Strain Name:

C.129S4(B6)-Cxcl10tm1Adl/J

Stock Number:

006134

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation?+F2p
Generation Definitions
 
Donating Investigator Andrew D Luster,   Massachusetts General Hospital-East

Description
Homozygous mice are viable, fertile, and have no overt morphological or developmental abnormalities. No endogenous gene expression is observed in bone marrow-derived macrophages before or after IFN-gamma stimulation. Homozygous mice have defective T cell responses, including impaired proliferation and IFN-gamma secretion following antigenic challenge (129Sv background). In experimental models of T helper-1 (Th1)-mediated immune responses, homozygous-deletion leads to diminished immune function; contact hypersensitivity is reduced (129Sv background) and diminished threshold for disease expression in experimental autoimmune encephalomyelitis (EAE, human model of multiple sclerosis) (C57BL/6 background). After injection with a neurotropic coronavirus MHV, null mice (on a B6;129Sv background) exhibit impaired viral clearance, decreased CD4+/CD8+ infiltration into the brain, and are protected from viral-induced demyelination. Similarly, homozygous mice (on a C57BL/6 background) infected with West Nile Virus have increased viral load in brain, altered CD8+ effector T cell recruitment to neurons and increased mortality. These mutant mice may be useful in studies of Th1-type inflammatory disease, chemokine biology, and T cell priming, proliferation, and trafficking.

In an attempt to offer allele s on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector containing a mouse phosphoglycerate kinase promoter driven neomycin resistance gene was used to replace exons 1-3 of the endogenous gene. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to C57BL/6 generate a mutant colony. Mutant mice were then mated to BALB/c for 9 generations and then made homozygous before arriving at The Jackson Laboratory.

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Cxcl10tm1Adl allele
006087   B6.129S4-Cxcl10tm1Adl/J
View Strains carrying   Cxcl10tm1Adl     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Cxcl10tm1Adl/Cxcl10tm1Adl

        involves: 129S4/SvJae * 129X1/SvJ
  • immune system phenotype
  • decreased IgG2a level
    • reduced levels of antigen specific IgG2a in serum when immunized with OVA   (MGI Ref ID J:75584)
  • decreased interferon-gamma secretion
    • secretion of IFN-gamma reduced 60%   (MGI Ref ID J:75584)
  • decreased splenocyte proliferation
    • splenocytes display significantly reduced proliferative responses in mixed lymphocyte reactions using MHC antigens but not when using conA   (MGI Ref ID J:75584)
    • proliferative responses after sensitization to specific antigens is also reduced   (MGI Ref ID J:75584)
  • decreased susceptibility to type IV hypersensitivity reaction
    • reduced contact hypersensitivity   (MGI Ref ID J:75584)
  • hematopoietic system phenotype
  • decreased IgG2a level
    • reduced levels of antigen specific IgG2a in serum when immunized with OVA   (MGI Ref ID J:75584)
  • decreased splenocyte proliferation
    • splenocytes display significantly reduced proliferative responses in mixed lymphocyte reactions using MHC antigens but not when using conA   (MGI Ref ID J:75584)
    • proliferative responses after sensitization to specific antigens is also reduced   (MGI Ref ID J:75584)
  • cellular phenotype
  • decreased splenocyte proliferation
    • splenocytes display significantly reduced proliferative responses in mixed lymphocyte reactions using MHC antigens but not when using conA   (MGI Ref ID J:75584)
    • proliferative responses after sensitization to specific antigens is also reduced   (MGI Ref ID J:75584)

Cxcl10tm1Adl/Cxcl10tm1Adl

        involves: 129S4/SvJae * C57BL/6
  • immune system phenotype
  • abnormal T cell physiology
    • decreased CNS levels of both CD4+ and CD8+ T cells after mouse hepatitis virus infection   (MGI Ref ID J:75584)
  • abnormal interferon level
    • decreased IFN-gamma in response to mouse hepatitis virus   (MGI Ref ID J:75584)
  • decreased CD8-positive, alpha-beta T cell number
    • 25% decrease in the spleen after mouse hepatitis virus infection   (MGI Ref ID J:75584)
  • increased susceptibility to viral infection
    • greater sensitivity to mouse hepatitis virus   (MGI Ref ID J:75584)
    • titers similar to controls after 7 days but significantly higher than controls 12 days after infection   (MGI Ref ID J:75584)
  • nervous system phenotype
  • demyelination
    • level of demyelination reduced 12 days after infection with mouse hepatitis virus   (MGI Ref ID J:75584)
  • hematopoietic system phenotype
  • abnormal T cell physiology
    • decreased CNS levels of both CD4+ and CD8+ T cells after mouse hepatitis virus infection   (MGI Ref ID J:75584)
  • decreased CD8-positive, alpha-beta T cell number
    • 25% decrease in the spleen after mouse hepatitis virus infection   (MGI Ref ID J:75584)
  • homeostasis/metabolism phenotype
  • abnormal interferon level
    • decreased IFN-gamma in response to mouse hepatitis virus   (MGI Ref ID J:75584)

