Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Richard Hodes,   NCI/NIH

Description
Hemizygous transgenic mice are viable, fertile, and display no gross anatomical or behavioral abnormalities. This "hTERT" transgene carries the complete human telomerase reverse transcriptase (TERT) gene, including at least 11 kb of upstream and 1.5 kb of downstream sequences. The tissue specificity of transgene expression in hemizygous mice recapitulates that of the endogenous hTERT in humans rather than that of the endogenous mouse Tert (mTERT) gene with one exception: in brain, hTERT expression follows the expression profile of the endogenous mTERT gene. These transgenic mice may be useful in studies of telomerase function, organ-specific, differential cis-regulation of mouse versus human genes, as well as in studies of DNA replication and repair mechanisms, human aging, and carcinogenesis.

Development
A transgene containing the 54-kb human TERT gene (including an at least 11-kb upstream region, all of the exons and introns, and 1.2-kb downstream region) was microinjected into C57BL/6 oocytes. Mice carrying the transgene (founder line BAC-C10) were bred to C57BL/6 for "several generations" and then intercrossed for more than 5 generations prior to arrival at The Jackson Laboratory.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(TERT)C10Hode/0

        involves: C57BL/6 * SPRET/Ei

• cellular phenotype
• abnormal telomere length (MGI Ref ID J:92243)
  • there is no difference in telomere elongation compared to wild-type B6 x SPRET/Ei mice; telomere length is not affected by transgene

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Other (DNA Repair)

Cell Biology Research
DNA Damage Response
Genes Regulating Growth and Proliferation
Transcriptional Regulation

Research Tools
Cancer Research
Cell Biology Research
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(TERT)C10Hode
Allele Name		transgene insertion C10, Richard J Hodes
Allele Type		Transgenic (random, expressed)
Common Name(s)		BAC-C10; hTERT; hTERT-BAC-C10; line BAC-C10;
Mutation Made By		Richard Hodes,   NCI/NIH
Strain of Origin		C57BL/6
Expressed Gene		TERT, telomerase reverse transcriptase, human
Promoter		TERT, telomerase reverse transcriptase, human
General Note		Mice of two independent transgenic founder lines, designated BAC-C2 and BAC-C10, were crossed with mice heterozygous for a targeted mutation of the endogenous mouse Tert gene to produce lines segregating for the transgene and the targeted mutation. Expression analyses were performed on mice of various genotypes from both lines. Most data presented were from line BAC-C10.
Molecular Note		A linearized bacterial artificial chromosome (BAC) containing the 54-kb human telomerase reverse transcriptase gene, including at least 11 kb of upstream sequence, all of the exons and introns, and 1.2 kb of downstream sequence, was microinjected into C57BL/6 oocytes. Transcript expression patterns of the human gene in most organs of transgenic mice are generally characteristic of TERT expression in humans, rather than of the endogenous mouse gene; the exception is that human TERT, which is not expressed in human brain, is like the mouse enzyme expressed in mouse brain. [MGI Ref ID J:117922]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(TERT)C10Hode, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Horikawa I; Chiang YJ; Patterson T; Feigenbaum L; Leem SH; Michishita E; Larionov V; Hodes RJ; Barrett JC. 2005. Differential cis-regulation of human versus mouse TERT gene expression in vivo: identification of a human-specific repressive element. Proc Natl Acad Sci U S A 102(51):18437-42. [PubMed: 16344462]  [MGI Ref ID J:117922]

Additional References

Tg(TERT)C10Hode related

Chiang YJ; Hemann MT; Hathcock KS; Tessarollo L; Feigenbaum L; Hahn WC; Hodes RJ. 2004. Expression of telomerase RNA template, but not telomerase reverse transcriptase, is limiting for telomere length maintenance in vivo. Mol Cell Biol 24(16):7024-31. [PubMed: 15282303]  [MGI Ref ID J:92243]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, transgenic mice can be bred to wildtype siblings (or C57BL/6J). Alternatively, transgenic mice can be bred together.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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