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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Generation N?+1F3 (01-NOV-07) Donating Investigator W. Scott Young III, National Institute of Mental Health Description
Hemizygous mice are viable and fertile with no gross or behavioral abnormalities. This transgene expresses an enhanced green fluorescent protein (EGFP) fused to the end of the neurophysin at the C-terminus of the oxytocin pre-prohormone. The transgene is selectively expressed in oxytocin-magnocellular neurons of the paraventricular and supraoptic nuclei of the hypothalamus. The fusion protein is faithfully trafficked to secretory granules and transported to neurosecretory terminals in the neurohypophysis, where the EGFP fluorescence undergoes depolarization-induced calcium-dependent secretion. Immunohistochemical detection of EGFP in individual oxytocin-magnocellular neurons is suggested as intrinsic fluorescence is low. However, the endogenous fluorescence in the neural lobes is sufficiently intense to image secretory events in individual oxytocin nerve terminals (neurosecretosomes) isolated from the posterior pituitary. These mice may be useful in studies of hormone biology, pharmacology, and to visualize neurosecretion.Development
A transgene containing the mouse oxytocin (Oxt) gene (including 0.47kb upstream of the transcriptional start site) and exons 2 and 3 of the vasopressin gene in the natural orientation was modified by inserting the enhanced green fluorescent protein (EGFP, Clontech) gene in-frame into the third exon of Oxt. This entire construct was injected into B6SJL fertilized eggs. Transgenic offspring (line AI-03) were bred together (and also periodically backcrossed to C57BL/6 mice) for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
002713 B6;129S-Oxttm1Wsy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Oxt/EGFP)AI03Wsy/0
involves: C57BL/6 * SJL
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:111959)
- no phenotypic analysis reported
This mouse can be used to support research in many areas including:
GFP relatedEndocrine Deficiency Research
Hypothalamus/Pituitary Defects
Neurobiology Research
Fluorescent protein expression in neural tissue
Neurotransmitter Receptor and Synaptic Vesicle Defects
Research Tools
Endocrine Deficiency Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Oxt/EGFP)AI03Wsy | ||
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| Allele Name | transgene insertion AI03, W Scott Young | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | AI03; | ||
| Mutation Made By | W. Scott Young III, National Institute of Mental Health | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | This transgene expresses an enhanced green fluorescent protein (EGFP) fused to the end of the neurophysin at the C-terminus of the oxytocin pre-prohormone. The transgene is selectively expressed in oxytocin-magnocellular neurons of the paraventricular and supraoptic nuclei of the hypothalamus. | ||
| Expressed Gene | Oxt, oxytocin, mouse, laboratory | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Oxt, oxytocin, mouse, laboratory | ||
| Molecular Note | The transgene containing the mouse oxytocin gene including 0.47 kb upstream of the transcriptional start site, and exons 2 and 3 of the vasopressin gene was modified by inserting the EGFP gene (Clonetech) in-frame into exon 3 of the oxytocin gene. The transgene is selectively expressed in oxytocin-magnocellular neurons of the paraventricular and supraoptic nuclei of the thalamus. [MGI Ref ID J:111958] [MGI Ref ID J:111959] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), MCA, vers. 2
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Tg(Oxt/EGFP)AI03Wsy relatedYoung WS rd; Iacangelo A; Luo XZ; King C; Duncan K; Ginns EI. 1999. Transgenic expression of green fluorescent protein in mouse oxytocin neurones. J Neuroendocrinol 11(12):935-9. [PubMed: 10583728] [MGI Ref ID J:111959]
Zhang BJ; Kusano K; Zerfas P; Iacangelo A; Young WS rd; Gainer H. 2002. Targeting of green fluorescent protein to secretory granules in oxytocin magnocellular neurons and its secretion from neurohypophysial nerve terminals in transgenic mice. Endocrinology 143(3):1036-46. [PubMed: 11861530] [MGI Ref ID J:111958]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice are bred to wildtype siblings. Mating System +/+ sibling x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Oxt/EGFP)AI03Wsy *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Oxt/EGFP)AI03Wsy x Noncarrier
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Oxt/EGFP)AI03Wsy *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Oxt/EGFP)AI03Wsy x Noncarrier
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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