Strain Name:

B6.129S4-Mbl1tm1Kata Mbl2tm1Kata/J

Stock Number:

006122

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Availability:

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Use Restrictions Apply, see Terms of Use
Common Names: MBL-null;    
Mice homozygous for both mannose-binding lectin knock-out mutations have a non-functional lectin-dependent complement pathway. These mice may be useful in studies of host defense, including inflammation and infection, chemotherapy-induced neutropenia, apoptosis, and gastrointestinal ischemia/reperfusion models of the complement pathway.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-AUG-06
Specieslaboratory mouse
GenerationN7+N1F3 (10-DEC-13)
Generation Definitions
 
Donating Investigator Kazue Takahashi,   Massachusetts General Hospital

Description
Mice homozygous for both mannose-binding lectin (MBL)-A and MBL-C targeted mutations (termed MBL-null) are viable, fertile, and normal in size with no obvious developmental defects. Histological examination of multiple organs from 6-10 week old mice shows no abnormalities. MBL-null mice have no endogenous gene expression in liver (the principal site of MBL synthesis) and no protein detectable in serum. While the classical complement pathway is unaffected in MBL-null mice, the lectin-dependent complement pathway is non-functional. MBL-null mice have increased mortality following intravenous injection of S. aureus associated with abnormal serum levels of TNFalpha and IL-6 (decreased at 2h, elevated at 24h post injection). Cyclophosphamide-induced febrile neutropenic MBL-null mice inoculated with S. aureus have greatly increased susceptibility to abscess formation in kidney, liver, and lung (but not spleen). These same treated mice also have persistent bacteremia despite a recovery of circulating neutrophils and reduction of both circulating monocytes and resident peritoneal macrophages. Resident peritoneal macrophages harvested following intraperitoneal injection of S. aureus have impaired bacterial phagocytosis. MBL-null mice have an expansion of peritoneal B1 cells but a decreased splenic B cell p opulation. Double mutant mice have defective apoptotic cell clearance with no autoimmune phenotype out to 18 months of age. MBL-null mice have increased susceptibility to postburn infection with P. aeruginosa and defective hepatic clearance of virus following herpes simplex virus-2 injection. Double mutant mice insulted with gastrointestinal ischemia/reperfusion (I/R) are protected from intestinal, but not secondary lung, injury and C3 accumulation. Mutant mice are also protected from injury and C3 accumulation following bilateral renal I/R and myocardial I/R. These mice may be useful in studies of host defense, including inflammation and infection, chemotherapy-induced neutropenia, apoptosis, and I/R models of the complement pathway.

Development
A targeting vector was designed to insert a neomycin resistance gene into exon 5 of the MBL-A (Mbl1) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into C57BL/6J blastocysts and chimeric offspring were bred with C57BL/6J mice for multiple generations and then made homozygous. Independently, a construct was designed to replace exon 6 of the MBL-C (Mbl2) gene with a neomycin resistance gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into C57BL/6J blastocysts. Chimeric offspring were bred to make homozygous mutant mice. To generate mice homozygous for both mutations (MBL-null), MBL-A mutant homozygotes were bred with homozygous MBL-C mutant mice. The donating investigator reported that double mutant mice were backcrossed to C57BL/6J for 7 generations before being made homozygous (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Mannose-Binding Protein Deficiency   (MBL2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mbl1tm1Kata/Mbl1tm1Kata Mbl2tm1Kata/Mbl2tm1Kata

