| |||||||||||||||
| These Actb-DsRed.T3 transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer and may be useful for widespread fluorescent protein expression. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(ACTB-DsRed*MST)1Nagy/J (Changed: 08-MAY-08 ) B6.Cg-Tg(ACTB-DsRed.MST)1Nagy/J (Changed: 20-FEB-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 24-MAR-07 Species laboratory mouse Generation N5F6 (10-NOV-08) Donating Investigator Andras Nagy, Mount Sinai Hospital Description
Mice homozygous for this Actb-DsRed.T3 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description as published results become available.
Development
This strain is a derivative of Tg(ACTB-bgeo/DsRed.MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. ES cells containing the (ACTB-DsRed.MST) construct were aggregated with ICR outbred tetraploid embryos to generate chimeric mice. The resulting chimeric male animal was bred with an ICR outbred female. Progeny from this cross that were hemizygous for the transgene were bred to ICR outbred mice. The mice were then backcrossed to C57BL/6J for 5 generations. Of note, this "DsRed.MST" protein is erroneously designated "DsRed.T3" in the original publication.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
005441 STOCK Tg(CAG-DsRed*MST)1Nagy/J
008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J 009655 C57BL/6J-Tg(Dcx-DsRed)14Qlu/J 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J 005438 STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J 008241 STOCK Tg(Cspg4-DsRed.T1)1Akik/J 006866 STOCK Tg(Ins1-DsRed*T4)32Hara/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(CAG-DsRed*MST)1Nagy/0
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCr * ICR
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:109837)
- transgenic mice are viable and fertile
This mouse can be used to support research in many areas including:
DsRed relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-DsRed*MST)1Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andras Nagy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Actb-DsRed.T3; DsRed.T3; Tg(ACTB-DsRed*MST)1Nagy; Tg(ACTB-DsRed.MST)1Nagy; | ||
| Mutation Made By | Kristina Vintersten, Mount Sinai Hospital, SLRI | ||
| Strain of Origin | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| ES Cell Line Name | G4 | ||
| ES Cell Line Strain | (129S6/SvEvTac x C57BL/6NCr)F1 | ||
| Site of Expression | Widespread expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | This strain is a derivative of Tg(ACTB-Bgeo,-DsRed*MST). A transgenic construct containing a Red Fluorescent Protein gene (DsRed.MST) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer upstream of a loxP site flanked beta-geo gene and polyadenylation sites, was introduced into 129S6B6F1 derived G4 embryonic stem (ES) cells. Then the expression of lacZ was replaced with red fluorescent protein following in vitro Cre recombination. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. [MGI Ref ID J:109837] | ||
| Gene Symbol and Name | Tg(CAG-DsRed*MST)1Nagy, transgene insertion 1, Andras Nagy | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Actb-DsRed.T3; DsRed.T3; Tg(ACTB-DsRed*MST)1Nagy; Tg(ACTB-DsRed.MST)1Nagy; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic DsRed, Melt Curve Analysis
Generic DsRed, QPCR
Generic DsRed, Separated PCR
Generic DsRed, Standard PCR
Tg(DsRed), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Vintersten K; Monetti C; Gertsenstein M; Zhang P; Laszlo L; Biechele S; Nagy A. 2004. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40(4):241-6. [PubMed: 15593332] [MGI Ref ID J:109837]
Additional References
Tg(CAG-DsRed*MST)1Nagy relatedStarremans PG; Li X; Finnerty PE; Guo L; Takakura A; Neilson EG; Zhou J. 2008. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73(12):1394-405. [PubMed: 18385665] [MGI Ref ID J:140012]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes or hemizygotes. Mating System Homozygote x Homozygote (Female x Male) 24-MAR-07 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(CAG-DsRed*MST)1Nagy
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(CAG-DsRed*MST)1Nagy x Homozygous for Tg(CAG-DsRed*MST)1Nagy
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(CAG-DsRed*MST)1Nagy
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(CAG-DsRed*MST)1Nagy x Homozygous for Tg(CAG-DsRed*MST)1Nagy
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.