Strain Name: |
B6(SJL)-Tg(SBE/Tk-luc)7Twc/J |
|---|---|
Stock Number: |
005999 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.FVB-Tg(SBE/Tk-luc)7Twc/J (Changed: 24-MAY-06
) |
| Genes & Alleles | luc; Tg(SBE/Tk-luc)7Twc; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Tony Wyss-Coray, Stanford University Generation N10+N1F4 (28-DEC-07) Strain Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express luciferase in response to activation of the Smad2/3-dependent signaling pathway. Cultured primary astrocytes isolated from transgenic mice exhibited luciferase activity when stimulated with TGF-beta. Higher treatment levels of activin and nodal elicited similar luciferase activity. Lipopolysaccharide (LPS) challenge results in strong bioluminescence emissions from the intestinal region and brain. Mechanical injury to the neocortex results in an increase of bioluminescence in 2 hours, which peaks at 4 hours and returns to baseline approximately 48 hours after the injury. Biochemical assays for luciferase activity correlated with noninvasive bioluminescence imaging analysis. The strain was backcrossed to the albino C57BL/6J-Tyrc-2J/J strain for 2 generations to facilitate bioluminescence imaging. This mutant mouse strain may be useful in studies of the role of transforming growth factor beta in intracellular signaling with noninvasive in vivo imaging.Strain Development
A transgenic construct containing the firefly luciferase gene under the control of 12 Smad-binding element repeats, herpes simplex virus thymidine kinase minimal promoter and an SV40 polyadenylation signal was injected into fertilized B6SJLF1 eggs. Founder line 7 (T9-7B) animals were backcrossed onto C57BL/6J for 8 generations and then backcrossed onto C57BL/6J-Tyrc-2J/J for 2 generations.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(SBE/Tk-luc)7Twc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Tony Wyss-Coray | ||
| Common Name(s) | SBE-luc; T9-7; | ||
| Mutation Made By | Tony Wyss-Coray, Stanford University | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Expressed Gene | luc, luciferase, firefly | ||
| General Note | Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express luciferase in response to activation of the Smad2/3-dependent signaling pathway. Cultured primary astrocytes isolated from transgenic mice exhibited luciferase activity when stimulated with TGF-beta. Higher treatment levels of activin and nodal elicited similar luciferase activity. Lipopolysaccharide (LPS) challenge results in strong bioluminescence emissions from the intestinal region and brain. Mechanical injury to the neocortex results in an increase of bioluminescence in 2 hours, which peaks at 4 hours and returns to baseline approximately 48 hours after the injury. Biochemical assays for luciferase activity correlated with noninvasive bioluminescence imaging analysis. This mutant mouse strain may be useful in studies of the role of transforming growth factor beta in intracellular signaling with noninvasive in vivo imaging. | ||
| Molecular Note | This transgene expresses the firefly luciferase gene under the control of 12 Smad-binding element repeats, herpes simplex virus thymidine kinase minimal promoter and an SV40 polyadenylation signal. Luciferase is expressed in response to activation of the Smad2/3-dependent signaling pathway. [J:105769] | ||
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Tg(SBE/Tk-luc)7Twc
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Fluorescent Protein Strains
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006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J 006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J View Strains carrying other alleles of luc (9 strains)
Fluorescent Proteins/lacZ Systems
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Selected Reference(s)
Additional ReferencesLin AH; Luo J; Mondshein LH; ten Dijke P; Contag CH; Wyss-Coray T. 2005. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury J Immunol 175(1):547-54. [PubMed: 15972691] [J:105769]
| Strain Name: | B6(SJL)-Tg(SBE/Tk-luc)7Twc/J |
| Stock Number: | 005999 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of these Strains by companies or for-profit entities requires a license from J. David Gladstone Institutes.
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