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Strain Name:

B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J

Stock Number:

006005

Availability:

Repository- Live

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Former Name      B6.Cg-Tg(APPswe)E1-2Dbo/J    (Changed: 13-FEB-07 )
Genes & Alleles   APP;   Prnp;   Tg(Prnp-App/APPswe)E1-2Dbo;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemInbred x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator David Borchelt,   McKnight Brain Inst, Univ of Florida
GenerationN14?N5 (20-DEC-07)

Strain Description
These transgenic mice express a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter. Mice that are hemizygous for the transgene are viable and fertile. More than half of the female hemizygous mice do not survive past 15 months of age. This mutant mouse strain may be useful in studies of Alzheimer's Disease.

Strain Development
A transgenic construct a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter was injected into fertilized C57BL/6J X C3HeJ F2 mouse eggs. Founder line E1-2 was established. The mice were then backcrossed to C57BL/6J for more than 14 generations.

Related Disease (OMIM) Terms

Alzheimer Disease; AD
Mammalian Phenotype Terms assigned by genotype

Tg(Prnp-App/APPswe)E1-2Dbo/0

        B6.C3-Tg(Prnp-App/APPswe)E1-2Dbo
  • nervous system phenotype
  • amyloid beta deposits (MGI Ref ID J:109847)
    • 14-15 month old mutants backcrossed 5- or 10 generations tend to have higher accumulation of amyloid compared to Tg(APP695)3Dbo at that age
  • behavior/neurological phenotype
  • abnormal locomotor activity (MGI Ref ID J:109847)
    • in radial maze testing, male mutants backcrossed 5-7 generations show high motor reactivity compared to females or control animals, and entered the closest arm of the maze which prevented them from learning the task
    • decreased vertical activity (MGI Ref ID J:109847)
      • at 12-13 months, mice backcrossed 5-7 generations show fewer rearing reactions compared to nontransgenic littermates
    • hypoactivity (MGI Ref ID J:109847)
      • at 12-13 months, males backcrossed 5-7 generations are less active than controls in inner cells of open field
  • abnormal object recognition memory (MGI Ref ID J:109847)
    • as females age (13-14 months of age), significant difference compared to female controls is observed in trials with longest (<60 minute) delays
  • abnormal spatial learning (MGI Ref ID J:109847)
    • 12-13 month old males and females backcrossed 5-7 generations show poorer performance in probe trials in the Morris water maze; females spent less time in correct quadrant until fourth consectutive session
    • upon repeat testing of the same females one month later, difference between mutants and controls is more robust; repeated testing had no effect on performance
  • increased anxiety-related response (MGI Ref ID J:109847)
    • at 12-13 months, females backcrossed 5-7 generations are less active in visiting open arms in plus maze; combined with reduced rearing reactions, females may have higher anxiety levels
  • other phenotype
  • amyloid beta deposits (MGI Ref ID J:109847)
    • 14-15 month old mutants backcrossed 5- or 10 generations tend to have higher accumulation of amyloid compared to Tg(APP695)3Dbo at that age

Gene & Allele Details

Allele Symbol Tg(Prnp-App/APPswe)E1-2Dbo
Allele Name transgene insertion E1-2, David R Borchelt
Common Name(s) E1-2 line; Mo/Hu APPswe; Mo/Hu APPswe line E1-2; line E1-2;
Strain of Origin(C57BL/6J x C3H/HeJ)F2
Expressed Gene APP, amyloid beta (A4) precursor protein, human
Promoter Prnp, prion protein, mouse, laboratory
General Note Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 and C3-3 (Tg(APP695)3Dbo). They were estimated, on the basis of Southern blot analysis, to carry 3-10 copies of the transgene.
Molecular Note This transgene expresses a modified mouse amyloid precursor protein cDNA encoding the 695-amino acid isoform with a "humanized" Abeta domain amino acid sequence that includes the familial Alzheimer disease- (FAD-) associated Swedish double mutation (Lys595Asn and Met596Leu). The cDNA was inserted into an expression cassette containing the promoter, 5' intronic and 3' untranslated region sequences of the mouse prion protein gene. [MGI Ref ID J:80782]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(APP)

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as hemizygotes.
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of APP     (15 strains)

View Strains carrying other alleles of Prnp     (18 strains)

Additional Web Information

Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease (strains expressing mutant APP)
Alzheimer's Disease

APP related

Mouse/Human Gene Homologs
Alzheimer's

Neurobiology Research
Alzheimer's Disease
Neurodegeneration

References

Selected Reference(s)

Savonenko AV; Xu GM; Price DL; Borchelt DR; Markowska AL. 2003. Normal cognitive behavior in two distinct congenic lines of transgenic mice hyperexpressing mutant APP SWE. Neurobiol Dis 12(3):194-211. [PubMed: 12742740]  [MGI Ref ID J:109847]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J
Stock Number: 006005

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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