Strain Name: |
B6.129P2(Cg)-Foxg1tm1(cre)Skm/J |
|---|---|
Stock Number: |
006084 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Foxg1; Foxg1tm1(cre)Skm; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N6 (02-MAY-08) Strain Description
This strain expresses Cre recombinase from the endogenous Foxg1 locus. Forkhead box G1 is required for telencephalon development and is expressed specifically in the telencephalon and discrete head structures. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in the telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. Mice that are homozygous for the targeted mutation die perinatally. Heterozygous mutant mice are viable, fertile, normal in size. On the C57BL/6 background, forebrain volume in heterozygotes is substantially reduced especially in the cerebral cortex (40.7%), striatum (29.7%), and hippocampus (18.6%). In the adult, the thalamus is reduced in volume by 21.6%. This mutant mouse strain represents a model that may be useful in studies of telencephalic development.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector containing cre coding sequence, neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt most of the coding sequence the targeted gene. The endogenous Foxg1 promoter drives expression of the Cre recombinase through the in-frame insertion of the cre coding sequence to the first 13 codons of the Foxg1 gene. The construct was electroporated into 129P2/OlaHsd-Hprtb-m1 derived HM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were initially backcrossed to Swiss Webster mice, then backcrossed for 9 generations on a 129 background and then backcrossed for 5 generations on to the C57BL/6J background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Foxg1tm1(cre)Skm | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Susan K McConnell | ||
| Common Name(s) | Foxg1-; Foxg1-Cre; Foxg1:Cre; Foxg1Cre; TgH(Foxg1-Cre)1Skm; | ||
| Mutation Made By | Susan McConnell, Stanford University | ||
| Strain of Origin | 129P2/OlaHsd-Hprt1 | ||
| ES Cell Line Name | HM-1 | ||
| ES Cell Line Strain | 129P2/OlaHsd-Hprt1 | ||
| Site of Expression | telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Foxg1, forkhead box G1 | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | 2900064B05Rik; BF-1; BF1; BF1A; BF2; FHKL3; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C; HBF-1; HBF-2; HBF-3; HBF-G2; HBF2; HFK1; HFK2; HFK3; HNF-3/forkhead homolog, brain factor 1; Hfh9; Hfhbf1; KHL2; QIN; RATBF1A; RIKEN cDNA 2900064B05 gene; | ||
| General Note | Cre mediated recombination was demonstrated in 3 reporter mouse lines in the following tissues: telencephalon, anterior optic vesicle, otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction, and pharyngeal pouches. | ||
| Molecular Note | Most of the coding region was replaced with a cre gene and a neomycin cassette. The cre gene sequence was fused in-frame following the first 13 codons of the gene. The endogenous promoter is active in telencephalon, anterior optic vesicle, otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. [MGI Ref ID J:62916] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Foxg1tm1(cre)Skm
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as heterozygotes. |
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Related Strains
Strains carrying Foxg1tm1(cre)Skm allele
004337 129.Cg-Foxg1tm1(cre)Skm/J View Strains carrying Foxg1tm1(cre)Skm (1 strain)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Genetics Research (Tissue/Cell Markers: astrocytes, neurons)
Neurobiology Research
Foxg1tm1(cre)Skm relatedResearch Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Developmental Biology Research
Cell Motility Defects
Craniofacial and Palate Defects
Defects in Cell Adhesion Molecules
Defects in Extracellular Matrix Molecules
Neural Crest Defects
Neural Tube Defects
Neurodevelopmental Defects
Neurobiology Research
Astrocyte Defects
Cortical Defects
Neural Tube Defects
Neurodevelopmental Defects
Neurotrophic Factor Defects
Vestibular and Hearing Defects
Sensorineural Research
Eye Defects
Olfactory Defects
Retinal Degeneration
Vestibular and Hearing Defects
References
Selected Reference(s)
Additional ReferencesEagleson KL; Schlueter McFadyen-Ketchum LJ; Ahrens ET; Mills PH; Does MD; Nickols J; Levitt P. 2007. Disruption of Foxg1 expression by knock-in of cre recombinase: effects on the development of the mouse telencephalon. Neuroscience 148(2):385-99. [PubMed: 17640820] [MGI Ref ID J:128207]
Hebert JM; McConnell SK. 2000. Targeting of cre to the Foxg1 (BF-1) locus mediates loxP recombination in the telencephalon and other developing head structures. Dev Biol 222(2):296-306. [PubMed: 10837119] [MGI Ref ID J:62916]
Price and Supply Information
| Strain Name: | B6.129P2(Cg)-Foxg1tm1(cre)Skm/J |
| Stock Number: | 006084 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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