Strain Name: |
B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J |
|---|---|
Stock Number: |
006005 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(APPswe)E1-2Dbo/J (Changed: 13-FEB-07
) |
| Genes & Alleles | APP; Prnp; Tg(Prnp-App/APPswe)E1-2Dbo; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator David Borchelt, McKnight Brain Inst, Univ of Florida Generation N14?N5 (20-DEC-07) Strain Description
These transgenic mice express a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter. Mice that are hemizygous for the transgene are viable and fertile. More than half of the female hemizygous mice do not survive past 15 months of age. This mutant mouse strain may be useful in studies of Alzheimer's Disease.Strain Development
A transgenic construct a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter was injected into fertilized C57BL/6J X C3HeJ F2 mouse eggs. Founder line E1-2 was established. The mice were then backcrossed to C57BL/6J for more than 14 generations.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(Prnp-App/APPswe)E1-2Dbo | ||
|---|---|---|---|
| Allele Name | transgene insertion E1-2, David R Borchelt | ||
| Common Name(s) | E1-2 line; Mo/Hu APPswe; Mo/Hu APPswe line E1-2; line E1-2; | ||
| Strain of Origin | (C57BL/6J x C3H/HeJ)F2 | ||
| Expressed Gene | APP, amyloid beta (A4) precursor protein, human | ||
| Promoter | Prnp, prion protein, mouse, laboratory | ||
| General Note | Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 and C3-3 (Tg(APP695)3Dbo). They were estimated, on the basis of Southern blot analysis, to carry 3-10 copies of the transgene. | ||
| Molecular Note | This transgene expresses a modified mouse amyloid precursor protein cDNA encoding the 695-amino acid isoform with a "humanized" Abeta domain amino acid sequence that includes the familial Alzheimer disease- (FAD-) associated Swedish double mutation (Lys595Asn and Met596Leu). The cDNA was inserted into an expression cassette containing the promoter, 5' intronic and 3' untranslated region sequences of the mouse prion protein gene. [MGI Ref ID J:80782] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Tg(APP)
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying other alleles of APP
006409 129S1.Cg-Tg(APPSw)40Btla/J 006555 A.129(B6)-Tg(APPSw)40Btla/J 005300 B6.129-Tg(APPSw)40Btla/J 005301 B6.129S2-Tg(APP)8.9Btla/J 006406 B6.129S4-Tg(APPSwLon)96Btla/J 005864 B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/J 004662 B6.Cg-Tg(PDGFB-APP)5Lms/J 004661 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/1J 006293 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J 006006 B6.Cg-Tg(Prnp-APP)A-2Dbo/J 007049 B6.Cg-Tg(tetO-APPSwInd)885Dbo/J 004462 B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J 006004 B6C3-Tg(tetO-APPSwInd)885Dbo/J 007027 C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/J 006472 D2.129(B6)-Tg(APPSw)40Btla/J View Strains carrying other alleles of APP (15 strains)
Strains carrying other alleles of Prnp
View Strains carrying other alleles of Prnp (18 strains)
Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Room Number AX11
APP relatedNeurobiology Research
Alzheimer's Disease (strains expressing mutant APP)
Alzheimer's Disease
Mouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
Neurodegeneration
Selected Reference(s)
Additional ReferencesSavonenko AV; Xu GM; Price DL; Borchelt DR; Markowska AL. 2003. Normal cognitive behavior in two distinct congenic lines of transgenic mice hyperexpressing mutant APP SWE. Neurobiol Dis 12(3):194-211. [PubMed: 12742740] [MGI Ref ID J:109847]
| Strain Name: | B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J |
| Stock Number: | 006005 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form