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Common Names: DsRed.T3 ;    
These Actb-DsRed.T3 transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer and may be useful for widespread fluorescent protein expression.


Strain Information

Former Names B6.Cg-Tg(ACTB-DsRed*MST)1Nagy/J    (Changed: 08-MAY-08 )
B6.Cg-Tg(ACTB-DsRed.MST)1Nagy/J    (Changed: 20-FEB-07 )
Type Congenic; Transgenic;
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Mating SystemHomozygote x Homozygote         (Female x Male)   24-MAR-07
Specieslaboratory mouse
GenerationN5F13 (10-NOV-08)
Generation Definitions
Donating InvestigatorDr. Andras Nagy,   Mount Sinai Hospital

Mice homozygous for this Actb-DsRed.T3 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description as published results become available.

This strain is a derivative of Tg(ACTB-bgeo/DsRed.MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. ES cells containing the (ACTB-DsRed.MST) construct were aggregated with ICR outbred tetraploid embryos to generate chimeric mice. The resulting chimeric male animal was bred with an ICR outbred female. Progeny from this cross that were hemizygous for the transgene were bred to ICR outbred mice. The mice were then backcrossed to C57BL/6J for 5 generations. Of note, this "DsRed.MST" protein is erroneously designated "DsRed.T3" in the original publication.

Control Information

   000664 C57BL/6J
  Considerations for Choosing Controls

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012352   C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J
012361   C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J
010724   C57BL/6-Trim21tm1Hm/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
012943   C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
016617   C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J
012890   C57BL/6-Tg(Scgb1a1-Il17f,GFP)1Cdon/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
009655   C57BL/6J-Tg(Dcx-DsRed)14Qlu/J
007857   C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J
007860   C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
009593   C57BL/6J-Tg(Pomc-EGFP)1Low/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
024101   C57BL/6N-Il1r1tm1Quan/J
025400   C57BL/6N-Tg(Scn10a-EGFP)ALmp/J
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
007075   CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
026862   D2.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/SjJ
026854   D2.Cg-Tg(Thy1-Brainbow1.0)LLich/SjJ
026856   D2.FVB-Tg(GFAPGFP)14Mes/SjJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
003718   FVB-Tg(GadGFP)45704Swn/J
010947   FVB-Tg(Gstm5-EGFP)1Ilis/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005688   FVB-Tg(Rag2-EGFP)1Mnz/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
012429   FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
016573   FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd Tg(S100B-EGFP)1Wjt Tg(SMN2*delta7)4299Ahmb/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
008200   FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
009354   FVB/N-Tg(Dazl-EGFP)10Rarp/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
007800   FVB/N-Tg(Ins1-luc)VUPwrs/J
023410   FVB/N-Tg(tetO-Nog,-EGFP)53Hda/J
012370   FVB/NJ-Tg(Hspa1a-luc,-EGFP)2Chco/J
009618   NOD.129(B6)-Il12btm1Lky/JbsJ
013116   NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
013233   NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
009422   NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008547   NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
012881   STOCK Ascl1tm1Reed/J
008666   STOCK Fmn1tm1Made/J
013731   STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
017922   STOCK Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/J
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
024107   STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm93.1(tetO-GCaMP6f)Hze/HzeJ
017912   STOCK Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo/J
017921   STOCK Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo/J
017909   STOCK Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo/J
008876   STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
009349   STOCK Hprttm31(Ple67-EGFP)Ems/Mmjax
009594   STOCK Hprttm32(Ple112-EGFP)Ems/Mmjax
013749   STOCK Igs2tm1(ACTB-EGFP,-tdTomato)Luo/J
022976   STOCK Igs2tm1(ACTB-EGFP,-tdTomato)Zng/J
013751   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Luo/J
022977   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Zng/J
024108   STOCK Igs7tm93.1(tetO-GCaMP6f)Hze Tg(Camk2a-tTA)1Mmay/J
017932   STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
021458   STOCK Iis5tm1(ACTB-tdTomato,-EGFP)Luo/J
021457   STOCK Iis5tm2.1(ACTB-EGFP,-tdTomato)Luo/J
021461   STOCK Iis6tm1.1(ACTB-tdTomato,-EFGP)Luo/J
021460   STOCK Iis6tm2.1(ACTB-EFGP,-tdTomato)Luo/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
005692   STOCK Nphs1tm1Rkl/J
006741   STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)BMom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
009061   STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
007879   STOCK Stx1atm2Sud/J
014581   STOCK Trpm8tm1Apat/J
010911   STOCK Wt1tm1(EGFP/cre)Wtp/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
027617   STOCK Tg(CAG-EGFP*)1Rkol/RjulJ
011106   STOCK Tg(CAG-GFP*)1Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
025543   STOCK Tg(CAG-RFP*,-Dscam,-EGFP)1Pfu/J
011107   STOCK Tg(CAG-Venus)1Hadj/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
005105   STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
018322   STOCK Tg(Cp-EGFP)25Gaia/ReyaJ
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
016252   STOCK Tg(Hoxb7-Venus*)17Cos/J
006784   STOCK Tg(Ins1-Cerulean)24Hara/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
016921   STOCK Tg(Myh2-DsRed2)1Jrs/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
016922   STOCK Tg(Myh7-CFP)1Jrs/J
008579   STOCK Tg(PSCA-EGFP)1Witt/J
012452   STOCK Tg(Rr5-GFP/cre)1Sapc/J
006570   STOCK Tg(SMN2)89Ahmb Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
003658   STOCK Tg(TIE2GFP)287Sato/J
013162   STOCK Tg(Thy1-Clomeleon)12Gjau/J
013163   STOCK Tg(Thy1-Clomeleon)13Gjau/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
011108   STOCK Tg(Ttr-RFP)1Hadj/J
016981   STOCK Tg(Uchl1-HIST2H2BE/mCherry/EGFP*)FSout/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
003274   STOCK Tg(tetNZL)2Bjd/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
017918   STOCK Tg(tetO-MAML1*/EGFP)2Akar/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
View Fluorescent Protein Strains     (400 strains)