        involves: 129 * C57BL/6
  • immune system phenotype
  • abnormal B cell number   (MGI Ref ID J:97686)
    • decreased germinal center B cell number
      • more than 60% decrease in germinal center B cell numbers   (MGI Ref ID J:97686)
    • increased B-1 B cell number
      • increased number of peritoneal B1 cells, however no lymphoproliferation occurred   (MGI Ref ID J:97686)
  • abnormal circulating interleukin-6 level
    • serum IL-6 levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 8-fold at 24 hours after inoculation compared to wildtype   (MGI Ref ID J:98124)
  • abnormal circulating tumor necrosis factor level
    • serum TNF-alpha levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 15-fold at 24 hours after inoculation compared to wildtype   (MGI Ref ID J:98124)
  • decreased macrophage cell number
    • double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in resident peritoneal macrophages   (MGI Ref ID J:98124)
  • decreased monocyte cell number
    • double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in circulating monocytes   (MGI Ref ID J:98124)
  • impaired macrophage phagocytosis
    • demonstrated defective apoptotic cell clearance (16% of macrophages phagocytosed compared to more than 25% in wildtype), however did not develop spontaneous autoimmunity   (MGI Ref ID J:97686)
    • 38% decrease in percentage of phagocytic cells   (MGI Ref ID J:97686)
    • showed a 40% reduction in bacterial phagocytosis by resident peritoneal macrophages   (MGI Ref ID J:98124)
  • increased susceptibility to bacterial infection
    • 100% of double mutants died 48 hours after S. aureus infection compared to 45% of wildtype   (MGI Ref ID J:98124)
    • statistically significant higher bacterial loads were found in the kidney, spleen, liver and blood compared with wildtype at 24 hours after S. aureus inoculation   (MGI Ref ID J:98124)
    • double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection exhibited increased susceptibility to bacterial infection with 21/29 developing abscesses in various organs compared to 3/15 of wildtype   (MGI Ref ID J:98124)
  • hematopoietic system phenotype
  • abnormal B cell number   (MGI Ref ID J:97686)
    • decreased germinal center B cell number
      • more than 60% decrease in germinal center B cell numbers   (MGI Ref ID J:97686)
    • increased B-1 B cell number
      • increased number of peritoneal B1 cells, however no lymphoproliferation occurred   (MGI Ref ID J:97686)
  • decreased macrophage cell number
    • double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in resident peritoneal macrophages   (MGI Ref ID J:98124)
  • decreased monocyte cell number
    • double homozygous mutants rendered neutropenic by cyclophosphamide (CY) injection had an 80% decrease in circulating monocytes   (MGI Ref ID J:98124)
  • impaired macrophage phagocytosis
    • demonstrated defective apoptotic cell clearance (16% of macrophages phagocytosed compared to more than 25% in wildtype), however did not develop spontaneous autoimmunity   (MGI Ref ID J:97686)
    • 38% decrease in percentage of phagocytic cells   (MGI Ref ID J:97686)
    • showed a 40% reduction in bacterial phagocytosis by resident peritoneal macrophages   (MGI Ref ID J:98124)
  • homeostasis/metabolism phenotype
  • abnormal circulating interleukin-6 level
    • serum IL-6 levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 8-fold at 24 hours after inoculation compared to wildtype   (MGI Ref ID J:98124)
  • abnormal circulating tumor necrosis factor level
    • serum TNF-alpha levels were reduced at 2 hours after S. aureus inoculation, however levels were increased 15-fold at 24 hours after inoculation compared to wildtype   (MGI Ref ID J:98124)

Mbl1tm1Kata/Mbl1tm1Kata Mbl2tm1Kata/Mbl2tm1Kata

        involves: 129 * C57BL/6J
  • immune system phenotype
  • abnormal complement pathway
    • after 45 min ischemia followed by 2 h reperfusion, complement activation as assessed by plasma C3a (desArg) level is significantly greater in wild-type mice than in double homozygous mutants; by 24 h, both groups have C3a levels < 30 ng/ml   (MGI Ref ID J:106351)
  • abnormal response to infection
    • doubly homozygous mutant mice infected intraperitoneally with herpes simplex virus type II (HSV-2) begin to clear the infection more slowly from the liver than do wild-type mice; however, complete clearance occurs in both by 6 days post-infection   (MGI Ref ID J:106352)
    • the proportion of HSV-2 neutralized in vitro after 9 minutes' exposure to serum from double homozygotes is significantly lower than after incubation with serum from wild-type mice; however, both have neutralized 90% of virus after > 21 minutes   (MGI Ref ID J:106352)
    • increased susceptibility to bacterial infection
      • all doubly homozygous mutant mice, but only 30% of wild-type mice, die within 42 h after burning of 5% of total body surface area (TBSA) followed by subcutaneous (s.c.) inoculation with Pseudomonas aeruginosa, whereas neither mutant nor wild-type mice appear ill following either treatment alone   (MGI Ref ID J:106353)
      • P. aeruginosa titers in plasma and homogenates of blood, liver and kidney harvested 20 h after the postburn inoculation are significantly higher in double homozygous than in wild-type mice; however, titers in lung and in skin do not differ significantly   (MGI Ref ID J:106353)
  • renal/urinary system phenotype
  • decreased susceptibility to kidney reperfusion injury
    • doubly homozygous mutant mice exhibit significantly less elevation of blood urea nitrogen (BUN) levels than do wild-type mice following induction of bilateral kidney eschemia (45 min) and reperfusion (24 h)   (MGI Ref ID J:106351)
    • after 45 min ischemia followed by 2 or 24 h reperfusion, histological examination of wild-type kidneys reveals severe tubular damage characterized by dilation, necrosis and presence of proteinaceous casts; kidneys of double homozygous mice exhibit no such damage   (MGI Ref ID J:106351)
  • homeostasis/metabolism phenotype
  • decreased susceptibility to kidney reperfusion injury
    • doubly homozygous mutant mice exhibit significantly less elevation of blood urea nitrogen (BUN) levels than do wild-type mice following induction of bilateral kidney eschemia (45 min) and reperfusion (24 h)   (MGI Ref ID J:106351)
    • after 45 min ischemia followed by 2 or 24 h reperfusion, histological examination of wild-type kidneys reveals severe tubular damage characterized by dilation, necrosis and presence of proteinaceous casts; kidneys of double homozygous mice exhibit no such damage   (MGI Ref ID J:106351)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Other

Cardiovascular Research
Ischemia studies

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      specific complement deficiency
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      genes regulating susceptibility to infectious disease and endotoxin
Immunodeficiency
      defects in humoral immune responses
      multiple immune defects
      specific complement deficiency
Immunodeficiency Associated with Other Defects

