Strain Name: |
B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J |
|---|---|
Stock Number: |
006101 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(AP1/Prl-luc)31FlvRinc/J (Changed: 26-APR-06
) |
| Genes & Alleles | Prl; luc; Tg(TRE/Prl-luc)31FlvRinc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Dr. Mercedes Rincon, The University of Vermont Generation N15p+1F4 (08-JAN-08) Strain Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Naïve (unstimulated) cells from transgenic mice show no luciferase activity. When activated via addition of phorbol myristate acetate (PMA) plus ionomycin, cell populations from hemizygous mice exhibit high luciferase transcriptional activity in CD4+ T cells. Identically treated single-positive CD8+ T cells have lower transcriptional activity, while no luciferase is observed in B cell populations. Interleukin-2 transcriptional activators (anti-CD3 and ConA) also promote similar cell-specific patterns of reporter transcription. Luciferase expression is cyclosporine sensitive. Nuclear proteins isolated from hemizygous splenocytes show AP-1 specific binding to the transgenic TRE domain. This reporter transgene faithfully identifies AP-1 transcriptional activity both in vivo and in vitro. These mice may be useful in studies of immunology, including T cell receptor-mediated and co-stimulatory dependent T cell activation, signal transduction, apoptosis, and induced tumor models.Strain Development
A targeting vector was designed with the firefly luciferase gene (luc) driven by four copies of the human collagenase TPA-response element (TRE; AP-1 binding consensus sequence) in front of the rat prolactin (PRL) minimal promoter. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Founder line 31 was established and backcrossed to C57BL/6 mice for more than 15 generations prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(TRE/Prl-luc)31FlvRinc | ||
|---|---|---|---|
| Allele Name | transgene insertion 31, Richard Flavell and Mercedes Rincon | ||
| Common Name(s) | AP1-luc; TRE-LUC; | ||
| Mutation Made By | Dr. Mercedes Rincon, The University of Vermont | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | CD4+ T cells exhibit high luciferase transcriptional activity when activated via addition of phorbol myristate acetate (PMA) plus ionomycin. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Prl, prolactin, rat | ||
| Molecular Note | A transgenic construct was designed with the firefly luciferase gene (luc) driven by four copies of the human collagenase TPA-response element (TRE; AP-1 binding consensus sequence) in front of the rat prolactin (PRL) minimal promoter. [J:106464] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic positive mice are bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
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Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Transcriptional Regulation
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Research Tools
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Genetics Research (Tissue/Cell Markers: fos/luc)
References
Additional ReferencesPrice and Supply Information
| Strain Name: | B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J |
| Stock Number: | 006101 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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