Description

Strain Information

Former Names B6.CAST-(D2Mit405-D2Mit74)/LusJ    (Changed: 05-AUG-08 ) Type Congenic; Mutant Strain; Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse H2 Haplotype b

Appearance
black
Related Genotype: a/a

Description
This C57BL/6J congenic carries the proximal portion of chromosome 2 from CAST/Ei. The Mit or SNP (rs) markers in the strain name define the congenic interval.

Marker-assisted breeding was used to construct a set of overlapping congenic strains called genome-tagged mice (GTM) that span the entire mouse genome. Each member of the panel carries an average of a 23 cM segment (range 8 to 58 cM) from CAST/Ei introgressed on to a C57BL/6J background. The segments represent the distal (D), middle (M), and proximal (P) portions of all or most of a given chromosome. (Iakoubova OA, et. al., 2001) GTM are a valuable resource for mapping and confirmation of loci contributing to complex traits.

The Jackson Laboratory is working with Dr. Lusis' group to import and make available additional strains in the panel.

Development
This congenic panel was initiated by a cross of (CAST/Ei X C57BL/6)F1 females to C57BL/6J males. Male progeny at each generation were selected by marker assisted genome scan and were backcrossed to C57BL/6J females to produce the N3-N6 generations. All animals in the panel carry a C57BL/6J Y chromosome. Sibling pairs at the N6 generation were intercrossed to produce homozygotes. Iakoubova OA, et. al. provides a more detailed explanation of the chromosome intervals. This strain was imported to The Jackson Laboratory in 2006.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Genetics Research (Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis)

Genes & Alleles

Gene & Allele Information

Currently there are no related genes or alleles for this strain.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Iakoubova OA; Olsson CL; Dains KM; Ross DA; Andalibi A; Lau K; Choi J; Kalcheva I; Cunanan M; Louie J; Nimon V; Machrus M; Bentley LG; Beauheim C; Silvey S; Cavalcoli J; Lusis AJ; West DB. 2001. Genome-tagged mice (GTM): two sets of genome-wide congenic strains. Genomics 74(1):89-104. [PubMed: 11374905]  [MGI Ref ID J:69881]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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