Cxcl10tm1Adl/Cxcl10tm1Adl

        B6.129S4-Cxcl10tm1Adl
  • immune system phenotype
  • CNS inflammation
    • experimental autoimmune encephalomyelitis involves meningeal and brain parenchyma after 15 days   (MGI Ref ID J:87076)
    • monocytes lymphocytes and some granulocytes are involved   (MGI Ref ID J:87076)
    • increased levels of IFN-gamma, Il-2 and TGF-Beta1 in lymph nodes   (MGI Ref ID J:87076)
  • abnormal cytokine secretion
    • only CCL2 levels are elevated in corneas relative to wild-type or Cxcl9-null mice after HSV-1 infection   (MGI Ref ID J:120743)
  • increased susceptibility to experimental autoimmune encephalomyelitis
    • higher incidence and greater severity of experimental autoimmune encephalomyelitis   (MGI Ref ID J:87076)
  • increased susceptibility to viral infection
    • 7 days after infection (day 7 pi) with herpes simplex virus type I (HSV-1), homozygotes have significantly higher HSV-1 titers in the cornea compared to Cxcl9-null or wild-type mice   (MGI Ref ID J:120743)
    • virus shedding in the tear film is elevated at day 7 pi but not at early points relative to Cxcl9 homozygotes or wild-type controls   (MGI Ref ID J:120743)
    • mice usually succumb to infection between days 7 and 9 pi   (MGI Ref ID J:120743)
  • nervous system phenotype
  • CNS inflammation
    • experimental autoimmune encephalomyelitis involves meningeal and brain parenchyma after 15 days   (MGI Ref ID J:87076)
    • monocytes lymphocytes and some granulocytes are involved   (MGI Ref ID J:87076)
    • increased levels of IFN-gamma, Il-2 and TGF-Beta1 in lymph nodes   (MGI Ref ID J:87076)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      experimental allergic encephalomyelitis (EAE)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      genes regulating susceptibility to infectious disease and endotoxin
Growth Factors/Receptors/Cytokines
Immunodeficiency
      T cell deficiency
      specific T cell deficiency
Immunodeficiency Associated with Other Defects

Research Tools
Cell Biology Research
Immunology, Inflammation and Autoimmunity Research
      T cell deficiency
      genes regulating susceptibility to infectious disease and endotoxin
      specific T cell deficiency

Virology Research
B and T Cell Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cxcl10tm1Adl
Allele Name targeted mutation 1, Andrew D Luster
Allele Type Targeted (Null/Knockout)
Common Name(s) IP-10-;
Mutation Made By Andrew Luster,   Massachusetts General Hospital-East
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Cxcl10, chemokine (C-X-C motif) ligand 10
Chromosome 5
Gene Common Name(s) C7; CRG-2; IFI10; INP10; IP-10; IP10; Ifi10; SCYB10; Scyb10; gIP-10; interferon activated gene 10; mob-1; small inducible cytokine B subfamily (Cys-X-Cys), member 10;
Molecular Note Exons 1-3, which encompass most of the coding region of the gene, were replaced with a PGK-neo cassette via homologous recombination. Northern and Western blot analyses of bone marrow macrophages confirmed the absence of gene expression in homozygous mutant animals. [MGI Ref ID J:75584]

Genotyping

Genotyping Information

Genotyping Protocols

Cxcl10tm1Adl, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Cxcl10tm1Adl related

Auerbach MB; Shimoda N; Amano H; Rosenblum JM; Kish DD; Farber JM; Fairchild RL. 2009. Monokine induced by interferon-gamma (MIG/CXCL9) is derived from both donor and recipient sources during rejection of class II major histocompatibility complex disparate skin allografts. Am J Pathol 174(6):2172-81. [PubMed: 19389928]  [MGI Ref ID J:148781]

