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| These NFAT-luc transgenic mice may be useful in studies of immunology, cellular signaling, signal transduction, apoptosis, and research of transcription factors. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Major Histocompatibility Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N16+?p+F0p+N1
Generation DefinitionsDonating Investigator Dr. Mercedes Rincon, The University of Vermont Description
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that homozygous females in their colony are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for the NFAT inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These NFAT-luc transgenic mice may be useful be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation.Development
A targeting vector was designed with the firefly luciferase gene (luc) driven by ~ 150-bp of sequence from the interleukin-2 minimal promoter (nucleotides -326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Transgenic offspring (founder line 83) were crossed with B10.BR-H2k2 H2-T18a/SgSnJ (Stock No. 000465) mice for more than 15 generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000465 B10.BR-H2k2 H2-T18a/SgSnJ | ||
| 004804 B10.BR-H2k2 H2-T18a/SgSnJJrep | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 001148 B6.AK-H2k/FlaEgJ 001895 B6.AK-H2k/J 001383 LT.MA-Glo1b H2k/J
006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J
002252 B6.129P2-Il2tm1Hor/J 002229 C.129P2(B6)-Il2tm1Hor/J 002228 C3.129P2(B6)-Il2tm1Hor/J 000486 MRL/MpJ 002573 NOD.129P2(B6)-Il2tm1Hor/DvsJ 003852 NOD.B6-Il2C57BL/6/Lt 001289 NOD/ShiLt 001976 NOD/ShiLtJ 000686 SJL/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
H2k relatedCell Biology Research
Signal Transduction
Transcriptional Regulation
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Reproductive Biology Research
Fertility Defects
Research Tools
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: transcriptional activation
Tissue/Cell Markers
Tissue/Cell Markers: fos/luc
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | H2k | ||
|---|---|---|---|
| Allele Name | k variant | ||
| Allele Type | Not Applicable | ||
| Common Name(s) | H-2k; | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note | The k variant has been observed in the following strains: C3H, C3H/HeJ, C3H/An, C3H/Bi, C3H/DiSn, C3H/St, CBA, CBA/CaJ, CBA/N, AKR/J, CE/J, C57BR/cdJ, C58/J, MA/J, RF/J, ST/bJ, MRL/MpJ-Faslpr/J | ||
| Allele Symbol | Tg(Il2/NFAT-luc)83Rinc | ||
| Allele Name | transgene insertion 83, Mercedes Rincon | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | NFAT-luc; | ||
| Mutation Made By | Dr. Mercedes Rincon, The University of Vermont | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. NFAT is an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Il2, interleukin 2, mouse, laboratory | ||
| Molecular Note | A transgene construct was designed with the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 (IL-2) minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. [MGI Ref ID J:106466] | ||
| Gene Symbol and Name | Tg(Il2/NFAT-luc)83Rinc, transgene insertion 83, Mercedes Rincon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | NFAT-luc; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(luc), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
H2k relatedTg(Il2/NFAT-luc)83Rinc relatedBlazar BR; Carreno BM; Panoskaltsis-Mortari A; Carter L; Iwai Y; Yagita H; Nishimura H; Taylor PA. 2003. Blockade of programmed death-1 engagement accelerates graft-versus-host disease lethality by an IFN-gamma-dependent mechanism. J Immunol 171(3):1272-7. [PubMed: 12874215] [MGI Ref ID J:120213]