Strains carrying   Tg(CAG-DsRed*MST)1Nagy allele
017620   NOD.Cg-Prkdcscid Tg(CAG-DsRed*MST)1Nagy/KupwJ
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
View Strains carrying   Tg(CAG-DsRed*MST)1Nagy     (2 strains)

Strains carrying other alleles of ACTB
005483   129-Tg(CAG-EYFP)7AC5Nagy/J
004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
018979   B6.129(Cg)-Tg(CAG-EGFP)D4Nagy/KnwJ
004218   B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
021930   B6.129S1-Tg(CAG-EGFP)S1C2Tpo/KnwPeaJ
021146   B6.Cg-Fcgrttm1Dcr Prkdcscid Tg(CAG-FCGRT)276Dcr/DcrJ
004919   B6.Cg-Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ
025855   B6.Cg-Ptprca Lag3tm1Doi Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J
016919   B6.Cg-Rag1tm1Mom Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ
008705   B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
007241   B6.Cg-Tg(CAG-Serpine1)1Dgi/J
008111   B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J
008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
004682   B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J
005884   B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
025854   B6.FVB-Ptprca Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J
012436   B6;129S7-Tg(CAG-lacZ,-BMPR1A*,-EGFP)1Mis/Mmjax
008605   B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
008080   B6;C3-Tg(CAG-SAC/EGFP)35Rang/J
006465   B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
010967   B6;D2-Tg(Actb-Uprt)274Cdoe/J
009668   B6;D2-Tg(Actb-Uprt)372Cdoe/J
019102   B6N.Cg-Tg(CAG-cre/Esr1*)5Amc/CjDswJ
010545   C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
006662   C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J
006481   C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
017595   FVB.Cg-Tg(CAG-cre/Esr1*)5Amc/J
017881   FVB/NJ-Tg(CAG-Diap3)924Lesp/J
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
017932   STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
008813   STOCK Trpa1tm2Kykw Tg(CAG-cre/Esr1*)5Amc/J
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
006882   STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
006613   STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J
003919   STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
011106   STOCK Tg(CAG-GFP*)1Hadj/J
009678   STOCK Tg(CAG-RAB9A)500Repa/J
011107   STOCK Tg(CAG-Venus)1Hadj/J
008783   STOCK Tg(CAG-cre/Esr1*)5Amc Smn1tm3(SMN2/Smn1)Mrph Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb/J
004453   STOCK Tg(CAG-cre/Esr1*)5Amc/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
View Strains carrying other alleles of ACTB     (55 strains)

