Strain Name: |
B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J |
|---|---|
Stock Number: |
006102 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | H2; H2k; Il2; Nfatc2; luc; Tg(Il2/NFAT-luc)83Rinc; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Major Histocompatibility Congenic Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Dr. Mercedes Rincon, The University of Vermont Generation N16+?p+F0 (18-DEC-06) Strain Description
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that homozygous females in their colony are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for the NFAT inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These NFAT-luc transgenic mice may be useful be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation.Strain Development
A targeting vector was designed with the firefly luciferase gene (luc) driven by ~ 150-bp of sequence from the interleukin-2 minimal promoter (nucleotides -326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Transgenic offspring (founder line 83) were crossed with "B10.BR/SgSnJ (H2-K)" (see Stock No. 000465) mice for more than 15 generations before arriving at The Jackson Laboratory.
| Allele Symbol | H2k | ||
|---|---|---|---|
| Allele Name | k variant | ||
| Common Name(s) | H-2k; | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note | The k variant has been observed in the following strains: C3H, C3H/HeJ, C3H/An, C3H/Bi, C3H/DiSn, C3H/St, CBA, CBA/CaJ, CBA/N, AKR/J, CE/J, C57BR/cdJ, C58/J, MA/J, RF/J, ST/bJ, MRL/MpJ-Faslpr/J | ||
| Allele Symbol | Tg(Il2/NFAT-luc)83Rinc | ||
| Allele Name | transgene insertion 83, Mercedes Rincon | ||
| Common Name(s) | NFAT-luc; | ||
| Mutation Made By | Dr. Mercedes Rincon, The University of Vermont | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. NFAT is an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Il2, interleukin 2, mouse, laboratory | ||
| Promoter | Nfatc2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, mouse, laboratory | ||
| Molecular Note | A transgene construct was designed with the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 (IL-2) minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. [J:106466] | ||
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000465 B10.BR-H2k H2-T18a/SgSnJ | ||
| 004804 B10.BR-H2k H2-T18a/SgSnJJrep | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Luc
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. The donating investigator reports that homozygous females in their colony are subfertile. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
Strains carrying H2k allele
000465 B10.BR-H2k H2-T18a/SgSnJ 004804 B10.BR-H2k H2-T18a/SgSnJJrep 006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 001148 B6.AK-H2k/FlaEgJ 001895 B6.AK-H2k/J 001951 C.C3-H2k/LilMcdJ 001383 LT.MA-Glo1b H2k/J View Strains carrying H2k (7 strains)
Strains carrying Tg(Il2/NFAT-luc)83Rinc allele
006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J View Strains carrying Tg(Il2/NFAT-luc)83Rinc (1 strain)
Strains carrying other alleles of H2
View Strains carrying other alleles of H2 (51 strains)
Strains carrying other alleles of Il2
002252 B6.129P2-Il2tm1Hor/J 002229 C.129P2(B6)-Il2tm1Hor/J 002228 C3.129P2(B6)-Il2tm1Hor/J 002573 NOD.129P2(B6)-Il2tm1Hor/DvsJ View Strains carrying other alleles of Il2 (4 strains)
Strains carrying other alleles of luc
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 005999 B6(SJL)-Tg(SBE/Tk-luc)7Twc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 002060 FVB/N-Tg(HIV-luc)326Morr/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J View Strains carrying other alleles of luc (9 strains)
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Room Number AX11
H2k relatedCell Biology Research
Signal Transduction
Transcriptional Regulation
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Reproductive Biology Research
Fertility Defects
Research Tools
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Genetics Research (Tissue/Cell Markers: fos/luc)
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
| Strain Name: | B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J |
| Stock Number: | 006102 |
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(Il2/NFAT-luc)83Rinc | |||
| Pair | $288.65 | Hemizygous for Tg(Il2/NFAT-luc)83Rinc x Noncarrier | |||
| Pair | $288.65 | Noncarrier x Hemizygous for Tg(Il2/NFAT-luc)83Rinc | |||
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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