Cemerski S; Das J; Locasale J; Arnold P; Giurisato E; Markiewicz MA; Fremont D; Allen PM; Chakraborty AK; Shaw AS. 2007. The stimulatory potency of T cell antigens is influenced by the formation of the immunological synapse. Immunity 26(3):345-55. [PubMed: 17346997] [MGI Ref ID J:120073]
Chiorazzi N; Fox DA; Katz DH. 1977. Hapten-specific IgE antibody responses in mice. VII. Conversion of IgE non-responder strains to IgE responders by elimination of suppressor T cell activity. J Immunol 118(1):48-54. [PubMed: 299762] [MGI Ref ID J:5739]
Clarke SR; Barnden M; Kurts C; Carbone FR; Miller JF; Heath WR. 2000. Characterization of the ovalbumin-specific TCR transgenic line OT-I: MHC elements for positive and negative selection. Immunol Cell Biol 78(2):110-7. [PubMed: 10762410] [MGI Ref ID J:133645]
Fischer Lindahl K. 1997. On naming H2 haplotypes: functional significance of MHC class Ib alleles. Immunogenetics 46(1):53-62. [PubMed: 9148789] [MGI Ref ID J:41130]
Gustavsson S; Wernersson S; Heyman B. 2000. Restoration of the antibody response to IgE/antigen complexes in CD23-deficient mice by CD23+ spleen or bone marrow cells. J Immunol 164(8):3990-5. [PubMed: 10754289] [MGI Ref ID J:112143]
Hegde S; Niederkorn JY. 2000. The role of cytotoxic T lymphocytes in corneal allograft rejection. Invest Ophthalmol Vis Sci 41(11):3341-7. [PubMed: 11006223] [MGI Ref ID J:115389]
Jenkinson EJ; Van Ewijk W; Owen JJ. 1981. Major histocompatibility complex antigen expression on the epithelium of the developing thymus in normal and nude mice. J Exp Med 153(2):280-92. [PubMed: 6940949] [MGI Ref ID J:109960]
Kaye J; Vasquez NJ; Hedrick SM. 1992. Involvement of the same region of the T cell antigen receptor in thymic selection and foreign peptide recognition. J Immunol 148(11):3342-53. [PubMed: 1316916] [MGI Ref ID J:124701]
Klein J; Figueroa F; David CS. 1983. H-2 haplotypes, genes and antigens: second listing. II. The H-2 complex. Immunogenetics 17(6):553-96. [PubMed: 6407984] [MGI Ref ID J:7097]
Lakkis FG; Arakelov A; Konieczny BT; Inoue Y. 2000. Immunologic 'ignorance' of vascularized organ transplants in the absence of secondary lymphoid tissue. Nat Med 6(6):686-8. [PubMed: 10835686] [MGI Ref ID J:118716]
Le PH; Leiter EH; Leyendecker JR. 1985. Genetic control of susceptibility to streptozotocin diabetes in inbred mice: effect of testosterone and H-2 haplotype. Endocrinology 116(6):2450-5. [PubMed: 3888611] [MGI Ref ID J:109951]
Liu P; Aitken K; Kong YY; Opavsky MA; Martino T; Dawood F; Wen WH; Kozieradzki I; Bachmaier K; Straus D; Mak TW; Penninger JM. 2000. The tyrosine kinase p56lck is essential in coxsackievirus B3-mediated heart disease. Nat Med 6(4):429-34. [PubMed: 10742150] [MGI Ref ID J:119597]
Lucas JA; Atherly LO; Berg LJ. 2002. The absence of Itk inhibits positive selection without changing lineage commitment. J Immunol 168(12):6142-51. [PubMed: 12055226] [MGI Ref ID J:123795]
Mendiratta SK; Kovalik JP; Hong S; Singh N; Martin WD; Van Kaer L. 1999. Peptide dependency of alloreactive CD4+ T cell responses. Int Immunol 11(3):351-60. [PubMed: 10221647] [MGI Ref ID J:110542]
Merino R; Shibata T; De Kossodo S; Izui S. 1989. Differential effect of the autoimmune Yaa and lpr genes on the acceleration of lupus-like syndrome in MRL/MpJ mice. Eur J Immunol 19(11):2131-7. [PubMed: 2599002] [MGI Ref ID J:108759]
Miyashita N; Migita S; Moriwaki K. 1987. Effects of H-2 complex and non-H-2 background on urethane-induced chromosomal aberrations in mice. Mutat Res 176(1):59-67. [PubMed: 3099189] [MGI Ref ID J:109945]
Murphy DB. 1986. Overview: the murine MHC. In: Handbook of Experimental Immunology. Vol. 3, Genetic and Molecular Immunology. Blackwell Scientific Publ., Oxford. [MGI Ref ID J:30731]
Panoskaltsis-Mortari A; Hermanson JR; Taras E; Wangensteen OD; Charo IF; Rollins BJ; Blazar BR. 2004. Post-BMT lung injury occurs independently of the expression of CCL2 or its receptor, CCR2, on host cells. Am J Physiol Lung Cell Mol Physiol 286(2):L284-92. [PubMed: 14527928] [MGI Ref ID J:101226]