Research Tools
Apoptosis Research
Cancer Research
Immunology, Inflammation and Autoimmunity Research
      genes regulating susceptibility to infectious disease and endotoxin
      specific complement deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mbl1tm1Kata
Allele Name targeted mutation 1, Kazue Takahashi
Allele Type Targeted (Null/Knockout)
Common Name(s) MBL-A-;
Mutation Made By Kazue Takahashi,   Massachusetts General Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Mbl1, mannose-binding lectin (protein A) 1
Chromosome 14
Gene Common Name(s) MBL-A; MBP-A; Mbpa; Mlb1;
Molecular Note Exon 5 was disrupted by the insertion of a neomycin selection cassette into sequence encoding the carbohydrate recognition domain. Transcript was undetected by RT-PCR analysis of liver RNA obtained from homozygous mutant mice. RT-PCR analysis indicated that transcription of the paralogous Mbl2 gene was unaffected. [MGI Ref ID J:90437]
 
Allele Symbol Mbl2tm1Kata
Allele Name targeted mutation 1, Kazue Takahashi
Allele Type Targeted (Null/Knockout)
Common Name(s) MBL-C KO;
Mutation Made By Kazue Takahashi,   Massachusetts General Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Mbl2, mannose-binding lectin (protein C) 2
Chromosome 19
Gene Common Name(s) Ab2-001; Ab2-011; COLEC1; HSMBPC; MBL; MBL-C; MBL2D; MBP; MBP-C; MBP1; MBPD;
Molecular Note The gene was disrupted by the insertion of a neomycin resistance gene into exon 6. RT-PCR analysis demonstrated the lack of transcript in mutant liver. [MGI Ref ID J:98124]

Genotyping

Genotyping Information

Genotyping Protocols

Mbl1tm1Kata, Separated PCR
Mbl2tm1Kata, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shi L; Takahashi K; Dundee J; Shahroor-Karni S; Thiel S; Jensenius JC; Gad F; Hamblin MR; Sastry KN; Ezekowitz RA. 2004. Mannose-binding lectin-deficient mice are susceptible to infection with Staphylococcus aureus. J Exp Med 199(10):1379-90. [PubMed: 15148336]  [MGI Ref ID J:98124]

Additional References

Mbl1tm1Kata related

Ali YM; Lynch NJ; Haleem KS; Fujita T; Endo Y; Hansen S; Holmskov U; Takahashi K; Stahl GL; Dudler T; Girija UV; Wallis R; Kadioglu A; Stover CM; Andrew PW; Schwaeble WJ. 2012. The lectin pathway of complement activation is a critical component of the innate immune response to pneumococcal infection. PLoS Pathog 8(7):e1002793. [PubMed: 22792067]  [MGI Ref ID J:195379]

Banda NK; Levitt B; Wood AK; Takahashi K; Stahl GL; Holers VM; Arend WP. 2009. Complement activation pathways in murine immune complex-induced arthritis and in C3a and C5a generation in vitro. Clin Exp Immunol 159(1):100-8. [PubMed: 19843088]  [MGI Ref ID J:155358]

Banda NK; Takahashi K; Wood AK; Holers VM; Arend WP. 2007. Pathogenic complement activation in collagen antibody-induced arthritis in mice requires amplification by the alternative pathway. J Immunol 179(6):4101-9. [PubMed: 17785849]  [MGI Ref ID J:152026]

Banda NK; Takahashi M; Takahashi K; Stahl GL; Hyatt S; Glogowska M; Wiles TA; Endo Y; Fujita T; Michael Holers V; Arend WP. 2011. Mechanisms of mannose-binding lectin-associated serine proteases-1/3 activation of the alternative pathway of complement. Mol Immunol 49(1-2):281-9. [PubMed: 21943708]  [MGI Ref ID J:177350]

Bidula S; Kenawy H; Ali YM; Sexton D; Schwaeble WJ; Schelenz S. 2013. Role of Ficolin-A and Lectin Complement Pathway in the Innate Defense against Pathogenic Aspergillus Species. Infect Immun 81(5):1730-40. [PubMed: 23478320]  [MGI Ref ID J:194903]

Busche MN; Pavlov V; Takahashi K; Stahl GL. 2009. Myocardial ischemia and reperfusion injury is dependent on both IgM and mannose-binding lectin. Am J Physiol Heart Circ Physiol 297(5):H1853-9. [PubMed: 19749170]  [MGI Ref ID J:154309]

Busche MN; Walsh MC; McMullen ME; Guikema BJ; Stahl GL. 2008. Mannose-binding lectin plays a critical role in myocardial ischaemia and reperfusion injury in a mouse model of diabetes. Diabetologia 51(8):1544-51. [PubMed: 18493734]  [MGI Ref ID J:137894]