Bujak M; Dobaczewski M; Gonzalez-Quesada C; Xia Y; Leucker T; Zymek P; Veeranna V; Tager AM; Luster AD; Frangogiannis NG. 2009. Induction of the CXC chemokine interferon-gamma-inducible protein 10 regulates the reparative response following myocardial infarction. Circ Res 105(10):973-83. [PubMed: 19797174]  [MGI Ref ID J:169950]

Campanella GS; Tager AM; El Khoury JK; Thomas SY; Abrazinski TA; Manice LA; Colvin RA; Luster AD. 2008. Chemokine receptor CXCR3 and its ligands CXCL9 and CXCL10 are required for the development of murine cerebral malaria. Proc Natl Acad Sci U S A 105(12):4814-9. [PubMed: 18347328]  [MGI Ref ID J:133359]

Christensen JE; Simonsen S; Fenger C; Sorensen MR; Moos T; Christensen JP; Finsen B; Thomsen AR. 2009. Fulminant lymphocytic choriomeningitis virus-induced inflammation of the CNS involves a cytokine-chemokine-cytokine-chemokine cascade. J Immunol 182(2):1079-87. [PubMed: 19124751]  [MGI Ref ID J:143550]

Christensen JE; de Lemos C; Moos T; Christensen JP; Thomsen AR. 2006. CXCL10 is the key ligand for CXCR3 on CD8+ effector T cells involved in immune surveillance of the lymphocytic choriomeningitis virus-infected central nervous system. J Immunol 176(7):4235-43. [PubMed: 16547260]  [MGI Ref ID J:129874]

Coppieters KT; Amirian N; Pagni PP; Baca Jones C; Wiberg A; Lasch S; Hintermann E; Christen U; von Herrath MG. 2013. Functional redundancy of CXCR3/CXCL10 signaling in the recruitment of diabetogenic cytotoxic T lymphocytes to pancreatic islets in a virally induced autoimmune diabetes model. Diabetes 62(7):2492-9. [PubMed: 23434930]  [MGI Ref ID J:208545]

Craig VJ; Quintero PA; Fyfe SE; Patel AS; Knolle MD; Kobzik L; Owen CA. 2013. Profibrotic Activities for Matrix Metalloproteinase-8 during Bleomycin-Mediated Lung Injury. J Immunol 190(8):4283-96. [PubMed: 23487425]  [MGI Ref ID J:195119]

Dufour JH; Dziejman M; Liu MT; Leung JH; Lane TE; Luster AD. 2002. IFN-gamma-inducible protein 10 (IP-10; CXCL10)-deficient mice reveal a role for IP-10 in effector T cell generation and trafficking. J Immunol 168(7):3195-204. [PubMed: 11907072]  [MGI Ref ID J:75584]

Fujita M; Zhu X; Ueda R; Sasaki K; Kohanbash G; Kastenhuber ER; McDonald HA; Gibson GA; Watkins SC; Muthuswamy R; Kalinski P; Okada H. 2009. Effective immunotherapy against murine gliomas using type 1 polarizing dendritic cells--significant roles of CXCL10. Cancer Res 69(4):1587-95. [PubMed: 19190335]  [MGI Ref ID J:146507]

Gandhapudi SK; Chilton PM; Mitchell TC. 2013. TRIF is required for TLR4 mediated adjuvant effects on T cell clonal expansion. PLoS One 8(2):e56855. [PubMed: 23457630]  [MGI Ref ID J:197179]

Groom JR; Richmond J; Murooka TT; Sorensen EW; Sung JH; Bankert K; von Andrian UH; Moon JJ; Mempel TR; Luster AD. 2012. CXCR3 Chemokine Receptor-Ligand Interactions in the Lymph Node Optimize CD4(+) T Helper 1 Cell Differentiation. Immunity 37(6):1091-103. [PubMed: 23123063]  [MGI Ref ID J:191089]

Hamrah P; Yamagami S; Liu Y; Zhang Q; Vora SS; Lu B; Gerard CJ; Dana MR. 2007. Deletion of the chemokine receptor CCR1 prolongs corneal allograft survival. Invest Ophthalmol Vis Sci 48(3):1228-36. [PubMed: 17325167]  [MGI Ref ID J:123260]

