Strain Name: |
B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J |
|---|---|
Stock Number: |
006000 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; ITGAM; DTR; Tg(ITGAM-DTR/EGFP)34Lan; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Richard Lang, Cincinnati Children's Hospital Generation N6+N4F1 (12-MAY-08) Strain Description
These transgenic mice have a diphtheria toxin (DT) inducible system that transiently depletes macrophages. The transgene insert contains a fusion product involving simian diphtheria toxin receptor and green fluorescent protein under the control of the human ITGAM (integrin alpha M) promoter (CD11b). RT-PCR analysis of bone marrow macrophages detects specific transgene expression. Cytological analysis of thioglycollate treated peritoneal cells shows the absence of macrophages. Intraperitoneal injection of DT ablates monocyte/macrophage cells in the peritoneal cavity. Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgene expression is not sufficient to be detected by FACS analysis. 12 hours following DT administration all macrophages are ablated in the peritoneum, kidney and ovary. Macrophage population is restored by day 4 following a single intraperitoneal dose of DT. Administration of DT does not affect polymorphonuclear cells. Higher doses (greater than 25ng/g body weight) of DT sickens the mice. This mutant mouse strain may be useful in studies of the specific role of macrophages in the immune response.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing sequence encoding DTR/GFP under the control of the human ITGAM (integrin alpha M) promoter was introduced into fertilized FVB/N donor eggs. Founder line 34 was subsequently established. The mice were backcrossed onto C57BL/6 for 6 generations (January 2006).
Gene & Allele Details
| Allele Symbol | Tg(ITGAM-DTR/EGFP)34Lan | ||
|---|---|---|---|
| Allele Name | transgene insertion 34, Richard A Lang | ||
| Common Name(s) | CD11b-DTR; | ||
| Mutation Made By | Richard Lang, Cincinnati Children's Hospital | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Induced in monocytes/macrophages (fluorescence not sufficient for FACS); transient diptheria toxin (DT)-induced ablation of cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | DTR, Simian Diphtheria Toxin Receptor, | ||
| Promoter | ITGAM, integrin, alpha M (complement component 3 receptor 3 subunit), human | ||
| General Note | Mice carrying this transgene have a diphtheria toxin (DT) inducible system that transiently depletes macrophages. The transgene contains a fusion product involving simian diphtheria toxin receptor and green fluorescent protein under the control of the human ITGAM (integrin alpha M) promoter (CD11b), and human growth hormone sequence. RT-PCR analysis of bone marrow macrophages detects specific transgene expression. Cytological analysis of thioglycollate treated peritoneal cells shows the absence of macrophages. Intraperitoneal injection of DT ablates monocyte/macrophage cells in the peritoneal cavity. | ||
| Molecular Note | The transgene contains sequence encoding DTR/GFP under the control of the human ITGAM (integrin alpha M) promoter. [J:95141] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Itgax-DTR/GFP
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous mice are bred together. If generated, homozygous offspring can be bred together, although the phenotype of such animals is not currently known. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
005515 FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
004509 B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J 004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cell Biology Research
Fluorescent Proteins
Hematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesDuffield JS; Forbes SJ; Constandinou CM; Clay S; Partolina M; Vuthoori S; Wu S; Lang R; Iredale JP. 2005. Selective depletion of macrophages reveals distinct, opposing roles during liver injury and repair. J Clin Invest 115(1):56-65. [PubMed: 15630444] [J:95141]
Price and Supply Information
| Strain Name: | B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J |
| Stock Number: | 006000 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Homozygotes are not available for this strain. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of this Strain by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form