Cervera A; Planas AM; Justicia C; Urra X; Jensenius JC; Torres F; Lozano F; Chamorro A. 2010. Genetically-defined deficiency of mannose-binding lectin is associated with protection after experimental stroke in mice and outcome in human stroke. PLoS One 5(2):e8433. [PubMed: 20140243]  [MGI Ref ID J:158006]

Chan RK; Ibrahim SI; Takahashi K; Kwon E; McCormack M; Ezekowitz A; Carroll MC; Moore FD Jr; Austen WG Jr. 2006. The differing roles of the classical and mannose-binding lectin complement pathways in the events following skeletal muscle ischemia-reperfusion. J Immunol 177(11):8080-5. [PubMed: 17114482]  [MGI Ref ID J:140685]

Gadjeva M; Paludan SR; Thiel S; Slavov V; Ruseva M; Eriksson K; Lowhagen GB; Shi L; Takahashi K; Ezekowitz A; Jensenius JC. 2004. Mannan-binding lectin modulates the response to HSV-2 infection. Clin Exp Immunol 138(2):304-11. [PubMed: 15498041]  [MGI Ref ID J:106352]

Gonzalez SF; Lukacs-Kornek V; Kuligowski MP; Pitcher LA; Degn SE; Kim YA; Cloninger MJ; Martinez-Pomares L; Gordon S; Turley SJ; Carroll MC. 2010. Capture of influenza by medullary dendritic cells via SIGN-R1 is essential for humoral immunity in draining lymph nodes. Nat Immunol 11(5):427-34. [PubMed: 20305659]  [MGI Ref ID J:158970]

Gunn BM; Morrison TE; Whitmore AC; Blevins LK; Hueston L; Fraser RJ; Herrero LJ; Ramirez R; Smith PN; Mahalingam S; Heise MT. 2012. Mannose binding lectin is required for alphavirus-induced arthritis/myositis. PLoS Pathog 8(3):e1002586. [PubMed: 22457620]  [MGI Ref ID J:195398]

Hart ML; Ceonzo KA; Shaffer LA; Takahashi K; Rother RP; Reenstra WR; Buras JA; Stahl GL. 2005. Gastrointestinal ischemia-reperfusion injury is lectin complement pathway dependent without involving C1q. J Immunol 174(10):6373-80. [PubMed: 15879138]  [MGI Ref ID J:99044]

Held K; Thiel S; Loos M; Petry F. 2008. Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol 45(15):3934-41. [PubMed: 18672286]  [MGI Ref ID J:140046]

Hilfiker-Kleiner D; Shukla P; Klein G; Schaefer A; Stapel B; Hoch M; Muller W; Scherr M; Theilmeier G; Ernst M; Hilfiker A; Drexler H. 2010. Continuous glycoprotein-130-mediated signal transducer and activator of transcription-3 activation promotes inflammation, left ventricular rupture, and adverse outcome in subacute myocardial infarction. Circulation 122(2):145-55. [PubMed: 20585009]  [MGI Ref ID J:178593]

Hogaboam CM; Takahashi K; Ezekowitz RA; Kunkel SL; Schuh JM. 2004. Mannose-binding lectin deficiency alters the development of fungal asthma: effects on airway response, inflammation, and cytokine profile. J Leukoc Biol 75(5):805-14. [PubMed: 14761934]  [MGI Ref ID J:89442]

Ip WK; Takahashi K; Moore KJ; Stuart LM; Ezekowitz RA. 2008. Mannose-binding lectin enhances Toll-like receptors 2 and 6 signaling from the phagosome. J Exp Med 205(1):169-81. [PubMed: 18180310]  [MGI Ref ID J:131122]

La Bonte LR; Pavlov VI; Tan YS; Takahashi K; Takahashi M; Banda NK; Zou C; Fujita T; Stahl GL. 2012. Mannose-binding lectin-associated serine protease-1 is a significant contributor to coagulation in a murine model of occlusive thrombosis. J Immunol 188(2):885-91. [PubMed: 22156595]  [MGI Ref ID J:180797]

Mihai S; Chiriac MT; Takahashi K; Thurman JM; Holers VM; Zillikens D; Botto M; Sitaru C. 2007. The alternative pathway of complement activation is critical for blister induction in experimental epidermolysis bullosa acquisita. J Immunol 178(10):6514-21. [PubMed: 17475881]  [MGI Ref ID J:146108]

Miwa T; Sato S; Gullipalli D; Nangaku M; Song WC. 2013. Blocking Properdin, the Alternative Pathway, and Anaphylatoxin Receptors Ameliorates Renal Ischemia-Reperfusion Injury in Decay-Accelerating Factor and CD59 Double-Knockout Mice. J Immunol 190(7):3552-9. [PubMed: 23427256]  [MGI Ref ID J:194520]

Moller-Kristensen M; Hamblin MR; Thiel S; Jensenius JC; Takahashi K. 2007. Burn injury reveals altered phenotype in mannan-binding lectin-deficient mice. J Invest Dermatol 127(6):1524-31. [PubMed: 17363917]  [MGI Ref ID J:121549]

