retinogeniculate mapping. Nat Neurosci 8(8):1022-7. [PubMed: 16025107]  [MGI Ref ID J:101436]

Pfeiffenberger C; Yamada J; Feldheim DA. 2006. Ephrin-As and patterned retinal activity act together in the development of topographic maps in the primary visual system. J Neurosci 26(50):12873-84. [PubMed: 17167078]  [MGI Ref ID J:116767]

Ting MC; Wu NL; Roybal PG; Sun J; Liu L; Yen Y; Maxson RE Jr. 2009. EphA4 as an effector of Twist1 in the guidance of osteogenic precursor cells during calvarial bone growth and in craniosynostosis. Development 136(5):855-64. [PubMed: 19201948]  [MGI Ref ID J:146459]

Torii M; Hashimoto-Torii K; Levitt P; Rakic P. 2009. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature 461(7263):524-8. [PubMed: 19759535]  [MGI Ref ID J:153504]

Triplett JW; Phan A; Yamada J; Feldheim DA. 2012. Alignment of multimodal sensory input in the superior colliculus through a gradient-matching mechanism. J Neurosci 32(15):5264-71. [PubMed: 22496572]  [MGI Ref ID J:184449]

Wang L; Klein R; Zheng B; Marquardt T. 2011. Anatomical Coupling of Sensory and Motor Nerve Trajectory via Axon Tracking. Neuron 71(2):263-77. [PubMed: 21791286]  [MGI Ref ID J:174684]

Wilks TA; Rodger J; Harvey AR. 2010. A role for ephrin-As in maintaining topographic organization in register across interconnected central visual pathways. Eur J Neurosci 31(4):613-22. [PubMed: 20384808]  [MGI Ref ID J:159859]

Yu X; Wang G; Gilmore A; Yee AX; Li X; Xu T; Smith SJ; Chen L; Zuo Y. 2013. Accelerated experience-dependent pruning of cortical synapses in ephrin-A2 knockout mice. Neuron 80(1):64-71. [PubMed: 24094103]  [MGI Ref ID J:201801]

Efna5tm1Ddmo related

Bonanomi D; Chivatakarn O; Bai G; Abdesselem H; Lettieri K; Marquardt T; Pierchala BA; Pfaff SL. 2012. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell 148(3):568-82. [PubMed: 22304922]  [MGI Ref ID J:180801]

Brennaman LH; Zhang X; Guan H; Triplett JW; Brown A; Demyanenko GP; Manis PB; Landmesser L; Maness PF. 2013. Polysialylated NCAM and ephrinA/EphA regulate synaptic development of GABAergic interneurons in prefrontal cortex. Cereb Cortex 23(1):162-77. [PubMed: 22275477]  [MGI Ref ID J:204427]

Cang J; Kaneko M; Yamada J; Woods G; Stryker MP; Feldheim DA. 2005. Ephrin-as guide the formation of functional maps in the visual cortex. Neuron 48(4):577-89. [PubMed: 16301175]  [MGI Ref ID J:107593]

Cang J; Niell CM; Liu X; Pfeiffenberger C; Feldheim DA; Stryker MP. 2008. Selective disruption of one Cartesian axis of cortical maps and receptive fields by deficiency in ephrin-As and structured activity. Neuron 57(4):511-23. [PubMed: 18304481]  [MGI Ref ID J:132879]

Cang J; Wang L; Stryker MP; Feldheim DA. 2008. Roles of ephrin-as and structured activity in the development of functional maps in the superior colliculus. J Neurosci 28(43):11015-23. [PubMed: 18945909]  [MGI Ref ID J:143794]

Chadaram SR; Laskowski MB; Madison RD. 2007. Topographic specificity within membranes of a single muscle detected in vitro. J Neurosci 27(51):13938-48. [PubMed: 18094231]  [MGI Ref ID J:141637]