Podolin PL; Pressey A; DeLarato NH; Fischer PA; Peterson LB; Wicker LS. 1993. I-E+ nonobese diabetic mice develop insulitis and diabetes. J Exp Med 178(3):793-803. [PubMed: 8350054] [MGI Ref ID J:14178]
Qiu H; Wang S; Yang J; Fan Y; Joyee AG; Han X; Jiao L; Yang X. 2005. Resistance to chlamydial lung infection is dependent on major histocompatibility complex as well as non-major histocompatibility complex determinants. Immunology 116(4):499-506. [PubMed: 16313363] [MGI Ref ID J:106140]
Sharma RB; Alegria JD; Talor MV; Rose NR; Caturegli P; Burek CL. 2005. Iodine and IFN-gamma synergistically enhance intercellular adhesion molecule 1 expression on NOD.H2h4 mouse thyrocytes. J Immunol 174(12):7740-5. [PubMed: 15944276] [MGI Ref ID J:100880]
Smyth LA; Ratnasothy K; Moreau A; Alcock S; Sagoo P; Meader L; Tanriver Y; Buckland M; Lechler R; Lombardi G. 2013. Tolerogenic Donor-Derived Dendritic Cells Risk Sensitization In Vivo owing to Processing and Presentation by Recipient APCs. J Immunol 190(9):4848-60. [PubMed: 23536635] [MGI Ref ID J:195514]
Sutton VR; Waterhouse NJ; Browne KA; Sedelies K; Ciccone A; Anthony D; Koskinen A; Mullbacher A; Trapani JA. 2007. Residual active granzyme B in cathepsin C-null lymphocytes is sufficient for perforin-dependent target cell apoptosis. J Cell Biol 176(4):425-33. [PubMed: 17283185] [MGI Ref ID J:119725]
Venkatesan P; Finch RG; Wakelin D. 1993. MHC haplotype influences primary Giardia muris infections in H-2 congenic strains of mice. Int J Parasitol 23(5):661-4. [PubMed: 8225770] [MGI Ref ID J:21687]
Willse A; Kwak J; Yamazaki K; Preti G; Wahl JH; Beauchamp GK. 2006. Individual odortypes: interaction of MHC and background genes. Immunogenetics 58(12):967-82. [PubMed: 17089117] [MGI Ref ID J:116154]
Zhang ZX; Yang L; Young KJ; DuTemple B; Zhang L. 2000. Identification of a previously unknown antigen-specific regulatory T cell and its mechanism of suppression. Nat Med 6(7):782-9. [PubMed: 10888927] [MGI Ref ID J:118729]
Zhou P; Szot GL; Guo Z; Kim O; He G; Wang J; Grusby MJ; Newell KA; Thistlethwaite JR; Bluestone JA; Alegre ML. 2000. Role of STAT4 and STAT6 signaling in allograft rejection and CTLA4-Ig-mediated tolerance. J Immunol 165(10):5580-7. [PubMed: 11067913] [MGI Ref ID J:119580]
Leung-Theung-Long S; Mondor I; Guiraud M; Lamare C; Nageleekar V; Paulet PE; Rincon M; Guerder S. 2009. Impaired NFAT transcriptional activity in antigen-stimulated CD8 T cells linked to defective phosphorylation of NFAT transactivation domain. J Immunol 182(11):6807-14. [PubMed: 19454676] [MGI Ref ID J:148865]
Rincon M; Flavell RA. 1996. Regulation of AP-1 and NFAT transcription factors during thymic selection of T cells. Mol Cell Biol 16(3):1074-84. [PubMed: 8622652] [MGI Ref ID J:106463]
Rincon M; Flavell RA. 1997. Transcription mediated by NFAT is highly inducible in effector CD4+ T helper 2 (Th2) cells but not in Th1 cells. Mol Cell Biol 17(3):1522-34. [PubMed: 9032280] [MGI Ref ID J:106462]
Verweij CL; Guidos C; Crabtree GR. 1990. Cell type specificity and activation requirements for NFAT-1 (nuclear factor of activated T-cells) transcriptional activity determined by a new method using transgenic mice to assay transcriptional activity of an individual nuclear factor. J Biol Chem 265(26):15788-95. [PubMed: 2394747] [MGI Ref ID J:106466]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. The donating investigator reports that homozygous females in their colony are subfertile.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000465 B10.BR-H2k2 H2-T18a/SgSnJ | ||
| 004804 B10.BR-H2k2 H2-T18a/SgSnJJrep | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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