Moller-Kristensen M; Ip WK; Shi L; Gowda LD; Hamblin MR; Thiel S; Jensenius JC; Ezekowitz RA; Takahashi K. 2006. Deficiency of mannose-binding lectin greatly increases susceptibility to postburn infection with Pseudomonas aeruginosa. J Immunol 176(3):1769-75. [PubMed: 16424207]  [MGI Ref ID J:106353]

Moller-Kristensen M; Wang W; Ruseva M; Thiel S; Nielsen S; Takahashi K; Shi L; Ezekowitz A; Jensenius JC; Gadjeva M. 2005. Mannan-binding lectin recognizes structures on ischaemic reperfused mouse kidneys and is implicated in tissue injury. Scand J Immunol 61(5):426-34. [PubMed: 15882434]  [MGI Ref ID J:106351]

Moulton EA; Atkinson JP; Buller RM. 2008. Surviving mousepox infection requires the complement system. PLoS Pathog 4(12):e1000249. [PubMed: 19112490]  [MGI Ref ID J:162201]

Nimmerjahn F; Anthony RM; Ravetch JV. 2007. Agalactosylated IgG antibodies depend on cellular Fc receptors for in vivo activity. Proc Natl Acad Sci U S A 104(20):8433-7. [PubMed: 17485663]  [MGI Ref ID J:121840]

Pavlov VI; La Bonte LR; Baldwin WM; Markiewski MM; Lambris JD; Stahl GL. 2012. Absence of mannose-binding lectin prevents hyperglycemic cardiovascular complications. Am J Pathol 180(1):104-12. [PubMed: 22079428]  [MGI Ref ID J:180214]

Pavlov VI; Skjoedt MO; Siow Tan Y; Rosbjerg A; Garred P; Stahl GL. 2012. Endogenous and natural complement inhibitor attenuates myocardial injury and arterial thrombogenesis. Circulation 126(18):2227-35. [PubMed: 23032324]  [MGI Ref ID J:210067]

Petry F; Jakobi V; Wagner S; Tessema TS; Thiel S; Loos M. 2008. Binding and activation of human and mouse complement by Cryptosporidium parvum (Apicomplexa) and susceptibility of C1q- and MBL-deficient mice to infection. Mol Immunol 45(12):3392-400. [PubMed: 18501966]  [MGI Ref ID J:137680]

Renner B; Strassheim D; Amura CR; Kulik L; Ljubanovic D; Glogowska MJ; Takahashi K; Carroll MC; Holers VM; Thurman JM. 2010. B cell subsets contribute to renal injury and renal protection after ischemia/reperfusion. J Immunol 185(7):4393-400. [PubMed: 20810984]  [MGI Ref ID J:164313]

Rohrer B; Coughlin B; Kunchithapautham K; Long Q; Tomlinson S; Takahashi K; Holers VM. 2011. The alternative pathway is required, but not alone sufficient, for retinal pathology in mouse laser-induced choroidal neovascularization. Mol Immunol 48(6-7):e1-8. [PubMed: 21257205]  [MGI Ref ID J:174154]

Rothfuchs AG; Roffe E; Gibson A; Cheever AW; Ezekowitz RA; Takahashi K; Steindel M; Sher A; Bafica A. 2012. Mannose-binding lectin regulates host resistance and pathology during experimental infection with Trypanosoma cruzi. PLoS One 7(11):e47835. [PubMed: 23139754]  [MGI Ref ID J:194799]

Ruseva M; Kolev M; Dagnaes-Hansen F; Hansen SB; Takahashi K; Ezekowitz A; Thiel S; Jensenius JC; Gadjeva M. 2009. Mannan-binding lectin deficiency modulates the humoral immune response dependent on the genetic environment. Immunology 127(2):279-88. [PubMed: 19476514]  [MGI Ref ID J:155648]

Stuart LM; Takahashi K; Shi L; Savill J; Ezekowitz RA. 2005. Mannose-binding lectin-deficient mice display defective apoptotic cell clearance but no autoimmune phenotype. J Immunol 174(6):3220-6. [PubMed: 15749852]  [MGI Ref ID J:97686]

Takahashi K; Gordon J; Liu H; Sastry KN; Epstein JE; Motwani M; Laursen I; Thiel S; Jensenius JC; Carroll M; Ezekowitz RA. 2002. Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis. Microbes Infect 4(8):773-84. [PubMed: 12270724]  [MGI Ref ID J:90437]

Takahashi K; Kurokawa K; Moyo P; Jung DJ; An JH; Chigweshe L; Paul E; Lee BL. 2013. Intradermal immunization with wall teichoic acid (WTA) elicits and augments an anti-WTA IgG response that protects mice from methicillin-resistant Staphylococcus aureus infection independent of mannose-binding lectin status. PLoS One 8(8):e69739. [PubMed: 23936347]  [MGI Ref ID J:204937]

Takahashi K; Shi L; Gowda LD; Ezekowitz RA. 2005. Relative roles of complement factor 3 and mannose-binding lectin in host defense against infection. Infect Immun 73(12):8188-93. [PubMed: 16299314]  [MGI Ref ID J:104305]