Cooper MA; Son AI; Komlos D; Sun Y; Kleiman NJ; Zhou R. 2008. Loss of ephrin-A5 function disrupts lens fiber cell packing and leads to cataract. Proc Natl Acad Sci U S A 105(43):16620-5. [PubMed: 18948590]  [MGI Ref ID J:144621]

Cutforth T; Moring L; Mendelsohn M; Nemes A; Shah NM; Kim MM; Frisen J; Axel R. 2003. Axonal ephrin-As and odorant receptors: coordinate determination of the olfactory sensory map. Cell 114(3):311-22. [PubMed: 12914696]  [MGI Ref ID J:101720]

Depaepe V; Suarez-Gonzalez N; Dufour A; Passante L; Gorski JA; Jones KR; Ledent C; Vanderhaeghen P. 2005. Ephrin signalling controls brain size by regulating apoptosis of neural progenitors. Nature 435(7046):1244-50. [PubMed: 15902206]  [MGI Ref ID J:99355]

Deschamps C; Faideau M; Jaber M; Gaillard A; Prestoz L. 2009. Expression of ephrinA5 during development and potential involvement in the guidance of the mesostriatal pathway. Exp Neurol 219(2):466-80. [PubMed: 19576892]  [MGI Ref ID J:154889]

Dufour A; Seibt J; Passante L; Depaepe V; Ciossek T; Frisen J; Kullander K; Flanagan JG; Polleux F; Vanderhaeghen P. 2003. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes. Neuron 39(3):453-65. [PubMed: 12895420]  [MGI Ref ID J:85411]

Ellsworth CA; Lyckman AW; Feldheim DA; Flanagan JG; Sur M. 2005. Ephrin-A2 and -A5 influence patterning of normal and novel retinal projections to the thalamus: conserved mapping mechanisms in visual and auditory thalamic targets. J Comp Neurol 488(2):140-51. [PubMed: 15924339]  [MGI Ref ID J:99706]

Feng G; Laskowski MB; Feldheim DA; Wang H; Lewis R; Frisen J; Flanagan JG; Sanes JR. 2000. Roles for ephrins in positionally selective synaptogenesis between motor neurons and muscle fibers. Neuron 25(2):295-306. [PubMed: 10719886]  [MGI Ref ID J:60774]

Frisen J; Yates PA; McLaughlin T; Friedman GC; O'Leary DD; Barbacid M. 1998. Ephrin-A5 (AL-1/RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system. Neuron 20(2):235-43. [PubMed: 9491985]  [MGI Ref ID J:45896]

Hara Y; Nomura T; Yoshizaki K; Frisen J; Osumi N. 2010. Impaired hippocampal neurogenesis and vascular formation in ephrin-A5-deficient mice. Stem Cells 28(5):974-83. [PubMed: 20474079]  [MGI Ref ID J:168862]

Haustead DJ; Lukehurst SS; Clutton GT; Bartlett CA; Dunlop SA; Arrese CA; Sherrard RM; Rodger J. 2008. Functional topography and integration of the contralateral and ipsilateral retinocollicular projections of ephrin-A-/- mice. J Neurosci 28(29):7376-86. [PubMed: 18632942]  [MGI Ref ID J:139255]

Holmberg J; Clarke DL; Frisen J. 2000. Regulation of repulsion versus adhesion by different splice forms of an Eph receptor. Nature 408(6809):203-6. [PubMed: 11089974]  [MGI Ref ID J:77779]

Lehigh KM; Leonard CE; Baranoski J; Donoghue MJ. 2013. Parcellation of the thalamus into distinct nuclei reflects EphA expression and function. Gene Expr Patterns 13(8):454-63. [PubMed: 24036135]  [MGI Ref ID J:202990]

Lyckman AW; Jhaveri S; Feldheim DA; Vanderhaeghen P; Flanagan JG; Sur M. 2001. Enhanced plasticity of retinothalamic projections in an ephrin-A2/A5 double mutant. J Neurosci 21(19):7684-90. [PubMed: 11567058]  [MGI Ref ID J:71649]

