Strain Name: |
B6.C-Tg(CMV-cre)1Cgn/J |
|---|---|
Stock Number: |
006054 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | cre; CMV; Tg(CMV-cre)1Cgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N5F2 (08-JAN-08) Strain Description
In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears that the cre gene is X-linked since transgene transmission through males is restricted to female offspring.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
The cre gene used in this mutant mouse was modified by adding a nuclear localization signal and the human growth hormone gene providing splicing and polyadenylation signals. A construct containing cre coding sequence under the control of a human cytomegalovirus minimal promoter was introduced to BALB/cJ derived BALB/c-I embryonic stem (ES) cells. The resulting mice were backcrossed to the BALB/c background for 8 generations and then backcrossed to the C57BL/6J background for 5 generations.
Gene & Allele Details
| Allele Symbol | Tg(CMV-cre)1Cgn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, University of Cologne | ||
| Common Name(s) | CMV-Cre; CMV:Cre; Cre deleter; TgN(CMV-Cre)#Cgn; deleter; ubi-cre; | ||
| Mutation Made By | Frieder Schwenk, University of Cologne | ||
| Strain of Origin | BALB/cJ | ||
| ES Cell Line Name | BALB/c-I | ||
| ES Cell Line Strain | BALB/cJ | ||
| Site of Expression | widespread expression, including germline | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | CMV, cytomegalovirus, human | ||
| General Note |
Homozygous transgenic mice are viable and fertile. The transgene appears to be X-linked since transgene transmission through males is restricted to female offspring. In transgenic mice, in conjunction with a transgene carrying a loxP-flanked gene, deletion of the loxP-flanked gene occurs in all tissues including germ cells. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a human cytomegalovirus (CMV) promoter, active in most cells and tissues. [J:75204] [J:92063] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. The cre transgene appears to be X-linked since transgene transmission through males is restricted to female offspring. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Tg(CMV-cre)1Cgn allele
003465 BALB/c-Tg(CMV-cre)1Cgn/J View Strains carrying Tg(CMV-cre)1Cgn (1 strain)
006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 006439 FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J 004375 FVB/N-Tg(tetO-Kras2)12Hev/J 002471 STOCK Tg(hCMV-cre)140Sau/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox-System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox system)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Genetics Research (Tissue/Cell Markers: Cre-lox system)
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesSchwenk F; Baron U; Rajewsky K. 1995. A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells. Nucleic Acids Res 23(24):5080-1. [PubMed: 8559668] [J:75204]
Price and Supply Information
| Strain Name: | B6.C-Tg(CMV-cre)1Cgn/J |
| Stock Number: | 006054 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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