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Strain Name:

B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J

Stock Number:

006098

Availability:

Repository- Live


General Terms and Conditions

Genes & Alleles   Il2;   Nfatc2;   luc;   Tg(Il2/NFAT-luc)83Rinc;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHomozygote x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating InvestigatorDr. Mercedes Rincon,   The University of Vermont
GenerationN10+N1F2 (31-JUL-07)

Strain Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygous females are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for NFAT, an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These mice may be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation.

Strain Development
A targeting vector containing the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Transgenic offspring (founder line 83) were crossed with “B10.BR/SgSnJ (H2-K)” (see Stock No. 000465) wildtype mice for 5 generations, and then backcrossed to C57BL/6 for more than 10 generations before arriving at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol Tg(Il2/NFAT-luc)83Rinc
Allele Name transgene insertion 83, Mercedes Rincon
Common Name(s) NFAT-luc;
Mutation Made ByDr. Mercedes Rincon,   The University of Vermont
Strain of Origin(C57BL/6 x SJL)F2
Site of ExpressionLuciferase activity in these transgenic mice identifies NFAT-mediated transcription. NFAT is an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression.
Expressed Gene luc, luciferase, firefly
Promoter Il2, interleukin 2, mouse, laboratory
Promoter Nfatc2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, mouse, laboratory
Molecular Note A transgene construct was designed with the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 (IL-2) minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. [J:106466]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as hemizygotes. The donating investigator reports that homozygous females in their colony are subfertile.
Diet Information LabDiet® 5K52/5K67

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View Fluorescent Protein Strains     (135 strains)

Strains carrying   Tg(Il2/NFAT-luc)83Rinc allele
006102   B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
View Strains carrying   Tg(Il2/NFAT-luc)83Rinc     (1 strain)

Strains carrying other alleles of Il2
002252   B6.129P2-Il2tm1Hor/J
002229   C.129P2(B6)-Il2tm1Hor/J
002228   C3.129P2(B6)-Il2tm1Hor/J
002573   NOD.129P2(B6)-Il2tm1Hor/DvsJ
View Strains carrying other alleles of Il2     (4 strains)

View Strains carrying other alleles of luc     (8 strains)

Additional Web Information

Congenic Nomenclature
Fluorescent Proteins/lacZ Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction
Transcriptional Regulation

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Reproductive Biology Research
Fertility Defects

Research Tools
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Genetics Research (Tissue/Cell Markers: fos/luc)

References

Selected Reference(s)

Rincon M; Flavell RA. 1996. Regulation of AP-1 and NFAT transcription factors during thymic selection of T cells. Mol Cell Biol 16(3):1074-84. [PubMed: 8622652]  [J:106463]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J
Stock Number: 006098

Price Details

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Supply Details

Standard SupplyRepository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of this STRAIN, including cross-bred progeny, by for-profit entities or for Commercial Purposes requires a license prior to shipping

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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