Strains carrying other alleles of RFP
006067   129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006041   129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
012687   B6(129S4)-Tg(SYN1-icre/mRFP1)9934Rdav/J
017456   B6(C)-Tg(CAG-mCherry,-GAL4)769Gsn/J
017457   B6(C)-Tg(CAG-mCherry,-GAL4)774Gsn/J
017614   B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J
021011   B6(D2)-Tg(CAG-Brainbow1.0)2Eggn/J
021012   B6(D2)-Tg(CAG-Brainbow1.0)3Eggn/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006080   B6.129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
006075   B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
022626   B6.129S6(SJL)-Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Mmjax
007914   B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007909   B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
017863   B6.Cg-Tg(Adora2a-Chrm3*,-mCherry)AD6Blr/J
008705   B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J
005884   B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J
025525   B6.Cg-Tg(Cx3cl1/mCherry)1Jung/J
016204   B6.Cg-Tg(Drd1a-tdTomato)6Calak/J
018410   B6.Cg-Tg(Pnkd*A7V*A9V,-DsRed)671Ljp/J
022146   B6.Cg-Tg(Pnkd*A7V*A9V,-DsRed)704Ljp/J
022147   B6.Cg-Tg(Pnkd,-DsRed)445Ljp/J
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
013082   B6.FVB-Tg(Per2-DsRed*T3)12Obr/Mmjax
012476   B6;129-Pax7tm2.1(cre/ERT2)Fan/J
010984   B6;129P-Upk1btm1Pzg/J
013139   B6;129P2-Ifitm3tm1(RFP)Pzg/J
006720   B6;129P2-Olfr124tm4Mom/MomJ
006713   B6;129P2-Olfr545tm2Mom/MomJ
010983   B6;129S-Id3tm1Pzg/J
010986   B6;129S-Osr2tm1Pzg/J
018128   B6;129S-Tg(Prox1-tdTomato)12Nrud/J
007908   B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007905   B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
017954   B6;C-Tg(CAG-Epha4/Efna5,-mCherry)1Slp/J
018936   B6;C-Tg(Erv4-tdTomato)449Slp/J
008605   B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
007984   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007982   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)8Mom/MomJ
007983   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)9Mom/MomJ
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
017496   B6;D-Tg(Lfng-TagRFP/cre/ERT2)8Amc/J
018682   B6;D-Tg(Meox1-TagRFP/cre/ERT2)30Amc/J
018683   B6;D-Tg(Wnt11-TagRFP/cre/ERT2)28Amc/J
013137   B6;D2-Tg(Akr1b7-RFP)9Amc/J
021577   B6;D2-Tg(Myh6*-mCherry)2Mik/J
015853   B6;DBA-Tg(Cited1-TagRFP)26Amc/J
008374   C57BL/6-Foxp3tm1Flv/J
027139   C57BL/6-Tg(CAG-RFP/GFP/Map1lc3b)1Hill/J
011086   C57BL/6-Tg(Cck-cre)CKres/J
024046   C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J
022766   C57BL/6-Tg(Prox1-tdTomato)12Nrud/J
027395   C57BL/6-Tg(Pvalb-tdTomato)15Gfng/J
009655   C57BL/6J-Tg(Dcx-DsRed)14Qlu/J
021119   C57BL/6J-Tg(Dlx2-cre,-mCherry)4Grsr/GrsrJ
021423   C57BL/6J-Tg(Dlx2-cre,-mCherry)9Grsr/GrsrJ
018754   C57BL/6J-Tg(Tbx22,-cre,-mCherry)1Grsr/GrsrJ
026862   D2.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/SjJ
026854   D2.Cg-Tg(Thy1-Brainbow1.0)LLich/SjJ
018542   FVB-Tg(Kdr-mCherry)1Medi/J
021853   FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J
018067   FVB-Tg(Prism)1849Htz/J
018071   FVB-Tg(Prism)1861Htz/J
018068   FVB-Tg(Prism)1989Htz/J
026690   FVB/N-Tg(Ucp1-luc2,-tdTomato)1Kajim/J
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
013749   STOCK Igs2tm1(ACTB-EGFP,-tdTomato)Luo/J
017530   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Luo Trp53tm1Tyj Nf1tm1Par/J
013751   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
017472   STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
014177   STOCK Tg(Afp-mCherry)1Hadj/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
017919   STOCK Tg(CAG-EGFP,-dsRed2/RNAi:Tardbp)6Zxu/J
013753   STOCK Tg(CAG-KikGR)33Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
025543   STOCK Tg(CAG-RFP*,-Dscam,-EGFP)1Pfu/J
017934   STOCK Tg(CAG-dsRed2/RNAi:Tardbp)6Zxu/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
017465   STOCK Tg(Col10a1-mCherry)3Pmay/J
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
016921   STOCK Tg(Myh2-DsRed2)1Jrs/J
024850   STOCK Tg(Sp7/mCherry)2Pmay/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
017470   STOCK Tg(Tnc-mCherry)2Pmay/J
011108   STOCK Tg(Ttr-RFP)1Hadj/J
016981   STOCK Tg(Uchl1-HIST2H2BE/mCherry/EGFP*)FSout/J
017906   STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
View Strains carrying other alleles of RFP     (96 strains)

