Strain Name: |
B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J |
|---|---|
Stock Number: |
006098 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Il2; Nfatc2; luc; Tg(Il2/NFAT-luc)83Rinc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Dr. Mercedes Rincon, The University of Vermont Generation N10+N1F2 (31-JUL-07) Strain Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygous females are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for NFAT, an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These mice may be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation.Strain Development
A targeting vector containing the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Transgenic offspring (founder line 83) were crossed with “B10.BR/SgSnJ (H2-K)” (see Stock No. 000465) wildtype mice for 5 generations, and then backcrossed to C57BL/6 for more than 10 generations before arriving at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Il2/NFAT-luc)83Rinc | ||
|---|---|---|---|
| Allele Name | transgene insertion 83, Mercedes Rincon | ||
| Common Name(s) | NFAT-luc; | ||
| Mutation Made By | Dr. Mercedes Rincon, The University of Vermont | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. NFAT is an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Il2, interleukin 2, mouse, laboratory | ||
| Promoter | Nfatc2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, mouse, laboratory | ||
| Molecular Note | A transgene construct was designed with the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 (IL-2) minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. [J:106466] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. The donating investigator reports that homozygous females in their colony are subfertile. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
002252 B6.129P2-Il2tm1Hor/J 002229 C.129P2(B6)-Il2tm1Hor/J 002228 C3.129P2(B6)-Il2tm1Hor/J 002573 NOD.129P2(B6)-Il2tm1Hor/DvsJ
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 005999 B6(SJL)-Tg(SBE/Tk-luc)7Twc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Transcriptional Regulation
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Reproductive Biology Research
Fertility Defects
Research Tools
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Genetics Research (Tissue/Cell Markers: fos/luc)
References
Selected Reference(s)
Additional ReferencesRincon M; Flavell RA. 1996. Regulation of AP-1 and NFAT transcription factors during thymic selection of T cells. Mol Cell Biol 16(3):1074-84. [PubMed: 8622652] [J:106463]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J |
| Stock Number: | 006098 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of this STRAIN, including cross-bred progeny, by for-profit entities or for Commercial Purposes requires a license prior to shipping
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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