Strain Name: |
129-Gt(ROSA)26Sortm1Luo/J |
|---|---|
Stock Number: |
006053 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
129-Gt(ROSA)26Sortm1(EGFP)Luo/J (Changed: 31-OCT-06
) |
| Genes & Alleles | GFP; Gt(ROSA)26Sor; Gt(ROSA)26Sortm1Luo; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Liqun Luo, Stanford University, HHMI Generation ?+F4 (07-JUN-07) Strain Description
MADM-GG mice are viable with no gross behavioral or observable abnormalities. Regardless of Cre-recombination, these mice express EGFP as their N- and C-terminal coding sequences are interrupted by the beta-actin intron in-frame. High EGFP expression in every cell can be visualized in vivo and in fixed samples. These mutant mice are a control EGFP-expressing strain for use with MADM (mosaic analysis with double markers) mice (see Stock No. 006041 [MADM-GR (EGFP/Dsred2)] and Stock No. 006067 [MADM-RG (Dsred2/EGFP)]). Using the MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.Mice harboring this MADM-GG mutation are also available congenic on a C57BL/6J genetic background (see Stock No. 006071), providing a control strain for the C57BL/6J congenic MADM-GR (Stock No. 006075) and MADM-RG (Stock No. 006080) strains.
Strain Development
A targeting vector was designed to contain the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; Okada et al, Exp Neurol 1999 156:394-406), beta-globin intronic sequence (itself containing a loxP-flanked neomycin resistance gene), and remaining "in-frame" C-terminal portion of mut4-EGFP. This construct (GG) is preceded by the cytomegalovirus beta-actin enhancer-promoter and followed by the SV40 T antigen poly A signal, allowing for ubiquitous and high-level expression of the reporter genes. This entire construct was inserted into the Gt(ROSA)26Sor locus via electroporation of (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in C57BL/6 blastocysts and chimeric progeny were established. Mutant mice were bred to 129S1/SvImJ (Stock No. 002448) for one generation, and then intercrossed to homozygosity before arriving at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm1Luo | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Liqun Luo | ||
| Common Name(s) | GG; Gt(ROSA)26Sortm1(EGFP)Luo; MADM GG knockin; | ||
| Mutation Made By | Liqun Luo, Stanford University, HHMI | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | widespread expression; control strain for Stock No. 006067 and Stock No. 006041 | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A construct containing the CMV beta-actin enhancer/promoter, SV40 poly(A) signal, and encoding the N-terminal and C-terminal halves of a mutant enhanced green fluorescent protein (EGFP) separated by a beta-globin intron containing a loxP-flanked neomycinresistance gene, was knocked into ES cells at the ROSA26 locus. The two parts of the coding sequence are separated by the intron but still in the same reading frame. Ubiquitous GFP expression occurs in the absence of cre-mediated recombination. [J:98961] | ||
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Gt(ROSA)26Sortm1Luo, tm2Luo, tm3Luo, tm4ACTB-tdTomato,-EGFP)Luo
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
006071 B6.129-Gt(ROSA)26Sortm1Luo/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation
Research Tools
Cre-lox-System (loxP-flanked Sequences: Test/Reporter)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Genetics Research (Tissue/Cell Markers: Cre-lox system)
Genetics Research (Tissue/Cell Markers: multiple)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Zong H; Espinosa JS; Su HH; Muzumdar MD; Luo L. 2005. Mosaic analysis with double markers in mice. Cell 121(3):479-92. [PubMed: 15882628] [J:98961]
Price and Supply Information
| Strain Name: | 129-Gt(ROSA)26Sortm1Luo/J |
| Stock Number: | 006053 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $155.60 | Homozygous for Gt(ROSA)26Sortm1Luo | |||
| Pair | $311.20 | Homozygous for Gt(ROSA)26Sortm1Luo x Homozygous for Gt(ROSA)26Sortm1Luo | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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