Additional Web Information

Fluorescent Proteins/lacZ Systems


Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.


        involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCr * ICR
  • normal phenotype
  • no abnormal phenotype detected
    • transgenic mice are viable and fertile   (MGI Ref ID J:109837)
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Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Developmental Biology Research
Fluorescent Proteins

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Tg(CAG-DsRed*MST)1Nagy
Allele Name transgene insertion 1, Andras Nagy
Allele Type Transgenic (Reporter)
Common Name(s) Actb-DsRed.T3; CAG-DsRed; DsRed.T3; Tg(ACTB-DsRed*MST)1Nagy; Tg(ACTB-DsRed.MST)1Nagy;
Mutation Made By Kristina Vintersten,   Mount Sinai Hospital, SLRI
Strain of Origin(129S6/SvEvTac x C57BL/6NCrl)F1
ES Cell Line NameG4
ES Cell Line Strain(129S6/SvEvTac x C57BL/6NCrl)F1
Site of ExpressionWidespread expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle.
Expressed Gene RFP, Red Fluorescent Protein, coral
Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc).
Promoter ACTB, actin, beta, chicken
General Note Erratum publ Genesis 42:218 (2005) correction to original (2004) publication: The red fluorescent protein used is DsRed.MST, not DsRed.T3 as J:102837. DsRed.MST differs from DsRed.T3 in two amino acids. It has Alanine at positions 145 and 217.
Molecular Note This strain is a derivative of Tg(ACTB-Bgeo,-DsRed*MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. [MGI Ref ID J:109837]


Genotyping Information

Genotyping Protocols

Generic DsRed, QPCR
Generic DsRed, MELT
Generic DsRed, QPCR
Tg(DsRed), Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40(4):241-6. [PubMed: 15593332]  [MGI Ref ID J:109837]

Additional References

Tg(CAG-DsRed*MST)1Nagy related

Asai S; Otsuru S; Candela ME; Cantley L; Uchibe K; Hofmann TJ; Zhang K; Wapner KL; Soslowsky LJ; Horwitz EM; Enomoto-Iwamoto M. 2014. Tendon progenitor cells in injured tendons have strong chondrogenic potential: the CD105-negative subpopulation induces chondrogenic degeneration. Stem Cells 32(12):3266-77. [PubMed: 25220576]  [MGI Ref ID J:218549]

Avagyan S; Churchill M; Yamamoto K; Crowe JL; Li C; Lee BJ; Zheng T; Mukherjee S; Zha S. 2014. Hematopoietic stem cell dysfunction underlies the progressive lymphocytopenia in XLF/Cernunnos deficiency. Blood 124(10):1622-5. [PubMed: 25075129]  [MGI Ref ID J:214608]

Bald T; Quast T; Landsberg J; Rogava M; Glodde N; Lopez-Ramos D; Kohlmeyer J; Riesenberg S; van den Boorn-Konijnenberg D; Homig-Holzel C; Reuten R; Schadow B; Weighardt H; Wenzel D; Helfrich I; Schadendorf D; Bloch W; Bianchi ME; Lugassy C; Barnhill RL; Koch M; Fleischmann BK; Forster I; Kastenmuller W; Kolanus W; Holzel M; Gaffal E; Tuting T. 2014. Ultraviolet-radiation-induced inflammation promotes angiotropism and metastasis in melanoma. Nature 507(7490):109-13. [PubMed: 24572365]  [MGI Ref ID J:207458]

