Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N5F2pN1   Donating Investigator David Anderson,   California Institute of Technology

Description
Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 1 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in studying the cellular and molecular mechanisms underlying vasculogenesis and angiogenesis, the topography of neovascularization, and adult neovascularization, including tumor angiogenesis.

To test the effectiveness of this model, these mutant mice were bred to an endothelial-specific Cre-expressing transgenic mice, Tg(Tek-cre)12Flv (Stock No. 004128) . Offspring homozygous for the Cre-mediated exon 1 deletion show angiogenic remodeling defects and embryonic death identical to homozygous Efnb2^tm1And mice (see Stock No. 006039).

Development
A targeting vector was designed to insert a loxP site upstream of the transcriptional start site and a loxP-flanked PGKneo cassette downstream of exon 1 of the endogenous gene. This construct was microinjected into 129S7/SvEvBrd-derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre-expressing vector to remove the PGKneo cassette. Cells harboring the recombined loxP-flanked exon 1 were injected into blastocysts. Chimeric males were bred to C57BL/6 females. Heterozygous mice were bred to C57BL/6 for 3 generations before being made homozygous. After arriving at The Jackson Laboratory, mutant mice were bred with C57BL/6J for more than two generations.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Efnb2

006039	B6.129S7-Efnb2tm1And/J
007843	B6;129S4-Efnb2tm2Sor/J

View Strains carrying other alleles of Efnb2     (2 strains)

Additional Web Information

Congenic Nomenclature
Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Efnb2^tm1And/Efnb2^tm2And Tg(Tek-cre)12Flv/0

        involves: 129S7/SvEvBrd * C57BL/6   (conditional)

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:74909)
  • mutant embryos died by E11
• cardiovascular system phenotype
• abnormal angiogenesis (MGI Ref ID J:74909)
  • the capillary bed of the head appears dilated and apparently arrested at the primary plexus stage
  • capillary ingrowth into the neural tube fails to occur apparent at E9.5
• enlarged heart (MGI Ref ID J:74909)
• failure of myocardial trabecular formation (MGI Ref ID J:74909)
  • the absence of myocardial trabecular extensions were apparent by E9.0
• embryogenesis phenotype
• abnormal vitelline vasculature (MGI Ref ID J:74909)
  • defects in yolk sac angiogenesis were apparent by E9.0
• reduced embryo size (MGI Ref ID J:74909)
  • evident at E10
• growth/size phenotype
• reduced embryo size (MGI Ref ID J:74909)
  • evident at E10
• muscle phenotype
• failure of myocardial trabecular formation (MGI Ref ID J:74909)
  • the absence of myocardial trabecular extensions were apparent by E9.0

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Developmental Biology Research
Internal/Organ Defects (heart)
Internal/Organ Defects (heart: vasculature)

Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Internal/Organ Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Efnb2tm2And
Allele Name		targeted mutation 2, David J Anderson
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		EphrinB2LoxP;
Mutation Made By		David Anderson,   California Institute of Technology
Strain of Origin		129S7/SvEvBrd-Hprt1<+>
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name		Efnb2, ephrin B2
Chromosome		8
Gene Common Name(s)		ELF-2; EPLG5; Epl5; HTKL; Htk-L; LERK-5; LERK5; MGC126226; MGC126227; MGC126228; NLERK-1; eph-related receptor tyrosine kinase ligand 5;
Molecular Note		A loxP site was inserted 45 bp upstream in the 5' UTR and a floxed PGKneo cassette was inserted downstream of the first exon. The floxed PGKneo cassette was removed by transient Cre expression in the ES cells which resulted in a floxed intact exon I. [MGI Ref ID J:74909]

Genotyping

Genotyping Information

Genotyping Protocols

Efnb2^tm2And, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Gerety SS; Anderson DJ. 2002. Cardiovascular ephrinB2 function is essential for embryonic angiogenesis. Development 129(6):1397-410. [PubMed: 11880349]  [MGI Ref ID J:74909]

Additional References

Efnb2^tm2And related

Zhao C; Irie N; Takada Y; Shimoda K; Miyamoto T; Nishiwaki T; Suda T; Matsuo K. 2006. Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab 4(2):111-21. [PubMed: 16890539]  [MGI Ref ID J:129733]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes or homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.