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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 17-MAY-08 Species laboratory mouse Generation ?+N5F9 (14-MAR-11)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an "H2B-EGFP" fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene [EGFP, BD Biosciences]) under the control of the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. Nucleosomes and chromatin in all cells fluoresce. Fluorescence is detectable during all phases of mitosis. The donating investigator reports occasional silencing of the transgene when transmitted through the female germline. This mutant mouse strain may be useful for in vivo subcellular high-resolution and 3-dimensional studies of dividing cells.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing an "H2B-EGFP" fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene [EGFP, BD Biosciences]) was placed under the control of a chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer promoter. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. H2B-EGFP expressing ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to 129/Tac mice for germline transmission. Resulting founder animals were bred with outbred ICR mice prior to arrival at The Jackson Laboratory. The mice were then backcrossed on to the C57BL/6J background for at least five generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
005418 STOCK Tg(HIST1H2BB/EGFP)1Pa/J
005418 STOCK Tg(HIST1H2BB/EGFP)1Pa/J 013752 STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J
005418 STOCK Tg(HIST1H2BB/EGFP)1Pa/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(HIST1H2BB/EGFP)1Pa/Tg(HIST1H2BB/EGFP)1Pa
involves: 129S1/Sv * 129X1/SvJ * ICR
- normal phenotype
- no abnormal phenotype detected
- homozygotes are viable and fertile; the reporter is developmentally neutral (MGI Ref ID J:109836)
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Cell Cycle Regulation
Transcriptional Regulation
Research Tools
Cell Biology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(HIST1H2BB/EGFP)1Pa | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Virginia E Papaioannou | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | CAG::H2B-EGFP; H2B-EGFP; HBb-EGFP; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Nucleosomes and chromatin | ||
| Expressed Gene | HIST1H2BB, histone cluster 1, H2bb, human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The coding sequence of EGFP was placed C-terminally to the coding sequence of the human histone H2B gene. When placed under control of the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer/promoter, a fluorescent fusion protein (H2B-EGFPis produced. [MGI Ref ID J:109836] | ||
| Gene Symbol and Name | Tg(HIST1H2BB/EGFP)1Pa, transgene insertion 1, Virginia E Papaioannou | ||
| Chromosome | UN | ||
| Gene Common Name(s) | CAG::H2B-EGFP; H2B-EGFP; HBb-EGFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hadjantonakis AK; Papaioannou VE. 2004. Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice. BMC Biotechnol 4:33. [PubMed: 15619330] [MGI Ref ID J:109836]
Additional References
Tg(HIST1H2BB/EGFP)1Pa relatedWyngaarden LA; Vogeli KM; Ciruna BG; Wells M; Hadjantonakis AK; Hopyan S. 2010. Oriented cell motility and division underlie early limb bud morphogenesis. Development 137(15):2551-8. [PubMed: 20554720] [MGI Ref ID J:168374]
Zhao J; Mo V; Nagasaki T. 2009. Distribution of label-retaining cells in the limbal epithelium of a mouse eye. J Histochem Cytochem 57(2):177-85. [PubMed: 19001638] [MGI Ref ID J:146975]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred wildtype siblings. For mice on the STOCK background (Stock No. 005418), the donating investigator reports occasional silencing of the transgene when transmitted through the female germ line and recommends identifying mutant offspring by the fluorescent phenotype. Mating System +/+ sibling x Hemizygote (Female x Male) 17-MAY-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(HIST1H2BB/EGFP)1Pa
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(HIST1H2BB/EGFP)1Pa x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(HIST1H2BB/EGFP)1Pa Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(HIST1H2BB/EGFP)1Pa
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(HIST1H2BB/EGFP)1Pa x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(HIST1H2BB/EGFP)1Pa Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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