Strain Name: |
STOCK Tg(Zp3-EGFP)1Dean/J |
|---|---|
Stock Number: |
006129 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; Zp3; Tg(Zp3-EGFP)1Dean; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Jurrien Dean, NIH Generation ?+F1 (23-MAR-07) Strain Description
Hemizygous mice are viable and fertile with no gross phenotypic abnormalities. These mice express the Zp3-EGFP fusion protein under the direction of the Zp3 promoter. As such, EGFP is readily detected throughout the width of the zona pellucida surrounding growing oocytes, with no expression in the surrounding somatic cells. Mutant mice have normal synthesis, intracellular trafficking, and secretion/incorporation into the zona pellucida matrix of the transgenic fusion protein. These mice may be useful in studies of reproductive biology, including intracellular trafficking of zona pellucida proteins to the cell surface, oocyte development, fertilization research, as well as developmental biology.Strain Development
An enhanced green fluorescent protein (Clonetech) gene was inserted in-frame into a murine Zp3 cDNA sequence (1-1189 bp) immediately after base pair 149 (just downstream of the of the N-terminal signal peptide). This entire construct was placed under the control of the mouse Zp3 promoter. The resulting transgene was injected into FVB embryos. Embryos were transferred into the oviducts of pseudopregnant foster mothers. Transgenic offspring (founder line 1) were then backcrossed to wildtype (either CF-1 or FVB) mice for less than 5 generations. Next, transgenic mice were bred together for many generations (optimizing the breeders by utilizing test crosses) prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Zp3-EGFP)1Dean | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jurrien Dean | ||
| Common Name(s) | EGFP-MoZP3; EGFP-ZP3; EGFP-ZP3 (normal furin site); normal ZP3-EGFP; | ||
| Mutation Made By | Jurrien Dean, NIH | ||
| Strain of Origin | FVB | ||
| Site of Expression | EGFP is readily detected throughout the width of the zona pellucida surrounding growing oocytes. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Zp3, zona pellucida glycoprotein 3, mouse, laboratory | ||
| Molecular Note | The ~8.1 kb transgene comprises the mouse Zp3 cDNA into which the the enhanced green fluorescent protein (EGFP) cDNA has been inserted downstream of the sequence encoding the 40 amino acid N-terminal signal peptide. Expression of the fusion gene is driven by ~6 kb of the oocyte-specific mouse Zp3 promoter. Ovulated ova and growing oocytes from transgenic mice exhibit EGFP fluorescence throughout the matrix of the zona pellucida; surrounding somatic cells do not fluoresce. Mature oocytes exhibit the strongest signal. Smaller, growing oocytes contain large, circular structures whose boundaries fluoresce and costain with BODIPY-Texas red ceramide. [MGI Ref ID J:78932] [MGI Ref ID J:86870] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice are bred together or to wildtype siblings. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
004038 B6.129S4-Zp3tm1Dean/J 006888 C57BL/6-Tg(Zp3-cre)1Gwh/J 003394 C57BL/6-Tg(Zp3-cre)3Mrt/J 003651 C57BL/6-Tg(Zp3-cre)93Knw/J 003377 FVB/N-Tg(Zp3-cre)3Mrt/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedReproductive Biology Research
Developmental Defects Affecting Gonads (parthenogenic activation)
Fertility Defects
Research Tools
Developmental Biology Research
Fluorescent Proteins
Reproductive Biology Research (oocyte)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesZhao M; Gold L; Ginsberg AM; Liang LF; Dean J. 2002. Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice. Mol Cell Biol 22(9):3111-20. [PubMed: 11940668] [MGI Ref ID J:78932]
Price and Supply Information
| Strain Name: | STOCK Tg(Zp3-EGFP)1Dean/J |
| Stock Number: | 006129 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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