| |||||||||||||||||||
| Cre activity in hemizygous mice is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x +/+ sibling (Female x Male) 16-APR-08 Species laboratory mouse Generation N12+N2F15 (18-OCT-12)
Generation DefinitionsDonating Investigator Fred Van Leuven, Katholieke Universiteit Leuven Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease.View cre expression characterization.
Development
A recombinant vector was designed to place cre recombinase cDNA under control of the mouse Thy1 gene promoter (Thy1.2 sequence). This construct was microinjected into FVB/N pre-nuclear embryos (0.5 day old). A selected founder was bred to FVB/N mice and backcrossed to the same for more than 7 generations. These mice, originally developed by Dr. Fred Van Leuven of The Katholieke Universiteit (Leuven, Belgium) were sent to The Jackson Laboratory from GIGA Transgenics Facility (Liege, Belgium) as hemizygotes in 2006. Upon arrival, mutant mice were bred to FVB/NJ mice for one generation and are now maintained by breeding transgenic mice with FVB/NJ or wildtype siblings.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
Additional Web Information
Introduction to Cre-lox technology
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Cortical Defects
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-cre)1Vln | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Fred Van Leuven | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | thy1-Cre; | ||
| Mutation Made By | Fred Van Leuven, Katholieke Universiteit Leuven | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | neurons of the postnatal cortex and hippocampus | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Thy1-cre)1Vln, transgene insertion 1, Fred Van Leuven | ||
| Chromosome | UN | ||
| Gene Common Name(s) | thy1-Cre; | ||
| Driver Note | Thy1 | ||
| Molecular Note | Sequence encoding cre recombinase was adjoined to the mouse Thy1 promoter. Expression was driven in neurons of the postnatal cortex and hippocampus. [MGI Ref ID J:87229] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Dewachter I; Reverse D; Caluwaerts N; Ris L; Kuiperi C; Van den Haute C; Spittaels K; Umans L; Serneels L; Thiry E; Moechars D; Mercken M; Godaux E; Van Leuven F. 2002. Neuronal deficiency of presenilin 1 inhibits amyloid plaque formation and corrects hippocampal long-term potentiation but not a cognitive defect of amyloid precursor protein [V717I] transgenic mice. J Neurosci 22(9):3445-53. [PubMed: 11978821] [MGI Ref ID J:87229]
Additional References
Tg(Thy1-cre)1Vln relatedDewachter I; Ris L; Croes S; Borghgraef P; Devijver H; Voets T; Nilius B; Godaux E; Van Leuven F. 2008. Modulation of synaptic plasticity and Tau phosphorylation by wild-type and mutant presenilin1. Neurobiol Aging 29(5):639-52. [PubMed: 17222948] [MGI Ref ID J:137684]
Fuerst PG; Bruce F; Rounds RP; Erskine L; Burgess RW. 2012. Cell autonomy of DSCAM function in retinal development. Dev Biol 361(2):326-37. [PubMed: 22063212] [MGI Ref ID J:179393]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Herms J; Schneider I; Dewachter I; Caluwaerts N; Kretzschmar H; Van Leuven F. 2003. Capacitive calcium entry is directly attenuated by mutant presenilin-1, independent of the expression of the amyloid precursor protein. J Biol Chem 278(4):2484-9. [PubMed: 12431992] [MGI Ref ID J:81726]
Ivanova E; Hwang GS; Pan ZH. 2010. Characterization of transgenic mouse lines expressing Cre recombinase in the retina. Neuroscience 165(1):233-43. [PubMed: 19837136] [MGI Ref ID J:158209]
Liang X; Lin L; Woodling NS; Wang Q; Anacker C; Pan T; Merchant M; Andreasson K. 2011. Signaling via the prostaglandin E receptor EP4 exerts neuronal and vascular protection in a mouse model of cerebral ischemia. J Clin Invest 121(11):4362-71. [PubMed: 21965326] [MGI Ref ID J:178464]
Moore DL; Blackmore MG; Hu Y; Kaestner KH; Bixby JL; Lemmon VP; Goldberg JL. 2009. KLF family members regulate intrinsic axon regeneration ability. Science 326(5950):298-301. [PubMed: 19815778] [MGI Ref ID J:153499]
Rada P; Rojo AI; Evrard-Todeschi N; Innamorato NG; Cotte A; Jaworski T; Tobon-Velasco JC; Devijver H; Garcia-Mayoral MF; Van Leuven F; Hayes JD; Bertho G; Cuadrado A. 2012. Structural and functional characterization of Nrf2 degradation by the glycogen synthase kinase 3/beta-TrCP axis. Mol Cell Biol 32(17):3486-99. [PubMed: 22751928] [MGI Ref ID J:188804]
Ris L; Dewachter I; Reverse D; Godaux E; Van Leuven F. 2003. Capacitative calcium entry induces hippocampal long term potentiation in the absence of presenilin-1. J Biol Chem 278(45):44393-9. [PubMed: 12902342] [MGI Ref ID J:86553]
Tamboli IY; Prager K; Thal DR; Thelen KM; Dewachter I; Pietrzik CU; St George-Hyslop P; Sisodia SS; De Strooper B; Heneka MT; Filippov MA; Muller U; van Leuven F; Lutjohann D; Walter J. 2008. Loss of gamma-secretase function impairs endocytosis of lipoprotein particles and membrane cholesterol homeostasis. J Neurosci 28(46):12097-106. [PubMed: 19005074] [MGI Ref ID J:142399]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry Although the donating investigator reports homozygous mice are viable and fertile, transgenic carriers are bred to wildtype sibling or FVB/NJ mice at The Jackson Laboratory. Mating System Hemizygote x +/+ sibling (Female x Male) 16-APR-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Thy1-cre)1Vln
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Thy1-cre)1Vln x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Thy1-cre)1Vln Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Thy1-cre)1Vln
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Thy1-cre)1Vln x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Thy1-cre)1Vln Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.