Strain Name: |
B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J |
|---|---|
Stock Number: |
006100 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Fos; H2; H2k; luc; Tg(NFkB/Fos-luc)26Rinc; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Major Histocompatibility Congenic Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Dr. Mercedes Rincon, The University of Vermont Generation +N1PF5 (01-MAY-08) Strain Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mutant mice have the luciferase gene driven by two copies of the NF-kappaB (NF-kB or NFkB) regulatory element. The presence of nuclear NF-kB DNA binding activity (as detected by electrophoretic mobility shift assay [EMSA]) is consistent with luciferase reporter activity; these reporter mice identify NF-kB transcriptional activity in any tissue. These transgenic mice may be useful in studies of immunology, cellular signaling, signal transduction, apoptosis, and transcription factor function.Strain Development
The pBIIX-luciferase (pBIIX-luc) targeting vector contains the firefly luciferase gene (luc) driven by two NF-kB binding sites from the kappa light chain enhancer in front of a minimal fos promoter. This construct was microinjected into (C57BL/6 x SJL)F2 embryos. Transgenic offspring (founder line 26) were crossed with “B10.BR/SgSnJ (H2-K)” mice (see Stock No. 000465) for approximately 20 generations before arriving at The Jackson Laboratory.
| Allele Symbol | H2k | ||
|---|---|---|---|
| Allele Name | k variant | ||
| Common Name(s) | H-2k; | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note | The k variant has been observed in the following strains: C3H, C3H/HeJ, C3H/An, C3H/Bi, C3H/DiSn, C3H/St, CBA, CBA/CaJ, CBA/N, AKR/J, CE/J, C57BR/cdJ, C58/J, MA/J, RF/J, ST/bJ, MRL/MpJ-Faslpr/J | ||
| Allele Symbol | Tg(NFkB/Fos-luc)26Rinc | ||
| Allele Name | transgene insertion 26, Mercedes Rincon | ||
| Mutation Made By | Dr. Mercedes Rincon, The University of Vermont | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | Luciferase reporter activity identifies NF-kB transcriptional activity in any tissue. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Fos, FBJ osteosarcoma oncogene, mouse, laboratory | ||
| Molecular Note | The pBIIX-luciferase (pBIIX-luc) transgenic construct contains the firefly luciferase gene (luc) driven by two NF-kB binding sites from the kappa light chain enhancer in front of a minimal fos promoter. [MGI Ref ID J:106461] [MGI Ref ID J:106465] | ||
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000465 B10.BR-H2k H2-T18a/SgSnJ | ||
| 004804 B10.BR-H2k H2-T18a/SgSnJJrep | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Tg(luc)
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Fluorescent Protein Strains
View Fluorescent Protein Strains (146 strains)
Strains carrying H2k allele
000465 B10.BR-H2k H2-T18a/SgSnJ 004804 B10.BR-H2k H2-T18a/SgSnJJrep 006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J 001148 B6.AK-H2k/FlaEgJ 001895 B6.AK-H2k/J 001951 C.C3-H2k/LilMcdJ 001383 LT.MA-Glo1b H2k/J View Strains carrying H2k (7 strains)
Strains carrying other alleles of Fos
002099 B6.129X1-Fostm1Pa/J 002293 B6;129X1-Fostm1Pa/J View Strains carrying other alleles of Fos (2 strains)
Strains carrying other alleles of H2
View Strains carrying other alleles of H2 (51 strains)
Strains carrying other alleles of luc
006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J 005999 B6(SJL)-Tg(SBE/Tk-luc)7Twc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 002060 FVB/N-Tg(HIV-luc)326Morr/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J View Strains carrying other alleles of luc (10 strains)
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Room Number AX11
H2k relatedCell Biology Research
Signal Transduction
Transcriptional Regulation
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Research Tools
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Genetics Research (Tissue/Cell Markers: fos/luc)
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Selected Reference(s)
Additional ReferencesVoll RE; Jimi E; Phillips RJ; Barber DF; Rincon M; Hayday AC; Flavell RA; Ghosh S. 2000. NF-kappa B activation by the pre-T cell receptor serves as a selective survival signal in T lymphocyte development. Immunity 13(5):677-89. [PubMed: 11114380] [MGI Ref ID J:106461]
| Strain Name: | B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J |
| Stock Number: | 006100 |
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(NFkB/Fos-luc)26Rinc | |||
| Pair | $288.65 | Hemizygous for Tg(NFkB/Fos-luc)26Rinc x Noncarrier | |||
| Pair | $288.65 | Noncarrier x Hemizygous for Tg(NFkB/Fos-luc)26Rinc | |||
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
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