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| These Actb-DsRed.T3 transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer and may be useful for widespread fluorescent protein expression. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(ACTB-DsRed*MST)1Nagy/J (Changed: 08-MAY-08 ) B6.Cg-Tg(ACTB-DsRed.MST)1Nagy/J (Changed: 20-FEB-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N5F6 (10-NOV-08) Donating Investigator Andras Nagy, Mount Sinai Hospital Description
Mice homozygous for this Actb-DsRed.T3 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description as published results become available.
Development
This strain is a derivative of Tg(ACTB-bgeo/DsRed.MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. ES cells containing the (ACTB-DsRed.MST) construct were aggregated with ICR outbred tetraploid embryos to generate chimeric mice. The resulting chimeric male animal was bred with an ICR outbred female. Progeny from this cross that were hemizygous for the transgene were bred to ICR outbred mice. The mice were then backcrossed to C57BL/6J for 5 generations. Of note, this "DsRed.MST" protein is erroneously designated "DsRed.T3" in the original publication.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
005441 STOCK Tg(CAG-DsRed*MST)1Nagy/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(CAG-DsRed*MST)1Nagy/0
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * ICR
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:109837)
- transgenic mice are viable and fertile
This mouse can be used to support research in many areas including:
DsRed relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-DsRed*MST)1Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andras Nagy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Actb-DsRed.T3; DsRed.T3; Tg(ACTB-DsRed*MST)1Nagy; Tg(ACTB-DsRed.MST)1Nagy; | ||
| Mutation Made By | Kristina Vintersten, Mount Sinai Hospital, SLRI | ||
| Strain of Origin | (129S6/SvEvTac x C57BL/6)F1 | ||
| ES Cell Line Name | G4 | ||
| ES Cell Line Strain | (129S6/SvEvTac x C57BL/6)F1 | ||
| Site of Expression | Widespread expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | This strain is a derivative of Tg(ACTB-Bgeo,-DsRed*MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. [MGI Ref ID J:109837] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic DsRed, MCA, vers. 4
Generic DsRed, QPCR, vers. 3
Generic DsRed, SEP PCR, vers. 2
Generic DsRed, STD PCR, vers. 1
Tg(DsRed), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40(4):241-6. [PubMed: 15593332] [MGI Ref ID J:109837]
Additional References
Tg(CAG-DsRed*MST)1Nagy relatedStarremans PG; Li X; Finnerty PE; Guo L; Takakura A; Neilson EG; Zhou J. 2008. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73(12):1394-405. [PubMed: 18385665] [MGI Ref ID J:140012]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes or hemizygotes. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $203.80 Female or Male Homozygous for Tg(CAG-DsRed*MST)1Nagy *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $407.60 Homozygous for Tg(CAG-DsRed*MST)1Nagy x Homozygous for Tg(CAG-DsRed*MST)1Nagy
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $265.00 Female or Male Homozygous for Tg(CAG-DsRed*MST)1Nagy *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $529.90 Homozygous for Tg(CAG-DsRed*MST)1Nagy x Homozygous for Tg(CAG-DsRed*MST)1Nagy
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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