Otal R; Burgaya F; Frisen J; Soriano E; Martinez A. 2006. Ephrin-A5 modulates the topographic mapping and connectivity of commissural axons in murine hippocampus. Neuroscience 141(1):109-21. [PubMed: 16690216]  [MGI Ref ID J:110251]

Passante L; Gaspard N; Degraeve M; Frisen J; Kullander K; De Maertelaer V; Vanderhaeghen P. 2008. Temporal regulation of ephrin/Eph signalling is required for the spatial patterning of the mammalian striatum. Development 135(19):3281-90. [PubMed: 18755772]  [MGI Ref ID J:138785]

Pfeiffenberger C; Cutforth T; Woods G; Yamada J; Renteria RC; Copenhagen DR; Flanagan JG; Feldheim DA. 2005. Ephrin-As and neural activity are required for eye-specific patterning during retinogeniculate mapping. Nat Neurosci 8(8):1022-7. [PubMed: 16025107]  [MGI Ref ID J:101436]

Pfeiffenberger C; Yamada J; Feldheim DA. 2006. Ephrin-As and patterned retinal activity act together in the development of topographic maps in the primary visual system. J Neurosci 26(50):12873-84. [PubMed: 17167078]  [MGI Ref ID J:116767]

Prakash N; Vanderhaeghen P; Cohen-Cory S; Frisen J; Flanagan JG; Frostig RD. 2000. Malformation of the functional organization of somatosensory cortex in adult ephrin-A5 knock-out mice revealed by in vivo functional imaging. J Neurosci 20(15):5841-7. [PubMed: 10908626]  [MGI Ref ID J:63611]

Sheleg M; Yochum CL; Wagner GC; Zhou R; Richardson JR. 2013. Ephrin-A5 deficiency alters sensorimotor and monoaminergic development. Behav Brain Res 236(1):139-47. [PubMed: 22954718]  [MGI Ref ID J:193948]

Son AI; Cooper MA; Sheleg M; Sun Y; Kleiman NJ; Zhou R. 2013. Further analysis of the lens of ephrin-A5-/- mice: development of postnatal defects. Mol Vis 19:254-66. [PubMed: 23401654]  [MGI Ref ID J:195994]

Torii M; Hashimoto-Torii K; Levitt P; Rakic P. 2009. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature 461(7263):524-8. [PubMed: 19759535]  [MGI Ref ID J:153504]

Triplett JW; Phan A; Yamada J; Feldheim DA. 2012. Alignment of multimodal sensory input in the superior colliculus through a gradient-matching mechanism. J Neurosci 32(15):5264-71. [PubMed: 22496572]  [MGI Ref ID J:184449]

Wang L; Klein R; Zheng B; Marquardt T. 2011. Anatomical Coupling of Sensory and Motor Nerve Trajectory via Axon Tracking. Neuron 71(2):263-77. [PubMed: 21791286]  [MGI Ref ID J:174684]

Wilks TA; Rodger J; Harvey AR. 2010. A role for ephrin-As in maintaining topographic organization in register across interconnected central visual pathways. Eur J Neurosci 31(4):613-22. [PubMed: 20384808]  [MGI Ref ID J:159859]

Yabuta NH; Butler AK; Callaway EM. 2000. Laminar specificity of local circuits in barrel cortex of ephrin-A5 knockout mice. J Neurosci 20(15):RC88. [PubMed: 10899175]  [MGI Ref ID J:70429]

Yumoto N; Wakatsuki S; Kurisaki T; Hara Y; Osumi N; Frisen J; Sehara-Fujisawa A. 2008. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction. PLoS ONE 3(10):e3322. [PubMed: 18830404]  [MGI Ref ID J:144429]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, mice that are homozygous for the Efna2tm1Jgf and heterozygous for the Efna5tm1Ddmo mutations are bred to mice that are heterozygous for the Efna2tm1Jgf and homozygous for the Efna5tm1Ddmo mutation. The donating investigator reports that 10-20% of females homozygous at both loci neglect their litters.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

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  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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