Heller EA; Liu E; Tager AM; Yuan Q; Lin AY; Ahluwalia N; Jones K; Koehn SL; Lok VM; Aikawa E; Moore KJ; Luster AD; Gerszten RE. 2006. Chemokine CXCL10 promotes atherogenesis by modulating the local balance of effector and regulatory T cells. Circulation 113(19):2301-12. [PubMed: 16682613]  [MGI Ref ID J:122449]

Hsieh MF; Lai SL; Chen JP; Sung JM; Lin YL; Wu-Hsieh BA; Gerard C; Luster A; Liao F. 2006. Both CXCR3 and CXCL10/IFN-inducible protein 10 are required for resistance to primary infection by dengue virus. J Immunol 177(3):1855-63. [PubMed: 16849497]  [MGI Ref ID J:137997]

Ip PP; Liao F. 2010. Resistance to dengue virus infection in mice is potentiated by CXCL10 and is independent of CXCL10-mediated leukocyte recruitment. J Immunol 184(10):5705-14. [PubMed: 20400703]  [MGI Ref ID J:160993]

Israelsson C; Bengtsson H; Lobell A; Nilsson LN; Kylberg A; Isaksson M; Wootz H; Lannfelt L; Kullander K; Hillered L; Ebendal T. 2010. Appearance of Cxcl10-expressing cell clusters is common for traumatic brain injury and neurodegenerative disorders. Eur J Neurosci 31(5):852-63. [PubMed: 20374285]  [MGI Ref ID J:159617]

Kastenmuller W; Brandes M; Wang Z; Herz J; Egen JG; Germain RN. 2013. Peripheral Prepositioning and Local CXCL9 Chemokine-Mediated Guidance Orchestrate Rapid Memory CD8(+) T Cell Responses in the Lymph Node. Immunity 38(3):502-13. [PubMed: 23352234]  [MGI Ref ID J:194479]

King VL; Lin AY; Kristo F; Anderson TJ; Ahluwalia N; Hardy GJ; Owens AP 3rd; Howatt DA; Shen D; Tager AM; Luster AD; Daugherty A; Gerszten RE. 2009. Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture. Circulation 119(3):426-35. [PubMed: 19139386]  [MGI Ref ID J:166204]

Klein RS; Izikson L; Means T; Gibson HD; Lin E; Sobel RA; Weiner HL; Luster AD. 2004. IFN-inducible protein 10/CXC chemokine ligand 10-independent induction of experimental autoimmune encephalomyelitis. J Immunol 172(1):550-9. [PubMed: 14688366]  [MGI Ref ID J:87076]

Klein RS; Lin E; Zhang B; Luster AD; Tollett J; Samuel MA; Engle M; Diamond MS. 2005. Neuronal CXCL10 directs CD8+ T-cell recruitment and control of West Nile virus encephalitis. J Virol 79(17):11457-66. [PubMed: 16103196]  [MGI Ref ID J:101771]

Lalor SJ; Segal BM. 2013. Th1-mediated experimental autoimmune encephalomyelitis is CXCR3 independent. Eur J Immunol 43(11):2866-74. [PubMed: 23873018]  [MGI Ref ID J:203012]

Lee JH; Kim HN; Kim KO; Jin WJ; Lee S; Kim HH; Ha H; Lee ZH. 2012. CXCL10 promotes osteolytic bone metastasis by enhancing cancer outgrowth and osteoclastogenesis. Cancer Res 72(13):3175-86. [PubMed: 22562465]  [MGI Ref ID J:189315]

Liu M; Amodu AS; Pitts S; Patrickson J; Hibbert JM; Battle M; Ofori-Acquah SF; Stiles JK. 2012. Heme mediated STAT3 activation in severe malaria. PLoS One 7(3):e34280. [PubMed: 22479586]  [MGI Ref ID J:187123]

Medoff BD; Sauty A; Tager AM; Maclean JA; Smith RN; Mathew A; Dufour JH; Luster AD. 2002. IFN-gamma-inducible protein 10 (CXCL10) contributes to airway hyperreactivity and airway inflammation in a mouse model of asthma. J Immunol 168(10):5278-86. [PubMed: 11994485]  [MGI Ref ID J:125569]