Birbrair A; Zhang T; Wang ZM; Messi ML; Mintz A; Delbono O. 2013. Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle. Am J Physiol Cell Physiol 305(11):C1098-113. [PubMed: 24067916]  [MGI Ref ID J:213084]

Charbel Issa P; Singh MS; Lipinski DM; Chong NV; Delori FC; Barnard AR; MacLaren RE. 2012. Optimization of in vivo confocal autofluorescence imaging of the ocular fundus in mice and its application to models of human retinal degeneration. Invest Ophthalmol Vis Sci 53(2):1066-75. [PubMed: 22169101]  [MGI Ref ID J:191520]

Cox BJ; Vollmer M; Tamplin O; Lu M; Biechele S; Gertsenstein M; van Campenhout C; Floss T; Kuhn R; Wurst W; Lickert H; Rossant J. 2010. Phenotypic annotation of the mouse X chromosome. Genome Res 20(8):1154-64. [PubMed: 20548051]  [MGI Ref ID J:163340]

Davey GM; Mueller SN; van Vliet C; Gigowski M; Zaid A; Davies B; Carbone FR; Heath WR. 2013. Identification of a MHC I-restricted epitope of DsRed in C57BL/6 mice. Mol Immunol 53(4):450-2. [PubMed: 23142929]  [MGI Ref ID J:191084]

Dong J; Huang S; Caikovski M; Ji S; McGrath A; Custorio MG; Creighton CJ; Maliakkal P; Bogoslovskaia E; Du Z; Zhang X; Lewis MT; Sablitzky F; Brisken C; Li Y. 2011. ID4 regulates mammary gland development by suppressing p38MAPK activity. Development 138(23):5247-56. [PubMed: 22069192]  [MGI Ref ID J:178679]

Dykstra B; Olthof S; Schreuder J; Ritsema M; de Haan G. 2011. Clonal analysis reveals multiple functional defects of aged murine hematopoietic stem cells. J Exp Med 208(13):2691-703. [PubMed: 22110168]  [MGI Ref ID J:179054]

Fan Z; Spencer JA; Lu Y; Pitsillides CM; Singh G; Kim P; Yun SH; Toxavidis V; Strom TB; Lin CP; Koulmanda M. 2010. In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response. Nat Med 16(6):718-22. [PubMed: 20495571]  [MGI Ref ID J:161525]

Flesken-Nikitin A; Hwang CI; Cheng CY; Michurina TV; Enikolopov G; Nikitin AY. 2013. Ovarian surface epithelium at the junction area contains a cancer-prone stem cell niche. Nature 495(7440):241-5. [PubMed: 23467088]  [MGI Ref ID J:194916]

Fu X; Zhao JX; Zhu MJ; Foretz M; Viollet B; Dodson MV; Du M. 2013. AMP-activated protein kinase alpha1 but not alpha2 catalytic subunit potentiates myogenin expression and myogenesis. Mol Cell Biol 33(22):4517-25. [PubMed: 24043309]  [MGI Ref ID J:206092]

Guarani V; Deflorian G; Franco CA; Kruger M; Phng LK; Bentley K; Toussaint L; Dequiedt F; Mostoslavsky R; Schmidt MH; Zimmermann B; Brandes RP; Mione M; Westphal CH; Braun T; Zeiher AM; Gerhardt H; Dimmeler S; Potente M. 2011. Acetylation-dependent regulation of endothelial Notch signalling by the SIRT1 deacetylase. Nature 473(7346):234-8. [PubMed: 21499261]  [MGI Ref ID J:172054]

He H; Xu J; Warren CM; Duan D; Li X; Wu L; Iruela-Arispe ML. 2012. Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages. Blood 120(15):3152-62. [PubMed: 22919031]  [MGI Ref ID J:192947]

Honda T; Egen JG; Lammermann T; Kastenmuller W; Torabi-Parizi P; Germain RN. 2014. Tuning of antigen sensitivity by T cell receptor-dependent negative feedback controls T cell effector function in inflamed tissues. Immunity 40(2):235-47. [PubMed: 24440150]  [MGI Ref ID J:209370]

