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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.FVB-Tg(SBE/Tk-luc)7Twc/J (Changed: 24-MAY-06 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 08-NOV-07 Species laboratory mouse Generation N10+N1F10 (04-NOV-09) Donating Investigator Tony Wyss-Coray, Stanford University Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express luciferase in response to activation of the Smad2/3-dependent signaling pathway. Cultured primary astrocytes isolated from transgenic mice exhibited luciferase activity when stimulated with TGF-beta. Higher treatment levels of activin and nodal elicited similar luciferase activity. Lipopolysaccharide (LPS) challenge results in strong bioluminescence emissions from the intestinal region and brain. Mechanical injury to the neocortex results in an increase of bioluminescence in 2 hours, which peaks at 4 hours and returns to baseline approximately 48 hours after the injury. Biochemical assays for luciferase activity correlated with noninvasive bioluminescence imaging analysis. The strain was backcrossed to the albino C57BL/6J-Tyrc-2J/J strain for 2 generations to facilitate bioluminescence imaging. This mutant mouse strain may be useful in studies of the role of transforming growth factor beta in intracellular signaling with noninvasive in vivo imaging.Development
A transgenic construct containing the firefly luciferase gene under the control of 12 Smad-binding element repeats, herpes simplex virus thymidine kinase minimal promoter and an SV40 polyadenylation signal was injected into fertilized B6SJLF1 eggs. Founder line 7 (T9-7B) animals were backcrossed onto C57BL/6J for 8 generations and then backcrossed onto C57BL/6J-Tyrc-2J/J for 2 generations.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(SBE/Tk-luc)7Twc/0
either: (involves: FVB/N) or (involves: C57BL/6 * SJL)
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:105769)
- mice do not display abnormalities resulting from transgene expression
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
Research Tools
Fluorescent Proteins
Immunology and Inflammation Research
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(SBE/Tk-luc)7Twc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Tony Wyss-Coray | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | SBE-luc; T9-7; | ||
| Mutation Made By | Tony Wyss-Coray, Stanford University | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | Luciferase is expressed in response to activation of the Smad2/3-dependent signaling pathway. Baseline activity is highest in brain, intestine, heart, and skin. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| General Note | Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express luciferase in response to activation of the Smad2/3-dependent signaling pathway. Cultured primary astrocytes isolated from transgenic mice exhibited luciferase activity when stimulated with TGF-beta. Higher treatment levels of activin and nodal elicited similar luciferase activity. Lipopolysaccharide (LPS) challenge results in strong bioluminescence emissions from the intestinal region and brain. Mechanical injury to the neocortex results in an increase of bioluminescence in 2 hours, which peaks at 4 hours and returns to baseline approximately 48 hours after the injury. Biochemical assays for luciferase activity correlated with noninvasive bioluminescence imaging analysis. This mutant mouse strain may be useful in studies of the role of transforming growth factor beta in intracellular signaling with noninvasive in vivo imaging. | ||
| Molecular Note | This transgene expresses the firefly luciferase gene under the control of 12 Smad-binding element repeats, herpes simplex virus thymidine kinase minimal promoter and an SV40 polyadenylation signal. Luciferase is expressed in response to activation of the Smad2/3-dependent signaling pathway. [MGI Ref ID J:105769] | ||
| Gene Symbol and Name | Tg(SBE/Tk-luc)7Twc, transgene insertion 1, Tony Wyss-Coray | ||
| Chromosome | UN | ||
| Gene Common Name(s) | SBE-luc; T9-7; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(SBE/Tk-luc)7Twc, QPCR
Tg(SBE/Tk-luc)7Twc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Lin AH; Luo J; Mondshein LH; ten Dijke P; Contag CH; Wyss-Coray T. 2005. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury J Immunol 175(1):547-54. [PubMed: 15972691] [MGI Ref ID J:105769]
Additional References
Tg(SBE/Tk-luc)7Twc relatedLuo J; Ho PP; Buckwalter MS; Hsu T; Lee LY; Zhang H; Kim DK; Kim SJ; Gambhir SS; Steinman L; Wyss-Coray T. 2007. Glia-dependent TGF-beta signaling, acting independently of the TH17 pathway, is critical for initiation of murine autoimmune encephalomyelitis. J Clin Invest 117(11):3306-15. [PubMed: 17965773] [MGI Ref ID J:127382]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 08-NOV-07 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(SBE/Tk-luc)7Twc
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(SBE/Tk-luc)7Twc x Homozygous for Tg(SBE/Tk-luc)7Twc
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(SBE/Tk-luc)7Twc
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(SBE/Tk-luc)7Twc x Homozygous for Tg(SBE/Tk-luc)7Twc
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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