Strain Name:

B6;129S6-Epha2tm1Jrui/J

Stock Number:

006028

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
This Epha2 knock-out strain may be useful in studies of postnatal angiogenesis, as well as adenocarcinoma and breast cancer research.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   02-JUN-06
Specieslaboratory mouse
GenerationF?+F21 (26-NOV-14)
Generation Definitions
 
Donating Investigator Joseph C Ruiz,   Indiana University School of Medicine

Description
Mice homozygous for this targeted mutation are viable and fertile with no overt developmental or behavioral abnormalities. The Jackson Laboratory is distributing these mice on their original C57BL/6;129S6 mixed background. The published phenotype of these mutant mice is described below.

In mutant mice of a mixed BALB/c, C57BL/6, and 129S6 background, murine pulmonary microvascular endothelial cells (MPMEC) isolated from homozygotes express no endogenous protein. MPMEC show impaired ephrin-A1-induced vascular assembly and defective migration both in vitro and in vivo. In addition, MPMEC from homozygous mice exhibit decreased angiogenesis and fail to activate Rac1 in response to ephrin-A1 in vivo. Mutant mice crossed to BALB/c for 7 generations are protected from tumor progression, angiogenesis and metastasis following metastatic mammary adenocarcinoma cell transplantation. These mutant mice may be useful in studies of postnatal angiogenesis (endothelial cell migration, assembly into new tubules, and cytoskeletal regulation), as well as adenocarcinoma and breast cancer research.

Development
A targeting vector was designed to insert a bacterial neo casette into exon 5 (nucleotide 1372, corresponding to amino acid residue 426 in the extracellular domain) of the endogenous gene. This construct was microinjected into 129S6/SvEvTac-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females for one generation. Heterozygous offspring were interbred to produce homozygous mice. Mutant mice were maintained by breeding non-agouti siblings for more than 10 years before arrival at The Jackson Laboratory.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Epha2tm1Jrui allele
006070   CBy.129S6(B6)-Epha2tm1Jrui/J
View Strains carrying   Epha2tm1Jrui     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Cataract 6, Multiple Types; CTRCT6   (EPHA2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Epha2tm1Jrui/Epha2tm1Jrui

        involves: 129 * BALB/c * C57BL/6
  • cardiovascular system phenotype
  • abnormal angiogenesis
    • endothelial cells fail to assemble into capillary-like structure (vascular assembly) and to migrate in response to ephrin A1 (Efna1) in vitro   (MGI Ref ID J:89275)
    • mutants show impaired ephrin A1-induced angiogenesis in vivo   (MGI Ref ID J:89275)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Other
      tumor metastasis
Tumor Resistance
Tumor Suppressor Genes

Cardiovascular Research
Other
Vascular Defects

Research Tools
Cancer Research
      anti-tumor activity
      tumor immunology
Cardiovascular Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Epha2tm1Jrui
Allele Name targeted mutation 1, Joseph C Ruiz
Allele Type Targeted (Null/Knockout)
Common Name(s) Epha2-; Epha2tm1Jinc;
Mutation Made By Joseph Ruiz,   Indiana University School of Medicine
Strain of Origin129S/SvEv-Gpi1
ES Cell Line NameCCE/EK.CCE
ES Cell Line Strain129S/SvEv-Gpi1
Gene Symbol and Name Epha2, Eph receptor A2
Chromosome 4
Gene Common Name(s) ARCC2; AW545284; CTPA; CTPP1; CTRCT6; ECK; Eck; Myk2; Sek-2; Sek2; epithelial cell kinase; expressed sequence AW545284;
Molecular Note A neo cassette was inserted at nucleotide 1372, within the sequence encoding the extracellular domain. Although the resultant transcript putatively encodes a nonfunctional protein truncated at amino acid 426, no protein was detected by Western blot analysis of murine pulmonary microvascular endothelial cells from mutant mice. [MGI Ref ID J:89275]

