Strain Name: |
B6.Cg-Tg(Cdh5-cre)7Mlia/J |
|---|---|
Stock Number: |
006137 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Cdh5; cre; Tg(Cdh5-cre)7Mlia; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator M. Luisa Iruela-Arispe, University of California, Los Angeles Generation N12+F3 (23-JUL-08) Strain Description
Hemizygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages.Strain Development
A recombinant vector was designed to place Cre recombinase cDNA downstream from a 2.5-kb fragment of the VE-Cadherin (Cdh5) mouse promoter. This construct was microinjected into FVB/N fertilized oocytes. A selected founder line ("VEN7-CRE") was established and then backcrossed to C57BL/6 for 12 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Cdh5-cre)7Mlia | ||
|---|---|---|---|
| Allele Name | transgene insertion 7, M Luisa Iruela-Arispe | ||
| Common Name(s) | Tg(Cdh5-cre)1Mlia; VE-Cadherin-Cre; VEN7-CRE; | ||
| Mutation Made By | M. Luisa Iruela-Arispe, University of California, Los Angeles | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Embryonic and adult expression in endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cdh5, cadherin 5, mouse, laboratory | ||
| Molecular Note | The cadherin 5 promoter was used to drive expression of cre recombinase in the adult epithelium. Three lines were created. [MGI Ref ID J:106155] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Generic Cre QPCR
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice can be bred to wildtype siblings, C57BL/6J, or bred together to generate homozygous colonies. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (115 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedCardiovascular Research
Vascular Defects
Developmental Biology Research
Internal/Organ Defects (hematopoietic defects)
Internal/Organ Defects (vasculature)
Hematological Research
Hematopoietic Defects
Research Tools
Cancer Research (endothelial cell marker for neovascularization)
Cardiovascular Research (Cre-lox System)
Cardiovascular Research (endothelial cell marker for neovascularization)
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Genetics Research (Tissue/Cell Markers: endothelial cell markers for neovascularization)
Hematological Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesAlva JA; Zovein AC; Monvoisin A; Murphy T; Salazar A; Harvey NL; Carmeliet P; Iruela-Arispe ML. 2006. VE-Cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells. Dev Dyn 235(3):759-67. [PubMed: 16450386] [MGI Ref ID J:106155]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Cdh5-cre)7Mlia/J |
| Stock Number: | 006137 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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