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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6(SJL)-Tg(SBE/Tk-luc)7Twc/J (Changed: 05-SEP-11 ) B6.FVB-Tg(SBE/Tk-luc)7Twc/J (Changed: 24-MAY-06 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 08-NOV-07 Species laboratory mouse Generation N10+N1F17 (26-DEC-12)
Generation DefinitionsDonating Investigator Tony Wyss-Coray, Stanford University Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express luciferase in response to activation of the Smad2/3-dependent signaling pathway. Cultured primary astrocytes isolated from transgenic mice exhibited luciferase activity when stimulated with TGF-beta. Higher treatment levels of activin and nodal elicited similar luciferase activity. Lipopolysaccharide (LPS) challenge results in strong bioluminescence emissions from the intestinal region and brain. Mechanical injury to the neocortex results in an increase of bioluminescence in 2 hours, which peaks at 4 hours and returns to baseline approximately 48 hours after the injury. Biochemical assays for luciferase activity correlated with noninvasive bioluminescence imaging analysis. The strain was backcrossed to the albino C57BL/6J-Tyrc-2J/J strain for 2 generations to facilitate bioluminescence imaging.Development
A transgenic construct containing the firefly luciferase gene under the control of 12 Smad-binding element repeats, herpes simplex virus thymidine kinase minimal promoter and an SV40 polyadenylation signal was injected into fertilized B6SJLF1 eggs. The donating investigator reported that founder line 7 (T9-7B) animals were backcrossed onto C57BL/6J for 8 generations and then backcrossed onto C57BL/6J-Tyrc-2J/J for 2 generations.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(SBE/TK-luc)7Twc/0
either: (involves: FVB/N) or (involves: C57BL/6 * SJL)
- normal phenotype
- no abnormal phenotype detected
- mice do not display abnormalities resulting from transgene expression (MGI Ref ID J:105769)
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Immunology and Inflammation Research
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(SBE/TK-luc)7Twc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Tony Wyss-Coray | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | SBE-luc; T9-7; T9-7BA; | ||
| Mutation Made By | Tony Wyss-Coray, Stanford University | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | Luciferase is expressed in response to activation of the Smad2/3-dependent signaling pathway. Baseline activity is highest in brain, intestine, heart, and skin. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | HSV-TK, herpes simplex virus thymidine kinase, | ||
| General Note | Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express luciferase in response to activation of the Smad2/3-dependent signaling pathway. Cultured primary astrocytes isolated from transgenic mice exhibited luciferase activity when stimulated with TGF-beta. Higher treatment levels of activin and nodal elicited similar luciferase activity. Lipopolysaccharide (LPS) challenge results in strong bioluminescence emissions from the intestinal region and brain. Mechanical injury to the neocortex results in an increase of bioluminescence in 2 hours, which peaks at 4 hours and returns to baseline approximately 48 hours after the injury. Biochemical assays for luciferase activity correlated with noninvasive bioluminescence imaging analysis. This mutant mouse strain may be useful in studies of the role of transforming growth factor beta in intracellular signaling with noninvasive in vivo imaging. | ||
| Molecular Note | This transgene expresses the firefly luciferase gene under the control of 12 Smad-binding element repeats, herpes simplex virus thymidine kinase minimal promoter and an SV40 polyadenylation signal. Luciferase is expressed in response to activation of the Smad2/3-dependent signaling pathway. Lines T9-7, T9-55, T9-90, and T9-98 were generated. [MGI Ref ID J:105769] | ||
| Gene Symbol and Name | Tg(SBE/TK-luc)7Twc, transgene insertion 1, Tony Wyss-Coray | ||
| Chromosome | UN | ||
| Gene Common Name(s) | SBE-luc; T9-7; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(SBE/Tk-luc)7Twc, QPCR
Tg(SBE/Tk-luc)7Twc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lin AH; Luo J; Mondshein LH; ten Dijke P; Contag CH; Wyss-Coray T. 2005. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury J Immunol 175(1):547-54. [PubMed: 15972691] [MGI Ref ID J:105769]
Additional References
Tg(SBE/TK-luc)7Twc relatedGiroux M; Delisle JS; O'Brien A; Hebert MJ; Perreault C. 2010. T cell activation leads to protein kinase Ctheta-dependent inhibition of TGF-beta signaling. J Immunol 185(3):1568-76. [PubMed: 20592275] [MGI Ref ID J:162461]
Luo J; Ho PP; Buckwalter MS; Hsu T; Lee LY; Zhang H; Kim DK; Kim SJ; Gambhir SS; Steinman L; Wyss-Coray T. 2007. Glia-dependent TGF-beta signaling, acting independently of the TH17 pathway, is critical for initiation of murine autoimmune encephalomyelitis. J Clin Invest 117(11):3306-15. [PubMed: 17965773] [MGI Ref ID J:127382]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 08-NOV-07 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(SBE/TK-luc)7Twc
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(SBE/TK-luc)7Twc x Homozygous for Tg(SBE/TK-luc)7Twc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(SBE/TK-luc)7Twc
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(SBE/TK-luc)7Twc x Homozygous for Tg(SBE/TK-luc)7Twc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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