Walsh MC; Bourcier T; Takahashi K; Shi L; Busche MN; Rother RP; Solomon SD; Ezekowitz RA; Stahl GL. 2005. Mannose-binding lectin is a regulator of inflammation that accompanies myocardial ischemia and reperfusion injury. J Immunol 175(1):541-6. [PubMed: 15972690]  [MGI Ref ID J:100604]

Zhang M; Takahashi K; Alicot EM; Vorup-Jensen T; Kessler B; Thiel S; Jensenius JC; Ezekowitz RA; Moore FD; Carroll MC. 2006. Activation of the lectin pathway by natural IgM in a model of ischemia/reperfusion injury. J Immunol 177(7):4727-34. [PubMed: 16982912]  [MGI Ref ID J:139312]

Zhou HF; Yan H; Bertram P; Hu Y; Springer LE; Thompson RW; Curci JA; Hourcade DE; Pham CT. 2013. Fibrinogen-specific antibody induces abdominal aortic aneurysm in mice through complement lectin pathway activation. Proc Natl Acad Sci U S A 110(46):E4335-44. [PubMed: 24167262]  [MGI Ref ID J:202894]

Mbl2tm1Kata related

Ali YM; Lynch NJ; Haleem KS; Fujita T; Endo Y; Hansen S; Holmskov U; Takahashi K; Stahl GL; Dudler T; Girija UV; Wallis R; Kadioglu A; Stover CM; Andrew PW; Schwaeble WJ. 2012. The lectin pathway of complement activation is a critical component of the innate immune response to pneumococcal infection. PLoS Pathog 8(7):e1002793. [PubMed: 22792067]  [MGI Ref ID J:195379]

Banda NK; Levitt B; Wood AK; Takahashi K; Stahl GL; Holers VM; Arend WP. 2009. Complement activation pathways in murine immune complex-induced arthritis and in C3a and C5a generation in vitro. Clin Exp Immunol 159(1):100-8. [PubMed: 19843088]  [MGI Ref ID J:155358]

Banda NK; Takahashi K; Wood AK; Holers VM; Arend WP. 2007. Pathogenic complement activation in collagen antibody-induced arthritis in mice requires amplification by the alternative pathway. J Immunol 179(6):4101-9. [PubMed: 17785849]  [MGI Ref ID J:152026]

Banda NK; Takahashi M; Levitt B; Glogowska M; Nicholas J; Takahashi K; Stahl GL; Fujita T; Arend WP; Holers VM. 2010. Essential role of complement mannose-binding lectin-associated serine proteases-1/3 in the murine collagen antibody-induced model of inflammatory arthritis. J Immunol 185(9):5598-606. [PubMed: 20870940]  [MGI Ref ID J:165200]

Banda NK; Takahashi M; Takahashi K; Stahl GL; Hyatt S; Glogowska M; Wiles TA; Endo Y; Fujita T; Michael Holers V; Arend WP. 2011. Mechanisms of mannose-binding lectin-associated serine proteases-1/3 activation of the alternative pathway of complement. Mol Immunol 49(1-2):281-9. [PubMed: 21943708]  [MGI Ref ID J:177350]

Bidula S; Kenawy H; Ali YM; Sexton D; Schwaeble WJ; Schelenz S. 2013. Role of Ficolin-A and Lectin Complement Pathway in the Innate Defense against Pathogenic Aspergillus Species. Infect Immun 81(5):1730-40. [PubMed: 23478320]  [MGI Ref ID J:194903]

Busche MN; Pavlov V; Takahashi K; Stahl GL. 2009. Myocardial ischemia and reperfusion injury is dependent on both IgM and mannose-binding lectin. Am J Physiol Heart Circ Physiol 297(5):H1853-9. [PubMed: 19749170]  [MGI Ref ID J:154309]

Busche MN; Walsh MC; McMullen ME; Guikema BJ; Stahl GL. 2008. Mannose-binding lectin plays a critical role in myocardial ischaemia and reperfusion injury in a mouse model of diabetes. Diabetologia 51(8):1544-51. [PubMed: 18493734]  [MGI Ref ID J:137894]

Cervera A; Planas AM; Justicia C; Urra X; Jensenius JC; Torres F; Lozano F; Chamorro A. 2010. Genetically-defined deficiency of mannose-binding lectin is associated with protection after experimental stroke in mice and outcome in human stroke. PLoS One 5(2):e8433. [PubMed: 20140243]  [MGI Ref ID J:158006]

Chan RK; Ibrahim SI; Takahashi K; Kwon E; McCormack M; Ezekowitz A; Carroll MC; Moore FD Jr; Austen WG Jr. 2006. The differing roles of the classical and mannose-binding lectin complement pathways in the events following skeletal muscle ischemia-reperfusion. J Immunol 177(11):8080-5. [PubMed: 17114482]  [MGI Ref ID J:140685]

