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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Hemizygote x +/+ sibling (Female x Male) 16-APR-08 Species laboratory mouse Generation N12+N2F5 (03-DEC-08) Donating Investigator Fred Van Leuven, Katholieke Universiteit Leuven Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease.View cre expression characterization.
Development
A recombinant vector was designed to place cre recombinase cDNA under control of the mouse Thy1 gene promoter (Thy1.2 sequence). This construct was microinjected into FVB/N pre-nuclear embryos (0.5 day old). A selected founder was bred to FVB/N mice and backcrossed to the same for more than 7 generations. These mice, originally developed by Dr. Fred Van Leuven of The Katholieke Universiteit (Leuven, Belgium) were sent to The Jackson Laboratory from GIGA Transgenics Facility (Liege, Belgium) as hemizygotes in 2006. Upon arrival, mutant mice were bred to FVB/NJ mice for one generation and are now maintained by breeding transgenic mice with FVB/NJ or wildtype siblings.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Thy1
View Strains carrying other alleles of Thy1 (43 strains)
Additional Web Information
Introduction to Cre-lox technology
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Cortical Defects
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Tissue/Cell Markers: neurons
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-cre)1Vln | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Fred Van Leuven | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | thy1-Cre; | ||
| Mutation Made By | Fred Van Leuven, Katholieke Universiteit Leuven | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | neurons of the postnatal cortex and hippocampus | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Driver Note | Thy1 | ||
| Molecular Note | Sequence encoding cre recombinase was adjoined to the mouse Thy1 promoter. Expression was driven in neurons of the postnatal cortex and hippocampus. [MGI Ref ID J:87229] | ||
| Gene Symbol and Name | Tg(Thy1-cre)1Vln, transgene insertion 1, Fred Van Leuven | ||
| Chromosome | UN | ||
| Gene Common Name(s) | thy1-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Dewachter I; Reverse D; Caluwaerts N; Ris L; Kuiperi C; Van den Haute C; Spittaels K; Umans L; Serneels L; Thiry E; Moechars D; Mercken M; Godaux E; Van Leuven F. 2002. Neuronal deficiency of presenilin 1 inhibits amyloid plaque formation and corrects hippocampal long-term potentiation but not a cognitive defect of amyloid precursor protein [V717I] transgenic mice. J Neurosci 22(9):3445-53. [PubMed: 11978821] [MGI Ref ID J:87229]
Additional References
Tg(Thy1-cre)1Vln relatedDewachter I; Ris L; Croes S; Borghgraef P; Devijver H; Voets T; Nilius B; Godaux E; Van Leuven F. 2008. Modulation of synaptic plasticity and Tau phosphorylation by wild-type and mutant presenilin1. Neurobiol Aging 29(5):639-52. [PubMed: 17222948] [MGI Ref ID J:137684]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Herms J; Schneider I; Dewachter I; Caluwaerts N; Kretzschmar H; Van Leuven F. 2003. Capacitive calcium entry is directly attenuated by mutant presenilin-1, independent of the expression of the amyloid precursor protein. J Biol Chem 278(4):2484-9. [PubMed: 12431992] [MGI Ref ID J:81726]
Moore DL; Blackmore MG; Hu Y; Kaestner KH; Bixby JL; Lemmon VP; Goldberg JL. 2009. KLF family members regulate intrinsic axon regeneration ability. Science 326(5950):298-301. [PubMed: 19815778] [MGI Ref ID J:153499]
Ris L; Dewachter I; Reverse D; Godaux E; Van Leuven F. 2003. Capacitative calcium entry induces hippocampal long term potentiation in the absence of presenilin-1. J Biol Chem 278(45):44393-9. [PubMed: 12902342] [MGI Ref ID J:86553]
Tamboli IY; Prager K; Thal DR; Thelen KM; Dewachter I; Pietrzik CU; St George-Hyslop P; Sisodia SS; De Strooper B; Heneka MT; Filippov MA; Muller U; van Leuven F; Lutjohann D; Walter J. 2008. Loss of gamma-secretase function impairs endocytosis of lipoprotein particles and membrane cholesterol homeostasis. J Neurosci 28(46):12097-106. [PubMed: 19005074] [MGI Ref ID J:142399]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry Although the donating investigator reports homozygous mice are viable and fertile, transgenic carriers are bred to wildtype sibling or FVB/NJ mice at The Jackson Laboratory. Mating System Hemizygote x +/+ sibling (Female x Male) 16-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Thy1-cre)1Vln
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Thy1-cre)1Vln x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Thy1-cre)1Vln
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Thy1-cre)1Vln
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Thy1-cre)1Vln x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Thy1-cre)1Vln
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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