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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.129-Gt(ROSA)26Sortm1(EGFP/Dsred2)Luo/J (Changed: 01-DEC-06 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N5F2pN1
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
MADM-GR mice are viable with no gross behavioral or observable abnormalities. Homozygous mice have low fertility, while heterozygous mice have no reported fertility defects. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-RG (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recombinase introduction that facilitates inter-chromosomal recombination aligns the respective N- and C-terminal coding sequences for each of the reporter genes on the same chromosome. The following chromatid segregation (X or Z) determines daughter cell phenotype (recombinant sister chromatids into the same daughter cell leads to double reporter expression [a G2-Z event], while independent segregation into separate daughter cells leads to expression of EGFP or Dsred2-MYC [a G2-X event]). If an additional targeted mutation of interest is introduced distal to the Gt(ROSA) locus, only homozygous cells will be singly labeled following G2 Cre-introduction. The homozygous mutant and wild type cells can then be distinguished by which single reporter they express. Reporter protein tissue specificity, expression levels, and frequency of recombination are thus determined by the promoter controlling Cre expression. Using this MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.Mice harboring this MADM-GR mutation are also available on their original 129 genetic background (see Stock No. 006041). A control strain (Stock No. 006071, MADM-GG (EGFP/EGFP)) is also provided in which EGFP is in-frame on the same chromosome. Other important features of the MADM system are listed below. Cre-recombinase introduction in cell phase G0 or G1 results in double reporter expression. While EGFP expression can be visualized in vivo and in fixed samples, Dsred2-MYC can not. Anti-MYC immunofluorescence allows simultaneous visualization of both reporter genes. Single copy Dsred2-MYC is inadequate for live visualization.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector was designed to contain the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; Okada et al, Exp Neurol 1999 156:394-406), beta-globin intronic sequence (itself containing a loxP-flanked neomycin resistance gene), and C-terminal portion of a red fluorescent protein (Dsred2; Clonetech) tagged with six copies of the MYC epitope at its C-terminus. This construct (GR) is preceded by the cytomegalovirus beta-actin enhancer-promoter and followed by the SV40 T antigen poly A signal, allowing for ubiquitous and high-level expression of the reporter genes. This entire construct was inserted into the Gt(ROSA)26Sor locus via electroporation of (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in C57BL/6 blastocysts and chimeric progeny were established. Mutant mice were bred to 129S1/SvImJ (Stock No. 002448) for at least three generations, and then intercrossed to homozygosity before arriving at The Jackson Laboratory (as Stock No. 006041). Upon arrival, mice were backcrossed to C57BL/6J for at least 5 generations to establish this colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 006071 B6.129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
006041 129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo
involves: 129S1/Sv * 129X1/SvJThis mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: multiple
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Liqun Luo | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | GR; Gt(ROSA)26Sortm1(EGFP/Dsred2)Luo; Gt(ROSA)26Sortm3Luo; MADM GR knockin; | ||
| Mutation Made By | Dr. Liqun Luo, Stanford University, HHMI | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | when crossed to reciprocal strain (Stock No. 006067) and then to a cre strain, differential expression of the reporter protein(s) will be expressed in daughter cells, depending on chromatid segregation | ||
| Expressed Gene | RFP, Red Fluorescent Protein, coral | ||
| Red Fluorescent Protein (RFP), derived from marine invertebrate organisms such as the soft coral Discosoma spp and reef coral, Heteractis crispa, is a versatile reporter molecule which has found use in many biological applications. The wild type protein, which is an obligate tetramer, is not well tolerated in mammalian systems. The original molecule has been modified in order to optimize expression to mammalian physiology (examples include monomeric RFP, mRFP1, DsRed, etc). | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | A construct containing the CMV beta-actin enhancer/promoter, SV40 poly(A) signal, and encoding the N-terminal part of EGFP and C-terminal part of Dsred2 (red fluorescent protein; also tagged with 6 copies of the MYC epitope) separated by a beta-globin intron containing a loxP-flanked neomycin resistance gene, was knocked into ES cells at the ROSA26 locus. No protein is expressed from the chimeric locus in absence of cre-mediated recombination because the coding sequences are interrupted by the intron in different reading frames. After recombination with the reciprocal mutation at the same locus carried by another mouse line, which restores the reading frames of EGFP and RFP, RNA splicing will remove the loxP sites, resulting in functional protein expression. [MGI Ref ID J:98961] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor(Luo),Separated MCA
Gt(ROSA)26Sortm1Luo, tm2Luo, tm3Luo, tm4ACTB-tdTomato,-EGFP)Luo, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Zong H; Espinosa JS; Su HH; Muzumdar MD; Luo L. 2005. Mosaic analysis with double markers in mice. Cell 121(3):479-92. [PubMed: 15882628] [MGI Ref ID J:98961]
Additional References
Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo relatedBonaguidi MA; Wheeler MA; Shapiro JS; Stadel RP; Sun GJ; Ming GL; Song H. 2011. In vivo clonal analysis reveals self-renewing and multipotent adult neural stem cell characteristics. Cell 145(7):1142-55. [PubMed: 21664664] [MGI Ref ID J:173371]
Desgraz R; Herrera PL. 2009. Pancreatic neurogenin 3-expressing cells are unipotent islet precursors. Development 136(21):3567-74. [PubMed: 19793886] [MGI Ref ID J:153962]
Fuerst PG; Bruce F; Rounds RP; Erskine L; Burgess RW. 2012. Cell autonomy of DSCAM function in retinal development. Dev Biol 361(2):326-37. [PubMed: 22063212] [MGI Ref ID J:179393]
Luo L. 2006. Gt(ROSA)26Sor<tm3Luo>- a marker for mosaic analysis MGI Direct Data Submission :. [MGI Ref ID J:113339]
Muzumdar MD; Luo L; Zong H. 2007. Modeling sporadic loss of heterozygosity in mice by using mosaic analysis with double markers (MADM). Proc Natl Acad Sci U S A 104(11):4495-500. [PubMed: 17360552] [MGI Ref ID J:120052]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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