Gadjeva M; Paludan SR; Thiel S; Slavov V; Ruseva M; Eriksson K; Lowhagen GB; Shi L; Takahashi K; Ezekowitz A; Jensenius JC. 2004. Mannan-binding lectin modulates the response to HSV-2 infection. Clin Exp Immunol 138(2):304-11. [PubMed: 15498041]  [MGI Ref ID J:106352]

Gonzalez SF; Lukacs-Kornek V; Kuligowski MP; Pitcher LA; Degn SE; Kim YA; Cloninger MJ; Martinez-Pomares L; Gordon S; Turley SJ; Carroll MC. 2010. Capture of influenza by medullary dendritic cells via SIGN-R1 is essential for humoral immunity in draining lymph nodes. Nat Immunol 11(5):427-34. [PubMed: 20305659]  [MGI Ref ID J:158970]

Gunn BM; Morrison TE; Whitmore AC; Blevins LK; Hueston L; Fraser RJ; Herrero LJ; Ramirez R; Smith PN; Mahalingam S; Heise MT. 2012. Mannose binding lectin is required for alphavirus-induced arthritis/myositis. PLoS Pathog 8(3):e1002586. [PubMed: 22457620]  [MGI Ref ID J:195398]

Guttormsen HK; Stuart LM; Shi L; Carroll MC; Chen J; Kasper DL; Ezekowitz RA; Takahashi K. 2009. Deficiency of mannose-binding lectin greatly increases antibody response in a mouse model of vaccination. Clin Immunol 130(3):264-71. [PubMed: 18996748]  [MGI Ref ID J:145190]

Hart ML; Ceonzo KA; Shaffer LA; Takahashi K; Rother RP; Reenstra WR; Buras JA; Stahl GL. 2005. Gastrointestinal ischemia-reperfusion injury is lectin complement pathway dependent without involving C1q. J Immunol 174(10):6373-80. [PubMed: 15879138]  [MGI Ref ID J:99044]

Held K; Thiel S; Loos M; Petry F. 2008. Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol 45(15):3934-41. [PubMed: 18672286]  [MGI Ref ID J:140046]

Hilfiker-Kleiner D; Shukla P; Klein G; Schaefer A; Stapel B; Hoch M; Muller W; Scherr M; Theilmeier G; Ernst M; Hilfiker A; Drexler H. 2010. Continuous glycoprotein-130-mediated signal transducer and activator of transcription-3 activation promotes inflammation, left ventricular rupture, and adverse outcome in subacute myocardial infarction. Circulation 122(2):145-55. [PubMed: 20585009]  [MGI Ref ID J:178593]

Ip WK; Takahashi K; Moore KJ; Stuart LM; Ezekowitz RA. 2008. Mannose-binding lectin enhances Toll-like receptors 2 and 6 signaling from the phagosome. J Exp Med 205(1):169-81. [PubMed: 18180310]  [MGI Ref ID J:131122]

La Bonte LR; Pavlov VI; Tan YS; Takahashi K; Takahashi M; Banda NK; Zou C; Fujita T; Stahl GL. 2012. Mannose-binding lectin-associated serine protease-1 is a significant contributor to coagulation in a murine model of occlusive thrombosis. J Immunol 188(2):885-91. [PubMed: 22156595]  [MGI Ref ID J:180797]

Mihai S; Chiriac MT; Takahashi K; Thurman JM; Holers VM; Zillikens D; Botto M; Sitaru C. 2007. The alternative pathway of complement activation is critical for blister induction in experimental epidermolysis bullosa acquisita. J Immunol 178(10):6514-21. [PubMed: 17475881]  [MGI Ref ID J:146108]

Miwa T; Sato S; Gullipalli D; Nangaku M; Song WC. 2013. Blocking Properdin, the Alternative Pathway, and Anaphylatoxin Receptors Ameliorates Renal Ischemia-Reperfusion Injury in Decay-Accelerating Factor and CD59 Double-Knockout Mice. J Immunol 190(7):3552-9. [PubMed: 23427256]  [MGI Ref ID J:194520]

Moller-Kristensen M; Hamblin MR; Thiel S; Jensenius JC; Takahashi K. 2007. Burn injury reveals altered phenotype in mannan-binding lectin-deficient mice. J Invest Dermatol 127(6):1524-31. [PubMed: 17363917]  [MGI Ref ID J:121549]

Moller-Kristensen M; Ip WK; Shi L; Gowda LD; Hamblin MR; Thiel S; Jensenius JC; Ezekowitz RA; Takahashi K. 2006. Deficiency of mannose-binding lectin greatly increases susceptibility to postburn infection with Pseudomonas aeruginosa. J Immunol 176(3):1769-75. [PubMed: 16424207]  [MGI Ref ID J:106353]

Moller-Kristensen M; Wang W; Ruseva M; Thiel S; Nielsen S; Takahashi K; Shi L; Ezekowitz A; Jensenius JC; Gadjeva M. 2005. Mannan-binding lectin recognizes structures on ischaemic reperfused mouse kidneys and is implicated in tissue injury. Scand J Immunol 61(5):426-34. [PubMed: 15882434]  [MGI Ref ID J:106351]

