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| This strain is now distributed by the Mutant Mouse Regional Resource Center. Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for ordering information and strain details on B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/Mmjax
MMRRC Stock Number 034835. As a designated MMRRC center, The Jackson Laboratory will continue to distribute these mice at the same high health and quality standards but ordering is exclusively provided through the MMRRC. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J (Changed: 11-AUG-11 ) B6.Cg-Tg(APPswe)E1-2Dbo/J (Changed: 13-FEB-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N15p
Generation DefinitionsDonating Investigator Dr. David R. Borchelt, McKnight Brain Inst, Univ of Florida Description
These transgenic mice express a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter. Mice that are hemizygous for the transgene are viable and fertile. More than half of the female hemizygous mice do not survive past 15 months of age. This mutant mouse strain may be useful in studies of Alzheimer's Disease.Development
A transgenic construct a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter was injected into fertilized C57BL/6J X C3HeJ F2 mouse eggs. Founder line E1-2 was established. The mice were then backcrossed to C57BL/6J for more than 14 generations.
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (107 strains)
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Alzheimer Disease; AD
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Cerebral Amyloid Angiopathy, App-Related (APP)
assigned by genotype
Tg(Prnp-App/APPswe)E1-2Dbo/0
B6.C3-Tg(Prnp-App/APPswe)E1-2Dbo
- nervous system phenotype
- amyloid beta deposits
- 14-15 month old mutants backcrossed 5- or 10 generations tend to have higher accumulation of amyloid compared to Tg(APP695)3Dbo at that age (MGI Ref ID J:109847)
- behavior/neurological phenotype
- abnormal locomotor behavior
- in radial maze testing, male mutants backcrossed 5-7 generations show high motor reactivity compared to females or control animals, and entered the closest arm of the maze which prevented them from learning the task (MGI Ref ID J:109847)
- decreased vertical activity
- at 12-13 months, mice backcrossed 5-7 generations show fewer rearing reactions compared to nontransgenic littermates (MGI Ref ID J:109847)
- hypoactivity
- at 12-13 months, males backcrossed 5-7 generations are less active than controls in inner cells of open field (MGI Ref ID J:109847)
- abnormal object recognition memory
- as females age (13-14 months of age), significant difference compared to female controls is observed in trials with longest (<60 minute) delays (MGI Ref ID J:109847)
- abnormal spatial learning
- 12-13 month old males and females backcrossed 5-7 generations show poorer performance in probe trials in the Morris water maze; females spent less time in correct quadrant until fourth consectutive session (MGI Ref ID J:109847)
- upon repeat testing of the same females one month later, difference between mutants and controls is more robust; repeated testing had no effect on performance (MGI Ref ID J:109847)
- increased anxiety-related response
- at 12-13 months, females backcrossed 5-7 generations are less active in visiting open arms in plus maze; combined with reduced rearing reactions, females may have higher anxiety levels (MGI Ref ID J:109847)
- other phenotype
- amyloid beta deposits
- 14-15 month old mutants backcrossed 5- or 10 generations tend to have higher accumulation of amyloid compared to Tg(APP695)3Dbo at that age (MGI Ref ID J:109847)
This mouse can be used to support research in many areas including:
APP relatedNeurobiology Research
Alzheimer's Disease
strains expressing mutant APP
Neurobiology Research
Alzheimer's Disease
Neurodegeneration
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Prnp-App/APPswe)E1-2Dbo | ||
|---|---|---|---|
| Allele Name | transgene insertion E1-2, David R Borchelt | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | E1-2 line; Mo/Hu APPswe; Mo/Hu APPswe line E1-2; line E1-2; | ||
| Strain of Origin | (C57BL/6J x C3H/HeJ)F2 | ||
| Expressed Gene | APP, amyloid beta (A4) precursor protein, human | ||
| Promoter | Prnp, prion protein, mouse, laboratory | ||
| General Note | Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 and C3-3 (Tg(APP695)3Dbo). They were estimated, on the basis of Southern blot analysis, to carry 3-10 copies of the transgene. | ||
| Molecular Note | This transgene expresses a modified mouse amyloid precursor protein cDNA encoding the 695-amino acid isoform with a "humanized" Abeta domain amino acid sequence that includes the familial Alzheimer disease- (FAD-) associated Swedish double mutation (Lys595Asn and Met596Leu). The cDNA was inserted into an expression cassette containing the promoter, 5' intronic and 3' untranslated region sequences of the mouse prion protein gene. [MGI Ref ID J:80782] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Tg(APP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Savonenko AV; Xu GM; Price DL; Borchelt DR; Markowska AL. 2003. Normal cognitive behavior in two distinct congenic lines of transgenic mice hyperexpressing mutant APP SWE. Neurobiol Dis 12(3):194-211. [PubMed: 12742740] [MGI Ref ID J:109847]
Additional References
Tg(Prnp-App/APPswe)E1-2Dbo relatedBorchelt DR; Davis J; Fischer M; Lee MK; Slunt HH; Ratovitsky T; Regard J; Copeland NG; Jenkins NA; Sisodia SS; Price DL. 1996. A vector for expressing foreign genes in the brains and hearts of transgenic mice. Genet Anal 13(6):159-63. [PubMed: 9117892] [MGI Ref ID J:80782]
Hou Y; Aboukhatwa MA; Lei DL; Manaye K; Khan I; Luo Y. 2010. Anti-depressant natural flavonols modulate BDNF and beta amyloid in neurons and hippocampus of double TgAD mice. Neuropharmacology 58(6):911-20. [PubMed: 19917299] [MGI Ref ID J:179562]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
This strain is currently Transferred.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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