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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 05-SEP-08 Species laboratory mouse Generation N12+F4 (30-DEC-08) Donating Investigator M. Luisa Iruela-Arispe, University of California, Los Angeles Description
Hemizygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages.Development
A recombinant vector was designed to place Cre recombinase cDNA downstream from a 2.5-kb fragment of the VE-Cadherin (Cdh5) mouse promoter. This construct was microinjected into FVB/N fertilized oocytes. A selected founder line ("VEN7-CRE") was established and then backcrossed to C57BL/6 for 12 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Cdh5-cre)7Mlia/0
involves: FVB/N
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:106155)
- mice are viable and fertile; no phenotypic abnormalities are detected
This mouse can be used to support research in many areas including:
cre relatedCardiovascular Research
Vascular Defects
Developmental Biology Research
Internal/Organ Defects
hematopoietic defects
vasculature
Hematological Research
Hematopoietic Defects
Research Tools
Cancer Research
endothelial cell marker for neovascularization
Cardiovascular Research
Cre-lox System
endothelial cell marker for neovascularization
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: endothelial cell markers for neovascularization
Hematological Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Cdh5-cre)7Mlia | ||
|---|---|---|---|
| Allele Name | transgene insertion 7, M Luisa Iruela-Arispe | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(Cdh5-cre)1Mlia; VE-Cadherin-Cre; VEC-Cre; VEN7-CRE; | ||
| Mutation Made By | M. Luisa Iruela-Arispe, University of California, Los Angeles | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Embryonic and adult expression in endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cdh5, cadherin 5, mouse, laboratory | ||
| Driver Note | Cdh5 | ||
| Molecular Note | The cadherin 5 promoter was used to drive expression of cre recombinase in the adult epithelium. Three lines were created. [MGI Ref ID J:106155] | ||
| Gene Symbol and Name | Tg(Cdh5-cre)7Mlia, transgene insertion 7, M Luisa Iruela-Arispe | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Cdh5-cre)1Mlia; VE-Cadherin-Cre; VEC-Cre; VEN7-CRE; transgene insertion 1, M Luisa Iruela-Arispe; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Alva JA; Zovein AC; Monvoisin A; Murphy T; Salazar A; Harvey NL; Carmeliet P; Iruela-Arispe ML. 2006. VE-Cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells. Dev Dyn 235(3):759-67. [PubMed: 16450386] [MGI Ref ID J:106155]
Additional References
Tg(Cdh5-cre)7Mlia relatedEconomopoulou M; Langer HF; Celeste A; Orlova VV; Choi EY; Ma M; Vassilopoulos A; Callen E; Deng C; Bassing CH; Boehm M; Nussenzweig A; Chavakis T. 2009. Histone H2AX is integral to hypoxia-driven neovascularization. Nat Med 15(5):553-8. [PubMed: 19377486] [MGI Ref ID J:149487]
Guo W; Lasky JL; Chang CJ; Mosessian S; Lewis X; Xiao Y; Yeh JE; Chen JY; Iruela-Arispe ML; Varella-Garcia M; Wu H. 2008. Multi-genetic events collaboratively contribute to Pten-null leukaemia stem-cell formation. Nature 453(7194):529-33. [PubMed: 18463637] [MGI Ref ID J:135172]
Harel I; Nathan E; Tirosh-Finkel L; Zigdon H; Guimaraes-Camboa N; Evans SM; Tzahor E. 2009. Distinct origins and genetic programs of head muscle satellite cells. Dev Cell 16(6):822-32. [PubMed: 19531353] [MGI Ref ID J:150129]
Lee S; Chen TT; Barber CL; Jordan MC; Murdock J; Desai S; Ferrara N; Nagy A; Roos KP; Iruela-Arispe ML. 2007. Autocrine VEGF signaling is required for vascular homeostasis. Cell 130(4):691-703. [PubMed: 17719546] [MGI Ref ID J:124900]
Skuli N; Liu L; Runge A; Wang T; Yuan L; Patel S; Iruela-Arispe L; Simon MC; Keith B. 2009. Endothelial deletion of hypoxia-inducible factor-2alpha (HIF-2alpha) alters vascular function and tumor angiogenesis. Blood 114(2):469-77. [PubMed: 19439736] [MGI Ref ID J:150753]
Zovein AC; Hofmann JJ; Lynch M; French WJ; Turlo KA; Yang Y; Becker MS; Zanetta L; Dejana E; Gasson JC; Tallquist MD; Iruela-Arispe ML. 2008. Fate tracing reveals the endothelial origin of hematopoietic stem cells. Cell Stem Cell 3(6):625-36. [PubMed: 19041779] [MGI Ref ID J:149863]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings, C57BL/6J, or bred together to generate homozygous colonies. Mating System Homozygote x Homozygote (Female x Male) 05-SEP-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(Cdh5-cre)7Mlia
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(Cdh5-cre)7Mlia x Homozygous for Tg(Cdh5-cre)7Mlia
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(Cdh5-cre)7Mlia
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(Cdh5-cre)7Mlia x Homozygous for Tg(Cdh5-cre)7Mlia
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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