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Strain Name:

B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J

Stock Number:

006102

Availability:

Repository- Live


Price and Supply Information

General Terms and Conditions

Genes & Alleles   H2;   H2k;   Il2;   Nfatc2;   luc;   Tg(Il2/NFAT-luc)83Rinc;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Major Histocompatibility Congenic
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating InvestigatorDr. Mercedes Rincon,   The University of Vermont
GenerationN16+?p+F0 (18-DEC-06)

Strain Description
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that homozygous females in their colony are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for the NFAT inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These NFAT-luc transgenic mice may be useful be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation.

Strain Development
A targeting vector was designed with the firefly luciferase gene (luc) driven by ~ 150-bp of sequence from the interleukin-2 minimal promoter (nucleotides -326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. This construct was microinjected into (C57BL6 x SJL)F2 embryos. Transgenic offspring (founder line 83) were crossed with "B10.BR/SgSnJ (H2-K)" (see Stock No. 000465) mice for more than 15 generations before arriving at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol H2k
Allele Name k variant
Common Name(s) H-2k;
Gene Symbol and Name H2, histocompatibility-2, MHC
Chromosome 17
Gene Common Name(s) H-2; MHC-II;
General Note The k variant has been observed in the following strains: C3H, C3H/HeJ, C3H/An, C3H/Bi, C3H/DiSn, C3H/St, CBA, CBA/CaJ, CBA/N, AKR/J, CE/J, C57BR/cdJ, C58/J, MA/J, RF/J, ST/bJ, MRL/MpJ-Faslpr/J
 
Allele Symbol Tg(Il2/NFAT-luc)83Rinc
Allele Name transgene insertion 83, Mercedes Rincon
Common Name(s) NFAT-luc;
Mutation Made ByDr. Mercedes Rincon,   The University of Vermont
Strain of Origin(C57BL/6 x SJL)F2
Site of ExpressionLuciferase activity in these transgenic mice identifies NFAT-mediated transcription. NFAT is an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression.
Expressed Gene luc, luciferase, firefly
Promoter Il2, interleukin 2, mouse, laboratory
Promoter Nfatc2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, mouse, laboratory
Molecular Note A transgene construct was designed with the firefly luciferase gene (luc) driven by a 200-bp fragment of the interleukin-2 (IL-2) minimal promoter (-326 to -294 and -72 to +47) with three NFAT-binding sites inserted between these fragments. [J:106466]

Control Information

  Control
   Noncarrier
   000465 B10.BR-H2k H2-T18a/SgSnJ
   004804 B10.BR-H2k H2-T18a/SgSnJJrep
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Luc

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as hemizygotes. The donating investigator reports that homozygous females in their colony are subfertile.
Diet Information LabDiet® 5K52/5K67

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004858   B6;129S1-Tshrtm1Rmar/J
007843   B6;129S4-Efnb2tm2Sor/J
008214   B6;129S4-Pou5f1tm2Jae/J
006614   B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J
006617   B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
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005620   B6;D2-Tg(S100B-EYFP)1Wjt/J
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006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
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005700   C.129P2-Cxcr6tm1Litt/J
006769   C.Cg-Foxp3tm2Tch/J
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Mass/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
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003718   FVB-Tg(GadGFP)45704Swn/J
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003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
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005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
006741   STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)BMom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
006570   STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
007879   STOCK Stx1atm2Sud/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
006784   STOCK Tg(Ins1-ECFP)24Hara/J
003658   STOCK Tg(TIE2GFP)287Sato/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
003274   STOCK Tg(tetNZL)2Bjd/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Fluorescent Protein Strains     (138 strains)

Strains carrying   H2k allele
000465   B10.BR-H2k H2-T18a/SgSnJ
004804   B10.BR-H2k H2-T18a/SgSnJJrep
006100   B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J
001148   B6.AK-H2k/FlaEgJ
001895   B6.AK-H2k/J
001951   C.C3-H2k/LilMcdJ
001383   LT.MA-Glo1b H2k/J
View Strains carrying   H2k     (7 strains)

Strains carrying   Tg(Il2/NFAT-luc)83Rinc allele
006098   B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J
View Strains carrying   Tg(Il2/NFAT-luc)83Rinc     (1 strain)