Moulton EA; Atkinson JP; Buller RM. 2008. Surviving mousepox infection requires the complement system. PLoS Pathog 4(12):e1000249. [PubMed: 19112490]  [MGI Ref ID J:162201]

Pavlov VI; La Bonte LR; Baldwin WM; Markiewski MM; Lambris JD; Stahl GL. 2012. Absence of mannose-binding lectin prevents hyperglycemic cardiovascular complications. Am J Pathol 180(1):104-12. [PubMed: 22079428]  [MGI Ref ID J:180214]

Pavlov VI; Skjoedt MO; Siow Tan Y; Rosbjerg A; Garred P; Stahl GL. 2012. Endogenous and natural complement inhibitor attenuates myocardial injury and arterial thrombogenesis. Circulation 126(18):2227-35. [PubMed: 23032324]  [MGI Ref ID J:210067]

Petry F; Jakobi V; Wagner S; Tessema TS; Thiel S; Loos M. 2008. Binding and activation of human and mouse complement by Cryptosporidium parvum (Apicomplexa) and susceptibility of C1q- and MBL-deficient mice to infection. Mol Immunol 45(12):3392-400. [PubMed: 18501966]  [MGI Ref ID J:137680]

Renner B; Strassheim D; Amura CR; Kulik L; Ljubanovic D; Glogowska MJ; Takahashi K; Carroll MC; Holers VM; Thurman JM. 2010. B cell subsets contribute to renal injury and renal protection after ischemia/reperfusion. J Immunol 185(7):4393-400. [PubMed: 20810984]  [MGI Ref ID J:164313]

Rohrer B; Coughlin B; Kunchithapautham K; Long Q; Tomlinson S; Takahashi K; Holers VM. 2011. The alternative pathway is required, but not alone sufficient, for retinal pathology in mouse laser-induced choroidal neovascularization. Mol Immunol 48(6-7):e1-8. [PubMed: 21257205]  [MGI Ref ID J:174154]

Rothfuchs AG; Roffe E; Gibson A; Cheever AW; Ezekowitz RA; Takahashi K; Steindel M; Sher A; Bafica A. 2012. Mannose-binding lectin regulates host resistance and pathology during experimental infection with Trypanosoma cruzi. PLoS One 7(11):e47835. [PubMed: 23139754]  [MGI Ref ID J:194799]

Ruseva M; Kolev M; Dagnaes-Hansen F; Hansen SB; Takahashi K; Ezekowitz A; Thiel S; Jensenius JC; Gadjeva M. 2009. Mannan-binding lectin deficiency modulates the humoral immune response dependent on the genetic environment. Immunology 127(2):279-88. [PubMed: 19476514]  [MGI Ref ID J:155648]

Stuart LM; Takahashi K; Shi L; Savill J; Ezekowitz RA. 2005. Mannose-binding lectin-deficient mice display defective apoptotic cell clearance but no autoimmune phenotype. J Immunol 174(6):3220-6. [PubMed: 15749852]  [MGI Ref ID J:97686]

Takahashi K; Kurokawa K; Moyo P; Jung DJ; An JH; Chigweshe L; Paul E; Lee BL. 2013. Intradermal immunization with wall teichoic acid (WTA) elicits and augments an anti-WTA IgG response that protects mice from methicillin-resistant Staphylococcus aureus infection independent of mannose-binding lectin status. PLoS One 8(8):e69739. [PubMed: 23936347]  [MGI Ref ID J:204937]

Takahashi K; Shi L; Gowda LD; Ezekowitz RA. 2005. Relative roles of complement factor 3 and mannose-binding lectin in host defense against infection. Infect Immun 73(12):8188-93. [PubMed: 16299314]  [MGI Ref ID J:104305]

Walsh MC; Bourcier T; Takahashi K; Shi L; Busche MN; Rother RP; Solomon SD; Ezekowitz RA; Stahl GL. 2005. Mannose-binding lectin is a regulator of inflammation that accompanies myocardial ischemia and reperfusion injury. J Immunol 175(1):541-6. [PubMed: 15972690]  [MGI Ref ID J:100604]

Zhou HF; Yan H; Bertram P; Hu Y; Springer LE; Thompson RW; Curci JA; Hourcade DE; Pham CT. 2013. Fibrinogen-specific antibody induces abdominal aortic aneurysm in mice through complement lectin pathway activation. Proc Natl Acad Sci U S A 110(46):E4335-44. [PubMed: 24167262]  [MGI Ref ID J:202894]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes at both loci.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-AUG-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Mbl1tm1Kata, Homozygous for Mbl2tm1Kata  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Mbl1tm1Kata, Homozygous for Mbl2tm1Kata x Homozygous for Mbl1tm1Kata, Homozygous for Mbl2tm1Kata  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Mbl1tm1Kata, Homozygous for Mbl2tm1Kata  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Mbl1tm1Kata, Homozygous for Mbl2tm1Kata x Homozygous for Mbl1tm1Kata, Homozygous for Mbl2tm1Kata  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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