Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N5F2pN1 Generation Definitions   Donating Investigator David J Anderson,   California Institute of Technology, HHMI

Description
Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 1 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in studying the cellular and molecular mechanisms underlying vasculogenesis and angiogenesis, the topography of neovascularization, and adult neovascularization, including tumor angiogenesis.

To test the effectiveness of this model, these mutant mice were bred to an endothelial-specific Cre-expressing transgenic mice, Tg(Tek-cre)12Flv (Stock No. 004128) . Offspring homozygous for the Cre-mediated exon 1 deletion show angiogenic remodeling defects and embryonic death identical to homozygous Efnb2^tm1And mice (see Stock No. 006039).

Development
A targeting vector was designed to insert a loxP site upstream of the transcriptional start site and a loxP-flanked PGKneo cassette downstream of exon 1 of the endogenous gene. This construct was microinjected into 129S7/SvEvBrd-derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre-expressing vector to remove the PGKneo cassette. Cells harboring the recombined loxP-flanked exon 1 were injected into blastocysts. Chimeric males were bred to C57BL/6 females. Heterozygous mice were bred to C57BL/6 for 3 generations before being made homozygous. After arriving at The Jackson Laboratory, mutant mice were bred with C57BL/6J for more than two generations.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Efnb2

006039	B6.129S7-Efnb2tm1And/J
007843	B6;129S4-Efnb2tm2Sor/J

View Strains carrying other alleles of Efnb2     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Efnb2^tm1And/Efnb2^tm2And Tg(Tek-cre)12Flv/0

        involves: 129S7/SvEvBrd * C57BL/6   (conditional)

• mortality/aging
• complete embryonic lethality during organogenesis
  • mutant embryos die by E11   (MGI Ref ID J:74909)
• cardiovascular system phenotype
• abnormal heart looping
  • heart looping defects   (MGI Ref ID J:74909)
• abnormal vascular development
  • vasculature is disorganized and less developed   (MGI Ref ID J:74909)
• • abnormal angiogenesis
    • E9.5 mutants exhibit a failure of anterior cardinal vein assembly   (MGI Ref ID J:74909)
    • the capillary bed of the head appears dilated and arrested at the primary plexus stage   (MGI Ref ID J:74909)
    • embryos exhibit an arrest in intersomitic vessel angiogenesis at the primary plexus stage; the vessels appear fused dorsally with little or no branching   (MGI Ref ID J:74909)
  • • abnormal developmental vascular remodeling
      • E9.5 mutants exhibit a failure of anterior cardinal vein assembly and remodeling   (MGI Ref ID J:74909)
  • abnormal vitelline vasculature morphology
    • yolk sac is a homogeneous capillary bed at E9.5, indicating arrest of artery and vein development at the primary plexus stage   (MGI Ref ID J:74909)
• absent myocardial trabeculae
  • little or no myocardial trabecular extensions by E9   (MGI Ref ID J:74909)
• enlarged heart
  • hearts are swollen at E9.5   (MGI Ref ID J:74909)
• embryogenesis phenotype
• abnormal vitelline vasculature morphology
  • yolk sac is a homogeneous capillary bed at E9.5, indicating arrest of artery and vein development at the primary plexus stage   (MGI Ref ID J:74909)
• decreased embryo size
  • evident at E10   (MGI Ref ID J:74909)
• embryonic growth arrest
  • embryos are developmentally less advanced at E9.5   (MGI Ref ID J:74909)
• growth/size phenotype
• decreased embryo size
  • evident at E10   (MGI Ref ID J:74909)
• muscle phenotype
• absent myocardial trabeculae
  • little or no myocardial trabecular extensions by E9   (MGI Ref ID J:74909)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Developmental Biology Research
Internal/Organ Defects
      heart
      heart: vasculature

Research Tools
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Internal/Organ Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Efnb2tm2And
Allele Name		targeted mutation 2, David J Anderson
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Efnb2flox; EphrinB2LoxP;
Mutation Made By		David Anderson,   California Institute of Technology, HHMI
Strain of Origin		129S7/SvEvBrd-Hprt<+>
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name		Efnb2, ephrin B2
Chromosome		8
Gene Common Name(s)		ELF-2; EPLG5; Epl5; HTKL; Htk-L; LERK-5; LERK5; NLERK-1; eph-related receptor tyrosine kinase ligand 5;
Molecular Note		A loxP site was inserted 45 bp upstream in the 5' UTR and a floxed PGKneo cassette was inserted downstream of the first exon. The floxed PGKneo cassette was removed by transient Cre expression in the ES cells which resulted in a floxed intact exon I. [MGI Ref ID J:74909]

Genotyping

Genotyping Information

Genotyping Protocols

Efnb2^tm2And, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Gerety SS; Anderson DJ. 2002. Cardiovascular ephrinB2 function is essential for embryonic angiogenesis. Development 129(6):1397-410. [PubMed: 11880349]  [MGI Ref ID J:74909]

Additional References

Efnb2^tm2And related

Cejalvo T; Munoz JJ; Tobajas E; Fanlo L; Alfaro D; Garcia-Ceca J; Zapata A. 2013. Ephrin-B-dependent thymic epithelial cell-thymocyte interactions are necessary for correct T cell differentiation and thymus histology organization: relevance for thymic cortex development. J Immunol 190(6):2670-81. [PubMed: 23408838]  [MGI Ref ID J:193677]

Chenaux G; Henkemeyer M. 2011. Forward signaling by EphB1/EphB2 interacting with ephrin-B ligands at the optic chiasm is required to form the ipsilateral projection. Eur J Neurosci 34(10):1620-33. [PubMed: 22103419]  [MGI Ref ID J:184158]

Coate TM; Raft S; Zhao X; Ryan AK; Crenshaw EB 3rd; Kelley MW. 2012. Otic Mesenchyme Cells Regulate Spiral Ganglion Axon Fasciculation through a Pou3f4/EphA4 Signaling Pathway. Neuron 73(1):49-63. [PubMed: 22243746]  [MGI Ref ID J:179898]

Egawa G; Osawa M; Uemura A; Miyachi Y; Nishikawa S. 2009. Transient expression of ephrin b2 in perinatal skin is required for maintenance of keratinocyte homeostasis. J Invest Dermatol 129(10):2386-95. [PubMed: 19571816]  [MGI Ref ID J:157054]

Kim YH; Hu H; Guevara-Gallardo S; Lam MT; Fong SY; Wang RA. 2008. Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis. Development 135(22):3755-64. [PubMed: 18952909]  [MGI Ref ID J:144857]

Red-Horse K; Ueno H; Weissman IL; Krasnow MA. 2010. Coronary arteries form by developmental reprogramming of venous cells. Nature 464(7288):549-53. [PubMed: 20336138]  [MGI Ref ID J:158886]

Zhao C; Irie N; Takada Y; Shimoda K; Miyamoto T; Nishiwaki T; Suda T; Matsuo K. 2006. Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab 4(2):111-21. [PubMed: 16890539]  [MGI Ref ID J:129733]

Zhao J; Yuan G; Cendan CM; Nassar MA; Lagerstrom MC; Kullander K; Gavazzi I; Wood JN. 2010. Nociceptor-expressed ephrin-B2 regulates inflammatory and neuropathic pain. Mol Pain 6:77. [PubMed: 21059214]  [MGI Ref ID J:168624]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes or homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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