Strains carrying other alleles of H2
000471   A.SW-H2s H2-T18b/SnJ
002089   AK.B6-H2b Fv1b/J
002090   AK.B6-H2b/J
001094   AK.L-H2b/1CyTyJ
003851   ALR.NOD-(D17Mit30-D17Mit123)/Lt
000469   B10.A-H2a H2-T18a/SgSnJ
000468   B10.A-H2h2/(2R)SgSnJ
001150   B10.A-H2h4/(4R)SgDvEgJ
000467   B10.A-H2i5 H2-T18a/(5R)SgSnJ
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005534   B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002024   B10.D1-H2q/SgJ
000462   B10.D2-H2d/n2SnJ
001153   B10.D2-H2i7/(107R)EgJ
000460   B10.D2-Hc0 H2d H2-T18c/o2SnJ
000461   B10.D2-Hc0 H2d H2-T18c/oSnJ
000463   B10.D2-Hc1 H2d H2-T18c/nSnJ
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
001953   B10.S-H2s/SgMcdJ
002995   B6 x C.B10-H2b/LiMcdJ-Fbn2fp-2J/J
005717   B6(NOD) H2g7-Sostdc1shk/J
003584   B6.129S2-H2dlAb1-Ea/J
000360   B6.C-H2d Mdmg1BALB/cBy/aByJ
000359   B6.C-H2d/bByJ
005715   B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ
003068   B6.NOD-(Csf2-D11Mit42) (D17Mit21-D17Mit10)/J
003300   B6.NOD-(D17Mit21-D17Mit10)/LtJ
003069   B6.NOD-(D1Mit3-Bcl2) (D17Mit21-D17Mit10)/LtJ
003071   B6.NOD-(D1Mit5.1-D1Mit15) (D17Mit21-D17Mit10)/J
003067   B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J
003066   B6.NOD-(D6Mit54-D6Mit14) (D17Mit21-D17Mit10)/J
000944   B6.SJL-H2b C3c/2CyJ
000966   B6.SJL-H2s C3c/1CyJ
000945   B6.SW/1CyJ
003374   B6;129S2-H2dlAb1-Ea/J
003240   B6;B10.A-H2a-Tg(H2KmPCC)2939Stoe/J
002844   BALB.5R-H2i5/LilJ
001041   BKS.B6-H2b/J
001892   BRVR.B10-H2b/J
001893   BRVR.D2-H2d/J
001952   C.B10-H2b/LilMcdJ
000438   C3.SW-H2b/SnJ
000437   D1.C-H2d H2-T18c/SnJ
000435   D1.LP-H2b H2-T18b?/SnJ
002591   NOD.B10Sn-H2b/J
004447   NOD.Cg-H2h4/DilTacUmmJ
002032   NOD.SW-H2q/J
001627   NON.NOD-H2g7/LtJ
003153   WLC.C-H2d.GR-Mtv2/MorJ
003154   WLC.C-H2d/MorJ
View Strains carrying other alleles of H2     (51 strains)

Strains carrying other alleles of Il2
002252   B6.129P2-Il2tm1Hor/J
002229   C.129P2(B6)-Il2tm1Hor/J
002228   C3.129P2(B6)-Il2tm1Hor/J
002573   NOD.129P2(B6)-Il2tm1Hor/DvsJ
View Strains carrying other alleles of Il2     (4 strains)

View Strains carrying other alleles of luc     (9 strains)

Additional Web Information

Congenic Nomenclature
Fluorescent Proteins/lacZ Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction
Transcriptional Regulation

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Reproductive Biology Research
Fertility Defects

Research Tools
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Genetics Research (Tissue/Cell Markers: fos/luc)

H2k related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

References

Additional References

Price and Supply Information

Strain Name: B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
Stock Number: 006102

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)Genotype(s) Provided
Individual Mouse Price $236.40Hemizygous for Tg(Il2/NFAT-luc)83Rinc
Pair $288.65Hemizygous for Tg(Il2/NFAT-luc)83Rinc x Noncarrier
Pair $288.65Noncarrier x Hemizygous for Tg(Il2/NFAT-luc)83Rinc

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423.
Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.
View Control Pricing Information for JAX® Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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