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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 05-SEP-08 Species laboratory mouse Generation N12+F12 (31-OCT-11)
Generation DefinitionsDonating Investigator M. Luisa Iruela-Arispe, University of California, Los Angeles Description
Hemizygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages.Development
A recombinant vector was designed to place Cre recombinase cDNA downstream from a 2.5-kb fragment of the VE-Cadherin (Cdh5) mouse promoter. This construct was microinjected into FVB/N fertilized oocytes. A selected founder line ("VEN7-CRE") was established and then backcrossed to C57BL/6 for 12 generations prior to sending to The Jackson Laboratory Repository (in 2007). Upon arrival, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. After this, the colony was maintained for several generations by breeding transgenic mice together.In 2011, a 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the rederived living colony at The Jackson Laboratory Repository. This revealed 4 markers that were not C57BL/6 allele-type. Specifically, one marker on chromosome 4 (~20.0 Mbp), two markers on chromosome 8 (~14.8 Mbp and ~15.2 Mbp), and one marker on chromosome 19 (~50.1 Mbp) were homozygous for FVB allele-type. Of note, a marker on chromosome 19 at ~20.5 Mbp is homozygous for C57BL/6 allele-type. These data may suggest possible transgene insertion sites, and/or that the mice were not backcrossed to C57BL/6 for as many generations as originally reported prior to arrival at The Jackson Laboratory Repository.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Cdh5
013585 FVB-Tg(Cdh5-tTA)D5Lbjn/J View Strains carrying other alleles of Cdh5 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Cdh5-cre)7Mlia/0
involves: FVB/N
- normal phenotype
- no abnormal phenotype detected
- mice are viable and fertile; no phenotypic abnormalities are detected (MGI Ref ID J:106155)
This mouse can be used to support research in many areas including:
cre relatedCardiovascular Research
Vascular Defects
Developmental Biology Research
Internal/Organ Defects
hematopoietic defects
vasculature
Hematological Research
Hematopoietic Defects
Research Tools
Cancer Research
endothelial cell marker for neovascularization
Cardiovascular Research
Cre-lox System
endothelial cell marker for neovascularization
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: endothelial cell markers for neovascularization
Hematological Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cdh5-cre)7Mlia | ||
|---|---|---|---|
| Allele Name | transgene insertion 7, M Luisa Iruela-Arispe | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(Cdh5-cre)1Mlia; VE-Cadherin-Cre; VEC-Cre; VEN7-CRE; | ||
| Mutation Made By | M. Luisa Iruela-Arispe, University of California, Los Angeles | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Embryonic and adult expression in endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cdh5, cadherin 5, mouse, laboratory | ||
| Driver Note | Cdh5 | ||
| Molecular Note | The cadherin 5 promoter was used to drive expression of cre recombinase in the adult epithelium. Three lines were created. [MGI Ref ID J:106155] | ||
| Gene Symbol and Name | Tg(Cdh5-cre)7Mlia, transgene insertion 7, M Luisa Iruela-Arispe | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Cdh5-cre)1Mlia; VE-Cadherin-Cre; VEC-Cre; VEN7-CRE; transgene insertion 1, M Luisa Iruela-Arispe; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Alva JA; Zovein AC; Monvoisin A; Murphy T; Salazar A; Harvey NL; Carmeliet P; Iruela-Arispe ML. 2006. VE-Cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells. Dev Dyn 235(3):759-67. [PubMed: 16450386] [MGI Ref ID J:106155]
Additional References
Tg(Cdh5-cre)7Mlia relatedCorada M; Nyqvist D; Orsenigo F; Caprini A; Giampietro C; Taketo MM; Iruela-Arispe ML; Adams RH; Dejana E. 2010. The Wnt/beta-catenin pathway modulates vascular remodeling and specification by upregulating Dll4/Notch signaling. Dev Cell 18(6):938-49. [PubMed: 20627076] [MGI Ref ID J:163848]
Economopoulou M; Langer HF; Celeste A; Orlova VV; Choi EY; Ma M; Vassilopoulos A; Callen E; Deng C; Bassing CH; Boehm M; Nussenzweig A; Chavakis T. 2009. Histone H2AX is integral to hypoxia-driven neovascularization. Nat Med 15(5):553-8. [PubMed: 19377486] [MGI Ref ID J:149487]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Guo W; Lasky JL; Chang CJ; Mosessian S; Lewis X; Xiao Y; Yeh JE; Chen JY; Iruela-Arispe ML; Varella-Garcia M; Wu H. 2008. Multi-genetic events collaboratively contribute to Pten-null leukaemia stem-cell formation. Nature 453(7194):529-33. [PubMed: 18463637] [MGI Ref ID J:135172]
