Strain Name: |
STOCK Ptentm1Hwu/J |
|---|---|
Stock Number: |
006068 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6;129S4(C)-Ptentm1Hwu/J (Changed: 28-FEB-06
) |
| Genes & Alleles | Pten; Ptentm1Hwu; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N?+N1F6N1F5 (02-MAY-08) Strain Description
These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description as published results become available.
Strain Development
A loxP site flanked targeting vector containing hygromycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 5. This construct was electroporated into 129S4/SvJae derived LW-1 embryonic stem (ES) cells, which were transiently transfected with a Cre-recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/cAnNTac mice before being made homozygous. The mice were then backcrossed onto the C57BL/6J background for one generation and then made homozygous.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Ptentm1Hwu | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Hong Wu | ||
| Common Name(s) | Ptenfl; Ptenflox; Ptenloxp; | ||
| Mutation Made By | Hong Wu, UCLA | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | LW1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Pten, phosphatase and tensin homolog | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | 2310035O07Rik; A130070J02Rik; AI463227; BZS; MGC11227; MHAM; MMAC1; Mmac; PTEN1; RIKEN cDNA 2310035O07 gene; RIKEN cDNA A130070J02 gene; TEP1; expressed sequence AI463227; | ||
| Molecular Note | A loxP flanked hygromycin resistance cassette was inserted 5' to exon 5, and a single loxP site was inserted 3' to exon 5, which encodes the phosphatase domain. The hygromycin cassette was removed in ES cells by transient Cre recombinase expression prior to the production of chimeric mice, leaving a single loxP site in place of the cassette. These insertions do not appear to have any effect on the normal function of the gene. [MGI Ref ID J:75117] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Ptentm1Hwu
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Ptentm1Hwu allele
006440 B6.129S4-Ptentm1Hwu/J 004597 C;129S4-Ptentm1Hwu/J View Strains carrying Ptentm1Hwu (2 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Ptentm1Hwu relatedApoptosis Research
Endogenous Regulators
Cell Biology Research
Genes Regulating Growth and Proliferation
Research Tools
Apoptosis Research
Cancer Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Cancer Research
Tumor Suppressor Genes
Cell Biology Research
Cell Cycle Regulation
References
Selected Reference(s)
Additional ReferencesGroszer M; Erickson R; Scripture-Adams DD; Lesche R; Trumpp A; Zack JA; Kornblum HI; Liu X; Wu H. 2001. Negative regulation of neural stem/progenitor cell proliferation by the Pten tumor suppressor gene in vivo. Science 294(5549):2186-9. [PubMed: 11691952] [MGI Ref ID J:73255]
Price and Supply Information
| Strain Name: | STOCK Ptentm1Hwu/J |
| Stock Number: | 006068 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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