Strain Name: |
FVB/N-Tg(Thy1-cre)1Vln/J |
|---|---|
Stock Number: |
006143 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Thy1; cre; Tg(Thy1-cre)1Vln; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Fred Van Leuven, Katholieke Universiteit Leuven Generation N12+N2F2 (05-DEC-07) Strain Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease.Strain Development
A recombinant vector was designed to place cre recombinase cDNA under control of the mouse Thy1 gene promoter (Thy1.2 sequence). This construct was microinjected into FVB/N pre-nuclear embryos (0.5 day old). A selected founder was bred to FVB/N mice and backcrossed to the same for more than 7 generations. These mice, originally developed by Dr. Fred Van Leuven of The Katholieke Universiteit (Leuven, Belgium) were sent to The Jackson Laboratory from GIGA Transgenics Facility (Liege, Belgium) as hemizygotes in 2006. Upon arrival, mutant mice were bred to FVB/NJ mice for one generation and are now maintained by breeding transgenic mice with FVB/NJ or wildtype siblings.
Gene & Allele Details
| Allele Symbol | Tg(Thy1-cre)1Vln | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Fred Van Leuven | ||
| Common Name(s) | thy1-Cre; | ||
| Mutation Made By | Fred Van Leuven, Katholieke Universiteit Leuven | ||
| Site of Expression | neurons of the postnatal cortex and hippocampus | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | Sequence encoding cre recombinase was adjoined to the mouse Thy1 promoter. Expression was driven in neurons of the postnatal cortex and hippocampus. [MGI Ref ID J:87229] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | Although the donating investigator reports homozygous mice are viable and fertile, transgenic carriers are bred to wildtype sibling or FVB/NJ mice at The Jackson Laboratory. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Thy1
View Strains carrying other alleles of Thy1 (37 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
Cortical Defects
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Tissue/Cell Markers: neurons)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesDewachter I; Reverse D; Caluwaerts N; Ris L; Kuiperi C; Van den Haute C; Spittaels K; Umans L; Serneels L; Thiry E; Moechars D; Mercken M; Godaux E; Van Leuven F. 2002. Neuronal deficiency of presenilin 1 inhibits amyloid plaque formation and corrects hippocampal long-term potentiation but not a cognitive defect of amyloid precursor protein [V717I] transgenic mice. J Neurosci 22(9):3445-53. [PubMed: 11978821] [MGI Ref ID J:87229]
Price and Supply Information
| Strain Name: | FVB/N-Tg(Thy1-cre)1Vln/J |
| Stock Number: | 006143 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Thy1-cre)1Vln | |||
| Pair | $375.30 | Hemizygous for Tg(Thy1-cre)1Vln x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Thy1-cre)1Vln | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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