Guo W; Schubbert S; Chen JY; Valamehr B; Mosessian S; Shi H; Dang NH; Garcia C; Theodoro MF; Varella-Garcia M; Wu H. 2011. Suppression of leukemia development caused by PTEN loss. Proc Natl Acad Sci U S A 108(4):1409-14. [PubMed: 21212363] [MGI Ref ID J:168259]
Harel I; Nathan E; Tirosh-Finkel L; Zigdon H; Guimaraes-Camboa N; Evans SM; Tzahor E. 2009. Distinct origins and genetic programs of head muscle satellite cells. Dev Cell 16(6):822-32. [PubMed: 19531353] [MGI Ref ID J:150129]
Hofmann JJ; Zovein AC; Koh H; Radtke F; Weinmaster G; Iruela-Arispe ML. 2010. Jagged1 in the portal vein mesenchyme regulates intrahepatic bile duct development: insights into Alagille syndrome. Development 137(23):4061-72. [PubMed: 21062863] [MGI Ref ID J:166890]
Langer HF; Orlova VV; Xie C; Kaul S; Schneider D; Lonsdorf AS; Fahrleitner M; Choi EY; Dutoit V; Pellegrini M; Grossklaus S; Nawroth PP; Baretton G; Santoso S; Hwang ST; Arnold B; Chavakis T. 2011. A Novel Function of Junctional Adhesion Molecule-C in Mediating Melanoma Cell Metastasis. Cancer Res 71(12):4096-4105. [PubMed: 21593193] [MGI Ref ID J:173391]
Lee S; Chen TT; Barber CL; Jordan MC; Murdock J; Desai S; Ferrara N; Nagy A; Roos KP; Iruela-Arispe ML. 2007. Autocrine VEGF signaling is required for vascular homeostasis. Cell 130(4):691-703. [PubMed: 17719546] [MGI Ref ID J:124900]
Monvoisin A; Alva JA; Hofmann JJ; Zovein AC; Lane TF; Iruela-Arispe ML. 2006. VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium. Dev Dyn 235(12):3413-22. [PubMed: 17072878] [MGI Ref ID J:116638]
Myers SA; DeVries WH; Andres KR; Gruenthal MJ; Benton RL; Hoying JB; Hagg T; Whittemore SR. 2011. CD47 knockout mice exhibit improved recovery from spinal cord injury. Neurobiol Dis 42(1):21-34. [PubMed: 21168495] [MGI Ref ID J:170731]
Satyanarayana A; Gudmundsson KO; Chen X; Coppola V; Tessarollo L; Keller JR; Hou SX. 2010. RapGEF2 is essential for embryonic hematopoiesis but dispensable for adult hematopoiesis. Blood 116(16):2921-31. [PubMed: 20595512] [MGI Ref ID J:165879]
Skuli N; Liu L; Runge A; Wang T; Yuan L; Patel S; Iruela-Arispe L; Simon MC; Keith B. 2009. Endothelial deletion of hypoxia-inducible factor-2alpha (HIF-2alpha) alters vascular function and tumor angiogenesis. Blood 114(2):469-77. [PubMed: 19439736] [MGI Ref ID J:150753]
Tang Y; Harrington A; Yang X; Friesel RE; Liaw L. 2010. The contribution of the Tie2+ lineage to primitive and definitive hematopoietic cells. Genesis 48(9):563-7. [PubMed: 20645309] [MGI Ref ID J:170016]
Zhong Z; Ilieva H; Hallagan L; Bell R; Singh I; Paquette N; Thiyagarajan M; Deane R; Fernandez JA; Lane S; Zlokovic AB; Liu T; Griffin JH; Chow N; Castellino FJ; Stojanovic K; Cleveland DW; Zlokovic BV. 2009. Activated protein C therapy slows ALS-like disease in mice by transcriptionally inhibiting SOD1 in motor neurons and microglia cells. J Clin Invest 119(11):3437-49. [PubMed: 19841542] [MGI Ref ID J:154597]
Zovein AC; Hofmann JJ; Lynch M; French WJ; Turlo KA; Yang Y; Becker MS; Zanetta L; Dejana E; Gasson JC; Tallquist MD; Iruela-Arispe ML. 2008. Fate tracing reveals the endothelial origin of hematopoietic stem cells. Cell Stem Cell 3(6):625-36. [PubMed: 19041779] [MGI Ref ID J:149863]
Zovein AC; Luque A; Turlo KA; Hofmann JJ; Yee KM; Becker MS; Fassler R; Mellman I; Lane TF; Iruela-Arispe ML. 2010. Beta1 integrin establishes endothelial cell polarity and arteriolar lumen formation via a Par3-dependent mechanism. Dev Cell 18(1):39-51. [PubMed: 20152176] [MGI Ref ID J:157920]
Zovein AC; Turlo KA; Ponec RM; Lynch MR; Chen KC; Hofmann JJ; Cox TC; Gasson JC; Iruela-Arispe ML. 2010. Vascular remodeling of the vitelline artery initiates extravascular emergence of hematopoietic clusters. Blood 116(18):3435-44. [PubMed: 20699440] [MGI Ref ID J:166646]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings, C57BL/6J, or bred together to generate homozygous colonies. Mating System Homozygote x Homozygote (Female x Male) 05-SEP-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $225.00 Female or Male Homozygous for Tg(Cdh5-cre)7Mlia
Pairs /Price (US dollars $) Pair Genotype $450.00 Homozygous for Tg(Cdh5-cre)7Mlia x Homozygous for Tg(Cdh5-cre)7Mlia Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $292.50 Female or Male Homozygous for Tg(Cdh5-cre)7Mlia
Pairs /Price (US dollars $) Pair Genotype $585.00 Homozygous for Tg(Cdh5-cre)7Mlia x Homozygous for Tg(Cdh5-cre)7Mlia Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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