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Former Name	B6.129-Ly9tm1Mckn/J    (Changed: 19-FEB-07 )
Genes & Alleles	Ly9;   Ly9tm1Mckn;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator David McKean,   Mayo Clinic Generation N2pN1

Strain Description
Mice homozygous for the targeted mutation are viable, fertile, and do not display any gross anatomical or behavioral abnormalities. FACS analysis of splenocytes confirms homozygous mice do not express the endogenous protein. When stimulated with anti -CD3, splenocytes cultured from homozygous mice show significant early T cell activation defects (proliferation and IL-2 production). When stimulated with anti-TCR and anti-CD28, CD4⁺ T splenocytes cultured from homozygous mice exhibit mild defects in Th2 cytokine production, but respond normally to cytokine stimulation. These mutant mice may be useful in studies of innate and adaptive immunity, lymphocyte signaling/costimulation, T cell activation, Th1/Th2 cytokine function, and CD2/SLAM family signal transduction.

Strain Development
A targeting vector was designed to replace the 3' portion of exon 2 of the endogenous gene with a neomycin resistance gene. The construct was injected into 129/SvEES embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and next implanted into pseudopregnant surrogate mothers. Chimeric mice were subsequently bred with C57BL/6 mice for one generation before arrival at The Jackson Laboratory. Upon arrival, mutant mice were backcrossed to C57BL/6J for approximately 2 generations.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Ly9tm1Mckn/Ly9tm1Mckn         involves: 129/Sv * C57BL/6 immune system phenotype abnormal T-helper 2 cell morphology (MGI Ref ID J:105482) following in vitro stimulation with low doses of anti-T cell receptor (anti-TCR) and anti-CD28 antibodies, the response of purified mutant splenic CD4+ T cells previously cultured under Th1/Th2 neutral conditions was skewed toward Th1, as indicated by their reduced expression, relative to wild type cells, of IL-4; mutant and wild type CD4+ T cells' responses were indistinguishable if stimulation followed preculture under Th1 or Th2 polarizing conditionss, and optimization of anti-TCR/anti-CD28 doses reduced the response difference to insignificance abnormal cytokine secretion (MGI Ref ID J:105482) homozygous mutant splenocytes stimulated in vitro with anti-CD3 antibody produced significantly less interleukin 2 (IL2) than did those from wild type mice decreased T cell proliferation (MGI Ref ID J:105482) homozygous mutant splenocytes stimulated in vitro with anti-CD3 antibody proliferated significantly less than did those from wild type mice hematopoietic system phenotype abnormal T-helper 2 cell morphology (MGI Ref ID J:105482) following in vitro stimulation with low doses of anti-T cell receptor (anti-TCR) and anti-CD28 antibodies, the response of purified mutant splenic CD4+ T cells previously cultured under Th1/Th2 neutral conditions was skewed toward Th1, as indicated by their reduced expression, relative to wild type cells, of IL-4; mutant and wild type CD4+ T cells' responses were indistinguishable if stimulation followed preculture under Th1 or Th2 polarizing conditionss, and optimization of anti-TCR/anti-CD28 doses reduced the response difference to insignificance decreased T cell proliferation (MGI Ref ID J:105482) homozygous mutant splenocytes stimulated in vitro with anti-CD3 antibody proliferated significantly less than did those from wild type mice

Gene & Allele Details

Allele Symbol		Ly9tm1Mckn
Allele Name		targeted mutation 1, David J McKean
Mutation Made By		David McKean,   Mayo Clinic
Strain of Origin		129/Sv
ES Cell Line Strain		129
Gene Symbol and Name		Ly9, lymphocyte antigen 9
Chromosome		1
Gene Common Name(s)		AI893573; CD229; Lgp100; Ly-9; SLAMF3; T100; expressed sequence AI893573; hly9; mLY9;
General Note		Despite a significant defect in early T cell activation and a slight but significant defect in the Th2 response of CD4+ T cells, homozygous mutant mice mount appropriate T and B cell responses to lymphocytic choriomeningitis virus, their NKT cell development is normal, and mutant macrophages exhibit no defects in cytokine production or bacterial killing.
Molecular Note		A 3' part of exon 2 and all of intron 2 have been replaced by a neomycin resistance gene. Western blot analysis of thymocytes and FACS analysis of splenocytes from homozygous mutant mice using rabbit antisera against the extracellular domain detected no LY9 (CD229) protein on these cells. [MGI Ref ID J:105482]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Ly9^tm1Mckn

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes.

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction
Transcriptional Regulation

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (T cell deficiency)

Internal/Organ Research
Lymphoid Tissue Defects (T cell deficiency)

Research Tools
Immunology and Inflammation Research (T cell deficiency)
Immunology and Inflammation Research (T cell specific surface marker)

References

Selected Reference(s)

Graham DB; Bell MP; McCausland MM; Huntoon CJ; van Deursen J; Faubion WA; Crotty S; KcKean DJ. 2006. Ly9 (CD229)-deficient mice exhibit T cell defects yet do not share several phenotypic characteristics associated with SLAM- and SAP-deficient mice J Immunol 176(1):291-300. [PubMed: 16365421]  [MGI Ref ID J:105482]

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Price and Supply Information

Strain Name:	B6;129-Ly9tm1Mckn/J
Stock Number:	006011

Price Details

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Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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