Strain Name:

CBy.129S6(B6)-Epha2tm1Jrui/J

Stock Number:

006070

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C.129S6(B6)-Epha2tm1Jrui/J    (Changed: 28-FEB-06 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice homozygous for this targeted mutation are viable, fertile, and display no overt developmental or behavioral abnormalities. In mutant mice of a mixed BALB/c, C57BL/6, and 129S6 background, murine pulmonary microvascular endothelial cells (MPMECs) isolated from homozygotes express no endogenous protein. MPMECs from Epha2-deficient mice show impaired ephrin-A1-induced vascular assembly and defective migration both in vitro and in vivo. In addition, MPMECs from homozygous Epha2 mice exhibit decreased angiogenesis and fail to activate Rac1 in response to ephrin-A1 in vivo. Mutant mice on a BALB/c background that were transplanted with metastatic mammary adenocarcinoma cells showed impaired tumor progression, angiogenesis and metastasis to the lung, compared with wildtype littermate controls. These mutant mice may be useful in studies of postnatal angiogenesis (endothelial cell migration, assembly into new tubules, and cytoskeletal regulation), or as a tool to study cancer, especially breast cancer research.

Development
A targeting vector containing the neomycin cassette was used to disrupt exon 5 (at nucleotide 1372, corresponding to amino acid residue 426 in the extracellular domain) of the endogenous Epha2 gene. This construct was microinjected into 129S6/SvEvTac-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were bred to C57BL/6 females for one generation and backcrossed to BALB/c once before being interbred to homozygosity. Mutant null mice were maintained by sibling matings for more than 10 years prior to arrival at The Jackson Laboratory. The mice were then backcrossed on to the BALB/cByJ (Stock No. 001026) background for 5 generations.

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Epha2tm1Jrui allele
006028   B6;129S6-Epha2tm1Jrui/J
View Strains carrying   Epha2tm1Jrui     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Cataract 6, Multiple Types; CTRCT6   (EPHA2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Epha2tm1Jrui/Epha2tm1Jrui

        involves: 129 * BALB/c * C57BL/6
  • cardiovascular system phenotype
  • abnormal angiogenesis
    • endothelial cells fail to assemble into capillary-like structure (vascular assembly) and to migrate in response to ephrin A1 (Efna1) in vitro   (MGI Ref ID J:89275)
    • mutants show impaired ephrin A1-induced angiogenesis in vivo   (MGI Ref ID J:89275)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Other
      tumor metastasis
Tumor Resistance
Tumor Suppressor Genes

Cardiovascular Research
Vascular Defects

Research Tools
Cancer Research
      anti-tumor activity
      tumor immunology
Cardiovascular Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Epha2tm1Jrui
Allele Name targeted mutation 1, Joseph C Ruiz
Allele Type Targeted (Null/Knockout)
Common Name(s) Epha2-; Epha2tm1Jinc;
Mutation Made By Joseph Ruiz,   Indiana University School of Medicine
Strain of Origin129S/SvEv-Gpi1
ES Cell Line NameCCE/EK.CCE
ES Cell Line Strain129S/SvEv-Gpi1
Gene Symbol and Name Epha2, Eph receptor A2
Chromosome 4
Gene Common Name(s) ARCC2; AW545284; CTPA; CTPP1; CTRCT6; ECK; Eck; Myk2; Sek-2; Sek2; epithelial cell kinase; expressed sequence AW545284;
Molecular Note A neo cassette was inserted at nucleotide 1372, within the sequence encoding the extracellular domain. Although the resultant transcript putatively encodes a nonfunctional protein truncated at amino acid 426, no protein was detected by Western blot analysis of murine pulmonary microvascular endothelial cells from mutant mice. [MGI Ref ID J:89275]

Genotyping

Genotyping Information

Genotyping Protocols

Epha2tm1Jrui, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Brantley-Sieders DM; Caughron J; Hicks D; Pozzi A; Ruiz JC; Chen J. 2004. EphA2 receptor tyrosine kinase regulates endothelial cell migration and vascular assembly through phosphoinositide 3-kinase-mediated Rac1 GTPase activation. J Cell Sci 117(Pt 10):2037-49. [PubMed: 15054110]  [MGI Ref ID J:89275]

Additional References

Epha2tm1Jrui related

Brantley-Sieders DM; Fang WB; Hicks DJ; Zhuang G; Shyr Y; Chen J. 2005. Impaired tumor microenvironment in EphA2-deficient mice inhibits tumor angiogenesis and metastatic progression. FASEB J 19(13):1884-6. [PubMed: 16166198]  [MGI Ref ID J:102664]

Brantley-Sieders DM; Zhuang G; Hicks D; Fang WB; Hwang Y; Cates JM; Coffman K; Jackson D; Bruckheimer E; Muraoka-Cook RS; Chen J. 2008. The receptor tyrosine kinase EphA2 promotes mammary adenocarcinoma tumorigenesis and metastatic progression in mice by amplifying ErbB2 signaling. J Clin Invest 118(1):64-78. [PubMed: 18079969]  [MGI Ref ID J:130805]

Carpenter TC; Schroeder W; Stenmark KR; Schmidt EP. 2012. Eph-A2 promotes permeability and inflammatory responses to bleomycin-induced lung injury. Am J Respir Cell Mol Biol 46(1):40-7. [PubMed: 21799118]  [MGI Ref ID J:191913]

Cheng C; Ansari MM; Cooper JA; Gong X. 2013. EphA2 and Src regulate equatorial cell morphogenesis during lens development. Development 140(20):4237-45. [PubMed: 24026120]  [MGI Ref ID J:204508]

Hahn AS; Kaufmann JK; Wies E; Naschberger E; Panteleev-Ivlev J; Schmidt K; Holzer A; Schmidt M; Chen J; Konig S; Ensser A; Myoung J; Brockmeyer NH; Sturzl M; Fleckenstein B; Neipel F. 2012. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus. Nat Med 18(6):961-6. [PubMed: 22635007]  [MGI Ref ID J:186781]

Okazaki T; Ni A; Baluk P; Ayeni OA; Kearley J; Coyle AJ; Humbles A; McDonald DM. 2009. Capillary defects and exaggerated inflammatory response in the airways of EphA2-deficient mice. Am J Pathol 174(6):2388-99. [PubMed: 19443703]  [MGI Ref ID J:148922]

Rao A; Taylor JL; Chi-Sabins N; Kawabe M; Gooding WE; Storkus WJ. 2012. Combination therapy with HSP90 inhibitor 17-DMAG reconditions the tumor microenvironment to improve recruitment of therapeutic T cells. Cancer Res 72(13):3196-206. [PubMed: 22552283]  [MGI Ref ID J:189322]

Shi Y; De Maria A; Bennett T; Shiels A; Bassnett S. 2012. A role for epha2 in cell migration and refractive organization of the ocular lens. Invest Ophthalmol Vis Sci 53(2):551-9. [PubMed: 22167091]  [MGI Ref ID J:191524]

Vaught D; Chen J; Brantley-Sieders DM. 2009. Regulation of mammary gland branching morphogenesis by EphA2 receptor tyrosine kinase. Mol Biol Cell 20(10):2572-81. [PubMed: 19321667]  [MGI Ref ID J:175142]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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