Strain Name: |
NOD.Cg-Rag1tm1Mom H2-Ab1tm1Gru Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell/EllJ |
|---|---|
Stock Number: |
006024 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD.Cg-Rag1tm1Mom H2-Ab1tm1Gru Tg(CD4,HLA-DQA1*0102,HLA-DQB1*0602)1Ell/EllJ (Changed: 07-NOV-06
) |
|
NOD.Cg-Rag1tm1Mom H2-Ab1tm1Gru Tg(CD4, HLA-DQA*0102, HLA-DQB*0602)1Ell/EllJ (Changed: 26-JAN-06
) | |
|
NOD.Cg-Rag1tm1Mom Ab1tm1Gru Tg(CD4, HLA-DQA*0102, HLA-DQB*0602)1Ell/EllJ (Changed: 25-JAN-06
) | |
| Genes & Alleles | CD4; H2-Ab1; H2-Ab1tm1Gru; HLA-DQA1; HLA-DQB1; Rag1; Rag1tm1Mom; Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain NOD/ShiLtJ Donating Investigator John Elliott, University of Alberta H2 Haplotype g7 Appearance
pink-eyed, albinoStrain Description
Rag1 and H2-Ab1 deficient NOD mice carrying transgenes encoding humanized CD4 and HLA-DQ6 lack T and B cells and are free of autoimmune diabetes and cardiomyopathy, and do not develop thyroiditis.This model is useful in adoptive transfer experiments and in studying MHC class II-based resistance and susceptibility in autoimmunity.
Strain Development
Congenic, H2-Ab1 deficient, NOD mice carrying transgenes encoding CD4 and a human genomic fragment containing the entire HLA-DQ6 gene (DQA1*0102, DQB1*0602, Stock No. 006023) were crossed to congenic Rag1 deficient NOD mice (Stock No. 003729), and were bred to homozygosity for the H2-Ab1tm1Gru and Rag1tm1Mom targeted mutations. The T1DR received this strain in 2006.
Gene & Allele Details
| Allele Symbol | H2-Ab1tm1Gru | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Michael J Grusby | ||
| Common Name(s) | Ab1tm1Gru; Abb-; Abbtm1; Abeta-; Abetab-; C2-; H2-Ab1-; H2-Ab1tm1Glm; I-Ab-; I-Abetab-; IAb-; IAbeta-; MHC class II-; [KO]Abb; mII-; | ||
| Mutation Made By | Michael Grusby, Harvard Medical School | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | H2-Ab1, histocompatibility 2, class II antigen A, beta 1 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | A beta; AI845868; Abeta; CELIAC1; H-2Ab; H2-Ab; HLA-DQB; I-Ab; I-Abeta; I-region-associated antigen 2; IAb; IDDM1; Ia-2; Ia2; Rmcs1; expressed sequence AI845868; histocompatibility 2, class II antigen A, beta; response to metastatic cancers 1; | ||
| Molecular Note | A neomycin selection cassette was inserted into exon 2. In addition, the ES cell line used was derived from the 129S2/SvPas strain, which carries a deletion in the promoter region of H2-Ea. Consequently, these MHC class II molecule-deficient mice lacked cell surface expression of both class II-A and class II-E MHC proteins. [MGI Ref ID J:74418] | ||
| Allele Symbol | Rag1tm1Mom | ||
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Common Name(s) | RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Rag1, recombination activating gene 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | MGC43321; RNF74; Rag-1; | ||
| Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [MGI Ref ID J:1934] [MGI Ref ID J:96036] | ||
| Allele Symbol | Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell | ||
| Allele Name | transgene insertion N8, John F Elliott | ||
| Common Name(s) | DQ6 transgene; HLA-DQ6 transgene; Tg(CD4,HLA-DQA1*0102,HLADQB1*0602)1Ell; | ||
| Strain of Origin | NOD/ShiLtJ | ||
| Expressed Gene | HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human | ||
| Expressed Gene | CD4, CD4 molecule, human | ||
| Expressed Gene | HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human | ||
| Promoter | HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human | ||
| Promoter | HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human | ||
| Molecular Note | The human CD4 gene lacking one exon but including its promoter was fused downstream of a mouse CD4 enhancer element then mixed with a large DNA fragment containing both HLA-DQA*0102 and HLA-DQB*0602 and injectedinto NOD/LtJ one celled embryos. A single founder line is maintained. [MGI Ref ID J:113267] | ||
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
H2-Ab1tm1Gru
Rag1tm1Mom
Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell
Colony Maintenance
| Breeding & Husbandry | These mice are immunodeficient and need to be maintained in a high barrier. Affected genotype: Rag1tm1Mom/ Rag1tm1Mom, Ab1tm1Gru/Ab1tm1Gru , Tg/? Breeder pairs: Rag1tm1Mom/ Rag1tm1Mom, Ab1tm1Gru/Ab1tm1Gru, Tg/? x same Genotypes available: Rag1tm1Mom/ Rag1tm1Mom, Ab1tm1Gru/Ab1tm1Gru, Tg/? |
|---|
Related Strains
Strains carrying H2-Ab1tm1Gru allele
View Strains carrying H2-Ab1tm1Gru (5 strains)
006023 NOD.Cg-H2-Ab1tm1Gru Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell/EllJ
006021 NOD.Cg-H2-Ab1tm1Gru Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell/EllJ 006022 NOD.Cg-Rag1tm1Mom H2-Ab1tm1Gru Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell/EllJ
002194 B6;CBA-Tg(CD3E)26Cpt/J 002506 STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
H2-Ab1tm1Gru relatedDiabetes and Obesity Research
Islet Transplantation Studies
Type 1 Diabetes (IDDM) Analysis Strains (NOD Congenics with Mutations Affecting Immunocompetence)
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Immunodeficiency (MHC class II deficient)
Research Tools
Immunology and Inflammation Research (MHC class II defects)
Rag1tm1Mom relatedImmunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)
Hematological Research
Immunological Defects (B and T cell deficiency)
Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)
Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)
References
Selected Reference(s)
Additional ReferencesHayward SL; Suzuki K; Elliott JF. 2007. A radioligand binding assay to measure anti-thyroperoxidase autoantibodies in mice. J Immunol Methods 323(2):114-22. [PubMed: 17482640] [MGI Ref ID J:124227]
Price and Supply Information
| Strain Name: | NOD.Cg-Rag1tm1Mom H2-Ab1tm1Gru Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell/EllJ |
| Stock Number: | 006024 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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