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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Hemizygote (Female x Male) 27-MAR-08 Species laboratory mouse Generation N10+N1F2 (31-JUL-07) Donating Investigator Dr. Mercedes Rincon, The University of Vermont Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygous females are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for NFAT, an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These mice may be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation.Development
A targeting vector containing the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Transgenic offspring (founder line 83) were crossed with “B10.BR/SgSnJ (H2-K)” (see Stock No. 000465) wildtype mice for 5 generations, and then backcrossed to C57BL/6 for more than 10 generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
002252 B6.129P2-Il2tm1Hor/J 002229 C.129P2(B6)-Il2tm1Hor/J 002228 C3.129P2(B6)-Il2tm1Hor/J 000486 MRL/MpJ 002573 NOD.129P2(B6)-Il2tm1Hor/DvsJ 003852 NOD.B6-Il2C57BL/6/Lt 001289 NOD/ShiLt 001976 NOD/ShiLtJ 000686 SJL/J
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 005999 B6(SJL)-Tg(SBE/Tk-luc)7Twc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006851 B6.Cg-Tg(Per1-luc)025Jt/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J 009638 FVB-Tg(GFAP-luc,GAPDH-rluc)172.9Mes/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 002060 FVB/N-Tg(HIV-luc)326Morr/J 007800 FVB/N-Tg(Ins1-luc)VUPwrs/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Transcriptional Regulation
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Reproductive Biology Research
Fertility Defects
Research Tools
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: transcriptional activation
Tissue/Cell Markers: fos/luc
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Il2/NFAT-luc)83Rinc | ||
|---|---|---|---|
| Allele Name | transgene insertion 83, Mercedes Rincon | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | NFAT-luc; | ||
| Mutation Made By | Dr. Mercedes Rincon, The University of Vermont | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. NFAT is an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Nfatc2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, mouse, laboratory | ||
| Promoter | Il2, interleukin 2, mouse, laboratory | ||
| Molecular Note | A transgene construct was designed with the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 (IL-2) minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. [MGI Ref ID J:106466] | ||
| Gene Symbol and Name | Tg(Il2/NFAT-luc)83Rinc, transgene insertion 83, Mercedes Rincon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | NFAT-luc; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Il2/NFAT-luc)83Rinc, QPCR
Tg(luc), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Rincon M; Flavell RA. 1996. Regulation of AP-1 and NFAT transcription factors during thymic selection of T cells. Mol Cell Biol 16(3):1074-84. [PubMed: 8622652] [MGI Ref ID J:106463]
Additional References
Tg(Il2/NFAT-luc)83Rinc relatedLeung-Theung-Long S; Mondor I; Guiraud M; Lamare C; Nageleekar V; Paulet PE; Rincon M; Guerder S. 2009. Impaired NFAT transcriptional activity in antigen-stimulated CD8 T cells linked to defective phosphorylation of NFAT transactivation domain. J Immunol 182(11):6807-14. [PubMed: 19454676] [MGI Ref ID J:148865]
Rincon M; Flavell RA. 1997. Transcription mediated by NFAT is highly inducible in effector CD4+ T helper 2 (Th2) cells but not in Th1 cells. Mol Cell Biol 17(3):1522-34. [PubMed: 9032280] [MGI Ref ID J:106462]
Verweij CL; Guidos C; Crabtree GR. 1990. Cell type specificity and activation requirements for NFAT-1 (nuclear factor of activated T-cells) transcriptional activity determined by a new method using transgenic mice to assay transcriptional activity of an individual nuclear factor. J Biol Chem 265(26):15788-95. [PubMed: 2394747] [MGI Ref ID J:106466]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. The donating investigator reports that homozygous females in their colony are subfertile. Mating System Homozygote x Hemizygote (Female x Male) 27-MAR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Il2/NFAT-luc)83Rinc $300.70 Female or Male Homozygous for Tg(Il2/NFAT-luc)83Rinc
Pairs /Price (US dollars $) Pair Genotype $544.20 Hemizygous for Tg(Il2/NFAT-luc)83Rinc x Homozygous for Tg(Il2/NFAT-luc)83Rinc
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Il2/NFAT-luc)83Rinc $391.00 Female or Male Homozygous for Tg(Il2/NFAT-luc)83Rinc
Pairs /Price (US dollars $) Pair Genotype $707.50 Hemizygous for Tg(Il2/NFAT-luc)83Rinc x Homozygous for Tg(Il2/NFAT-luc)83Rinc
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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