Medoff BD; Wain JC; Seung E; Jackobek R; Means TK; Ginns LC; Farber JM; Luster AD. 2006. CXCR3 and its ligands in a murine model of obliterative bronchiolitis: regulation and function. J Immunol 176(11):7087-95. [PubMed: 16709871]  [MGI Ref ID J:131767]

Peng W; Liu C; Xu C; Lou Y; Chen J; Yang Y; Yagita H; Overwijk WW; Lizee G; Radvanyi L; Hwu P. 2012. PD-1 blockade enhances T-cell migration to tumors by elevating IFN-gamma inducible chemokines. Cancer Res 72(20):5209-18. [PubMed: 22915761]  [MGI Ref ID J:191805]

Pociask DA; Chen K; Mi Choi S; Oury TD; Steele C; Kolls JK. 2011. gammadelta T Cells Attenuate Bleomycin-Induced Fibrosis through the Production of CXCL10. Am J Pathol 178(3):1167-76. [PubMed: 21356368]  [MGI Ref ID J:169690]

Rashighi M; Agarwal P; Richmond JM; Harris TH; Dresser K; Su MW; Zhou Y; Deng A; Hunter CA; Luster AD; Harris JE. 2014. CXCL10 is critical for the progression and maintenance of depigmentation in a mouse model of vitiligo. Sci Transl Med 6(223):223ra23. [PubMed: 24523323]  [MGI Ref ID J:213685]

Rosenblum JM; Shimoda N; Schenk AD; Zhang H; Kish DD; Keslar K; Farber JM; Fairchild RL. 2010. CXC chemokine ligand (CXCL) 9 and CXCL10 are antagonistic costimulation molecules during the priming of alloreactive T cell effectors. J Immunol 184(7):3450-60. [PubMed: 20194716]  [MGI Ref ID J:160084]

Shen FH; Wang SW; Yeh TM; Tung YY; Hsu SM; Chen SH. 2013. Absence of CXCL10 aggravates herpes stromal keratitis with reduced primary neutrophil influx in mice. J Virol 87(15):8502-10. [PubMed: 23720717]  [MGI Ref ID J:200014]

Stiles LN; Hardison JL; Schaumburg CS; Whitman LM; Lane TE. 2006. T cell antiviral effector function is not dependent on CXCL10 following murine coronavirus infection. J Immunol 177(12):8372-80. [PubMed: 17142734]  [MGI Ref ID J:140673]

Tager AM; Kradin RL; LaCamera P; Bercury SD; Campanella GS; Leary CP; Polosukhin V; Zhao LH; Sakamoto H; Blackwell TS; Luster AD. 2004. Inhibition of pulmonary fibrosis by the chemokine IP-10/CXCL10. Am J Respir Cell Mol Biol 31(4):395-404. [PubMed: 15205180]  [MGI Ref ID J:133071]

Thapa M; Carr DJ. 2008. Herpes simplex virus type 2-induced mortality following genital infection is blocked by anti-tumor necrosis factor alpha antibody in CXCL10-deficient mice. J Virol 82(20):10295-301. [PubMed: 18684827]  [MGI Ref ID J:140202]

Thapa M; Welner RS; Pelayo R; Carr DJ. 2008. CXCL9 and CXCL10 expression are critical for control of genital herpes simplex virus type 2 infection through mobilization of HSV-specific CTL and NK cells to the nervous system. J Immunol 180(2):1098-106. [PubMed: 18178850]  [MGI Ref ID J:130943]

Wuest T; Farber J; Luster A; Carr DJ. 2006. CD4+ T cell migration into the cornea is reduced in CXCL9 deficient but not CXCL10 deficient mice following herpes simplex virus type 1 infection. Cell Immunol 243(2):83-9. [PubMed: 17296171]  [MGI Ref ID J:120743]

Wuest TR; Carr DJ. 2008. Dysregulation of CXCR3 signaling due to CXCL10 deficiency impairs the antiviral response to herpes simplex virus 1 infection. J Immunol 181(11):7985-93. [PubMed: 19017990]  [MGI Ref ID J:142374]

Zhang B; Chan YK; Lu B; Diamond MS; Klein RS. 2008. CXCR3 mediates region-specific antiviral T cell trafficking within the central nervous system during West Nile virus encephalitis. J Immunol 180(4):2641-9. [PubMed: 18250476]  [MGI Ref ID J:131972]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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