Humphreys BD; Lin SL; Kobayashi A; Hudson TE; Nowlin BT; Bonventre JV; Valerius MT; McMahon AP; Duffield JS. 2010. Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. Am J Pathol 176(1):85-97. [PubMed: 20008127]  [MGI Ref ID J:158051]

Janzen DM; Cheng D; Schafenacker AM; Paik DY; Goldstein AS; Witte ON; Jaroszewicz A; Pellegrini M; Memarzadeh S. 2012. Estrogen and progesterone together expand murine endometrial epithelial progenitor cells. Stem Cells 31(4):808-22. [PubMed: 23341289]  [MGI Ref ID J:195174]

Jaras M; Miller PG; Chu LP; Puram RV; Fink EC; Schneider RK; Al-Shahrour F; Pena P; Breyfogle LJ; Hartwell KA; McConkey ME; Cowley GS; Root DE; Kharas MG; Mullally A; Ebert BL. 2014. Csnk1a1 inhibition has p53-dependent therapeutic efficacy in acute myeloid leukemia. J Exp Med 211(4):605-12. [PubMed: 24616378]  [MGI Ref ID J:210826]

Lammermann T; Afonso PV; Angermann BR; Wang JM; Kastenmuller W; Parent CA; Germain RN. 2013. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498(7454):371-5. [PubMed: 23708969]  [MGI Ref ID J:198733]

Lee JH; Bhang DH; Beede A; Huang TL; Stripp BR; Bloch KD; Wagers AJ; Tseng YH; Ryeom S; Kim CF. 2014. Lung Stem Cell Differentiation in Mice Directed by Endothelial Cells via a BMP4-NFATc1-Thrombospondin-1 Axis. Cell 156(3):440-55. [PubMed: 24485453]  [MGI Ref ID J:205555]

Li XM; Hu Z; Zafar AB; Jorgensen ML; Bungert J; Slayton W. 2010. Intrinsic and extrinsic effects of mafG deficiency on hematopoietic recovery following bone marrow transplant. Exp Hematol 38(12):1251-60. [PubMed: 20813153]  [MGI Ref ID J:169721]

Lin GH; Edele F; Mbanwi AN; Wortzman ME; Snell LM; Vidric M; Roth K; Hauser AE; Watts TH. 2012. Contribution of 4-1BBL on radioresistant cells in providing survival signals through 4-1BB expressed on CD8+ memory T cells in the bone marrow. Eur J Immunol 42(11):2861-74. [PubMed: 22886791]  [MGI Ref ID J:188780]

Mulholland DJ; Xin L; Morim A; Lawson D; Witte O; Wu H. 2009. Lin-Sca-1+CD49fhigh stem/progenitors are tumor-initiating cells in the Pten-null prostate cancer model. Cancer Res 69(22):8555-62. [PubMed: 19887604]  [MGI Ref ID J:154444]

Radulovic K; Rossini V; Manta C; Holzmann K; Kestler HA; Niess JH. 2013. The early activation marker CD69 regulates the expression of chemokines and CD4 T cell accumulation in intestine. PLoS One 8(6):e65413. [PubMed: 23776480]  [MGI Ref ID J:203306]

Santamaria X; Massasa EE; Taylor HS. 2012. Migration of cells from experimental endometriosis to the uterine endometrium. Endocrinology 153(11):5566-74. [PubMed: 22968642]  [MGI Ref ID J:191416]

Singh MS; Charbel Issa P; Butler R; Martin C; Lipinski DM; Sekaran S; Barnard AR; MacLaren RE. 2013. Reversal of end-stage retinal degeneration and restoration of visual function by photoreceptor transplantation. Proc Natl Acad Sci U S A 110(3):1101-6. [PubMed: 23288902]  [MGI Ref ID J:193719]

Starremans PG; Li X; Finnerty PE; Guo L; Takakura A; Neilson EG; Zhou J. 2008. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73(12):1394-405. [PubMed: 18385665]  [MGI Ref ID J:140012]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes or hemizygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   24-MAR-07
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Tg(CAG-DsRed*MST)1Nagy  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Tg(CAG-DsRed*MST)1Nagy x Homozygous for Tg(CAG-DsRed*MST)1Nagy  

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Tg(CAG-DsRed*MST)1Nagy  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Tg(CAG-DsRed*MST)1Nagy x Homozygous for Tg(CAG-DsRed*MST)1Nagy  

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

   000664 C57BL/6J
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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