Genotyping

Genotyping Information

Genotyping Protocols

Epha2tm1Jrui-Alterntae 3, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Brantley-Sieders DM; Caughron J; Hicks D; Pozzi A; Ruiz JC; Chen J. 2004. EphA2 receptor tyrosine kinase regulates endothelial cell migration and vascular assembly through phosphoinositide 3-kinase-mediated Rac1 GTPase activation. J Cell Sci 117(Pt 10):2037-49. [PubMed: 15054110]  [MGI Ref ID J:89275]

Additional References

Epha2tm1Jrui related

Amato KR; Wang S; Hastings AK; Youngblood VM; Santapuram PR; Chen H; Cates JM; Colvin DC; Ye F; Brantley-Sieders DM; Cook RS; Tan L; Gray NS; Chen J. 2014. Genetic and pharmacologic inhibition of EPHA2 promotes apoptosis in NSCLC. J Clin Invest 124(5):2037-49. [PubMed: 24713656]  [MGI Ref ID J:212762]

Brantley-Sieders DM; Fang WB; Hicks DJ; Zhuang G; Shyr Y; Chen J. 2005. Impaired tumor microenvironment in EphA2-deficient mice inhibits tumor angiogenesis and metastatic progression. FASEB J 19(13):1884-6. [PubMed: 16166198]  [MGI Ref ID J:102664]

Brantley-Sieders DM; Zhuang G; Hicks D; Fang WB; Hwang Y; Cates JM; Coffman K; Jackson D; Bruckheimer E; Muraoka-Cook RS; Chen J. 2008. The receptor tyrosine kinase EphA2 promotes mammary adenocarcinoma tumorigenesis and metastatic progression in mice by amplifying ErbB2 signaling. J Clin Invest 118(1):64-78. [PubMed: 18079969]  [MGI Ref ID J:130805]

Carpenter TC; Schroeder W; Stenmark KR; Schmidt EP. 2012. Eph-A2 promotes permeability and inflammatory responses to bleomycin-induced lung injury. Am J Respir Cell Mol Biol 46(1):40-7. [PubMed: 21799118]  [MGI Ref ID J:191913]

Cheng C; Ansari MM; Cooper JA; Gong X. 2013. EphA2 and Src regulate equatorial cell morphogenesis during lens development. Development 140(20):4237-45. [PubMed: 24026120]  [MGI Ref ID J:204508]

Hahn AS; Kaufmann JK; Wies E; Naschberger E; Panteleev-Ivlev J; Schmidt K; Holzer A; Schmidt M; Chen J; Konig S; Ensser A; Myoung J; Brockmeyer NH; Sturzl M; Fleckenstein B; Neipel F. 2012. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus. Nat Med 18(6):961-6. [PubMed: 22635007]  [MGI Ref ID J:186781]

Okazaki T; Ni A; Baluk P; Ayeni OA; Kearley J; Coyle AJ; Humbles A; McDonald DM. 2009. Capillary defects and exaggerated inflammatory response in the airways of EphA2-deficient mice. Am J Pathol 174(6):2388-99. [PubMed: 19443703]  [MGI Ref ID J:148922]

Rao A; Taylor JL; Chi-Sabins N; Kawabe M; Gooding WE; Storkus WJ. 2012. Combination therapy with HSP90 inhibitor 17-DMAG reconditions the tumor microenvironment to improve recruitment of therapeutic T cells. Cancer Res 72(13):3196-206. [PubMed: 22552283]  [MGI Ref ID J:189322]

Shi Y; De Maria A; Bennett T; Shiels A; Bassnett S. 2012. A role for epha2 in cell migration and refractive organization of the ocular lens. Invest Ophthalmol Vis Sci 53(2):551-9. [PubMed: 22167091]  [MGI Ref ID J:191524]

Vaught D; Chen J; Brantley-Sieders DM. 2009. Regulation of mammary gland branching morphogenesis by EphA2 receptor tyrosine kinase. Mol Biol Cell 20(10):2572-81. [PubMed: 19321667]  [MGI Ref ID J:175142]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice are bred.
Mating SystemHomozygote x Homozygote         (Female x Male)   02-JUN-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Epha2tm1Jrui  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Epha2tm1Jrui x Homozygous for Epha2tm1Jrui  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Epha2tm1Jrui  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Epha2tm1Jrui x Homozygous for Epha2tm1Jrui  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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