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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.Cg-Tg(AP1/Prl-luc)31FlvRinc/J (Changed: 26-APR-06 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N16p Donating Investigator Dr. Mercedes Rincon, The University of Vermont Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Naïve (unstimulated) cells from transgenic mice show no luciferase activity. When activated via addition of phorbol myristate acetate (PMA) plus ionomycin, cell populations from hemizygous mice exhibit high luciferase transcriptional activity in CD4+ T cells. Identically treated single-positive CD8+ T cells have lower transcriptional activity, while no luciferase is observed in B cell populations. Interleukin-2 transcriptional activators (anti-CD3 and ConA) also promote similar cell-specific patterns of reporter transcription. Luciferase expression is cyclosporine sensitive. Nuclear proteins isolated from hemizygous splenocytes show AP-1 specific binding to the transgenic TRE domain. This reporter transgene faithfully identifies AP-1 transcriptional activity both in vivo and in vitro. These mice may be useful in studies of immunology, including T cell receptor-mediated and co-stimulatory dependent T cell activation, signal transduction, apoptosis, and induced tumor models.Development
A targeting vector was designed with the firefly luciferase gene (luc) driven by four copies of the human collagenase TPA-response element (TRE; AP-1 binding consensus sequence) in front of the rat prolactin (PRL) minimal promoter. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Founder line 31 was established and backcrossed to C57BL/6 mice for more than 15 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
010573 B6;SJL-Tg(Prl-tTA)6-5Jek/J
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J 005999 B6(SJL)-Tg(SBE/Tk-luc)7Twc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J 006851 B6.Cg-Tg(Per1-luc)025Jt/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J 009638 FVB-Tg(GFAP-luc,GAPDH-rluc)172.9Mes/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 002060 FVB/N-Tg(HIV-luc)326Morr/J 007800 FVB/N-Tg(Ins1-luc)VUPwrs/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Transcriptional Regulation
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Research Tools
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: transcriptional activation
Tissue/Cell Markers: fos/luc
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TRE/Prl-luc)31FlvRinc | ||
|---|---|---|---|
| Allele Name | transgene insertion 31, Richard Flavell and Mercedes Rincon | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | AP1-luc; TRE-LUC; | ||
| Mutation Made By | Dr. Mercedes Rincon, The University of Vermont | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | CD4+ T cells exhibit high luciferase transcriptional activity when activated via addition of phorbol myristate acetate (PMA) plus ionomycin. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Prl, prolactin, rat | ||
| Molecular Note | A transgenic construct was designed with the firefly luciferase gene (luc) driven by four copies of the human collagenase TPA-response element (TRE; AP-1 binding consensus sequence) in front of the rat prolactin (PRL) minimal promoter. [MGI Ref ID J:106464] | ||
| Gene Symbol and Name | Tg(TRE/Prl-luc)31FlvRinc, transgene insertion 31, Richard Flavell and Mercedes Rincon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | AP1-luc; TRE-LUC; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(luc), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Tg(TRE/Prl-luc)31FlvRinc relatedGerdes MJ; Myakishev M; Frost NA; Rishi V; Moitra J; Acharya A; Levy MR; Park SW; Glick A; Yuspa SH; Vinson C. 2006. Activator protein-1 activity regulates epithelial tumor cell identity. Cancer Res 66(15):7578-88. [PubMed: 16885357] [MGI Ref ID J:112098]
Leung-Theung-Long S; Mondor I; Guiraud M; Lamare C; Nageleekar V; Paulet PE; Rincon M; Guerder S. 2009. Impaired NFAT transcriptional activity in antigen-stimulated CD8 T cells linked to defective phosphorylation of NFAT transactivation domain. J Immunol 182(11):6807-14. [PubMed: 19454676] [MGI Ref ID J:148865]
Rincon M; Flavell RA. 1994. AP-1 transcriptional activity requires both T-cell receptor-mediated and co-stimulatory signals in primary T lymphocytes. EMBO J 13(18):4370-81. [PubMed: 7925281] [MGI Ref ID J:106464]
Rincon M; Flavell RA. 1996. Regulation of AP-1 and NFAT transcription factors during thymic selection of T cells. Mol Cell Biol 16(3):1074-84. [PubMed: 8622652] [MGI Ref ID J:106463]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic positive mice are bred.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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